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Thermo Fisher pentr d
Pentr D, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentr d/product/Thermo Fisher
Average 99 stars, based on 38 article reviews
Price from $9.99 to $1999.99
pentr d - by Bioz Stars, 2020-09
99/100 stars

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Clone Assay:

Article Title: BRANCHLESS TRICHOMES links cell shape and cell cycle control in Arabidopsis trichomes
Article Snippet: .. The full-length BLT coding region was PCR amplified from the BAC clone FIN19 using primers ENSIF (5′-CACCATGAAGGATATGAAGATGCAGAGC-3′) and ENS1R1 (5′-TCAAGGAGGAGGAGAGGAGAGA-3′), which contains a stop codon, or ENS1R2 (5′-AGGAGGAGGAGAGGAGAGAAGAG-3′), which lacks a stop codon, and cloned into the Gateway vector pENTR following the manufacturer's protocol (Invitrogen). .. Error-free entry clones were confirmed by DNA sequencing before attL recombination into the Gateway-compatible destination binary vector pLEELA-pGL2 ( ) for overexpression in developing trichomes.

Article Title: Sfr1, a Tetrahymena thermophila Sfi1 Repeat Protein, Modulates the Production of Cortical Row Basal Bodies
Article Snippet: .. The exogenous N-terminal GFP fusion used to localize Sfr1 was generated by cloning SFR1 first into the pENTR4 Dual Selection Vector (Invitrogen) and then subcloning it into pBS-MTT1 -GFP-gtw ( ) by utilizing the Gateway cloning system (Invitrogen). ..

Article Title: Basal Body Components Exhibit Differential Protein Dynamics during Nascent Basal Body Assembly
Article Snippet: .. Plasmids were constructed by cloning the coding sequence of each gene into the pENTR-D Gateway Entry Vector (Invitrogen, Carlsbad, CA). .. The coding sequence was then Gateway cloned into pBSmttGFPgtw or pIGFgtw ( ). pBSmttGFPgtw contains the inducible GFP-tagging cassette from pIGFgtw cloned upstream of a cycloheximide-resistance allele of the rpL29 gene, which allows the tagged construct to integrate into the endogenous rpL29 locus (Chalker, Washington University).

Article Title: Regulation of Cell Proliferation in the Stomatal Lineage by the Arabidopsis MYB FOUR LIPS via Direct Targeting of Core Cell Cycle Genes [W]
Article Snippet: .. ProCDC6A:GUS staining was detected as by except that leaves were cleared for 2 to 7 d. To make Pro35S:E2Fa-GFP transgenic lines, E2Fa (At2g36010) cDNA was cloned into a pENTR Gateway vector (Invitrogen), mobilized into a pGWB5 binary destination vector, and then Agrobacterium tumefaciens was transformed into plants using a floral dip method ( ). .. The ProCDKB1;1:GFP and ProCDKB1;2:GFP transcriptional fusions were generated via PCR amplification of ~0.5 and 2.9 kb, respectively, of sequence upstream of the start codon (primers shown in Supplemental Table 2 online).

Article Title: A New XRCC1-Containing Complex and Its Role in Cellular Survival of Methyl Methanesulfonate Treatment
Article Snippet: .. Full-length cDNAs encoding APTX and PNK were amplified from the first-strand cDNA generated by RT-PCR using RNA isolated from HeLa cells and cloned into the Gateway PENTR TOPO vector (Invitrogen). ..

Article Title: NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila
Article Snippet: .. The UAS-NFAT[DN] transgene was generated by cloning the Rel-Homology Domain (RHD) of the NFAT gene into the pENTR-D Gateway cloning vector (Invitrogen Inc.) followed by insertion into the pTW Gateway compatible destination vector. .. Larval dissection, staining and confocal microscopy were performed according to standard protocols ( ).

Amplification:

Article Title: BRANCHLESS TRICHOMES links cell shape and cell cycle control in Arabidopsis trichomes
Article Snippet: .. The full-length BLT coding region was PCR amplified from the BAC clone FIN19 using primers ENSIF (5′-CACCATGAAGGATATGAAGATGCAGAGC-3′) and ENS1R1 (5′-TCAAGGAGGAGGAGAGGAGAGA-3′), which contains a stop codon, or ENS1R2 (5′-AGGAGGAGGAGAGGAGAGAAGAG-3′), which lacks a stop codon, and cloned into the Gateway vector pENTR following the manufacturer's protocol (Invitrogen). .. Error-free entry clones were confirmed by DNA sequencing before attL recombination into the Gateway-compatible destination binary vector pLEELA-pGL2 ( ) for overexpression in developing trichomes.

Article Title: A New XRCC1-Containing Complex and Its Role in Cellular Survival of Methyl Methanesulfonate Treatment
Article Snippet: .. Full-length cDNAs encoding APTX and PNK were amplified from the first-strand cDNA generated by RT-PCR using RNA isolated from HeLa cells and cloned into the Gateway PENTR TOPO vector (Invitrogen). ..

Polymerase Chain Reaction:

Article Title: BRANCHLESS TRICHOMES links cell shape and cell cycle control in Arabidopsis trichomes
Article Snippet: .. The full-length BLT coding region was PCR amplified from the BAC clone FIN19 using primers ENSIF (5′-CACCATGAAGGATATGAAGATGCAGAGC-3′) and ENS1R1 (5′-TCAAGGAGGAGGAGAGGAGAGA-3′), which contains a stop codon, or ENS1R2 (5′-AGGAGGAGGAGAGGAGAGAAGAG-3′), which lacks a stop codon, and cloned into the Gateway vector pENTR following the manufacturer's protocol (Invitrogen). .. Error-free entry clones were confirmed by DNA sequencing before attL recombination into the Gateway-compatible destination binary vector pLEELA-pGL2 ( ) for overexpression in developing trichomes.

Isolation:

Article Title: A New XRCC1-Containing Complex and Its Role in Cellular Survival of Methyl Methanesulfonate Treatment
Article Snippet: .. Full-length cDNAs encoding APTX and PNK were amplified from the first-strand cDNA generated by RT-PCR using RNA isolated from HeLa cells and cloned into the Gateway PENTR TOPO vector (Invitrogen). ..

Subcloning:

Article Title: Sfr1, a Tetrahymena thermophila Sfi1 Repeat Protein, Modulates the Production of Cortical Row Basal Bodies
Article Snippet: .. The exogenous N-terminal GFP fusion used to localize Sfr1 was generated by cloning SFR1 first into the pENTR4 Dual Selection Vector (Invitrogen) and then subcloning it into pBS-MTT1 -GFP-gtw ( ) by utilizing the Gateway cloning system (Invitrogen). ..

Article Title: Wnt/β-catenin Signaling Pathway Regulates Specific lncRNAs That Impact Dermal Fibroblasts and Skin Fibrosis
Article Snippet: .. Wincr1 lentivirus was generated by subcloning Wincr1 from pMA-T into the Nco1-EcoRV region of pENTR4 (Thermo Fisher Cat. No. A10465) vector and then swapping it into the pGK destination vector (Addgene Cat. No. 19068). .. Lentivirus was generated in 293T (f-variant) cells (ATCC Cat. No. 3216) by simultaneously transfecting pGK Destination Vector containing Wincr1 (4.5 μg), PMD2G helper plasmid (1.6 μg) (Addgene Cat. No. 12259), and pCMV-dR874 plasmid (3.2 μg), using Lipofectamine 3000 reagent (18 μL) (Invitrogen Cat. No. L30000015) in 1.5 mL Opti-MEM (Thermo Fisher Cat. No. 31985062) media in a 6 cm dish.

Construct:

Article Title: Basal Body Components Exhibit Differential Protein Dynamics during Nascent Basal Body Assembly
Article Snippet: .. Plasmids were constructed by cloning the coding sequence of each gene into the pENTR-D Gateway Entry Vector (Invitrogen, Carlsbad, CA). .. The coding sequence was then Gateway cloned into pBSmttGFPgtw or pIGFgtw ( ). pBSmttGFPgtw contains the inducible GFP-tagging cassette from pIGFgtw cloned upstream of a cycloheximide-resistance allele of the rpL29 gene, which allows the tagged construct to integrate into the endogenous rpL29 locus (Chalker, Washington University).

Sequencing:

Article Title: Basal Body Components Exhibit Differential Protein Dynamics during Nascent Basal Body Assembly
Article Snippet: .. Plasmids were constructed by cloning the coding sequence of each gene into the pENTR-D Gateway Entry Vector (Invitrogen, Carlsbad, CA). .. The coding sequence was then Gateway cloned into pBSmttGFPgtw or pIGFgtw ( ). pBSmttGFPgtw contains the inducible GFP-tagging cassette from pIGFgtw cloned upstream of a cycloheximide-resistance allele of the rpL29 gene, which allows the tagged construct to integrate into the endogenous rpL29 locus (Chalker, Washington University).

Article Title: The Nuclear Ribonucleoprotein SmD1 Interplays with Splicing, RNA Quality Control, and Posttranscriptional Gene Silencing in Arabidopsis [OPEN]
Article Snippet: .. After recombination into pENTR/D vector through the Gateway BP recombinase reaction (Invitrogen) and verification by sequencing, final recombination into pUB-DEST and pUBC-GFP through the Gateway LR recombinase reaction (Invitrogen) created pUBQ10:SmD1b and pUBQ10:SmD1b-GFP , respectively. .. For SmD1a, a 863-bp genomic fragment starting 53 bp upstream the ATG of At3g07590 and ending at the stop codon was amplified with Phusion High-Fidelity DNA polymerase (Thermo) using primers SmD1a-1 and SmD1a-2 ( ).

Transgenic Assay:

Article Title: Regulation of Cell Proliferation in the Stomatal Lineage by the Arabidopsis MYB FOUR LIPS via Direct Targeting of Core Cell Cycle Genes [W]
Article Snippet: .. ProCDC6A:GUS staining was detected as by except that leaves were cleared for 2 to 7 d. To make Pro35S:E2Fa-GFP transgenic lines, E2Fa (At2g36010) cDNA was cloned into a pENTR Gateway vector (Invitrogen), mobilized into a pGWB5 binary destination vector, and then Agrobacterium tumefaciens was transformed into plants using a floral dip method ( ). .. The ProCDKB1;1:GFP and ProCDKB1;2:GFP transcriptional fusions were generated via PCR amplification of ~0.5 and 2.9 kb, respectively, of sequence upstream of the start codon (primers shown in Supplemental Table 2 online).

Generated:

Article Title: Sfr1, a Tetrahymena thermophila Sfi1 Repeat Protein, Modulates the Production of Cortical Row Basal Bodies
Article Snippet: .. The exogenous N-terminal GFP fusion used to localize Sfr1 was generated by cloning SFR1 first into the pENTR4 Dual Selection Vector (Invitrogen) and then subcloning it into pBS-MTT1 -GFP-gtw ( ) by utilizing the Gateway cloning system (Invitrogen). ..

Article Title: Wnt/β-catenin Signaling Pathway Regulates Specific lncRNAs That Impact Dermal Fibroblasts and Skin Fibrosis
Article Snippet: .. Wincr1 lentivirus was generated by subcloning Wincr1 from pMA-T into the Nco1-EcoRV region of pENTR4 (Thermo Fisher Cat. No. A10465) vector and then swapping it into the pGK destination vector (Addgene Cat. No. 19068). .. Lentivirus was generated in 293T (f-variant) cells (ATCC Cat. No. 3216) by simultaneously transfecting pGK Destination Vector containing Wincr1 (4.5 μg), PMD2G helper plasmid (1.6 μg) (Addgene Cat. No. 12259), and pCMV-dR874 plasmid (3.2 μg), using Lipofectamine 3000 reagent (18 μL) (Invitrogen Cat. No. L30000015) in 1.5 mL Opti-MEM (Thermo Fisher Cat. No. 31985062) media in a 6 cm dish.

Article Title: A New XRCC1-Containing Complex and Its Role in Cellular Survival of Methyl Methanesulfonate Treatment
Article Snippet: .. Full-length cDNAs encoding APTX and PNK were amplified from the first-strand cDNA generated by RT-PCR using RNA isolated from HeLa cells and cloned into the Gateway PENTR TOPO vector (Invitrogen). ..

Article Title: NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila
Article Snippet: .. The UAS-NFAT[DN] transgene was generated by cloning the Rel-Homology Domain (RHD) of the NFAT gene into the pENTR-D Gateway cloning vector (Invitrogen Inc.) followed by insertion into the pTW Gateway compatible destination vector. .. Larval dissection, staining and confocal microscopy were performed according to standard protocols ( ).

Selection:

Article Title: Sfr1, a Tetrahymena thermophila Sfi1 Repeat Protein, Modulates the Production of Cortical Row Basal Bodies
Article Snippet: .. The exogenous N-terminal GFP fusion used to localize Sfr1 was generated by cloning SFR1 first into the pENTR4 Dual Selection Vector (Invitrogen) and then subcloning it into pBS-MTT1 -GFP-gtw ( ) by utilizing the Gateway cloning system (Invitrogen). ..

BAC Assay:

Article Title: BRANCHLESS TRICHOMES links cell shape and cell cycle control in Arabidopsis trichomes
Article Snippet: .. The full-length BLT coding region was PCR amplified from the BAC clone FIN19 using primers ENSIF (5′-CACCATGAAGGATATGAAGATGCAGAGC-3′) and ENS1R1 (5′-TCAAGGAGGAGGAGAGGAGAGA-3′), which contains a stop codon, or ENS1R2 (5′-AGGAGGAGGAGAGGAGAGAAGAG-3′), which lacks a stop codon, and cloned into the Gateway vector pENTR following the manufacturer's protocol (Invitrogen). .. Error-free entry clones were confirmed by DNA sequencing before attL recombination into the Gateway-compatible destination binary vector pLEELA-pGL2 ( ) for overexpression in developing trichomes.

Reverse Transcription Polymerase Chain Reaction:

Article Title: A New XRCC1-Containing Complex and Its Role in Cellular Survival of Methyl Methanesulfonate Treatment
Article Snippet: .. Full-length cDNAs encoding APTX and PNK were amplified from the first-strand cDNA generated by RT-PCR using RNA isolated from HeLa cells and cloned into the Gateway PENTR TOPO vector (Invitrogen). ..

Staining:

Article Title: Regulation of Cell Proliferation in the Stomatal Lineage by the Arabidopsis MYB FOUR LIPS via Direct Targeting of Core Cell Cycle Genes [W]
Article Snippet: .. ProCDC6A:GUS staining was detected as by except that leaves were cleared for 2 to 7 d. To make Pro35S:E2Fa-GFP transgenic lines, E2Fa (At2g36010) cDNA was cloned into a pENTR Gateway vector (Invitrogen), mobilized into a pGWB5 binary destination vector, and then Agrobacterium tumefaciens was transformed into plants using a floral dip method ( ). .. The ProCDKB1;1:GFP and ProCDKB1;2:GFP transcriptional fusions were generated via PCR amplification of ~0.5 and 2.9 kb, respectively, of sequence upstream of the start codon (primers shown in Supplemental Table 2 online).

Transformation Assay:

Article Title: Regulation of Cell Proliferation in the Stomatal Lineage by the Arabidopsis MYB FOUR LIPS via Direct Targeting of Core Cell Cycle Genes [W]
Article Snippet: .. ProCDC6A:GUS staining was detected as by except that leaves were cleared for 2 to 7 d. To make Pro35S:E2Fa-GFP transgenic lines, E2Fa (At2g36010) cDNA was cloned into a pENTR Gateway vector (Invitrogen), mobilized into a pGWB5 binary destination vector, and then Agrobacterium tumefaciens was transformed into plants using a floral dip method ( ). .. The ProCDKB1;1:GFP and ProCDKB1;2:GFP transcriptional fusions were generated via PCR amplification of ~0.5 and 2.9 kb, respectively, of sequence upstream of the start codon (primers shown in Supplemental Table 2 online).

Plasmid Preparation:

Article Title: BRANCHLESS TRICHOMES links cell shape and cell cycle control in Arabidopsis trichomes
Article Snippet: .. The full-length BLT coding region was PCR amplified from the BAC clone FIN19 using primers ENSIF (5′-CACCATGAAGGATATGAAGATGCAGAGC-3′) and ENS1R1 (5′-TCAAGGAGGAGGAGAGGAGAGA-3′), which contains a stop codon, or ENS1R2 (5′-AGGAGGAGGAGAGGAGAGAAGAG-3′), which lacks a stop codon, and cloned into the Gateway vector pENTR following the manufacturer's protocol (Invitrogen). .. Error-free entry clones were confirmed by DNA sequencing before attL recombination into the Gateway-compatible destination binary vector pLEELA-pGL2 ( ) for overexpression in developing trichomes.

Article Title: Sfr1, a Tetrahymena thermophila Sfi1 Repeat Protein, Modulates the Production of Cortical Row Basal Bodies
Article Snippet: .. The exogenous N-terminal GFP fusion used to localize Sfr1 was generated by cloning SFR1 first into the pENTR4 Dual Selection Vector (Invitrogen) and then subcloning it into pBS-MTT1 -GFP-gtw ( ) by utilizing the Gateway cloning system (Invitrogen). ..

Article Title: Wnt/β-catenin Signaling Pathway Regulates Specific lncRNAs That Impact Dermal Fibroblasts and Skin Fibrosis
Article Snippet: .. Wincr1 lentivirus was generated by subcloning Wincr1 from pMA-T into the Nco1-EcoRV region of pENTR4 (Thermo Fisher Cat. No. A10465) vector and then swapping it into the pGK destination vector (Addgene Cat. No. 19068). .. Lentivirus was generated in 293T (f-variant) cells (ATCC Cat. No. 3216) by simultaneously transfecting pGK Destination Vector containing Wincr1 (4.5 μg), PMD2G helper plasmid (1.6 μg) (Addgene Cat. No. 12259), and pCMV-dR874 plasmid (3.2 μg), using Lipofectamine 3000 reagent (18 μL) (Invitrogen Cat. No. L30000015) in 1.5 mL Opti-MEM (Thermo Fisher Cat. No. 31985062) media in a 6 cm dish.

Article Title: Basal Body Components Exhibit Differential Protein Dynamics during Nascent Basal Body Assembly
Article Snippet: .. Plasmids were constructed by cloning the coding sequence of each gene into the pENTR-D Gateway Entry Vector (Invitrogen, Carlsbad, CA). .. The coding sequence was then Gateway cloned into pBSmttGFPgtw or pIGFgtw ( ). pBSmttGFPgtw contains the inducible GFP-tagging cassette from pIGFgtw cloned upstream of a cycloheximide-resistance allele of the rpL29 gene, which allows the tagged construct to integrate into the endogenous rpL29 locus (Chalker, Washington University).

Article Title: The Nuclear Ribonucleoprotein SmD1 Interplays with Splicing, RNA Quality Control, and Posttranscriptional Gene Silencing in Arabidopsis [OPEN]
Article Snippet: .. After recombination into pENTR/D vector through the Gateway BP recombinase reaction (Invitrogen) and verification by sequencing, final recombination into pUB-DEST and pUBC-GFP through the Gateway LR recombinase reaction (Invitrogen) created pUBQ10:SmD1b and pUBQ10:SmD1b-GFP , respectively. .. For SmD1a, a 863-bp genomic fragment starting 53 bp upstream the ATG of At3g07590 and ending at the stop codon was amplified with Phusion High-Fidelity DNA polymerase (Thermo) using primers SmD1a-1 and SmD1a-2 ( ).

Article Title: Regulation of Cell Proliferation in the Stomatal Lineage by the Arabidopsis MYB FOUR LIPS via Direct Targeting of Core Cell Cycle Genes [W]
Article Snippet: .. ProCDC6A:GUS staining was detected as by except that leaves were cleared for 2 to 7 d. To make Pro35S:E2Fa-GFP transgenic lines, E2Fa (At2g36010) cDNA was cloned into a pENTR Gateway vector (Invitrogen), mobilized into a pGWB5 binary destination vector, and then Agrobacterium tumefaciens was transformed into plants using a floral dip method ( ). .. The ProCDKB1;1:GFP and ProCDKB1;2:GFP transcriptional fusions were generated via PCR amplification of ~0.5 and 2.9 kb, respectively, of sequence upstream of the start codon (primers shown in Supplemental Table 2 online).

Article Title: A New XRCC1-Containing Complex and Its Role in Cellular Survival of Methyl Methanesulfonate Treatment
Article Snippet: .. Full-length cDNAs encoding APTX and PNK were amplified from the first-strand cDNA generated by RT-PCR using RNA isolated from HeLa cells and cloned into the Gateway PENTR TOPO vector (Invitrogen). ..

Article Title: NFAT regulates pre-synaptic development and activity-dependent plasticity in Drosophila
Article Snippet: .. The UAS-NFAT[DN] transgene was generated by cloning the Rel-Homology Domain (RHD) of the NFAT gene into the pENTR-D Gateway cloning vector (Invitrogen Inc.) followed by insertion into the pTW Gateway compatible destination vector. .. Larval dissection, staining and confocal microscopy were performed according to standard protocols ( ).

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    Thermo Fisher pentr d topo entry vectors
    Pentr D Topo Entry Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr d topo entry vectors/product/Thermo Fisher
    Average 85 stars, based on 987 article reviews
    Price from $9.99 to $1999.99
    pentr d topo entry vectors - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Thermo Fisher pentr topo d vector
    Pentr Topo D Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pentr topo d vector/product/Thermo Fisher
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pentr topo d vector - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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