penicillin streptomycin  (Lonza)


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    Name:
    Penicillin Streptomycin L Glutamine Mixture
    Description:
    Pen Strep L Glutamin mixture contains 25 000 units potassium penicillin and 25 000 μg streptomycin sulfate and 200mM L Glutamin 25 × 4 5 mL
    Catalog Number:
    17-718r
    Price:
    None
    Category:
    Culture Media and Reagents
    Buy from Supplier


    Structured Review

    Lonza penicillin streptomycin
    Pen Strep L Glutamin mixture contains 25 000 units potassium penicillin and 25 000 μg streptomycin sulfate and 200mM L Glutamin 25 × 4 5 mL
    https://www.bioz.com/result/penicillin streptomycin/product/Lonza
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    penicillin streptomycin - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Improvement of insulin resistance by Cyanidin 3-glucoside, anthocyanin from black beans through the up-regulation of GLUT4 gene expression
    Article Snippet: The purpose of the present study was to determine the effects of Cy-3-G on GLUT4 gene expression to improve insulin resistance in 3T3-L1 adipocytes. .. Cell culture and treatment Adipocytes, 3T3-L1 cells (Riken Cell Bank, Ibaraki, Japan), were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma Chemical Co, St. Louis, MO, USA) supplemented with 10 % fetal bovine serum (FBS) (Biowest, Miami, FL, USA, Japan) and 1% 5,000 units/ml penicillin, 5,000 μg/mL streptomycin (PS) (Lonza Walkersville, MD,USA) and incubated in 37 °C in 5 % CO2 . .. For adipocyte differentiation, 3T3-L1 cells were cultured at 3.0×105 cells/well in 6-well plates.

    Article Title: Nrf2 Activator PB125® as a Potential Therapeutic Agent Against COVID-19
    Article Snippet: .. The cells were cultured and maintained by standard methods, using Opti-MEM medium with 4% fetal bovine serum (FBS) and geneticin/penicillin/streptomycin for HepG2 cells, and using EGM2-MV medium for HPAEC cells, from Lonza (Morristown, NJ). .. HPAEC subculturing was limited to six passages in order to prevent senescence and de-differentiation.

    Article Title: Novel triazoles of 3-acetylbetulin and betulone as anticancer agents
    Article Snippet: .. All cancer cell lines were cultured in DMEM (Lonza, Basel, Switzerland) growth medium containing 10% fetal bovine serum (FBS) (Biological Industries, Cromwell, USA), penicillin (10,000 U/mL) and streptomycin(10 mg/mL) (Lonza, Basel, Switzerland). .. A WST-1 assay (Roche Diagnostics GmbH, Mannheim,Germany) was used for the evaluate of cytotoxicity against the tested human cancer cell lines.

    Modification:

    Article Title: Improvement of insulin resistance by Cyanidin 3-glucoside, anthocyanin from black beans through the up-regulation of GLUT4 gene expression
    Article Snippet: The purpose of the present study was to determine the effects of Cy-3-G on GLUT4 gene expression to improve insulin resistance in 3T3-L1 adipocytes. .. Cell culture and treatment Adipocytes, 3T3-L1 cells (Riken Cell Bank, Ibaraki, Japan), were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma Chemical Co, St. Louis, MO, USA) supplemented with 10 % fetal bovine serum (FBS) (Biowest, Miami, FL, USA, Japan) and 1% 5,000 units/ml penicillin, 5,000 μg/mL streptomycin (PS) (Lonza Walkersville, MD,USA) and incubated in 37 °C in 5 % CO2 . .. For adipocyte differentiation, 3T3-L1 cells were cultured at 3.0×105 cells/well in 6-well plates.

    Article Title: Low physiologic oxygen tensions reduce proliferation and differentiation of human multipotent mesenchymal stromal cells
    Article Snippet: Briefly, bone marrow samples (0.5 ml) were heparinized, subjected to red blood cell lysis using the ammonium chloride method, and washed with Hank's buffered salt solution (Lonza, Basel, Switzerland). .. Cells were placed in low glucose Dulbecco's Modified Eagle Medium (LG-DMEM, Lonza) supplemented with 5% (v/v) human fresh frozen plasma (FFP), 107 /ml platelets (University of Tübingen blood donor centre), 80 IU/ml heparin sulphate, 1 mM glutamine (Lonza), 100 IU/ml penicillin, 100 μg/ml streptomycin (both Biochrom, Berlin, Germany) in one well of a six-well culture plate. .. At 80% confluence, cells were harvested with trypsin 0.5% (Lonza) and replated in the above medium at 2,000 cells/cm2 .

    Incubation:

    Article Title: Improvement of insulin resistance by Cyanidin 3-glucoside, anthocyanin from black beans through the up-regulation of GLUT4 gene expression
    Article Snippet: The purpose of the present study was to determine the effects of Cy-3-G on GLUT4 gene expression to improve insulin resistance in 3T3-L1 adipocytes. .. Cell culture and treatment Adipocytes, 3T3-L1 cells (Riken Cell Bank, Ibaraki, Japan), were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma Chemical Co, St. Louis, MO, USA) supplemented with 10 % fetal bovine serum (FBS) (Biowest, Miami, FL, USA, Japan) and 1% 5,000 units/ml penicillin, 5,000 μg/mL streptomycin (PS) (Lonza Walkersville, MD,USA) and incubated in 37 °C in 5 % CO2 . .. For adipocyte differentiation, 3T3-L1 cells were cultured at 3.0×105 cells/well in 6-well plates.

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  • 94
    Lonza hek blue tlr5 heat inactivated fetal calf serum
    Presence and immune modulation effect of E. coli flagellin in B. subtilis cell wall fractions. A. E. coli flagellin in cell wall fractions of B. subtilis expressing the cell wall attached (lane 1) or the secreted (lane 2) form of flagellin, and a control expressing no flagellin (lane 3); Arrow points to the cell wall attached flagellin at the expected position of approximately 63 kDa. B. NF-κB activation capacity of washed B. subtilis VI7692 bacteria expressing a secreted (sec) or cell wall attached (cwa) form of E. coli flagellin. Grey bars, bacteria in which flagellin (if present) had been induced with IPTG; open bars, bacteria in which flagellin had not been induced. Bars indicate the relative activity of an SEAP reporter gene under the control of an NF-κB dependent promoter in <t>HEK-Blue</t> <t>TLR5</t> cells, in vitro . -, HEK-Blue TLR cells only; *, S. typhimurium flagellin (10 ng/mL); pDG, presence (+) or absence (−) of pDG148 (empty vector); pF-EC(sec), presence or absence of pDGfliC-SEC encoding a secreted form of E. coli flagellin; pF-EC(cwa), presence or absence of pDGfliC-CWA encoding a cell wall attached form of E. coli flagellin. One representative experiment is shown out of two repetitions each performed in triplicate.
    Hek Blue Tlr5 Heat Inactivated Fetal Calf Serum, supplied by Lonza, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek blue tlr5 heat inactivated fetal calf serum/product/Lonza
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek blue tlr5 heat inactivated fetal calf serum - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    98
    Lonza egm 2 singlequots
    Vasculogenesis in gelPEG and Matrigel characterized by stabilized capillary-like structures with lumen. Human GFP-ECFCs and MSCs were cocultured for 10 d in <t>EGM-2.</t> a,e) Projections of 150 μm confocal stacks through a hydrogel. b,f) Stabilization of vascular networks by pericyte-like cells (red). c,g) Capillary-like structures were composed of multiple fused endothelial cells indicated by cell-cell contact by VE-cadherin junctions. d,h) Capillary-like structures in gelPEG and Matrigel were characterized by lumenization (dotted lines indicate cutting sections). i) Capillary-like structures with flopodia at a site of active angiogenesis, here in a gelPEG hydrogel. j,k) Total vessel length and average vessel thickness in gelPEG hydrogels and Matrigel. Data are depicted as mean + SD; N = 3, n = 3.
    Egm 2 Singlequots, supplied by Lonza, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egm 2 singlequots/product/Lonza
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egm 2 singlequots - by Bioz Stars, 2021-03
    98/100 stars
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    95
    Lonza amphotericin b hek293 cells
    IKK overexpression overcomes reovirus mediated blockade of NF-κB signaling. <t>(A)</t> HEK293 cells were adsorbed with PBS (mock) or 10 PFU/cell of T3A. Following incubation at 37°C for 24 h, cells were treated with 10 ng/ml TNFα and incubated for 1 h. RNA was extracted from cells and IκBα gene expression was measured using RT-qPCR. Gene expression in mock infected cells treated with TNFα was set to 100%. Gene expression of each replicate, the mean value and SD are shown. *, P
    Amphotericin B Hek293 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amphotericin b hek293 cells/product/Lonza
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amphotericin b hek293 cells - by Bioz Stars, 2021-03
    95/100 stars
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    Image Search Results


    Presence and immune modulation effect of E. coli flagellin in B. subtilis cell wall fractions. A. E. coli flagellin in cell wall fractions of B. subtilis expressing the cell wall attached (lane 1) or the secreted (lane 2) form of flagellin, and a control expressing no flagellin (lane 3); Arrow points to the cell wall attached flagellin at the expected position of approximately 63 kDa. B. NF-κB activation capacity of washed B. subtilis VI7692 bacteria expressing a secreted (sec) or cell wall attached (cwa) form of E. coli flagellin. Grey bars, bacteria in which flagellin (if present) had been induced with IPTG; open bars, bacteria in which flagellin had not been induced. Bars indicate the relative activity of an SEAP reporter gene under the control of an NF-κB dependent promoter in HEK-Blue TLR5 cells, in vitro . -, HEK-Blue TLR cells only; *, S. typhimurium flagellin (10 ng/mL); pDG, presence (+) or absence (−) of pDG148 (empty vector); pF-EC(sec), presence or absence of pDGfliC-SEC encoding a secreted form of E. coli flagellin; pF-EC(cwa), presence or absence of pDGfliC-CWA encoding a cell wall attached form of E. coli flagellin. One representative experiment is shown out of two repetitions each performed in triplicate.

    Journal: PLoS ONE

    Article Title: High-Throughput System for the Presentation of Secreted and Surface-Exposed Proteins from Gram-Positive Bacteria in Functional Metagenomics Studies

    doi: 10.1371/journal.pone.0065956

    Figure Lengend Snippet: Presence and immune modulation effect of E. coli flagellin in B. subtilis cell wall fractions. A. E. coli flagellin in cell wall fractions of B. subtilis expressing the cell wall attached (lane 1) or the secreted (lane 2) form of flagellin, and a control expressing no flagellin (lane 3); Arrow points to the cell wall attached flagellin at the expected position of approximately 63 kDa. B. NF-κB activation capacity of washed B. subtilis VI7692 bacteria expressing a secreted (sec) or cell wall attached (cwa) form of E. coli flagellin. Grey bars, bacteria in which flagellin (if present) had been induced with IPTG; open bars, bacteria in which flagellin had not been induced. Bars indicate the relative activity of an SEAP reporter gene under the control of an NF-κB dependent promoter in HEK-Blue TLR5 cells, in vitro . -, HEK-Blue TLR cells only; *, S. typhimurium flagellin (10 ng/mL); pDG, presence (+) or absence (−) of pDG148 (empty vector); pF-EC(sec), presence or absence of pDGfliC-SEC encoding a secreted form of E. coli flagellin; pF-EC(cwa), presence or absence of pDGfliC-CWA encoding a cell wall attached form of E. coli flagellin. One representative experiment is shown out of two repetitions each performed in triplicate.

    Article Snippet: Both culture media were supplemented with 2 mM L-glutamine, 50 IU/mL penicillin, 50 µg/mL streptomycin and 10% (or 20% for HEK-Blue TLR5) heat-inactivated fetal calf serum (FCS, Lonza).

    Techniques: Expressing, Activation Assay, Size-exclusion Chromatography, Activity Assay, In Vitro, Plasmid Preparation

    Vasculogenesis in gelPEG and Matrigel characterized by stabilized capillary-like structures with lumen. Human GFP-ECFCs and MSCs were cocultured for 10 d in EGM-2. a,e) Projections of 150 μm confocal stacks through a hydrogel. b,f) Stabilization of vascular networks by pericyte-like cells (red). c,g) Capillary-like structures were composed of multiple fused endothelial cells indicated by cell-cell contact by VE-cadherin junctions. d,h) Capillary-like structures in gelPEG and Matrigel were characterized by lumenization (dotted lines indicate cutting sections). i) Capillary-like structures with flopodia at a site of active angiogenesis, here in a gelPEG hydrogel. j,k) Total vessel length and average vessel thickness in gelPEG hydrogels and Matrigel. Data are depicted as mean + SD; N = 3, n = 3.

    Journal: Advanced healthcare materials

    Article Title: A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues

    doi: 10.1002/adhm.201900979

    Figure Lengend Snippet: Vasculogenesis in gelPEG and Matrigel characterized by stabilized capillary-like structures with lumen. Human GFP-ECFCs and MSCs were cocultured for 10 d in EGM-2. a,e) Projections of 150 μm confocal stacks through a hydrogel. b,f) Stabilization of vascular networks by pericyte-like cells (red). c,g) Capillary-like structures were composed of multiple fused endothelial cells indicated by cell-cell contact by VE-cadherin junctions. d,h) Capillary-like structures in gelPEG and Matrigel were characterized by lumenization (dotted lines indicate cutting sections). i) Capillary-like structures with flopodia at a site of active angiogenesis, here in a gelPEG hydrogel. j,k) Total vessel length and average vessel thickness in gelPEG hydrogels and Matrigel. Data are depicted as mean + SD; N = 3, n = 3.

    Article Snippet: Endothelial growth medium-2 (EGM-2) was composed of endothelial basal medium-2 (EBM, Lonza), 10% v/v FBS, 100 U mL−1 penicillin, 10 mg mL−1 streptomycin and EGM-2 singlequots (Lonza).

    Techniques:

    IKK overexpression overcomes reovirus mediated blockade of NF-κB signaling. (A) HEK293 cells were adsorbed with PBS (mock) or 10 PFU/cell of T3A. Following incubation at 37°C for 24 h, cells were treated with 10 ng/ml TNFα and incubated for 1 h. RNA was extracted from cells and IκBα gene expression was measured using RT-qPCR. Gene expression in mock infected cells treated with TNFα was set to 100%. Gene expression of each replicate, the mean value and SD are shown. *, P

    Journal: bioRxiv

    Article Title: Loss of IKK subunits limits NF-κB signaling in reovirus infected cells

    doi: 10.1101/843680

    Figure Lengend Snippet: IKK overexpression overcomes reovirus mediated blockade of NF-κB signaling. (A) HEK293 cells were adsorbed with PBS (mock) or 10 PFU/cell of T3A. Following incubation at 37°C for 24 h, cells were treated with 10 ng/ml TNFα and incubated for 1 h. RNA was extracted from cells and IκBα gene expression was measured using RT-qPCR. Gene expression in mock infected cells treated with TNFα was set to 100%. Gene expression of each replicate, the mean value and SD are shown. *, P

    Article Snippet: Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS, 2 mM L-glutamine, 100 U/ml of penicillin, 100 μg/ml of streptomycin, and 25 ng/ml of amphotericin B. HEK293 cells (obtained from M. Marketon’s laboratory) were maintained in Dulbecco’s modified essential medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamine.

    Techniques: Over Expression, Incubation, Expressing, Quantitative RT-PCR, Infection