pcr2 1topo vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr2 1topo vector
    Pcr2 1topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr2 1topo vector/product/Thermo Fisher
    Average 96 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    pcr2 1topo vector - by Bioz Stars, 2020-08
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Analyses of Alternatively Processed Genes in Ciliates Provide Insights into the Origins of Scrambled Genomes and May Provide a Mechanism for Speciation
    Article Snippet: .. Genome walking PCR products were cloned using TA TOPO cloning kits (45-0641; Invitrogen) and screened using the polymerase TaqGold (Applied Biosystems, CA). ..

    Article Title: Robust activation of microhomology-mediated end joining for precision gene editing applications
    Article Snippet: .. Alternatively, purified amplicons were used for subcloning analysis with either Topo-TA Cloning Kit (Thermo Fisher Scientific 451641. .. Waltham, MA) or StrataClone PCR Cloning Kit (Agilent 240205.

    Article Title: Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
    Article Snippet: .. Electrophoresis was done on a 2% TAE gel and the PCR products were cloned by TOPO TA Cloning Kit (Invitrogen, Part no. 45-0641) and clones were prepared for sequencing using the GenomeLab Dye Terminator Cycle Sequencing Kit (Beckman & Coulter, P/N 608120). .. Samples were run on the CEQ8800 sequenzer (Beckman & Coulter).

    Article Title: Electrical coupling and innexin expression in the stomatogastric ganglion of the crab Cancer borealis
    Article Snippet: .. PCR products of appropriate length were cloned into pCR 2.1 TOPO cloning vector (Invitrogen, 45-0641) and verified by sequencing (Genewiz). .. For Inx1 , degenerate primers were designed by hand based on conserved protein sequence alignments from D. melanogaster , Anopheles gambiae , Bombyx mori , and Schistocerca gregaria innexins.

    Article Title: Systematic discovery of novel ciliary genes through functional genomics in the zebrafish
    Article Snippet: .. PCR bands were cloned using the TOPO-TA cloning system (Invitrogen, #450641) and sequenced. cDNA sequences were assembled using a combination of Ensembl gene predictions and EMBL ESTs as references. .. In situ hybridization The lefty2 RNA in situ probe was made by cloning a fragment of the cDNA.

    Subcloning:

    Article Title: Robust activation of microhomology-mediated end joining for precision gene editing applications
    Article Snippet: .. Alternatively, purified amplicons were used for subcloning analysis with either Topo-TA Cloning Kit (Thermo Fisher Scientific 451641. .. Waltham, MA) or StrataClone PCR Cloning Kit (Agilent 240205.

    TA Cloning:

    Article Title: Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
    Article Snippet: .. Electrophoresis was done on a 2% TAE gel and the PCR products were cloned by TOPO TA Cloning Kit (Invitrogen, Part no. 45-0641) and clones were prepared for sequencing using the GenomeLab Dye Terminator Cycle Sequencing Kit (Beckman & Coulter, P/N 608120). .. Samples were run on the CEQ8800 sequenzer (Beckman & Coulter).

    Article Title: Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function
    Article Snippet: .. The PCR products were subcloned into T3 vector (Invitrogen™ TOPO™ TA Cloning™ Kit, 450641, Invitrogen, USA) for DNA sequencing (Macrogen Corp., Rockville, MD, USA). .. Retinal Axon Labeling Lipophilic dyes were injected separately into the eyes of zebrafish embryos at 4 dpf using a microinjection system with the following dyes: DiI (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate; Molecular Probes, cat.-no. D-282) and DiD (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine Perchlorate; cat. no. D-307).

    Purification:

    Article Title: Robust activation of microhomology-mediated end joining for precision gene editing applications
    Article Snippet: .. Alternatively, purified amplicons were used for subcloning analysis with either Topo-TA Cloning Kit (Thermo Fisher Scientific 451641. .. Waltham, MA) or StrataClone PCR Cloning Kit (Agilent 240205.

    Article Title: A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish
    Article Snippet: .. The resulting PCRs from the same group of fish were checked on the gel, mixed, purified using QIAquick PCR purification kit (QIAGEN, 28104) and TOPO-cloned into pCR2.1-TOPO (Thermo Fisher Scientific, 450641). .. After transformation, colony PCR was performed on 16 bacterial colonies from a plate for a particular group of F1 mutation carriers.

    Electrophoresis:

    Article Title: Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
    Article Snippet: .. Electrophoresis was done on a 2% TAE gel and the PCR products were cloned by TOPO TA Cloning Kit (Invitrogen, Part no. 45-0641) and clones were prepared for sequencing using the GenomeLab Dye Terminator Cycle Sequencing Kit (Beckman & Coulter, P/N 608120). .. Samples were run on the CEQ8800 sequenzer (Beckman & Coulter).

    Polymerase Chain Reaction:

    Article Title: Analyses of Alternatively Processed Genes in Ciliates Provide Insights into the Origins of Scrambled Genomes and May Provide a Mechanism for Speciation
    Article Snippet: .. Genome walking PCR products were cloned using TA TOPO cloning kits (45-0641; Invitrogen) and screened using the polymerase TaqGold (Applied Biosystems, CA). ..

    Article Title: Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
    Article Snippet: .. Electrophoresis was done on a 2% TAE gel and the PCR products were cloned by TOPO TA Cloning Kit (Invitrogen, Part no. 45-0641) and clones were prepared for sequencing using the GenomeLab Dye Terminator Cycle Sequencing Kit (Beckman & Coulter, P/N 608120). .. Samples were run on the CEQ8800 sequenzer (Beckman & Coulter).

    Article Title: A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish
    Article Snippet: .. The resulting PCRs from the same group of fish were checked on the gel, mixed, purified using QIAquick PCR purification kit (QIAGEN, 28104) and TOPO-cloned into pCR2.1-TOPO (Thermo Fisher Scientific, 450641). .. After transformation, colony PCR was performed on 16 bacterial colonies from a plate for a particular group of F1 mutation carriers.

    Article Title: Electrical coupling and innexin expression in the stomatogastric ganglion of the crab Cancer borealis
    Article Snippet: .. PCR products of appropriate length were cloned into pCR 2.1 TOPO cloning vector (Invitrogen, 45-0641) and verified by sequencing (Genewiz). .. For Inx1 , degenerate primers were designed by hand based on conserved protein sequence alignments from D. melanogaster , Anopheles gambiae , Bombyx mori , and Schistocerca gregaria innexins.

    Article Title: Systematic discovery of novel ciliary genes through functional genomics in the zebrafish
    Article Snippet: .. PCR bands were cloned using the TOPO-TA cloning system (Invitrogen, #450641) and sequenced. cDNA sequences were assembled using a combination of Ensembl gene predictions and EMBL ESTs as references. .. In situ hybridization The lefty2 RNA in situ probe was made by cloning a fragment of the cDNA.

    Article Title: Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function
    Article Snippet: .. The PCR products were subcloned into T3 vector (Invitrogen™ TOPO™ TA Cloning™ Kit, 450641, Invitrogen, USA) for DNA sequencing (Macrogen Corp., Rockville, MD, USA). .. Retinal Axon Labeling Lipophilic dyes were injected separately into the eyes of zebrafish embryos at 4 dpf using a microinjection system with the following dyes: DiI (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate; Molecular Probes, cat.-no. D-282) and DiD (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine Perchlorate; cat. no. D-307).

    DNA Sequencing:

    Article Title: Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function
    Article Snippet: .. The PCR products were subcloned into T3 vector (Invitrogen™ TOPO™ TA Cloning™ Kit, 450641, Invitrogen, USA) for DNA sequencing (Macrogen Corp., Rockville, MD, USA). .. Retinal Axon Labeling Lipophilic dyes were injected separately into the eyes of zebrafish embryos at 4 dpf using a microinjection system with the following dyes: DiI (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate; Molecular Probes, cat.-no. D-282) and DiD (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine Perchlorate; cat. no. D-307).

    Sequencing:

    Article Title: Minimal requirements for reverse polymerization and tRNA repair by tRNAHis guanylyltransferase
    Article Snippet: .. The resulting product was ligated into pCR2.1®-TOPO® TA (ThermoFisher Cat No 451641) for sequencing. ..

    Article Title: Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
    Article Snippet: .. Electrophoresis was done on a 2% TAE gel and the PCR products were cloned by TOPO TA Cloning Kit (Invitrogen, Part no. 45-0641) and clones were prepared for sequencing using the GenomeLab Dye Terminator Cycle Sequencing Kit (Beckman & Coulter, P/N 608120). .. Samples were run on the CEQ8800 sequenzer (Beckman & Coulter).

    Article Title: Electrical coupling and innexin expression in the stomatogastric ganglion of the crab Cancer borealis
    Article Snippet: .. PCR products of appropriate length were cloned into pCR 2.1 TOPO cloning vector (Invitrogen, 45-0641) and verified by sequencing (Genewiz). .. For Inx1 , degenerate primers were designed by hand based on conserved protein sequence alignments from D. melanogaster , Anopheles gambiae , Bombyx mori , and Schistocerca gregaria innexins.

    Fluorescence In Situ Hybridization:

    Article Title: A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish
    Article Snippet: .. The resulting PCRs from the same group of fish were checked on the gel, mixed, purified using QIAquick PCR purification kit (QIAGEN, 28104) and TOPO-cloned into pCR2.1-TOPO (Thermo Fisher Scientific, 450641). .. After transformation, colony PCR was performed on 16 bacterial colonies from a plate for a particular group of F1 mutation carriers.

    Plasmid Preparation:

    Article Title: Electrical coupling and innexin expression in the stomatogastric ganglion of the crab Cancer borealis
    Article Snippet: .. PCR products of appropriate length were cloned into pCR 2.1 TOPO cloning vector (Invitrogen, 45-0641) and verified by sequencing (Genewiz). .. For Inx1 , degenerate primers were designed by hand based on conserved protein sequence alignments from D. melanogaster , Anopheles gambiae , Bombyx mori , and Schistocerca gregaria innexins.

    Article Title: Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function
    Article Snippet: .. The PCR products were subcloned into T3 vector (Invitrogen™ TOPO™ TA Cloning™ Kit, 450641, Invitrogen, USA) for DNA sequencing (Macrogen Corp., Rockville, MD, USA). .. Retinal Axon Labeling Lipophilic dyes were injected separately into the eyes of zebrafish embryos at 4 dpf using a microinjection system with the following dyes: DiI (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate; Molecular Probes, cat.-no. D-282) and DiD (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine Perchlorate; cat. no. D-307).

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  • 96
    Thermo Fisher topo ta cloning kit
    Topo Ta Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/topo ta cloning kit/product/Thermo Fisher
    Average 96 stars, based on 719 article reviews
    Price from $9.99 to $1999.99
    topo ta cloning kit - by Bioz Stars, 2020-08
    96/100 stars
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    Thermo Fisher pcr2 1topo vector
    Pcr2 1topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr2 1topo vector/product/Thermo Fisher
    Average 89 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    pcr2 1topo vector - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr2 1topo ta
    Pcr2 1topo Ta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr2 1topo ta/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcr2 1topo ta - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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