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Illumina Inc pe adapter oligonucleotides
Pe Adapter Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pe adapter oligonucleotides/product/Illumina Inc
Average 87 stars, based on 4 article reviews
Price from $9.99 to $1999.99
pe adapter oligonucleotides - by Bioz Stars, 2020-01
87/100 stars

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RNA Extraction:

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: Paragraph title: RNA extraction and sequencing ... Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments.

Amplification:

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments. .. These were PCR amplified through 15 cycles to prior to sequencing using an Illumina HiSeq 2000 with 90 bp, paired-end reads.

RNA Sequencing Assay:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: Paragraph title: 2.3. cDNA Library Construction and RNA-Seq ... After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

Magnetic Beads:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: The mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Cat. No. MRZ11124C, Illumina Inc. CA, USA). .. After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: Total RNA was delivered to the service provider, BGI (Shenzen, China), where each biological replicate was sequenced separately. oligodT coupled magnetic beads were used to isolate poly-A+ mRNA, which was used as a template for cDNA synthesis (Superscript II, Invitrogen) primed by random hexamers, followed by second strand synthesis using E. coli DNA PolI (Invitrogen). .. Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments.

Synthesized:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: The first strand cDNA was synthesized using random oligonucleotide primers and SuperScriptTM II reverse transcriptase (Cat. No. 18064014, Invitrogen, CA, USA), while the second strand cDNA synthesis was undertaken using DNA polymerase I. .. After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

Purification:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: The mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Cat. No. MRZ11124C, Illumina Inc. CA, USA). .. After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: .. Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments. .. These were PCR amplified through 15 cycles to prior to sequencing using an Illumina HiSeq 2000 with 90 bp, paired-end reads.

Real-time Polymerase Chain Reaction:

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments. .. The quality of the sample during processing prior to sequencing was monitored using the Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System.

Polymerase Chain Reaction:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization. .. DNA fragments with ligated adaptor molecules on both ends were selectively enriched using an Illumina PCR primer cocktail in a 15-cycle PCR reaction.

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: .. Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments. .. These were PCR amplified through 15 cycles to prior to sequencing using an Illumina HiSeq 2000 with 90 bp, paired-end reads.

cDNA Library Assay:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: Paragraph title: 2.3. cDNA Library Construction and RNA-Seq ... After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.

Sequencing:

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: Paragraph title: RNA extraction and sequencing ... Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments.

Hybridization:

Article Title: Transcriptome Profile Analysis of Mammary Gland Tissue from Two Breeds of Lactating Sheep
Article Snippet: .. After adenylation of the 3′-ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization. .. To select cDNA fragments of the preferred ~200 bp in length, the library fragments were purified using the AMPure XP system (Cat. No. A63880, Beckman Coulter, CA, USA).

Chromatin Immunoprecipitation:

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: Extracted RNA was then digested with TURBO DNA-free™ Kit (Life Technologies) to remove DNA contamination and their quality was evaluated using a RNA 6000 Nano chip (Agilent Technologies) on an Agilent 2100 Bioanalyzer. .. Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments.

Gel Purification:

Article Title: RNASeq Analysis of the Shoot Apex of Flax (Linum usitatissimum) to Identify Phloem Fiber Specification Genes
Article Snippet: .. Double-stranded cDNA (Qiaquick PCR Purification Kit, Qiagen), was sheared with a nebulizer, end repaired, and ligated to Illumina PE adapter oligos, and the products size-selected by gel purification to produce 200 bp fragments. .. These were PCR amplified through 15 cycles to prior to sequencing using an Illumina HiSeq 2000 with 90 bp, paired-end reads.

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    Illumina Inc index pe adaptor oligo mix
    Index Pe Adaptor Oligo Mix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/index pe adaptor oligo mix/product/Illumina Inc
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    index pe adaptor oligo mix - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    79
    Illumina Inc pe adaptor oligo mix
    Pe Adaptor Oligo Mix, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe adaptor oligo mix/product/Illumina Inc
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pe adaptor oligo mix - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

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