pentr sd d topo vector  (Thermo Fisher)


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    Name:
    pENTR SD D TOPO Cloning Kit
    Description:
    The pENTR SD D TOPO Cloning Kits utilize a highly efficient 5 minute cloning strategy TOPO Cloning to directionally clone a blunt end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System Blunt end PCR products clone directionally into the pENTR SD D TOPO entry vector at greater than 90 efficiency and include an upstream Shine Dalgarno sequence for expression in prokaryotes The kit comes with everything necessary to clone and select your PCR amplified gene of interest • Gateway System Ready Rapidly shuttle cloned genes between multiple vector systems• Fast and Easy Go from PCR to Gateway Entry clone in just 3 steps and as little as 5 minutes hands on time• Efficient Achieve over 90 clones with correct insert in the right direction• Proven Reliable performance for over a decade pENTR SD D TOPO Cloning Kits Overview VECTOR pENTR SD D TOPO Vector Directional cloning vector for entry to the Gateway System and optimized for prokaryotic expression CLONING METHOD Directional TOPO Cloning Topoisomerase I based 5 minute directional ligation of blunt end proofreading polymerase amplified PCR products to the vector COMPETENT CELLS Two Options Choose from kits with either high efficiency or fast growing competent cells Simple Access to the Gateway SystemFor access to the Gateway System just PCR amplify your gene of interest and add the product straight to the provided topoisomerase charged pENTR SD D TOPO vector incubate 5 minutes and transform the provided competent E coli cells The resulting attL containing Gateway Entry clones are ready for efficient recombination with your choice of Gateway Destination vectors Optimized pENTR SD D TOPO VectorThe pENTR SD D TOPO vector Figure 1 includes a T7 gene 10 translational enhancer and a ribosome binding site RBS for optimal expression of native protein after recombination with a prokaryotic Gateway destination vectors You can also use the vector for expression in other hosts systems by recombination with different destination vectors The vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site This allows clones to be easily sequence verified and recombined into your choice of attR containing Gateway destination vectors A Kanamycin resistance gene and a pUC origin are used for selection and high copy propagation in E coli Simplified Directional CloningWith Directional TOPO cloning technology there is no need for PCR clean up vector preparation or other time intensive DNA manipulation steps Just add your PCR reaction straight to the provided topoisomerase charged vector incubate 5 minutes transform and obtain up to 90 directionally inserted clones A four base overhang on the vector pairs with a four base sequence designed into the forward primer used in your PCR reaction to provide directionality to the topoisomerase ligation reaction Figure 2 The Power of Gateway Recombination Cloning TechnologyGateway recombination cloning technology circumvents the limitations of restriction mediated cloning enabling you to access virtually any expression system in a simple one hour 99 efficient and reversible Gateway recombination reaction The ability to move the same sequence of DNA between different vectors without using restriction enzymes ligase subcloning steps screening of countless colonies or re sequencing will help save you time money and effort Leading Cloning TechnologiesWhen it comes to cloning TOPO cloning technology and Gateway recombination cloning technology have been a reliable partner for thousands of scientists for over ten years Fast simple to use and efficient TOPO cloning and Gateway recombination allow for rapid cloning and subsequent transferring of genes between a wide assortment of Gateway expression vectors Kit OptionsThe pENTR SD D TOPO Cloning Kit can be purchased with either TOP10 competent cells for standard cloning or Mach1 T1R competent cells for fast growth
    Catalog Number:
    k242020
    Price:
    None
    Applications:
    Cloning|Entry Vectors & Kits|Gateway Cloning|pENTR Vectors & Kits
    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Structured Review

    Thermo Fisher pentr sd d topo vector
    The pENTR SD D TOPO Cloning Kits utilize a highly efficient 5 minute cloning strategy TOPO Cloning to directionally clone a blunt end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System Blunt end PCR products clone directionally into the pENTR SD D TOPO entry vector at greater than 90 efficiency and include an upstream Shine Dalgarno sequence for expression in prokaryotes The kit comes with everything necessary to clone and select your PCR amplified gene of interest • Gateway System Ready Rapidly shuttle cloned genes between multiple vector systems• Fast and Easy Go from PCR to Gateway Entry clone in just 3 steps and as little as 5 minutes hands on time• Efficient Achieve over 90 clones with correct insert in the right direction• Proven Reliable performance for over a decade pENTR SD D TOPO Cloning Kits Overview VECTOR pENTR SD D TOPO Vector Directional cloning vector for entry to the Gateway System and optimized for prokaryotic expression CLONING METHOD Directional TOPO Cloning Topoisomerase I based 5 minute directional ligation of blunt end proofreading polymerase amplified PCR products to the vector COMPETENT CELLS Two Options Choose from kits with either high efficiency or fast growing competent cells Simple Access to the Gateway SystemFor access to the Gateway System just PCR amplify your gene of interest and add the product straight to the provided topoisomerase charged pENTR SD D TOPO vector incubate 5 minutes and transform the provided competent E coli cells The resulting attL containing Gateway Entry clones are ready for efficient recombination with your choice of Gateway Destination vectors Optimized pENTR SD D TOPO VectorThe pENTR SD D TOPO vector Figure 1 includes a T7 gene 10 translational enhancer and a ribosome binding site RBS for optimal expression of native protein after recombination with a prokaryotic Gateway destination vectors You can also use the vector for expression in other hosts systems by recombination with different destination vectors The vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site This allows clones to be easily sequence verified and recombined into your choice of attR containing Gateway destination vectors A Kanamycin resistance gene and a pUC origin are used for selection and high copy propagation in E coli Simplified Directional CloningWith Directional TOPO cloning technology there is no need for PCR clean up vector preparation or other time intensive DNA manipulation steps Just add your PCR reaction straight to the provided topoisomerase charged vector incubate 5 minutes transform and obtain up to 90 directionally inserted clones A four base overhang on the vector pairs with a four base sequence designed into the forward primer used in your PCR reaction to provide directionality to the topoisomerase ligation reaction Figure 2 The Power of Gateway Recombination Cloning TechnologyGateway recombination cloning technology circumvents the limitations of restriction mediated cloning enabling you to access virtually any expression system in a simple one hour 99 efficient and reversible Gateway recombination reaction The ability to move the same sequence of DNA between different vectors without using restriction enzymes ligase subcloning steps screening of countless colonies or re sequencing will help save you time money and effort Leading Cloning TechnologiesWhen it comes to cloning TOPO cloning technology and Gateway recombination cloning technology have been a reliable partner for thousands of scientists for over ten years Fast simple to use and efficient TOPO cloning and Gateway recombination allow for rapid cloning and subsequent transferring of genes between a wide assortment of Gateway expression vectors Kit OptionsThe pENTR SD D TOPO Cloning Kit can be purchased with either TOP10 competent cells for standard cloning or Mach1 T1R competent cells for fast growth
    https://www.bioz.com/result/pentr sd d topo vector/product/Thermo Fisher
    Average 99 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    pentr sd d topo vector - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Stanniocalcin-1 Reduces Tumor Size in Human Hepatocellular Carcinoma
    Article Snippet: .. Construction of pLenti6.3/TO/V5-DEST-STC1 plasmid Human STC1 cDNA encoding the wild-type full-length protein without the stop codon was amplified by PCR and cloned into pENTR/SD/D-TOPO (Life Technologies) according to the manufacturer’s instruction. .. The STC1 insert was then cut from pENTR/SD/D-TOPO and cloned into the expression vector pLenti6.3/TO/V5/-DEST (Life Technologies) using Gateway LR Clonase II Plus Enzyme Mix (Life Technologies).

    Article Title: LZTR1 facilitates polyubiquitination and degradation of RAS-GTPases
    Article Snippet: .. Epitope tags (Flag, Myc, and HA) and single base substitutions were inserted and mutated using specific primers and the KOD-Plus-Mutagenesis Kit (Toyobo), respectively (Supplementary Table ). pENTR-SD-D-TOPO constructs were subcloned into the pcDNA3.2/V5-DEST and pLenti6.3/TO/V5-DEST vectors (Thermo Fisher Scientific) using the Gateway cloning system. .. HEK293 cells were transfected with 10 nM ON-TARGETplus Non-Targeting Pool (control-siRNA) or 10 nM ON-TARGETplus SMART Pool-human LZTR1 (LZTR1 siRNA) (GE Healthcare Dharmacon, Lafayette, CO) using Lipofectamine RNAiMAX (Thermo Fisher Scientific) following the manufacturer’s protocol.

    Article Title: Jasmonates act positively in adventitious root formation in petunia cuttings
    Article Snippet: .. Generation of PhAOC-RNAi plants A fragment of 180 bp was amplified from the PhAOC cDNA (GenBank: EU652410) using Proofstart DNA-Polymerase and the primers listed in Additional file : Table S3, cloned into pENTR/SD/D-TOPO -Vector (Gateway® Cloning, Invitrogen), and transferred into the RNAi vector pHellsgate 8 [ ]. .. Additionally the ccdB gene of pHellsgate 8 was cut out to receive the empty vector control plasmid without the RNAi cassette (pHell).

    Article Title: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis
    Article Snippet: .. To generate Pro LBD16 :LBD16:4xMyc in the lbd16 mutant background, the promoter region of LBD16 , encompassing − 1309 to − 21 bp relative to the AUG codon, was amplified by PCR using the pfu DNA polymerase with primers harboring the Not I (N-terminus) and Asc I (C-terminus) sites, and was cloned into the pENTR™/SD/D-TOPO (Invitrogen) vector to yield pENTR™/SD/D-TOPO:Pro LBD16 . .. The LBD16 DNA fragment was inserted into the pENTR™/SD/D-TOPO:Pro LBD16 plasmid with the Asc I restriction site in both the N- and C-terminus to yield a pENTR™/SD/D-TOPO:Pro LBD16 :LBD16 plasmid.

    Article Title: Physical and thermodynamic characterization of the rice gibberellin receptor/gibberellin/DELLA protein complex
    Article Snippet: .. For each truncated SLR1, PCR-amplified cDNA was inserted into a pENTR/SD/D-TOPO vector using pENTR/SD/D-TOPO Cloning Kit reagents (Invitrogen, Carlsbad, CA), and expression plasmids were then constructed using LR Reaction kit reagents (Invitrogen, Carlsbad, CA). .. Each SLR1 cDNA was cloned into a pDEST-trx expression vector to create the DNA for the corresponding thioredoxin–hexahistidine (Trx–(His)6 -) SLR1 fusion protein.

    Article Title: Identification of methionine sulfoxide diastereomers in immunoglobulin gamma antibodies using methionine sulfoxide reductase enzymes
    Article Snippet: .. The amplified DNA fragments were cloned into pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA) to contain a V5-His tag sequence (KGG RAD PAF LYK VVI NSK LEG KPI PNP LLG LDS TRT GHH HHH H) engineered at the C-terminus of the expressed proteins and transformed into Escherichia coli strain TOP10 following the manufacturer instruction manual. .. Positive clones were screened and all DNA sequences were verified by DNA sequencing (Amplicon Express, Pullman, WA).

    Amplification:

    Article Title: Stanniocalcin-1 Reduces Tumor Size in Human Hepatocellular Carcinoma
    Article Snippet: .. Construction of pLenti6.3/TO/V5-DEST-STC1 plasmid Human STC1 cDNA encoding the wild-type full-length protein without the stop codon was amplified by PCR and cloned into pENTR/SD/D-TOPO (Life Technologies) according to the manufacturer’s instruction. .. The STC1 insert was then cut from pENTR/SD/D-TOPO and cloned into the expression vector pLenti6.3/TO/V5/-DEST (Life Technologies) using Gateway LR Clonase II Plus Enzyme Mix (Life Technologies).

    Article Title: Jasmonates act positively in adventitious root formation in petunia cuttings
    Article Snippet: .. Generation of PhAOC-RNAi plants A fragment of 180 bp was amplified from the PhAOC cDNA (GenBank: EU652410) using Proofstart DNA-Polymerase and the primers listed in Additional file : Table S3, cloned into pENTR/SD/D-TOPO -Vector (Gateway® Cloning, Invitrogen), and transferred into the RNAi vector pHellsgate 8 [ ]. .. Additionally the ccdB gene of pHellsgate 8 was cut out to receive the empty vector control plasmid without the RNAi cassette (pHell).

    Article Title: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis
    Article Snippet: .. To generate Pro LBD16 :LBD16:4xMyc in the lbd16 mutant background, the promoter region of LBD16 , encompassing − 1309 to − 21 bp relative to the AUG codon, was amplified by PCR using the pfu DNA polymerase with primers harboring the Not I (N-terminus) and Asc I (C-terminus) sites, and was cloned into the pENTR™/SD/D-TOPO (Invitrogen) vector to yield pENTR™/SD/D-TOPO:Pro LBD16 . .. The LBD16 DNA fragment was inserted into the pENTR™/SD/D-TOPO:Pro LBD16 plasmid with the Asc I restriction site in both the N- and C-terminus to yield a pENTR™/SD/D-TOPO:Pro LBD16 :LBD16 plasmid.

    Article Title: Identification of methionine sulfoxide diastereomers in immunoglobulin gamma antibodies using methionine sulfoxide reductase enzymes
    Article Snippet: .. The amplified DNA fragments were cloned into pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA) to contain a V5-His tag sequence (KGG RAD PAF LYK VVI NSK LEG KPI PNP LLG LDS TRT GHH HHH H) engineered at the C-terminus of the expressed proteins and transformed into Escherichia coli strain TOP10 following the manufacturer instruction manual. .. Positive clones were screened and all DNA sequences were verified by DNA sequencing (Amplicon Express, Pullman, WA).

    Mutagenesis:

    Article Title: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis
    Article Snippet: .. To generate Pro LBD16 :LBD16:4xMyc in the lbd16 mutant background, the promoter region of LBD16 , encompassing − 1309 to − 21 bp relative to the AUG codon, was amplified by PCR using the pfu DNA polymerase with primers harboring the Not I (N-terminus) and Asc I (C-terminus) sites, and was cloned into the pENTR™/SD/D-TOPO (Invitrogen) vector to yield pENTR™/SD/D-TOPO:Pro LBD16 . .. The LBD16 DNA fragment was inserted into the pENTR™/SD/D-TOPO:Pro LBD16 plasmid with the Asc I restriction site in both the N- and C-terminus to yield a pENTR™/SD/D-TOPO:Pro LBD16 :LBD16 plasmid.

    Construct:

    Article Title: LZTR1 facilitates polyubiquitination and degradation of RAS-GTPases
    Article Snippet: .. Epitope tags (Flag, Myc, and HA) and single base substitutions were inserted and mutated using specific primers and the KOD-Plus-Mutagenesis Kit (Toyobo), respectively (Supplementary Table ). pENTR-SD-D-TOPO constructs were subcloned into the pcDNA3.2/V5-DEST and pLenti6.3/TO/V5-DEST vectors (Thermo Fisher Scientific) using the Gateway cloning system. .. HEK293 cells were transfected with 10 nM ON-TARGETplus Non-Targeting Pool (control-siRNA) or 10 nM ON-TARGETplus SMART Pool-human LZTR1 (LZTR1 siRNA) (GE Healthcare Dharmacon, Lafayette, CO) using Lipofectamine RNAiMAX (Thermo Fisher Scientific) following the manufacturer’s protocol.

    Article Title: Physical and thermodynamic characterization of the rice gibberellin receptor/gibberellin/DELLA protein complex
    Article Snippet: .. For each truncated SLR1, PCR-amplified cDNA was inserted into a pENTR/SD/D-TOPO vector using pENTR/SD/D-TOPO Cloning Kit reagents (Invitrogen, Carlsbad, CA), and expression plasmids were then constructed using LR Reaction kit reagents (Invitrogen, Carlsbad, CA). .. Each SLR1 cDNA was cloned into a pDEST-trx expression vector to create the DNA for the corresponding thioredoxin–hexahistidine (Trx–(His)6 -) SLR1 fusion protein.

    Polymerase Chain Reaction:

    Article Title: Stanniocalcin-1 Reduces Tumor Size in Human Hepatocellular Carcinoma
    Article Snippet: .. Construction of pLenti6.3/TO/V5-DEST-STC1 plasmid Human STC1 cDNA encoding the wild-type full-length protein without the stop codon was amplified by PCR and cloned into pENTR/SD/D-TOPO (Life Technologies) according to the manufacturer’s instruction. .. The STC1 insert was then cut from pENTR/SD/D-TOPO and cloned into the expression vector pLenti6.3/TO/V5/-DEST (Life Technologies) using Gateway LR Clonase II Plus Enzyme Mix (Life Technologies).

    Article Title: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis
    Article Snippet: .. To generate Pro LBD16 :LBD16:4xMyc in the lbd16 mutant background, the promoter region of LBD16 , encompassing − 1309 to − 21 bp relative to the AUG codon, was amplified by PCR using the pfu DNA polymerase with primers harboring the Not I (N-terminus) and Asc I (C-terminus) sites, and was cloned into the pENTR™/SD/D-TOPO (Invitrogen) vector to yield pENTR™/SD/D-TOPO:Pro LBD16 . .. The LBD16 DNA fragment was inserted into the pENTR™/SD/D-TOPO:Pro LBD16 plasmid with the Asc I restriction site in both the N- and C-terminus to yield a pENTR™/SD/D-TOPO:Pro LBD16 :LBD16 plasmid.

    Article Title: Physical and thermodynamic characterization of the rice gibberellin receptor/gibberellin/DELLA protein complex
    Article Snippet: .. For each truncated SLR1, PCR-amplified cDNA was inserted into a pENTR/SD/D-TOPO vector using pENTR/SD/D-TOPO Cloning Kit reagents (Invitrogen, Carlsbad, CA), and expression plasmids were then constructed using LR Reaction kit reagents (Invitrogen, Carlsbad, CA). .. Each SLR1 cDNA was cloned into a pDEST-trx expression vector to create the DNA for the corresponding thioredoxin–hexahistidine (Trx–(His)6 -) SLR1 fusion protein.

    Generated:

    Article Title: A Tonoplast-Associated Calcium-Signaling Module Dampens ABA Signaling during Stomatal Movement
    Article Snippet: .. The entry vectors containing the gene-specific sequences were generated in pENTR/SD/D-TOPO (Invitrogen) with the following primer pairs: ZP195/ZP416 for PAT10 , ZP1845/ZP1846 for INT1 , ZP983/ZP3479 for CIPK9 , ZP3482/ZP3483 for CIPK17 , and ZP628/ZP629 for NHX2 . .. The CA mutations of the CIPKs were generated using a Phusion site-directed mutagenesis kit according to the manufacturer’s (ThermoFisher) instructions with the following primers: ZP3874/ZP3875 for CIPK9CA and ZP3856/ZP3857 for CIPK17CA .

    Expressing:

    Article Title: Physical and thermodynamic characterization of the rice gibberellin receptor/gibberellin/DELLA protein complex
    Article Snippet: .. For each truncated SLR1, PCR-amplified cDNA was inserted into a pENTR/SD/D-TOPO vector using pENTR/SD/D-TOPO Cloning Kit reagents (Invitrogen, Carlsbad, CA), and expression plasmids were then constructed using LR Reaction kit reagents (Invitrogen, Carlsbad, CA). .. Each SLR1 cDNA was cloned into a pDEST-trx expression vector to create the DNA for the corresponding thioredoxin–hexahistidine (Trx–(His)6 -) SLR1 fusion protein.

    Sequencing:

    Article Title: Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase
    Article Snippet: .. Primers were designed to clone the entire sequence into the pENTR-SD/D-TOPO vector (Invitrogen). .. This construct was altered by PCR-mediated mutagenesis using primers dhhs_5′_Mut, 3′-GCAAAATTTGATCATCATTCCCCATGGGTGGGTAACTG-5′, and dhhs_3′_Mut, 3′-CAGTTACCCACCCATGGGGAATGATGATCAAATTTT GC-5′ to create the DHHS active site mutant expression construct.

    Article Title: Identification of methionine sulfoxide diastereomers in immunoglobulin gamma antibodies using methionine sulfoxide reductase enzymes
    Article Snippet: .. The amplified DNA fragments were cloned into pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA) to contain a V5-His tag sequence (KGG RAD PAF LYK VVI NSK LEG KPI PNP LLG LDS TRT GHH HHH H) engineered at the C-terminus of the expressed proteins and transformed into Escherichia coli strain TOP10 following the manufacturer instruction manual. .. Positive clones were screened and all DNA sequences were verified by DNA sequencing (Amplicon Express, Pullman, WA).

    Transformation Assay:

    Article Title: Identification of methionine sulfoxide diastereomers in immunoglobulin gamma antibodies using methionine sulfoxide reductase enzymes
    Article Snippet: .. The amplified DNA fragments were cloned into pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA) to contain a V5-His tag sequence (KGG RAD PAF LYK VVI NSK LEG KPI PNP LLG LDS TRT GHH HHH H) engineered at the C-terminus of the expressed proteins and transformed into Escherichia coli strain TOP10 following the manufacturer instruction manual. .. Positive clones were screened and all DNA sequences were verified by DNA sequencing (Amplicon Express, Pullman, WA).

    Plasmid Preparation:

    Article Title: Stanniocalcin-1 Reduces Tumor Size in Human Hepatocellular Carcinoma
    Article Snippet: .. Construction of pLenti6.3/TO/V5-DEST-STC1 plasmid Human STC1 cDNA encoding the wild-type full-length protein without the stop codon was amplified by PCR and cloned into pENTR/SD/D-TOPO (Life Technologies) according to the manufacturer’s instruction. .. The STC1 insert was then cut from pENTR/SD/D-TOPO and cloned into the expression vector pLenti6.3/TO/V5/-DEST (Life Technologies) using Gateway LR Clonase II Plus Enzyme Mix (Life Technologies).

    Article Title: Jasmonates act positively in adventitious root formation in petunia cuttings
    Article Snippet: .. Generation of PhAOC-RNAi plants A fragment of 180 bp was amplified from the PhAOC cDNA (GenBank: EU652410) using Proofstart DNA-Polymerase and the primers listed in Additional file : Table S3, cloned into pENTR/SD/D-TOPO -Vector (Gateway® Cloning, Invitrogen), and transferred into the RNAi vector pHellsgate 8 [ ]. .. Additionally the ccdB gene of pHellsgate 8 was cut out to receive the empty vector control plasmid without the RNAi cassette (pHell).

    Article Title: LBD16 and LBD18 acting downstream of ARF7 and ARF19 are involved in adventitious root formation in Arabidopsis
    Article Snippet: .. To generate Pro LBD16 :LBD16:4xMyc in the lbd16 mutant background, the promoter region of LBD16 , encompassing − 1309 to − 21 bp relative to the AUG codon, was amplified by PCR using the pfu DNA polymerase with primers harboring the Not I (N-terminus) and Asc I (C-terminus) sites, and was cloned into the pENTR™/SD/D-TOPO (Invitrogen) vector to yield pENTR™/SD/D-TOPO:Pro LBD16 . .. The LBD16 DNA fragment was inserted into the pENTR™/SD/D-TOPO:Pro LBD16 plasmid with the Asc I restriction site in both the N- and C-terminus to yield a pENTR™/SD/D-TOPO:Pro LBD16 :LBD16 plasmid.

    Article Title: Physical and thermodynamic characterization of the rice gibberellin receptor/gibberellin/DELLA protein complex
    Article Snippet: .. For each truncated SLR1, PCR-amplified cDNA was inserted into a pENTR/SD/D-TOPO vector using pENTR/SD/D-TOPO Cloning Kit reagents (Invitrogen, Carlsbad, CA), and expression plasmids were then constructed using LR Reaction kit reagents (Invitrogen, Carlsbad, CA). .. Each SLR1 cDNA was cloned into a pDEST-trx expression vector to create the DNA for the corresponding thioredoxin–hexahistidine (Trx–(His)6 -) SLR1 fusion protein.

    Article Title: Huntingtin interacting protein 14 is an oncogenic human protein: palmitoyl acyltransferase
    Article Snippet: .. Primers were designed to clone the entire sequence into the pENTR-SD/D-TOPO vector (Invitrogen). .. This construct was altered by PCR-mediated mutagenesis using primers dhhs_5′_Mut, 3′-GCAAAATTTGATCATCATTCCCCATGGGTGGGTAACTG-5′, and dhhs_3′_Mut, 3′-CAGTTACCCACCCATGGGGAATGATGATCAAATTTT GC-5′ to create the DHHS active site mutant expression construct.

    Article Title: Identification of methionine sulfoxide diastereomers in immunoglobulin gamma antibodies using methionine sulfoxide reductase enzymes
    Article Snippet: .. The amplified DNA fragments were cloned into pENTR/SD/D-TOPO vector (Invitrogen, Carlsbad, CA) to contain a V5-His tag sequence (KGG RAD PAF LYK VVI NSK LEG KPI PNP LLG LDS TRT GHH HHH H) engineered at the C-terminus of the expressed proteins and transformed into Escherichia coli strain TOP10 following the manufacturer instruction manual. .. Positive clones were screened and all DNA sequences were verified by DNA sequencing (Amplicon Express, Pullman, WA).

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  • 99
    Thermo Fisher pentr sd d topo cloning kit
    Pentr Sd D Topo Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr sd d topo cloning kit/product/Thermo Fisher
    Average 99 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    pentr sd d topo cloning kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

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