pe conjugated mouse anti human vegfr 3  (R&D Systems)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Name:
    Human VEGF R3 Flt 4 PE conjugated Antibody
    Description:
    The Human VEGF R3 Flt 4 PE conjugated Antibody from R D Systems is a mouse monoclonal antibody to VEGF R3 Flt 4 This antibody reacts with human The Human VEGF R3 Flt 4 PE conjugated Antibody has been validated for the following applications Flow Cytometry
    Catalog Number:
    FAB3492P
    Price:
    319
    Category:
    Primary Antibodies
    Applications:
    Flow Cytometry
    Purity:
    Protein A or G purified from hybridoma culture supernatant
    Conjugate:
    Phycoerythrin
    Immunogen:
    Mouse myeloma cell line NS0-derived recombinant human VEGF R3/Flt-4, Tyr25-Ile776, Accession # P35916
    Size:
    100 Tests
    Host:
    Mouse
    Isotype:
    IgG1
    Buy from Supplier


    Structured Review

    R&D Systems pe conjugated mouse anti human vegfr 3
    Human VEGF R3 Flt 4 PE conjugated Antibody
    The Human VEGF R3 Flt 4 PE conjugated Antibody from R D Systems is a mouse monoclonal antibody to VEGF R3 Flt 4 This antibody reacts with human The Human VEGF R3 Flt 4 PE conjugated Antibody has been validated for the following applications Flow Cytometry
    https://www.bioz.com/result/pe conjugated mouse anti human vegfr 3/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe conjugated mouse anti human vegfr 3 - by Bioz Stars, 2021-04
    90/100 stars

    Images

    1) Product Images from "NFATc1 regulates lymphatic endothelial development"

    Article Title: NFATc1 regulates lymphatic endothelial development

    Journal: Mechanisms of development

    doi: 10.1016/j.mod.2009.02.003

    NFATc1 is coexpressed with Prox-1 and VEGFR-3 on developing and mature lymphatic endothelial cells
    Figure Legend Snippet: NFATc1 is coexpressed with Prox-1 and VEGFR-3 on developing and mature lymphatic endothelial cells

    Techniques Used:

    Related Articles

    Staining:

    Article Title: Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair
    Article Snippet: Flow cytometry FACS staining and analysis were performed as previously described . .. For four-marker staining, cells were incubated with Alexa488-conjugated rat anti-human PODOPLANIN (Biolegend), PE-conjugated mouse anti-human VEGFR3 (R & D system), and biotinylated rabbit anti-human LYVE-1 (ReliaTech GmbH) followed by streptavidin PE-Cy5 (BD Pharminogen). .. After washing, the cells were fixed/permeabilized (IC Fixation/Permeabilization buffer, eBioscience) and incubated with goat anti-human PROX-1 (R & D Systems), followed by donkey APC-Cy7-conjugated anti-goat IgG (Santa cruz).

    Incubation:

    Article Title: Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair
    Article Snippet: Flow cytometry FACS staining and analysis were performed as previously described . .. For four-marker staining, cells were incubated with Alexa488-conjugated rat anti-human PODOPLANIN (Biolegend), PE-conjugated mouse anti-human VEGFR3 (R & D system), and biotinylated rabbit anti-human LYVE-1 (ReliaTech GmbH) followed by streptavidin PE-Cy5 (BD Pharminogen). .. After washing, the cells were fixed/permeabilized (IC Fixation/Permeabilization buffer, eBioscience) and incubated with goat anti-human PROX-1 (R & D Systems), followed by donkey APC-Cy7-conjugated anti-goat IgG (Santa cruz).

    Article Title: Vascular endothelial growth factor can signal through platelet-derived growth factor receptors
    Article Snippet: An additional pair of primers for all three VEGFRs (VEGFR1-3) that was designed to different sequence regions were also used (primer sequences not shown). .. Flow cytometry For single-color flow cytometry, MSCs, HUVECs, or HDFs (4×106 cells/ml) were incubated with either PE-conjugated anti–human VEGFR1-PE (FAB321P), VEGFR2-PE (FAB357P), or VEGFR3-PE (FAB3492P) antibodies, or control anti–IgG1 -PE antibody (IC002P; R & D Systems). .. VEGFR1 (MAB4711), VEGFR2 (MAB3572), VEGFR3 (MAB3491) antibodies, or control anti-IgG1 (MAB002) antibody (R & D Systems) were also used after secondary labeling with a FITC secondary antibody (Dako Cytomation).

    Article Title: VEGFR3 Inhibition Chemosensitizes Ovarian Cancer Stemlike Cells through Down-Regulation of BRCA1 and BRCA2
    Article Snippet: .. Cells were incubated with monoclonal anti-human VEGF R3-PE (R & D Systems, Minneapolis, MN) and CD133/2 (Allophycocyanin [APC]-tagged; Miltenyi Biotec, Gladbach, Germany) or for isotype control with Mouse IgG2A APC and Mouse IgG2A phycoerythrin (PE) (R & D Systems) and then ALDEFLUOR (Stemcell Technologies, Vancouver, Canada) as previously described . .. Results were analyzed using Summit 6.0 (Beckman-Coulter, La Brea, CA).

    Article Title: NFATc1 regulates lymphatic endothelial development
    Article Snippet: For activation studies, HMVEC-L were cultured overnight in EGM-2MV media with 1% serum and without supplemental VEGF-A and FGF (EGM-1%). .. For flow cytometry, cells were lifted with 0.02% EDTA (Gibco), washed with phosphate-buffered saline (PBS), blocked and incubated with PE-conjugated mouse anti-human VEGFR-3 (R & D Systems), mouse anti-human podoplanin (Fitzgerald Industries) or isotype controls for 1 hour. .. Cells were washed in PBS and incubated with goat anti-mouse Alexa 647 secondary antibody for podoplanin (Molecular Probes) for 30 min.

    Flow Cytometry:

    Article Title: Vascular endothelial growth factor can signal through platelet-derived growth factor receptors
    Article Snippet: An additional pair of primers for all three VEGFRs (VEGFR1-3) that was designed to different sequence regions were also used (primer sequences not shown). .. Flow cytometry For single-color flow cytometry, MSCs, HUVECs, or HDFs (4×106 cells/ml) were incubated with either PE-conjugated anti–human VEGFR1-PE (FAB321P), VEGFR2-PE (FAB357P), or VEGFR3-PE (FAB3492P) antibodies, or control anti–IgG1 -PE antibody (IC002P; R & D Systems). .. VEGFR1 (MAB4711), VEGFR2 (MAB3572), VEGFR3 (MAB3491) antibodies, or control anti-IgG1 (MAB002) antibody (R & D Systems) were also used after secondary labeling with a FITC secondary antibody (Dako Cytomation).

    Article Title: NFATc1 regulates lymphatic endothelial development
    Article Snippet: For activation studies, HMVEC-L were cultured overnight in EGM-2MV media with 1% serum and without supplemental VEGF-A and FGF (EGM-1%). .. For flow cytometry, cells were lifted with 0.02% EDTA (Gibco), washed with phosphate-buffered saline (PBS), blocked and incubated with PE-conjugated mouse anti-human VEGFR-3 (R & D Systems), mouse anti-human podoplanin (Fitzgerald Industries) or isotype controls for 1 hour. .. Cells were washed in PBS and incubated with goat anti-mouse Alexa 647 secondary antibody for podoplanin (Molecular Probes) for 30 min.

    Cytometry:

    Article Title: Vascular endothelial growth factor can signal through platelet-derived growth factor receptors
    Article Snippet: An additional pair of primers for all three VEGFRs (VEGFR1-3) that was designed to different sequence regions were also used (primer sequences not shown). .. Flow cytometry For single-color flow cytometry, MSCs, HUVECs, or HDFs (4×106 cells/ml) were incubated with either PE-conjugated anti–human VEGFR1-PE (FAB321P), VEGFR2-PE (FAB357P), or VEGFR3-PE (FAB3492P) antibodies, or control anti–IgG1 -PE antibody (IC002P; R & D Systems). .. VEGFR1 (MAB4711), VEGFR2 (MAB3572), VEGFR3 (MAB3491) antibodies, or control anti-IgG1 (MAB002) antibody (R & D Systems) were also used after secondary labeling with a FITC secondary antibody (Dako Cytomation).

    Article Title: NFATc1 regulates lymphatic endothelial development
    Article Snippet: For activation studies, HMVEC-L were cultured overnight in EGM-2MV media with 1% serum and without supplemental VEGF-A and FGF (EGM-1%). .. For flow cytometry, cells were lifted with 0.02% EDTA (Gibco), washed with phosphate-buffered saline (PBS), blocked and incubated with PE-conjugated mouse anti-human VEGFR-3 (R & D Systems), mouse anti-human podoplanin (Fitzgerald Industries) or isotype controls for 1 hour. .. Cells were washed in PBS and incubated with goat anti-mouse Alexa 647 secondary antibody for podoplanin (Molecular Probes) for 30 min.

    other:

    Article Title: Morphological and Phenotypic Analyses of the Human Placenta Using Whole Mount Immunofluorescence 1
    Article Snippet: The following primary antibodies were used: anti-human CD31 (1:10 dilution for PE-conjugate and 1:40 for APC-conjugate; clone WM59, BioLegend, San Diego, CA), anti-human CD45 (1:10 dilution; clone H130, BioLegend), anti-human CD68 (1:10; clone Y1/82A, BioLegend), anti-human chorionic gonadotropin (hCG) (1:10; clone A0231, Dako, Glostrup, Denmark), anti-human cytokeratin 7 (1:40; clone LP5K, Millipore, Billerica, MA), anti-human HLA-G (1:100; clone MEM-G/11; Thermo Scientific, Waltham, MA), anti-human VEGFR1 (1:66; clone Y103, Abcam, Cambridge, England), anti-human VEGFR2 (1:10; clone 89106, BD Biosciences, San Jose, CA), anti-human VEGFR3 (1:10; clone 54733, R & D Systems, Minneapolis, MN), and anti-human STAT3 (pY705) (1:40; clone 4/P-STAT3, BD Biosciences).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    R&D Systems pe conjugated mouse anti human vegfr3
    Immunocytochemistry showing expression of LEC markers in the differentiated hESCs and hiPSCs. The pluripotent stem cells differentiated in suspension culture were subsequently cultured for 10 to 15 days under the co-culture conditions. ( a ) Single LEC marker staining for LYVE-1, <t>VEGFR3,</t> or PODOPLANIN (POD). DAPI: blue. ( b-d ) Double LEC mar ker staining LYVE-1 and PROX-1 ( b ), VEGFR-3 and PODOPLANIN ( c ), and PODOPLANIN and LYVE-1 ( d ) in H9-derived LECs. ( e ) Triple LEC marker staining LYVE-1, PODOPLANIIN, PROX-1 in H9-derived LECs. Arrows indicate the representative cells stained positive for two or three LEC markers as labeled.
    Pe Conjugated Mouse Anti Human Vegfr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe conjugated mouse anti human vegfr3/product/R&D Systems
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe conjugated mouse anti human vegfr3 - by Bioz Stars, 2021-04
    90/100 stars
      Buy from Supplier

    Image Search Results


    Immunocytochemistry showing expression of LEC markers in the differentiated hESCs and hiPSCs. The pluripotent stem cells differentiated in suspension culture were subsequently cultured for 10 to 15 days under the co-culture conditions. ( a ) Single LEC marker staining for LYVE-1, VEGFR3, or PODOPLANIN (POD). DAPI: blue. ( b-d ) Double LEC mar ker staining LYVE-1 and PROX-1 ( b ), VEGFR-3 and PODOPLANIN ( c ), and PODOPLANIN and LYVE-1 ( d ) in H9-derived LECs. ( e ) Triple LEC marker staining LYVE-1, PODOPLANIIN, PROX-1 in H9-derived LECs. Arrows indicate the representative cells stained positive for two or three LEC markers as labeled.

    Journal: Scientific Reports

    Article Title: Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair

    doi: 10.1038/srep11019

    Figure Lengend Snippet: Immunocytochemistry showing expression of LEC markers in the differentiated hESCs and hiPSCs. The pluripotent stem cells differentiated in suspension culture were subsequently cultured for 10 to 15 days under the co-culture conditions. ( a ) Single LEC marker staining for LYVE-1, VEGFR3, or PODOPLANIN (POD). DAPI: blue. ( b-d ) Double LEC mar ker staining LYVE-1 and PROX-1 ( b ), VEGFR-3 and PODOPLANIN ( c ), and PODOPLANIN and LYVE-1 ( d ) in H9-derived LECs. ( e ) Triple LEC marker staining LYVE-1, PODOPLANIIN, PROX-1 in H9-derived LECs. Arrows indicate the representative cells stained positive for two or three LEC markers as labeled.

    Article Snippet: For four-marker staining, cells were incubated with Alexa488-conjugated rat anti-human PODOPLANIN (Biolegend), PE-conjugated mouse anti-human VEGFR3 (R & D system), and biotinylated rabbit anti-human LYVE-1 (ReliaTech GmbH) followed by streptavidin PE-Cy5 (BD Pharminogen).

    Techniques: Immunocytochemistry, Expressing, Cell Culture, Co-Culture Assay, Marker, Staining, Derivative Assay, Labeling

    Contribution of LYVE-1 + PODOPLANIN + cells derived from hESCs and hiPSCs to new lymphatic vessel formation. ( a ) In vitro tube formation assay. DiI-labeled LYVE-1 + PODOPLANIN + cells (red) derived from hiESC (H1) cocultured with hLECs onto Matrigel for 12 hours, showing their incorporation into vascular structures consisting of hLECs. ( b ) DiI-labeled LYVE-1 + PODOPLANIN + cells which were differentiated from H1 and BJ1 cells were injected into mice in an ear wound model. Two weeks later, tissues were harvested and subjected to immunohistochemistry with VEGFR3, PODOPLANIN, and LYVE-1 antibodies on frozen tissue sections. Confocal microscopic imaging revealed incorporation of injected cells (red) into lymphatic vascular structures and expression of PODOPLANIN and VEGFR3. ( c ) mRNA expression of the indicated lymphangiogenic genes in pre-sorted cells at day 14, LYVE1 + PODOPLANIN + cells (BJ1-derived), LYVE1 − PODOPLANIN − cells (BJ1-derived), and hLECs measured by qRT-PCR. Data were presented as relative mRNA expression to GAPDH. N = 3 to 4 per group. * P

    Journal: Scientific Reports

    Article Title: Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair

    doi: 10.1038/srep11019

    Figure Lengend Snippet: Contribution of LYVE-1 + PODOPLANIN + cells derived from hESCs and hiPSCs to new lymphatic vessel formation. ( a ) In vitro tube formation assay. DiI-labeled LYVE-1 + PODOPLANIN + cells (red) derived from hiESC (H1) cocultured with hLECs onto Matrigel for 12 hours, showing their incorporation into vascular structures consisting of hLECs. ( b ) DiI-labeled LYVE-1 + PODOPLANIN + cells which were differentiated from H1 and BJ1 cells were injected into mice in an ear wound model. Two weeks later, tissues were harvested and subjected to immunohistochemistry with VEGFR3, PODOPLANIN, and LYVE-1 antibodies on frozen tissue sections. Confocal microscopic imaging revealed incorporation of injected cells (red) into lymphatic vascular structures and expression of PODOPLANIN and VEGFR3. ( c ) mRNA expression of the indicated lymphangiogenic genes in pre-sorted cells at day 14, LYVE1 + PODOPLANIN + cells (BJ1-derived), LYVE1 − PODOPLANIN − cells (BJ1-derived), and hLECs measured by qRT-PCR. Data were presented as relative mRNA expression to GAPDH. N = 3 to 4 per group. * P

    Article Snippet: For four-marker staining, cells were incubated with Alexa488-conjugated rat anti-human PODOPLANIN (Biolegend), PE-conjugated mouse anti-human VEGFR3 (R & D system), and biotinylated rabbit anti-human LYVE-1 (ReliaTech GmbH) followed by streptavidin PE-Cy5 (BD Pharminogen).

    Techniques: Derivative Assay, In Vitro, Tube Formation Assay, Labeling, Injection, Mouse Assay, Immunohistochemistry, Imaging, Expressing, Quantitative RT-PCR

    NFATc1 is coexpressed with Prox-1 and VEGFR-3 on developing and mature lymphatic endothelial cells

    Journal: Mechanisms of development

    Article Title: NFATc1 regulates lymphatic endothelial development

    doi: 10.1016/j.mod.2009.02.003

    Figure Lengend Snippet: NFATc1 is coexpressed with Prox-1 and VEGFR-3 on developing and mature lymphatic endothelial cells

    Article Snippet: For flow cytometry, cells were lifted with 0.02% EDTA (Gibco), washed with phosphate-buffered saline (PBS), blocked and incubated with PE-conjugated mouse anti-human VEGFR-3 (R & D Systems), mouse anti-human podoplanin (Fitzgerald Industries) or isotype controls for 1 hour.

    Techniques: