pentr u6 vector (Thermo Fisher)
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Name:
BLOCK iT U6 RNAi Entry Vector Kit
Description:
The BLOCK iT U6 RNAi Entry Vector provides a simple streamlined approach to cloning short hairpin RNA shRNA sequences for testing in transient transfections for RNA interference RNAi a preferred technique for analyzing gene downregulation An easy cloning process places an 50 bp oligonucleotide DNA immediately following a U6 pol III type promoter Figure 1 Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA siRNA that will generate the RNAi effect Delivery of the U6 RNAi CassetteOnce cloning is complete the U6 Entry Vector is ready to be used immediately in transient transfections using a reagent such as Lipofectamine 2000 This makes this system ideal for initial shRNA screenings in many mammalian cell types As an alternative in hard to transfect or non dividing cell types or to deliver to animal model systems the RNAi cassette can easily be recombined into a BLOCK iT Viral RNAi vector For stable shRNA lentiviral delivery and expression recombine the BLOCK iT U6 Entry Vector with the pLenti6 BLOCK iT RNAi Vector For transient delivery to challenging cell types using adenoviral transduction recombine with the pAd BLOCK iT RNAi Vector
Catalog Number:
k494500
Price:
None
Applications:
Cloning|Destination Vectors|Gateway Cloning|RNAi|RNAi, Epigenetics & Non-Coding RNA Research|Vector-Based RNAi
Category:
DNA Vectors Clones Purified Nucleic Acids Libraries
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Structured Review
Thermo Fisher
pentr u6 vector
The BLOCK iT U6 RNAi Entry Vector provides a simple streamlined approach to cloning short hairpin RNA shRNA sequences for testing in transient transfections for RNA interference RNAi a preferred technique for analyzing gene downregulation An easy cloning process places an 50 bp oligonucleotide DNA immediately following a U6 pol III type promoter Figure 1 Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA siRNA that will generate the RNAi effect Delivery of the U6 RNAi CassetteOnce cloning is complete the U6 Entry Vector is ready to be used immediately in transient transfections using a reagent such as Lipofectamine 2000 This makes this system ideal for initial shRNA screenings in many mammalian cell types As an alternative in hard to transfect or non dividing cell types or to deliver to animal model systems the RNAi cassette can easily be recombined into a BLOCK iT Viral RNAi vector For stable shRNA lentiviral delivery and expression recombine the BLOCK iT U6 Entry Vector with the pLenti6 BLOCK iT RNAi Vector For transient delivery to challenging cell types using adenoviral transduction recombine with the pAd BLOCK iT RNAi Vector
https://www.bioz.com/result/pentr u6 vector/product/Thermo Fisher
Average 99 stars, based on 58 article reviews
Price from $9.99 to $1999.99
The BLOCK iT U6 RNAi Entry Vector provides a simple streamlined approach to cloning short hairpin RNA shRNA sequences for testing in transient transfections for RNA interference RNAi a preferred technique for analyzing gene downregulation An easy cloning process places an 50 bp oligonucleotide DNA immediately following a U6 pol III type promoter Figure 1 Expression of this RNAi cassette forms a shRNA molecule in the cell that will be processed and act as short interfering RNA siRNA that will generate the RNAi effect Delivery of the U6 RNAi CassetteOnce cloning is complete the U6 Entry Vector is ready to be used immediately in transient transfections using a reagent such as Lipofectamine 2000 This makes this system ideal for initial shRNA screenings in many mammalian cell types As an alternative in hard to transfect or non dividing cell types or to deliver to animal model systems the RNAi cassette can easily be recombined into a BLOCK iT Viral RNAi vector For stable shRNA lentiviral delivery and expression recombine the BLOCK iT U6 Entry Vector with the pLenti6 BLOCK iT RNAi Vector For transient delivery to challenging cell types using adenoviral transduction recombine with the pAd BLOCK iT RNAi Vector
https://www.bioz.com/result/pentr u6 vector/product/Thermo Fisher
Average 99 stars, based on 58 article reviews
Price from $9.99 to $1999.99
pentr u6 vector - by Bioz Stars,
2021-01
99/100 stars
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Images
1) Product Images from "Gene silencing of EXTL2 and EXTL3 as a substrate deprivation therapy for heparan sulphate storing mucopolysaccharidoses"
Article Title: Gene silencing of EXTL2 and EXTL3 as a substrate deprivation therapy for heparan sulphate storing mucopolysaccharidoses
Journal: European Journal of Human Genetics
doi: 10.1038/ejhg.2009.143
Figure Legend Snippet: Reduction in gag synthesis. 293T cells were transfected with shEXTL2.1, shEXTL2.2, shEXTL2.3, shEXTL3.2 or ctrlEXTL expressed from the pENTR/U6 vector. A mock transfection consisting of lipofectamine 2000 reagent only served as a control. After 48 h, 5 μCi/ml 35 SO 4 was added and gag synthesis determined over 4 h. Results are the mean±standard deviation of three replicates, and are expressed as the percentage of the mock control. * Significant difference from mock transfection, P
Techniques Used: Transfection, Plasmid Preparation, Standard Deviation
Figure Legend Snippet: Quantitative knockdown of EXTL2 and EXTL3 . 293T cells were transfected with psiCHECK-2EXTL2 or psiCHECK-2EXTL3 only, or co-transfected with a psiCHECK-2 target gene construct and corresponding shEXTL or ctrlEXTL expressed from the pENTR/U6 vector. Control cells were mock transfected with lipofectamine 2000 reagent only. A DLR assay was conducted 24 h post-transfection, and results expressed as a percentage of psiCHECK-2 target gene expression. Results are the mean±standard deviation of n =10 replicates. * Significant difference from mock transfection P
Techniques Used: Transfection, Construct, Plasmid Preparation, Expressing, Standard Deviation
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The oligonucleotides were synthesized by Nucleic Acids Core Facility at UNC, annealed and ligated into Article Title: Expression of long non‐coding RNA ENSG00000226738 (Lnc KLHDC7B) is enriched in the immunomodulatory triple‐negative breast cancer subtype and its alteration promotes cell migration, invasion, and resistance to cell death Article Snippet: .. 2.8 Long non‐coding RNA down‐regulation with shRNA Short hairpin RNA were generated using the |