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Illumina Inc rnase
Rnase, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 1270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase/product/Illumina Inc
Average 93 stars, based on 1270 article reviews
Price from $9.99 to $1999.99
rnase - by Bioz Stars, 2020-07
93/100 stars

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Amplification:

Article Title: Transcriptome Analysis Provides Insight into the Molecular Mechanisms Underlying gametophyte factor 2-Mediated Cross-Incompatibility in Maize
Article Snippet: .. The mRNA was isolated from the total RNA for fragmentation, cDNA synthesis, and PCR amplification, to generate the cDNA libraries following the Illumina RNA Sequencing Protocol. .. The quality of RNA extraction was assessed by ND-1000 spectroscopy (Thermo Scientific, Waltham, MA, USA) with concentration greater than 60 ng/μL and OD260/280 between 1.8 and 2.2, and OD260/230 greater than 2.0.

RNA Sequencing Assay:

Article Title: Transcriptome Analysis Provides Insight into the Molecular Mechanisms Underlying gametophyte factor 2-Mediated Cross-Incompatibility in Maize
Article Snippet: .. The mRNA was isolated from the total RNA for fragmentation, cDNA synthesis, and PCR amplification, to generate the cDNA libraries following the Illumina RNA Sequencing Protocol. .. The quality of RNA extraction was assessed by ND-1000 spectroscopy (Thermo Scientific, Waltham, MA, USA) with concentration greater than 60 ng/μL and OD260/280 between 1.8 and 2.2, and OD260/230 greater than 2.0.

Isolation:

Article Title: Transcriptome Analysis Provides Insight into the Molecular Mechanisms Underlying gametophyte factor 2-Mediated Cross-Incompatibility in Maize
Article Snippet: .. The mRNA was isolated from the total RNA for fragmentation, cDNA synthesis, and PCR amplification, to generate the cDNA libraries following the Illumina RNA Sequencing Protocol. .. The quality of RNA extraction was assessed by ND-1000 spectroscopy (Thermo Scientific, Waltham, MA, USA) with concentration greater than 60 ng/μL and OD260/280 between 1.8 and 2.2, and OD260/230 greater than 2.0.

Construct:

Article Title: Coordinate regulation of long non-coding RNAs and protein-coding genes in germ-free mice
Article Snippet: .. The complementary DNA (cDNA) libraries were constructed from total RNA samples using a TruSeq RNA Sample Prep Kit with poly-A tail selection (Illumina, San Diego, CA). .. Sequencing was performed on an Illumina HiSeq2000 sequencer using a 50 bp paired-end multiplexing strategy at the Kansas University Medical Center Genome Sequencing Facility.

Purification:

Article Title: Combining Different mRNA Capture Methods to Analyze the Transcriptome: Analysis of the Xenopus laevis Transcriptome
Article Snippet: .. Oligo dT Purificaiton A total of 5μg of total RNA was used as input for oligo-dT purification using the Illumina tru-Seq mRNA library purification kit for the following libraries: Mitosis Total, Interphase Total, Oocyte dT, Mitosis BWR4, Mitosis Total1, Mitosis Total 2, Taxol Mitosis, SN Mitosis. .. To compare mRNA capture efficiency between cap-capture and oligo-dT mRNA was purified from 50μg of total RNA using the Exiquon LNA dT purification oligo according to the manufacturers instructions. mRNA yield from the Exiquon kit was ~3-5% of total RNA.

Sequencing:

Article Title: The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans
Article Snippet: .. Accession numbers All small RNA and mRNA Illumina sequencing data have been submitted to the NCBI’s Sequence Read Archive (SRA), and are included under project accession number SRP115391. .. Funding Information This paper was supported by the following grants: http://dx.doi.org/10.13039/100000062 National Institute of Diabetes and Digestive and Kidney Diseases DK075147 to Katherine McJunkin. http://dx.doi.org/10.13039/501100000024 Canadian Institutes of Health Research MOP-125894 to Quaid D Morris. http://dx.doi.org/10.13039/501100000024 Canadian Institutes of Health Research MOP-274660 to Julie M Claycomb. http://dx.doi.org/10.13039/501100007224 Connaught Fund to Julie M Claycomb. http://dx.doi.org/10.13039/501100000024 Canadian Institutes of Health Research CAP- 783 262134 to Julie M Claycomb. http://dx.doi.org/10.13039/501100001804 Canada Research Chairs to Julie M Claycomb. http://dx.doi.org/10.13039/501100003579 University of Toronto to Julie M Claycomb. http://dx.doi.org/10.13039/100000002 National Institutes of Health HD078253 to Zhiping Weng. http://dx.doi.org/10.13039/501100000925 National Health and Medical Research Council 0606575 to Peter R Boag.

Article Title: Gene family innovation, conservation and loss on the animal stem lineage
Article Snippet: .. Test of polyA selection to separate choanoflagellate from bacterial RNA The standard library preparation protocol for Illumina mRNA sequencing used poly-dT beads to separate polyadenylated mRNA from other types of non-polyadenylated RNA such as rRNA and tRNA. .. For choanoflagellates, the bead selection step also served to separate choanoflagellate mRNA from bacterial RNA (which is not polyadenylated).

Article Title: The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans
Article Snippet: .. Data availability All small RNA and mRNA Illumina sequencing data have been submitted to the NCBI's Sequence Read Archive (SRA), and are included under project accession number SRP115391. .. The following dataset was generated: Shikui Tu 2017 Caenorhabditis elegans Raw sequences reads NHL-2, Aug 12'17 NCBI Sequence Read Archive SRP115391

Selection:

Article Title: Gene family innovation, conservation and loss on the animal stem lineage
Article Snippet: .. Test of polyA selection to separate choanoflagellate from bacterial RNA The standard library preparation protocol for Illumina mRNA sequencing used poly-dT beads to separate polyadenylated mRNA from other types of non-polyadenylated RNA such as rRNA and tRNA. .. For choanoflagellates, the bead selection step also served to separate choanoflagellate mRNA from bacterial RNA (which is not polyadenylated).

Article Title: Coordinate regulation of long non-coding RNAs and protein-coding genes in germ-free mice
Article Snippet: .. The complementary DNA (cDNA) libraries were constructed from total RNA samples using a TruSeq RNA Sample Prep Kit with poly-A tail selection (Illumina, San Diego, CA). .. Sequencing was performed on an Illumina HiSeq2000 sequencer using a 50 bp paired-end multiplexing strategy at the Kansas University Medical Center Genome Sequencing Facility.

Polymerase Chain Reaction:

Article Title: Transcriptome Analysis Provides Insight into the Molecular Mechanisms Underlying gametophyte factor 2-Mediated Cross-Incompatibility in Maize
Article Snippet: .. The mRNA was isolated from the total RNA for fragmentation, cDNA synthesis, and PCR amplification, to generate the cDNA libraries following the Illumina RNA Sequencing Protocol. .. The quality of RNA extraction was assessed by ND-1000 spectroscopy (Thermo Scientific, Waltham, MA, USA) with concentration greater than 60 ng/μL and OD260/280 between 1.8 and 2.2, and OD260/230 greater than 2.0.

Sample Prep:

Article Title: Coordinate regulation of long non-coding RNAs and protein-coding genes in germ-free mice
Article Snippet: .. The complementary DNA (cDNA) libraries were constructed from total RNA samples using a TruSeq RNA Sample Prep Kit with poly-A tail selection (Illumina, San Diego, CA). .. Sequencing was performed on an Illumina HiSeq2000 sequencer using a 50 bp paired-end multiplexing strategy at the Kansas University Medical Center Genome Sequencing Facility.

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    Illumina Inc pe adaptor oligo mix
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