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In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth <t>muscle</t> <t>cells.</t> <t>4′,6-Diamidine-2-phenylindole</t> (DAPI) = nuclei. Scale bar = 50 μm.
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In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth <t>muscle</t> <t>cells.</t> <t>4′,6-Diamidine-2-phenylindole</t> (DAPI) = nuclei. Scale bar = 50 μm.
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In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth <t>muscle</t> <t>cells.</t> <t>4′,6-Diamidine-2-phenylindole</t> (DAPI) = nuclei. Scale bar = 50 μm.
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The S100B inhibitor <t>pentamidine</t> in combination with BEV improves the ovarian cancer response compared to BEV alone. (A) Flowchart of the in vivo experiment. Two weeks after intraperitoneal inoculation of ovarian cancer cells, drug treatment was administered. The mice were randomly divided into a normal saline treatment control group, BEV treatment group, pentamidine treatment group, and BEV with pentamidine treatment group. (B) Line chart of the tumor fluorescence intensity of the four groups of mice. (C) Tumor fluorescence imaging of mice after tumor formation (week 2), after killing the control group and pentamidine treatment group (week 4–6), after killing the BEV treatment group (week 8–9), and after killing the BEV with pentamidine treatment group (week 13–14). (D) Kaplan–Meier survival curves of the four groups of mice. (E) CD31 immunohistochemical staining and (F) MVD statistics of tumor tissue from the four groups of mice. (E) S100B immunohistochemical staining and S100B (G) staining intensity of tumor tissue from the four groups of mice. BEV: Bevacizumab; MVD: Micro-vessel density; *: P < 0.05; **: P < 0.01;^: P < 0.05 vs. NC; #: P < 0.05 vs. pentamidine; &: P < 0.05 vs. BEV.
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In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth muscle cells. 4′,6-Diamidine-2-phenylindole (DAPI) = nuclei. Scale bar = 50 μm.

Journal: Biomaterials Research

Article Title: Exploring Perivascular Adipose Tissue Responses to Bioresorbable Thermoplastic Polyurethane Vascular Grafts

doi: 10.34133/bmr.0372

Figure Lengend Snippet: In vitro-seeded cells on thermoplastic polyurethane (TPU) vs. CTRL. (A) Scanning electron microscopy (SEM) analysis of in vitro-seeded cells on TPU specimens vs. glass (CTRL). Scale bar = 20 μm. (B to D) Immunofluorescence (IF) staining showing the expression of cell-specific markers. PVAT, perivascular adipose tissue (PVAT)-derived cells; ECs, endothelial cells; SMCs, smooth muscle cells. 4′,6-Diamidine-2-phenylindole (DAPI) = nuclei. Scale bar = 50 μm.

Article Snippet: Subsequently, samples were again washed with PBS/1% BSA, placed on microscopy slides, and embedded in 4′,6-diamidine-2-phenylindole (DAPI; Thermo Fisher Scientific, Austria) containing a mounting medium (Agilent Technologies, Austria).

Techniques: In Vitro, Electron Microscopy, Immunofluorescence, Staining, Expressing, Derivative Assay

The S100B inhibitor pentamidine in combination with BEV improves the ovarian cancer response compared to BEV alone. (A) Flowchart of the in vivo experiment. Two weeks after intraperitoneal inoculation of ovarian cancer cells, drug treatment was administered. The mice were randomly divided into a normal saline treatment control group, BEV treatment group, pentamidine treatment group, and BEV with pentamidine treatment group. (B) Line chart of the tumor fluorescence intensity of the four groups of mice. (C) Tumor fluorescence imaging of mice after tumor formation (week 2), after killing the control group and pentamidine treatment group (week 4–6), after killing the BEV treatment group (week 8–9), and after killing the BEV with pentamidine treatment group (week 13–14). (D) Kaplan–Meier survival curves of the four groups of mice. (E) CD31 immunohistochemical staining and (F) MVD statistics of tumor tissue from the four groups of mice. (E) S100B immunohistochemical staining and S100B (G) staining intensity of tumor tissue from the four groups of mice. BEV: Bevacizumab; MVD: Micro-vessel density; *: P < 0.05; **: P < 0.01;^: P < 0.05 vs. NC; #: P < 0.05 vs. pentamidine; &: P < 0.05 vs. BEV.

Journal: Journal of Advanced Research

Article Title: S100B induces angiogenesis via the clathrin/FOXO1/β-catenin signaling pathway and contributes to Bevacizumab resistance in epithelial ovarian cancer

doi: 10.1016/j.jare.2025.05.060

Figure Lengend Snippet: The S100B inhibitor pentamidine in combination with BEV improves the ovarian cancer response compared to BEV alone. (A) Flowchart of the in vivo experiment. Two weeks after intraperitoneal inoculation of ovarian cancer cells, drug treatment was administered. The mice were randomly divided into a normal saline treatment control group, BEV treatment group, pentamidine treatment group, and BEV with pentamidine treatment group. (B) Line chart of the tumor fluorescence intensity of the four groups of mice. (C) Tumor fluorescence imaging of mice after tumor formation (week 2), after killing the control group and pentamidine treatment group (week 4–6), after killing the BEV treatment group (week 8–9), and after killing the BEV with pentamidine treatment group (week 13–14). (D) Kaplan–Meier survival curves of the four groups of mice. (E) CD31 immunohistochemical staining and (F) MVD statistics of tumor tissue from the four groups of mice. (E) S100B immunohistochemical staining and S100B (G) staining intensity of tumor tissue from the four groups of mice. BEV: Bevacizumab; MVD: Micro-vessel density; *: P < 0.05; **: P < 0.01;^: P < 0.05 vs. NC; #: P < 0.05 vs. pentamidine; &: P < 0.05 vs. BEV.

Article Snippet: The pentamidine treatment group was administered pentamidine (Selleck, China) at a dosage of 10 mg/kg intraperitoneally three times a week [ ].

Techniques: In Vivo, Saline, Control, Fluorescence, Imaging, Immunohistochemical staining, Staining