Structured Review

Thermo Fisher pentr5
Pentr5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentr5/product/Thermo Fisher
Average 88 stars, based on 7 article reviews
Price from $9.99 to $1999.99
pentr5 - by Bioz Stars, 2020-09
88/100 stars

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Related Articles

Clone Assay:

Article Title: Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability
Article Snippet: .. Lentiviral infections EB2-WT and mutant genes cloned into pENTR/D-TOPO and pENTR5'/EF1αp were co-transferred into the pcLenti6.4/R4R2/V5-DEST vector (Gateway Technology, Thermo Fisher). ..

Article Title: A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation
Article Snippet: .. Fragments were cloned into pENTR5’ (Life Technologies) per manufacturers instructions. .. Expression vectors were derived using Multisite Gateway® technology per manufacturers specifications and the Tol2 Kit ( ) ( EGFP , vector 383; mCh , vector 386; SV40 poly A, vector 302; EGFP with mouse minimal β-globin promoter ( ); destination vectors, vector 394 alone and modified with addition of crystallin:YFP marker; zmitfa middle entry vector, gift of Craig Ceol; MiniCoopR from ( )).

Expressing:

Article Title: Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183
Article Snippet: .. For stable V5-tagged protein expression, the entry clone product and pENTR5’/CMVp vector (Invitrogen) were recombined into the pLenti 6.4/R4R2/V5-DEST vector (Invitrogen) using LR Clonase II Plus enzyme mix (Invitrogen). .. To produce lentivirus particles expressing V5-tagged mouse RNF183 or RNF152, the pLenti-based expression vector and ViraPower Packaging mix (Invitrogen) were cotransfected into the 293FT cell line.

Mutagenesis:

Article Title: Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability
Article Snippet: .. Lentiviral infections EB2-WT and mutant genes cloned into pENTR/D-TOPO and pENTR5'/EF1αp were co-transferred into the pcLenti6.4/R4R2/V5-DEST vector (Gateway Technology, Thermo Fisher). ..

BAC Assay:

Article Title: Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Article Snippet: .. Use PCR to amplify the Per2 promoter DNA fragment of 526 bp, upstream of the transcription start site from a mouse Per2 BAC clone9-13 , using a forward primer (5'-CTCGAGCGGATTACCGAGGCTGGTCACG TC-3') and a reverse primer (5'-CTCGAGTCCCTTGCTCGGCCCGTCAC TTGG-3'), and clone into pENTR5'-TOPO vector (Invitrogen) to generate pENTR5'-P( Per2 ). .. Cloning of d Luc .

Polymerase Chain Reaction:

Article Title: Active receptor tyrosine kinases, but not Brachyury, are sufficient to trigger chordoma in zebrafish
Article Snippet: .. The resulting PCR product was then TA-cloned into pENTR5′ (Thermo Fisher Scientific) according to the manufacturer's instructions. .. The mouse β-Globin minimal promoter (minprom) was amplified from pME-minprom-EGFP ( ) with the primers mouse β-Globin minimal promoter BamHI forward and mouse β-Globin minimal promoter XbaI reverse and cloned into pENTR5′-col2a1aR2.

Article Title: Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Article Snippet: .. Use PCR to amplify the Per2 promoter DNA fragment of 526 bp, upstream of the transcription start site from a mouse Per2 BAC clone9-13 , using a forward primer (5'-CTCGAGCGGATTACCGAGGCTGGTCACG TC-3') and a reverse primer (5'-CTCGAGTCCCTTGCTCGGCCCGTCAC TTGG-3'), and clone into pENTR5'-TOPO vector (Invitrogen) to generate pENTR5'-P( Per2 ). .. Cloning of d Luc .

Plasmid Preparation:

Article Title: Sec16A, a key protein in COPII vesicle formation, regulates the stability and localization of the novel ubiquitin ligase RNF183
Article Snippet: .. For stable V5-tagged protein expression, the entry clone product and pENTR5’/CMVp vector (Invitrogen) were recombined into the pLenti 6.4/R4R2/V5-DEST vector (Invitrogen) using LR Clonase II Plus enzyme mix (Invitrogen). .. To produce lentivirus particles expressing V5-tagged mouse RNF183 or RNF152, the pLenti-based expression vector and ViraPower Packaging mix (Invitrogen) were cotransfected into the 293FT cell line.

Article Title: Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability
Article Snippet: .. Lentiviral infections EB2-WT and mutant genes cloned into pENTR/D-TOPO and pENTR5'/EF1αp were co-transferred into the pcLenti6.4/R4R2/V5-DEST vector (Gateway Technology, Thermo Fisher). ..

Article Title: Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Article Snippet: .. Use PCR to amplify the Per2 promoter DNA fragment of 526 bp, upstream of the transcription start site from a mouse Per2 BAC clone9-13 , using a forward primer (5'-CTCGAGCGGATTACCGAGGCTGGTCACG TC-3') and a reverse primer (5'-CTCGAGTCCCTTGCTCGGCCCGTCAC TTGG-3'), and clone into pENTR5'-TOPO vector (Invitrogen) to generate pENTR5'-P( Per2 ). .. Cloning of d Luc .

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    Thermo Fisher pentr5
    Pentr5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr5/product/Thermo Fisher
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pentr5 - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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