Structured Review

Addgene inc pentr5 ubi
Pentr5 Ubi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentr5 ubi/product/Addgene inc
Average 93 stars, based on 8 article reviews
Price from $9.99 to $1999.99
pentr5 ubi - by Bioz Stars, 2020-09
93/100 stars

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Related Articles

Clone Assay:

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B ( ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site ( Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B (ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site (Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Amplification:

Article Title: Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis
Article Snippet: .. To generate pME-loxP-eGFP-pA-loxP (referred to as LGSL ), the cassette was amplified from pENTR5′_ubi:loxP-EGFP-loxP (Addgene plasmid no. 27322) ( ) and recombined into pDONR221. pME-loxP-mCerulean-pA-loxP was generated by replacing the eGFP ORF in pME-loxP-eGFP-pA-loxP with mCerulean via Not I/Nco I digestion and ligation. .. Plasmids were sequence verified before downstream assembly.

Article Title: Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320). pENTR5′_ubi was digested by XhoI and BamHI, and the lyzC promoter was amplified with primers p5E-lyzC-F/R. .. In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the lyzC fragment with the linearized backbone.

Article Title: Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology
Article Snippet: .. Ubiquitin promoter was amplified by PCR from pENTR5′_ubi using Q5® High-Fidelity DNA Polymerase, with these primers (F: 5′-cattgaGCTAGCatggatgttttcccagtcacgacg-3′, R:5′-tgactaGGATCCtgtaaacaaattcaaagtaagat-3′) and the following thermocycling protocol: 98 °C 30′′ (98 °C 10′′, 52 °C 30′′, 72 °C 2′) X35 cycles 72 °C 2′ pENTR5′_ubi was a gift from Leonard Zon (Addgene plasmid # 27320). .. The PCR product was ran on an agarose gel and the band-purified using Wizard® SV Gel and PCR Clean-Up kit from Promega.

Article Title: Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320). pENTR5′_ubi was digested by XhoI and BamHI, and the lyzC promoter was amplified with primers p5E-lyzC-F/R. .. In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the lyzC fragment with the linearized backbone.

Ligation:

Article Title: Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis
Article Snippet: .. To generate pME-loxP-eGFP-pA-loxP (referred to as LGSL ), the cassette was amplified from pENTR5′_ubi:loxP-EGFP-loxP (Addgene plasmid no. 27322) ( ) and recombined into pDONR221. pME-loxP-mCerulean-pA-loxP was generated by replacing the eGFP ORF in pME-loxP-eGFP-pA-loxP with mCerulean via Not I/Nco I digestion and ligation. .. Plasmids were sequence verified before downstream assembly.

Modification:

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B ( ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site ( Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B (ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site (Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Generated:

Article Title: Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis
Article Snippet: .. To generate pME-loxP-eGFP-pA-loxP (referred to as LGSL ), the cassette was amplified from pENTR5′_ubi:loxP-EGFP-loxP (Addgene plasmid no. 27322) ( ) and recombined into pDONR221. pME-loxP-mCerulean-pA-loxP was generated by replacing the eGFP ORF in pME-loxP-eGFP-pA-loxP with mCerulean via Not I/Nco I digestion and ligation. .. Plasmids were sequence verified before downstream assembly.

Article Title: Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320). pENTR5′_ubi was digested by XhoI and BamHI, and the lyzC promoter was amplified with primers p5E-lyzC-F/R. .. In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the lyzC fragment with the linearized backbone.

Article Title: Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320). pENTR5′_ubi was digested by XhoI and BamHI, and the lyzC promoter was amplified with primers p5E-lyzC-F/R. .. In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the lyzC fragment with the linearized backbone.

Article Title: A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320) as described . .. The pME-Cas9 plasmid is from Addgene (Addgene #63154).

Derivative Assay:

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B ( ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site ( Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B (ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site (Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Polymerase Chain Reaction:

Article Title: Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology
Article Snippet: .. Ubiquitin promoter was amplified by PCR from pENTR5′_ubi using Q5® High-Fidelity DNA Polymerase, with these primers (F: 5′-cattgaGCTAGCatggatgttttcccagtcacgacg-3′, R:5′-tgactaGGATCCtgtaaacaaattcaaagtaagat-3′) and the following thermocycling protocol: 98 °C 30′′ (98 °C 10′′, 52 °C 30′′, 72 °C 2′) X35 cycles 72 °C 2′ pENTR5′_ubi was a gift from Leonard Zon (Addgene plasmid # 27320). .. The PCR product was ran on an agarose gel and the band-purified using Wizard® SV Gel and PCR Clean-Up kit from Promega.

Plasmid Preparation:

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B ( ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site ( Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Article Title: Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis
Article Snippet: .. To generate pME-loxP-eGFP-pA-loxP (referred to as LGSL ), the cassette was amplified from pENTR5′_ubi:loxP-EGFP-loxP (Addgene plasmid no. 27322) ( ) and recombined into pDONR221. pME-loxP-mCerulean-pA-loxP was generated by replacing the eGFP ORF in pME-loxP-eGFP-pA-loxP with mCerulean via Not I/Nco I digestion and ligation. .. Plasmids were sequence verified before downstream assembly.

Article Title: Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320). pENTR5′_ubi was digested by XhoI and BamHI, and the lyzC promoter was amplified with primers p5E-lyzC-F/R. .. In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the lyzC fragment with the linearized backbone.

Article Title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
Article Snippet: .. The pT2TS-ubb:Cas9;u6c:Dr-tRNAGly (GCC)-sgRNA-scaffold consists of zebrafish ubiquitin B (ubb ) promoter excised from pENTR5′_ubi (Addgene plasmid 27320), hSpCas9 derived from pCS2 + hSpCas9 (Addgene plasmid 51815), poly-A addition signal, zebrafish U6c promoter , zebrafish tRNAGly (GCC), a cloning site (Bsa I and Bse RI) for multiple tRNA-sgRNAs, and a modified version of sgRNA-scaffold . .. These DNA fragments were artificially synthesized unless otherwise stated and were cloned between the Sal I and Hin dIII restriction sites of pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 (Addgene plasmid 74009).

Article Title: Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology
Article Snippet: .. Ubiquitin promoter was amplified by PCR from pENTR5′_ubi using Q5® High-Fidelity DNA Polymerase, with these primers (F: 5′-cattgaGCTAGCatggatgttttcccagtcacgacg-3′, R:5′-tgactaGGATCCtgtaaacaaattcaaagtaagat-3′) and the following thermocycling protocol: 98 °C 30′′ (98 °C 10′′, 52 °C 30′′, 72 °C 2′) X35 cycles 72 °C 2′ pENTR5′_ubi was a gift from Leonard Zon (Addgene plasmid # 27320). .. The PCR product was ran on an agarose gel and the band-purified using Wizard® SV Gel and PCR Clean-Up kit from Promega.

Article Title: Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320). pENTR5′_ubi was digested by XhoI and BamHI, and the lyzC promoter was amplified with primers p5E-lyzC-F/R. .. In-Fusion cloning (In-Fusion HD Cloning Plus Kit, Clontech) was used to fuse the lyzC fragment with the linearized backbone.

Article Title: A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish
Article Snippet: .. The p5E-lyzC entry vector was generated by replacing the ubiquitin promoter with the lyzC promoter in pENTR5′_ubi (Addgene #27320) as described . .. The pME-Cas9 plasmid is from Addgene (Addgene #63154).

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    Addgene inc pentr5 ubi
    Pentr5 Ubi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr5 ubi/product/Addgene inc
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pentr5 ubi - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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