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S D Fine-Chem chloroform
Chloroform, supplied by S D Fine-Chem, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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chloroform - by Bioz Stars, 2020-08
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Article Snippet: Chemicals 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye powder (Serva Electrophoresis), dimethyl sulfoxide cell culture grade (BioWorld), amphotericin B (HiMedia), penicillin and streptomycin solution stabilized (Sigma), ethylene di-amine tetra-acetic acid (EDTA; MP Biomedicals), DPBS/modified 1X (Dulbecoo's phosphate buffer saline without Ca+ and Mg+ ) (HiMedia), fetal bovine serum (Quaditive), chloroform (SD Fine Chem).

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    Thermo Fisher phosphate buffered saline pbs
    Effect of polyunsaturated fatty acids on MMP-9 mRNA expression . MonoMac 6 cells (4 × 10 6 cells/5 ml X-Vivo 15 on <t>Nunclon</t> 60 mm dishes) were treated with 1 or 10 μM arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosapentaenoic acid (DPA) for 24 h. Then the cells were pelleted by centrifugation (400 × G, +4°C) and washed with ice-cold <t>PBS.</t> Total cellular RNA was isolated, reverse transcribed and the resulting cDNA was amplified as described in the methods. The MMP-9 expression was demonstrated by real-time PCR. Results represent the means ± SEM of four independent experiments. Only 10 μM AA increased the expression of MMP-9 mRNA statistically significantly.
    Phosphate Buffered Saline Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    NeuroSearch A/S gaba sniffer human embryonic kidney hek cells 293 expressing gabaa receptors
    Whole-cell <t>sniffer</t> with <t>GABA</t> A receptors. (A) RT-PCR verifying the expression of GABA A receptors composed of α1β2γ2 subunits in <t>HEK</t> cells. Primers for α6 and δ subunits were included as control. Expression of hGAPDH served as control (C) of cDNA quality. (B) Whole-cell recording of a HEK cell detached from the coverslip. (C) A single puff of GABA evoked a large outward current. (D1) Distribution of the amplitude of the evoked currents. 82% (14/17) of the HEK cells tested with GABA responded with large outward currents. (D2) Amplitude of the current evoked by repetitive GABA puffs applied every 60 s. The response was stable over hours ( n = 2). (E) Response of two sniffers to GABA. The evoked current was suppressed by gabazine (10 μM) or by further addition of picrotoxin (50 μM).
    Gaba Sniffer Human Embryonic Kidney Hek Cells 293 Expressing Gabaa Receptors, supplied by NeuroSearch A/S, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gaba sniffer human embryonic kidney hek cells 293 expressing gabaa receptors/product/NeuroSearch A/S
    Average 86 stars, based on 1 article reviews
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    88
    Thermo Fisher human fibrosarcoma ht1080 cells
    Effect of brown algae extracts on the gelatinolytic activities of MMP-2 and MMP-9 in PMA-stimulated (10 ng/mL) <t>HT1080</t> cells determined by gelatin zymography (A). Gelatinolytic activities of MMP-2 and MMP-9 in conditioned media were detected by SDS-PAGE
    Human Fibrosarcoma Ht1080 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of polyunsaturated fatty acids on MMP-9 mRNA expression . MonoMac 6 cells (4 × 10 6 cells/5 ml X-Vivo 15 on Nunclon 60 mm dishes) were treated with 1 or 10 μM arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosapentaenoic acid (DPA) for 24 h. Then the cells were pelleted by centrifugation (400 × G, +4°C) and washed with ice-cold PBS. Total cellular RNA was isolated, reverse transcribed and the resulting cDNA was amplified as described in the methods. The MMP-9 expression was demonstrated by real-time PCR. Results represent the means ± SEM of four independent experiments. Only 10 μM AA increased the expression of MMP-9 mRNA statistically significantly.

    Journal: Lipids in Health and Disease

    Article Title: Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells

    doi: 10.1186/1476-511X-8-11

    Figure Lengend Snippet: Effect of polyunsaturated fatty acids on MMP-9 mRNA expression . MonoMac 6 cells (4 × 10 6 cells/5 ml X-Vivo 15 on Nunclon 60 mm dishes) were treated with 1 or 10 μM arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosapentaenoic acid (DPA) for 24 h. Then the cells were pelleted by centrifugation (400 × G, +4°C) and washed with ice-cold PBS. Total cellular RNA was isolated, reverse transcribed and the resulting cDNA was amplified as described in the methods. The MMP-9 expression was demonstrated by real-time PCR. Results represent the means ± SEM of four independent experiments. Only 10 μM AA increased the expression of MMP-9 mRNA statistically significantly.

    Article Snippet: For the experiments, cells were harvested, washed with phosphate buffered saline (PBS), pH 7.4, and seeded on 12-well Nunclon plates (Nunc A/S, Denmark) at 0.8 × 106 cells/ml in serum-free medium X-Vivo 15 (BioWhittaker, Belgium) supplemented with penicillin-streptomycin.

    Techniques: Expressing, Centrifugation, Isolation, Amplification, Real-time Polymerase Chain Reaction

    Whole-cell sniffer with GABA A receptors. (A) RT-PCR verifying the expression of GABA A receptors composed of α1β2γ2 subunits in HEK cells. Primers for α6 and δ subunits were included as control. Expression of hGAPDH served as control (C) of cDNA quality. (B) Whole-cell recording of a HEK cell detached from the coverslip. (C) A single puff of GABA evoked a large outward current. (D1) Distribution of the amplitude of the evoked currents. 82% (14/17) of the HEK cells tested with GABA responded with large outward currents. (D2) Amplitude of the current evoked by repetitive GABA puffs applied every 60 s. The response was stable over hours ( n = 2). (E) Response of two sniffers to GABA. The evoked current was suppressed by gabazine (10 μM) or by further addition of picrotoxin (50 μM).

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Fast detection of extrasynaptic GABA with a whole-cell sniffer

    doi: 10.3389/fncel.2014.00133

    Figure Lengend Snippet: Whole-cell sniffer with GABA A receptors. (A) RT-PCR verifying the expression of GABA A receptors composed of α1β2γ2 subunits in HEK cells. Primers for α6 and δ subunits were included as control. Expression of hGAPDH served as control (C) of cDNA quality. (B) Whole-cell recording of a HEK cell detached from the coverslip. (C) A single puff of GABA evoked a large outward current. (D1) Distribution of the amplitude of the evoked currents. 82% (14/17) of the HEK cells tested with GABA responded with large outward currents. (D2) Amplitude of the current evoked by repetitive GABA puffs applied every 60 s. The response was stable over hours ( n = 2). (E) Response of two sniffers to GABA. The evoked current was suppressed by gabazine (10 μM) or by further addition of picrotoxin (50 μM).

    Article Snippet: GABA SNIFFER Human embryonic kidney (HEK) cells 293 expressing GABAA receptors (gift from NeuroSearch A/S, Ballerup, Denmark) were grown in T75 flasks (VWR, Herlev, Denmark) in full DMEM media (DMEM supplemented with 100 U/mL penicillin, 100 mg/ml streptomycin, and 10% fetal calf serum; (Sigma-Aldrich, Copenhagen, Denmark) at 37°C, in a humidified atmosphere with 5% CO2 .

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    Effect of brown algae extracts on the gelatinolytic activities of MMP-2 and MMP-9 in PMA-stimulated (10 ng/mL) HT1080 cells determined by gelatin zymography (A). Gelatinolytic activities of MMP-2 and MMP-9 in conditioned media were detected by SDS-PAGE

    Journal: Preventive Nutrition and Food Science

    Article Title: Evaluation of Effective MMP Inhibitors from Eight Different Brown Algae in Human Fibrosarcoma HT1080 Cells

    doi: 10.3746/pnf.2015.20.3.153

    Figure Lengend Snippet: Effect of brown algae extracts on the gelatinolytic activities of MMP-2 and MMP-9 in PMA-stimulated (10 ng/mL) HT1080 cells determined by gelatin zymography (A). Gelatinolytic activities of MMP-2 and MMP-9 in conditioned media were detected by SDS-PAGE

    Article Snippet: Human fibrosarcoma HT1080 cells (Korean Cell Line Bank, Seoul, Korea) were grown as monolayers in T-75 tissue culture flasks (Nunc A/S, Roskilde, Denmark) at 5% CO2 and 37°C humidified atmosphere using Dulbecco’s modified eagle medium (DMEM, Gibco-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum, 2 mM glutamine, and 100 μg/mL penicillin-streptomycin (Gibco-BRL).

    Techniques: Zymography, SDS Page

    Cytotoxicity of eight brown algae extract treatments for 24 h (A) and 48 h (B) on human fibrosarcoma cell line HT1080 assessed by the MTT-formazan assay at the concentrations of 10, 50, and 100 μg/mL. Cell viability was indicated as percentage

    Journal: Preventive Nutrition and Food Science

    Article Title: Evaluation of Effective MMP Inhibitors from Eight Different Brown Algae in Human Fibrosarcoma HT1080 Cells

    doi: 10.3746/pnf.2015.20.3.153

    Figure Lengend Snippet: Cytotoxicity of eight brown algae extract treatments for 24 h (A) and 48 h (B) on human fibrosarcoma cell line HT1080 assessed by the MTT-formazan assay at the concentrations of 10, 50, and 100 μg/mL. Cell viability was indicated as percentage

    Article Snippet: Human fibrosarcoma HT1080 cells (Korean Cell Line Bank, Seoul, Korea) were grown as monolayers in T-75 tissue culture flasks (Nunc A/S, Roskilde, Denmark) at 5% CO2 and 37°C humidified atmosphere using Dulbecco’s modified eagle medium (DMEM, Gibco-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum, 2 mM glutamine, and 100 μg/mL penicillin-streptomycin (Gibco-BRL).

    Techniques: MTT Assay

    Effect of brown algae extracts on mRNA expression levels of MMP-2, -9 (A) and TIMP-1, -2 (B) in HT1080 cells stimulated with PMA (10 ng/mL). Cells were treated with different concentrations of sample and PMA (10 ng/mL) for 24 h. The expression levels

    Journal: Preventive Nutrition and Food Science

    Article Title: Evaluation of Effective MMP Inhibitors from Eight Different Brown Algae in Human Fibrosarcoma HT1080 Cells

    doi: 10.3746/pnf.2015.20.3.153

    Figure Lengend Snippet: Effect of brown algae extracts on mRNA expression levels of MMP-2, -9 (A) and TIMP-1, -2 (B) in HT1080 cells stimulated with PMA (10 ng/mL). Cells were treated with different concentrations of sample and PMA (10 ng/mL) for 24 h. The expression levels

    Article Snippet: Human fibrosarcoma HT1080 cells (Korean Cell Line Bank, Seoul, Korea) were grown as monolayers in T-75 tissue culture flasks (Nunc A/S, Roskilde, Denmark) at 5% CO2 and 37°C humidified atmosphere using Dulbecco’s modified eagle medium (DMEM, Gibco-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum, 2 mM glutamine, and 100 μg/mL penicillin-streptomycin (Gibco-BRL).

    Techniques: Expressing

    Effect of brown algae extracts on the migration of HT1080 cells after 24 h incubation. HT1080 cells were grown to 80% confluence prior to the application of 2 mm wide injury line. Pictures of cell migrating towards the injury line were taken at the start

    Journal: Preventive Nutrition and Food Science

    Article Title: Evaluation of Effective MMP Inhibitors from Eight Different Brown Algae in Human Fibrosarcoma HT1080 Cells

    doi: 10.3746/pnf.2015.20.3.153

    Figure Lengend Snippet: Effect of brown algae extracts on the migration of HT1080 cells after 24 h incubation. HT1080 cells were grown to 80% confluence prior to the application of 2 mm wide injury line. Pictures of cell migrating towards the injury line were taken at the start

    Article Snippet: Human fibrosarcoma HT1080 cells (Korean Cell Line Bank, Seoul, Korea) were grown as monolayers in T-75 tissue culture flasks (Nunc A/S, Roskilde, Denmark) at 5% CO2 and 37°C humidified atmosphere using Dulbecco’s modified eagle medium (DMEM, Gibco-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum, 2 mM glutamine, and 100 μg/mL penicillin-streptomycin (Gibco-BRL).

    Techniques: Migration, Incubation