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PAA Laboratories penicillin streptomycin
Penicillin Streptomycin, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/penicillin streptomycin/product/PAA Laboratories
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
penicillin streptomycin - by Bioz Stars, 2021-03
86/100 stars

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Cell Culture:

Article Title: Human Stefin B Role in Cell's Response to Misfolded Proteins and Autophagy
Article Snippet: Sequencing grade modified trypsin (V5117) and 12% precast gels (59509) for LC-MS/MS analysis were purchased from Promega Corporation (Fitchburg, WI, USA) and Lonza (Basel, Switzerland), respectively. .. For cell culture experiments, Dulbecco's modified Eagle's medium with high glucose and L-glutamine (E15-810), heat inactivated fetal bovine serum (FBS) (A15-104), penicillin/streptomycin (100×) (P11-010), Dulbecco's phosphate buffer saline (PBS) (H15-001), L-glutamine (M11-004), trypsin EDTA (L11-658, all PAA Laboratories GmbH, Pasching, Austria), and TrypLE Select (0040090DG, Gibco, Invitrogen, Carlsbad, CA, USA) were used. .. The following primary antibodies were used: anti-actin (A3852, Sigma Aldrich, St. Louis, MO, USA), anti-LC3 (ab78078 and ab58610, Abcam, Cambridge, UK) and anti-p62 (MABC32, Millipore, Millipore, Billerica, MA, USA).

Article Title: Paclitaxel resistance increases oncolytic adenovirus efficacy via upregulated CAR expression and dysfunctional cell cycle control), Paclitaxel resistance increases oncolytic adenovirus efficacy via upregulated CAR expression and dysfunctional cell cycle control
Article Snippet: Cell lines underwent 16 locus STR validation (LGC Standards, Middlesex UK) in 2011 and were most recently revalidated by 10 locus STR validation in June 2014 (CRUK Beatson Institute, Glasgow, UK; ). .. A2780 and A2780tx1000 cells were cultured in DMEM plus penicillin/streptomycin and 10% FCS, (PAA Laboratories, Austria). .. SKOV3 and SKOV3‐TR cells were cultured in RPMI plus penicillin/streptomycin and 10% FCS.

Article Title: Structures of the APC–ARM domain in complexes with discrete Amer1/WTX fragments reveal that it uses a consensus mode to recognize its binding partners
Article Snippet: Molecular graphics All protein structure figures were generated with PyMOL ( http://www.pymol.org ). .. Cell culture and transfection All cell lines were cultured in DMEM (PAA-Laboratories, Dartmouth, MA, USA) supplemented with 10% fetal calf serum (Perbio Science, Northumberland, UK) and 1% penicillin/streptomycin (PAA-Laboratories) at 37 °C in a humidified atmosphere of 10% CO2 . .. Transient plasmid transfections were performed using polyethyleneimine (Sigma-Aldrich, St Louis, MO, USA) for HEK293T cells (3 μg of each plasmid) or TransIT-TKO (Mirus Bio LLC, Madison, WI, USA) for MCF-7 cells (1 μg of each plasmid).

Article Title: Inhibition of Autolysis by Lipase LipA in Streptococcus pneumoniae Sepsis
Article Snippet: The murine macrophage RAW 264.7 and lung carcinoma A549 epithelial cell lines were obtained from the American Type Culture Collection. .. Cells were cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum (FBS; Gibco) and 1X penicillin/streptomycin (PAA Laboratories GmbH) ( ). .. Cloning and purification of recombinant D39 spd_1447 and WU2 spd_1447 The gene encoding spd_1447 (lipA) was amplified from the D39 or WU2 genome using primers ( ) that contained Hin dIII and Bam HI restriction enzyme sites.

Modification:

Article Title: Human Stefin B Role in Cell's Response to Misfolded Proteins and Autophagy
Article Snippet: Sequencing grade modified trypsin (V5117) and 12% precast gels (59509) for LC-MS/MS analysis were purchased from Promega Corporation (Fitchburg, WI, USA) and Lonza (Basel, Switzerland), respectively. .. For cell culture experiments, Dulbecco's modified Eagle's medium with high glucose and L-glutamine (E15-810), heat inactivated fetal bovine serum (FBS) (A15-104), penicillin/streptomycin (100×) (P11-010), Dulbecco's phosphate buffer saline (PBS) (H15-001), L-glutamine (M11-004), trypsin EDTA (L11-658, all PAA Laboratories GmbH, Pasching, Austria), and TrypLE Select (0040090DG, Gibco, Invitrogen, Carlsbad, CA, USA) were used. .. The following primary antibodies were used: anti-actin (A3852, Sigma Aldrich, St. Louis, MO, USA), anti-LC3 (ab78078 and ab58610, Abcam, Cambridge, UK) and anti-p62 (MABC32, Millipore, Millipore, Billerica, MA, USA).

Article Title: Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial
Article Snippet: .. Adipose tissue was stored in MSC medium containing 1 g/l Dulbecco’s Modified Eagle’s Medium (DMEM) with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 1% l -glutamine (DMEM with 25 mM HEPES, with l -glutamine; PAA Laboratories, Pasching, Austria), fetal bovine serum (PAA Laboratories) and 1% penicillin/streptomycin (Penicillin-Streptomycin 100; PAA Laboratories) at 5 °C and shipped to the laboratory overnight. .. AT-MSC isolation and culture Adipose tissue was mechanically separated using a scalpel blade and a tissue chopper.

Article Title: Inhibition of Autolysis by Lipase LipA in Streptococcus pneumoniae Sepsis
Article Snippet: The murine macrophage RAW 264.7 and lung carcinoma A549 epithelial cell lines were obtained from the American Type Culture Collection. .. Cells were cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum (FBS; Gibco) and 1X penicillin/streptomycin (PAA Laboratories GmbH) ( ). .. Cloning and purification of recombinant D39 spd_1447 and WU2 spd_1447 The gene encoding spd_1447 (lipA) was amplified from the D39 or WU2 genome using primers ( ) that contained Hin dIII and Bam HI restriction enzyme sites.

Article Title: TLR4 Mediates Pneumolysin-Induced ATF3 Expression through the JNK/p38 Pathway in Streptococcus pneumoniae-Infected RAW 264.7 Cells
Article Snippet: RAW 264.7 murine macrophage-like cells were obtained from the American Type Culture Collection. .. The cells were maintained at 37°C in a humidified incubator at 95% air-5% CO2 and grown in Dulbecco’s modified Eagle’s medium (DMEM; GE Healthcare, UK) supplemented with 10% fetal bovine serum (Bioscience) along with 1X penicillin/streptomycin (PAA Laboratories GmbH, Germany) ( ). .. Lipopolysaccharide (serotype 026:B6, Escherichia coli ) was purchased from Sigma (USA).

Transfection:

Article Title: Structures of the APC–ARM domain in complexes with discrete Amer1/WTX fragments reveal that it uses a consensus mode to recognize its binding partners
Article Snippet: Molecular graphics All protein structure figures were generated with PyMOL ( http://www.pymol.org ). .. Cell culture and transfection All cell lines were cultured in DMEM (PAA-Laboratories, Dartmouth, MA, USA) supplemented with 10% fetal calf serum (Perbio Science, Northumberland, UK) and 1% penicillin/streptomycin (PAA-Laboratories) at 37 °C in a humidified atmosphere of 10% CO2 . .. Transient plasmid transfections were performed using polyethyleneimine (Sigma-Aldrich, St Louis, MO, USA) for HEK293T cells (3 μg of each plasmid) or TransIT-TKO (Mirus Bio LLC, Madison, WI, USA) for MCF-7 cells (1 μg of each plasmid).

other:

Article Title: Dynamics of chemosensitivity and chromosomal instability in recurrent glioblastoma
Article Snippet: Patches from non-necrotic parts of the tumour were obtained during surgery, minced mechanically and transferred into culture flasks, containing growth medium (RPMI 1640, 20% fetal calf serum (FCS), 1% glutamine, 1% penicillin/streptomycine; PAA Laboratories, Linz, Austria).

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    PAA Laboratories glutamine
    Glutamine, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutamine/product/PAA Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glutamine - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    PAA Laboratories penicillin streptomycin
    Penicillin Streptomycin, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin streptomycin/product/PAA Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    PAA Laboratories streptomycin
    Streptomycin, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptomycin/product/PAA Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptomycin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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