pentr sd d topo vector (Thermo Fisher)
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Name:
pENTR SD D TOPO Cloning Kit
Description:
The pENTR SD D TOPO Cloning Kits utilize a highly efficient 5 minute cloning strategy TOPO Cloning to directionally clone a blunt end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System Blunt end PCR products clone directionally into the pENTR SD D TOPO entry vector at greater than 90 efficiency and include an upstream Shine Dalgarno sequence for expression in prokaryotes The kit comes with everything necessary to clone and select your PCR amplified gene of interest • Gateway System Ready Rapidly shuttle cloned genes between multiple vector systems• Fast and Easy Go from PCR to Gateway Entry clone in just 3 steps and as little as 5 minutes hands on time• Efficient Achieve over 90 clones with correct insert in the right direction• Proven Reliable performance for over a decade pENTR SD D TOPO Cloning Kits Overview VECTOR pENTR SD D TOPO Vector Directional cloning vector for entry to the Gateway System and optimized for prokaryotic expression CLONING METHOD Directional TOPO Cloning Topoisomerase I based 5 minute directional ligation of blunt end proofreading polymerase amplified PCR products to the vector COMPETENT CELLS Two Options Choose from kits with either high efficiency or fast growing competent cells Simple Access to the Gateway SystemFor access to the Gateway System just PCR amplify your gene of interest and add the product straight to the provided topoisomerase charged pENTR SD D TOPO vector incubate 5 minutes and transform the provided competent E coli cells The resulting attL containing Gateway Entry clones are ready for efficient recombination with your choice of Gateway Destination vectors Optimized pENTR SD D TOPO VectorThe pENTR SD D TOPO vector Figure 1 includes a T7 gene 10 translational enhancer and a ribosome binding site RBS for optimal expression of native protein after recombination with a prokaryotic Gateway destination vectors You can also use the vector for expression in other hosts systems by recombination with different destination vectors The vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site This allows clones to be easily sequence verified and recombined into your choice of attR containing Gateway destination vectors A Kanamycin resistance gene and a pUC origin are used for selection and high copy propagation in E coli Simplified Directional CloningWith Directional TOPO cloning technology there is no need for PCR clean up vector preparation or other time intensive DNA manipulation steps Just add your PCR reaction straight to the provided topoisomerase charged vector incubate 5 minutes transform and obtain up to 90 directionally inserted clones A four base overhang on the vector pairs with a four base sequence designed into the forward primer used in your PCR reaction to provide directionality to the topoisomerase ligation reaction Figure 2 The Power of Gateway Recombination Cloning TechnologyGateway recombination cloning technology circumvents the limitations of restriction mediated cloning enabling you to access virtually any expression system in a simple one hour 99 efficient and reversible Gateway recombination reaction The ability to move the same sequence of DNA between different vectors without using restriction enzymes ligase subcloning steps screening of countless colonies or re sequencing will help save you time money and effort Leading Cloning TechnologiesWhen it comes to cloning TOPO cloning technology and Gateway recombination cloning technology have been a reliable partner for thousands of scientists for over ten years Fast simple to use and efficient TOPO cloning and Gateway recombination allow for rapid cloning and subsequent transferring of genes between a wide assortment of Gateway expression vectors Kit OptionsThe pENTR SD D TOPO Cloning Kit can be purchased with either TOP10 competent cells for standard cloning or Mach1 T1R competent cells for fast growth
Catalog Number:
k242020
Price:
None
Applications:
Cloning|Entry Vectors & Kits|Gateway Cloning|pENTR Vectors & Kits
Category:
DNA Vectors Clones Purified Nucleic Acids Libraries
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Structured Review
Thermo Fisher
pentr sd d topo vector
The pENTR SD D TOPO Cloning Kits utilize a highly efficient 5 minute cloning strategy TOPO Cloning to directionally clone a blunt end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System Blunt end PCR products clone directionally into the pENTR SD D TOPO entry vector at greater than 90 efficiency and include an upstream Shine Dalgarno sequence for expression in prokaryotes The kit comes with everything necessary to clone and select your PCR amplified gene of interest • Gateway System Ready Rapidly shuttle cloned genes between multiple vector systems• Fast and Easy Go from PCR to Gateway Entry clone in just 3 steps and as little as 5 minutes hands on time• Efficient Achieve over 90 clones with correct insert in the right direction• Proven Reliable performance for over a decade pENTR SD D TOPO Cloning Kits Overview VECTOR pENTR SD D TOPO Vector Directional cloning vector for entry to the Gateway System and optimized for prokaryotic expression CLONING METHOD Directional TOPO Cloning Topoisomerase I based 5 minute directional ligation of blunt end proofreading polymerase amplified PCR products to the vector COMPETENT CELLS Two Options Choose from kits with either high efficiency or fast growing competent cells Simple Access to the Gateway SystemFor access to the Gateway System just PCR amplify your gene of interest and add the product straight to the provided topoisomerase charged pENTR SD D TOPO vector incubate 5 minutes and transform the provided competent E coli cells The resulting attL containing Gateway Entry clones are ready for efficient recombination with your choice of Gateway Destination vectors Optimized pENTR SD D TOPO VectorThe pENTR SD D TOPO vector Figure 1 includes a T7 gene 10 translational enhancer and a ribosome binding site RBS for optimal expression of native protein after recombination with a prokaryotic Gateway destination vectors You can also use the vector for expression in other hosts systems by recombination with different destination vectors The vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site This allows clones to be easily sequence verified and recombined into your choice of attR containing Gateway destination vectors A Kanamycin resistance gene and a pUC origin are used for selection and high copy propagation in E coli Simplified Directional CloningWith Directional TOPO cloning technology there is no need for PCR clean up vector preparation or other time intensive DNA manipulation steps Just add your PCR reaction straight to the provided topoisomerase charged vector incubate 5 minutes transform and obtain up to 90 directionally inserted clones A four base overhang on the vector pairs with a four base sequence designed into the forward primer used in your PCR reaction to provide directionality to the topoisomerase ligation reaction Figure 2 The Power of Gateway Recombination Cloning TechnologyGateway recombination cloning technology circumvents the limitations of restriction mediated cloning enabling you to access virtually any expression system in a simple one hour 99 efficient and reversible Gateway recombination reaction The ability to move the same sequence of DNA between different vectors without using restriction enzymes ligase subcloning steps screening of countless colonies or re sequencing will help save you time money and effort Leading Cloning TechnologiesWhen it comes to cloning TOPO cloning technology and Gateway recombination cloning technology have been a reliable partner for thousands of scientists for over ten years Fast simple to use and efficient TOPO cloning and Gateway recombination allow for rapid cloning and subsequent transferring of genes between a wide assortment of Gateway expression vectors Kit OptionsThe pENTR SD D TOPO Cloning Kit can be purchased with either TOP10 competent cells for standard cloning or Mach1 T1R competent cells for fast growth
https://www.bioz.com/result/pentr sd d topo vector/product/Thermo Fisher
Average 99 stars, based on 69 article reviews
Price from $9.99 to $1999.99
The pENTR SD D TOPO Cloning Kits utilize a highly efficient 5 minute cloning strategy TOPO Cloning to directionally clone a blunt end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System Blunt end PCR products clone directionally into the pENTR SD D TOPO entry vector at greater than 90 efficiency and include an upstream Shine Dalgarno sequence for expression in prokaryotes The kit comes with everything necessary to clone and select your PCR amplified gene of interest • Gateway System Ready Rapidly shuttle cloned genes between multiple vector systems• Fast and Easy Go from PCR to Gateway Entry clone in just 3 steps and as little as 5 minutes hands on time• Efficient Achieve over 90 clones with correct insert in the right direction• Proven Reliable performance for over a decade pENTR SD D TOPO Cloning Kits Overview VECTOR pENTR SD D TOPO Vector Directional cloning vector for entry to the Gateway System and optimized for prokaryotic expression CLONING METHOD Directional TOPO Cloning Topoisomerase I based 5 minute directional ligation of blunt end proofreading polymerase amplified PCR products to the vector COMPETENT CELLS Two Options Choose from kits with either high efficiency or fast growing competent cells Simple Access to the Gateway SystemFor access to the Gateway System just PCR amplify your gene of interest and add the product straight to the provided topoisomerase charged pENTR SD D TOPO vector incubate 5 minutes and transform the provided competent E coli cells The resulting attL containing Gateway Entry clones are ready for efficient recombination with your choice of Gateway Destination vectors Optimized pENTR SD D TOPO VectorThe pENTR SD D TOPO vector Figure 1 includes a T7 gene 10 translational enhancer and a ribosome binding site RBS for optimal expression of native protein after recombination with a prokaryotic Gateway destination vectors You can also use the vector for expression in other hosts systems by recombination with different destination vectors The vector has M13 and T7 primer sequencing sites and attL recombination sites flanking the PCR product insertion site This allows clones to be easily sequence verified and recombined into your choice of attR containing Gateway destination vectors A Kanamycin resistance gene and a pUC origin are used for selection and high copy propagation in E coli Simplified Directional CloningWith Directional TOPO cloning technology there is no need for PCR clean up vector preparation or other time intensive DNA manipulation steps Just add your PCR reaction straight to the provided topoisomerase charged vector incubate 5 minutes transform and obtain up to 90 directionally inserted clones A four base overhang on the vector pairs with a four base sequence designed into the forward primer used in your PCR reaction to provide directionality to the topoisomerase ligation reaction Figure 2 The Power of Gateway Recombination Cloning TechnologyGateway recombination cloning technology circumvents the limitations of restriction mediated cloning enabling you to access virtually any expression system in a simple one hour 99 efficient and reversible Gateway recombination reaction The ability to move the same sequence of DNA between different vectors without using restriction enzymes ligase subcloning steps screening of countless colonies or re sequencing will help save you time money and effort Leading Cloning TechnologiesWhen it comes to cloning TOPO cloning technology and Gateway recombination cloning technology have been a reliable partner for thousands of scientists for over ten years Fast simple to use and efficient TOPO cloning and Gateway recombination allow for rapid cloning and subsequent transferring of genes between a wide assortment of Gateway expression vectors Kit OptionsThe pENTR SD D TOPO Cloning Kit can be purchased with either TOP10 competent cells for standard cloning or Mach1 T1R competent cells for fast growth
https://www.bioz.com/result/pentr sd d topo vector/product/Thermo Fisher
Average 99 stars, based on 69 article reviews
Price from $9.99 to $1999.99
pentr sd d topo vector - by Bioz Stars,
2021-01
99/100 stars
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