penicillin streptomycin  (Mediatech)

 
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    Mediatech penicillin streptomycin
    Penicillin Streptomycin, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/penicillin streptomycin/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin - by Bioz Stars, 2021-03
    86/100 stars

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    Incubation:

    Article Title: Formulation and Characterization of Poloxamine-based Hydrogels as Tissue Sealants
    Article Snippet: .. For extract testing, the samples were incubated for 24 hours at 37 °C in 0.5 ml culture medium [DMEM/F12 (Mediatech, Manassas, VA), 10% bovine growth serum (Hyclone, Logan, UT) and 1X penicillin/streptomycin (Mediatech)]. ..

    Article Title: The common γ-chain cytokine IL-7 promotes immunopathogenesis during fungal asthma
    Article Snippet: WT BL/6 and IL22 Cre R26ReYFP reporter mice were subjected to fungal asthma. .. At 24 h after the last challenge, lungs were collected and minced in Iscove’s modified Dulbecco’s medium (Sigma, St. Louis, MO) supplemented with 1% penicillin𠄼streptomycin𠄼glutamine (Mediatech, Herndon, VA), 10% heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA) and 0.4 mg/ml polymyxin B (Thermo Fisher), followed by incubation for 60 min with tissue culture-grade type IV collagenase (1 mg/ml; Sigma, St. Louis, MO) in a 37 °C orbital shaker at 100 rpm. .. The cell suspension was filtered through sterile 70 μm nylon filters, red blood cells lysed with ACK buffer (Lonza, Walkersville, MD), then filtered through a sterile 40 μm nylon filters to create single-cell preparations.

    Cell Culture:

    Article Title: Broad T-Cell Receptor Repertoire in T-Lymphocytes Derived from Human Induced Pluripotent Stem Cells
    Article Snippet: All of the oligos used in this study were synthesized by Integrated DNA Technologies (IDT). .. Cell Culture ESC and iPSC cells were cultured on mitomycin C-treated murine embryonic fibroblasts (MEFs, derived from E14.5 CF-1 embryos) in ES cell media consisting of DMEM F-12 supplemented with 1X non-essential amino acids, 1X penicillin-streptomycin, 1X L-glutamine (all from Mediatech), 20% knock serum replacement (Invitrogen), 2-ME (Sigma) and 5 ng/ml bFGF (Invitrogen). .. Human primary keratinocytes were cultured in DermaLife K Medium Complete Kit (LifeLine Cell Technology).

    Article Title: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.
    Article Snippet: Detection of Lactobacillus spp. and G. vaginalis adhered to HeLa cell line The application of cellular lines is a standard procedure that has already been used to mimic vaginal epithelium at several in vitro studies [ - ]. .. So, HeLa epithelial cells (from American Tissue Culture Collection, ATCC) were cultured at 37°C, in 5% CO2 (vol/vol), in Dulbecco’s modified Eagle’s medium (DMEM; Quality Biological, USA) supplemented with 10% FBS (vol/vol) and 1 IU penicillin/streptomycin ml−1 (MediaTech, Germany). .. Aliquots of 1ml from HeLa epithelial cells were seeded into 24-well tissue culture plates (Frilabo, Portugal) containing glass slides (12 mm) at a density of 2×105 cells per well, and incubated at 37°C and 5% CO2 (vol/vol) until the formation of a cell monolayer.

    Article Title: Correspondence: Oncogenic MYC persistently upregulates the molecular clock component REV-ERBα
    Article Snippet: Media was supplemented with 10% fetal bovine serum (FBS, HyClone, Logan, UT, USA or Life Technologies) and 1X Penicillin/Streptomycin (Mediatech). mHCC cells were additionally supplemented with 2 mM glutamine (Mediatech), 1 mM sodium pyruvate (Mediatech) and 1X MEM non-essential amino acids (Life Technologies). .. P493-6 cells were cultured in RPMI-1640 medium (Mediatech) with 10% FBS (HyClone or Life Technologies) and 1X Penicillin/Streptomycin (Mediatech). .. All cell culture was conducted in a 5% CO2 humidified atmosphere.

    Derivative Assay:

    Article Title: Broad T-Cell Receptor Repertoire in T-Lymphocytes Derived from Human Induced Pluripotent Stem Cells
    Article Snippet: All of the oligos used in this study were synthesized by Integrated DNA Technologies (IDT). .. Cell Culture ESC and iPSC cells were cultured on mitomycin C-treated murine embryonic fibroblasts (MEFs, derived from E14.5 CF-1 embryos) in ES cell media consisting of DMEM F-12 supplemented with 1X non-essential amino acids, 1X penicillin-streptomycin, 1X L-glutamine (all from Mediatech), 20% knock serum replacement (Invitrogen), 2-ME (Sigma) and 5 ng/ml bFGF (Invitrogen). .. Human primary keratinocytes were cultured in DermaLife K Medium Complete Kit (LifeLine Cell Technology).

    Modification:

    Article Title: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.
    Article Snippet: Detection of Lactobacillus spp. and G. vaginalis adhered to HeLa cell line The application of cellular lines is a standard procedure that has already been used to mimic vaginal epithelium at several in vitro studies [ - ]. .. So, HeLa epithelial cells (from American Tissue Culture Collection, ATCC) were cultured at 37°C, in 5% CO2 (vol/vol), in Dulbecco’s modified Eagle’s medium (DMEM; Quality Biological, USA) supplemented with 10% FBS (vol/vol) and 1 IU penicillin/streptomycin ml−1 (MediaTech, Germany). .. Aliquots of 1ml from HeLa epithelial cells were seeded into 24-well tissue culture plates (Frilabo, Portugal) containing glass slides (12 mm) at a density of 2×105 cells per well, and incubated at 37°C and 5% CO2 (vol/vol) until the formation of a cell monolayer.

    Article Title: The common γ-chain cytokine IL-7 promotes immunopathogenesis during fungal asthma
    Article Snippet: WT BL/6 and IL22 Cre R26ReYFP reporter mice were subjected to fungal asthma. .. At 24 h after the last challenge, lungs were collected and minced in Iscove’s modified Dulbecco’s medium (Sigma, St. Louis, MO) supplemented with 1% penicillin𠄼streptomycin𠄼glutamine (Mediatech, Herndon, VA), 10% heat-inactivated fetal bovine serum (Invitrogen, Carlsbad, CA) and 0.4 mg/ml polymyxin B (Thermo Fisher), followed by incubation for 60 min with tissue culture-grade type IV collagenase (1 mg/ml; Sigma, St. Louis, MO) in a 37 °C orbital shaker at 100 rpm. .. The cell suspension was filtered through sterile 70 μm nylon filters, red blood cells lysed with ACK buffer (Lonza, Walkersville, MD), then filtered through a sterile 40 μm nylon filters to create single-cell preparations.

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    • streptomycin  (Mediatech)
    86
    Mediatech streptomycin
    Streptomycin, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptomycin/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    streptomycin - by Bioz Stars, 2021-03
    86/100 stars
    		  Buy from Supplier

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