Journal: Lipids in Health and Disease
Article Title: Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells
Figure Lengend Snippet: Effect of polyunsaturated fatty acids on MMP-9 mRNA expression . MonoMac 6 cells (4 × 10 6 cells/5 ml X-Vivo 15 on Nunclon 60 mm dishes) were treated with 1 or 10 μM arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosapentaenoic acid (DPA) for 24 h. Then the cells were pelleted by centrifugation (400 × G, +4°C) and washed with ice-cold PBS. Total cellular RNA was isolated, reverse transcribed and the resulting cDNA was amplified as described in the methods. The MMP-9 expression was demonstrated by real-time PCR. Results represent the means ± SEM of four independent experiments. Only 10 μM AA increased the expression of MMP-9 mRNA statistically significantly.
Article Snippet: For the experiments, cells were harvested, washed with phosphate buffered saline (PBS), pH 7.4, and seeded on 12-well Nunclon plates (Nunc A/S, Denmark) at 0.8 × 106 cells/ml in serum-free medium X-Vivo 15 (BioWhittaker, Belgium) supplemented with penicillin-streptomycin.
Techniques: Expressing, Centrifugation, Isolation, Amplification, Real-time Polymerase Chain Reaction