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Meridian Life Science pcr mixture
( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: <t>cDNA</t> as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative <t>PCR</t> control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
Pcr Mixture, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 94/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr mixture/product/Meridian Life Science
Average 94 stars, based on 135 article reviews
Price from $9.99 to $1999.99
pcr mixture - by Bioz Stars, 2020-08
94/100 stars

Images

1) Product Images from "The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering"

Article Title: The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering

Journal: Open Biology

doi: 10.1098/rsob.160212

( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: cDNA as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative PCR control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).
Figure Legend Snippet: ( a ) Transcriptional analysis of the A. pleuropneumoniae NGT locus. Lane 1: cDNA as template; lane 2: RNA as template; lane 3: A. pleuropneumoniae HS143 genomic DNA positive control; lane 4: negative PCR control (no template). ( b ) Transcriptional analysis of the region upstream of ngt . Lane 1: cDNA as template; lane 2: genomic DNA positive control; lane 3: PCR control (RNA as template).

Techniques Used: Positive Control, Polymerase Chain Reaction

2) Product Images from "Optimization of a magnetic capture RT-LAMP assay for fast and real-time detection of potato virus Y and differentiation of N and O serotypes"

Article Title: Optimization of a magnetic capture RT-LAMP assay for fast and real-time detection of potato virus Y and differentiation of N and O serotypes

Journal: Archives of Virology

doi: 10.1007/s00705-017-3635-3

Comparison of sensitivity of the Y4 RT-LAMP, the TaqMan real-time RT-PCR, the Y4 TaqMan real-time RT-PCR, the Y4 SYBR Green real-time PCR (a), and the conventional Y4 RT-PCR (b). (a) For the real-time tests, Cq results are shown as a function of the logarithm of the dilution factor. The data are averages from at least two independent experiments performed in triplicate, and error bars indicate standard deviation. (b) Determination of the sensitivity of PVY detection by RT-PCR in sap from a PVY-infected plant diluted 1 × 10 2 , lane 3; 1 × 10 3 , lane 4; 1 × 10 4 , lane 5; 5 × 10 4 , lane 6; 1 × 10 5 , lane 7; 5 × 10 5 , lane 8; 1 × 10 6 , lane 9; 2 × 10 6 , lane 10. No-template control (PCR reaction containing water instead of cDNA), lane 1; negative control (RT-PCR reaction containing RNA from a virus-free plant), lane 2; Nova 100-bp molecular weight marker (Novazym Poland), lanes M
Figure Legend Snippet: Comparison of sensitivity of the Y4 RT-LAMP, the TaqMan real-time RT-PCR, the Y4 TaqMan real-time RT-PCR, the Y4 SYBR Green real-time PCR (a), and the conventional Y4 RT-PCR (b). (a) For the real-time tests, Cq results are shown as a function of the logarithm of the dilution factor. The data are averages from at least two independent experiments performed in triplicate, and error bars indicate standard deviation. (b) Determination of the sensitivity of PVY detection by RT-PCR in sap from a PVY-infected plant diluted 1 × 10 2 , lane 3; 1 × 10 3 , lane 4; 1 × 10 4 , lane 5; 5 × 10 4 , lane 6; 1 × 10 5 , lane 7; 5 × 10 5 , lane 8; 1 × 10 6 , lane 9; 2 × 10 6 , lane 10. No-template control (PCR reaction containing water instead of cDNA), lane 1; negative control (RT-PCR reaction containing RNA from a virus-free plant), lane 2; Nova 100-bp molecular weight marker (Novazym Poland), lanes M

Techniques Used: Quantitative RT-PCR, SYBR Green Assay, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Standard Deviation, Infection, Polymerase Chain Reaction, Negative Control, Molecular Weight, Marker

3) Product Images from "Decarboxylation of Substituted Cinnamic Acids by Lactic Acid Bacteria Isolated during Malt Whisky Fermentation"

Article Title: Decarboxylation of Substituted Cinnamic Acids by Lactic Acid Bacteria Isolated during Malt Whisky Fermentation

Journal: Applied and Environmental Microbiology

doi:

Induction of pdc mRNA in Lactobacillus strains, as demonstrated by RT-PCR. (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).
Figure Legend Snippet: Induction of pdc mRNA in Lactobacillus strains, as demonstrated by RT-PCR. (A) RT-PCR products from L. pentosus 128 mRNA after exposure of cells to FA at 100 μg/ml for 5 min (lane 1) and 30 min (lane 2) and to PCA at 100 μg/ml for 0 min (lane 4), 5 min (lane 5), and 30 min (lane 6). Ten microliters of RT-PCR product was loaded on the gel. Lane 3, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. crispatus H8 mRNA after exposure of cells to PCA at 100 μg/ml for 0 min (lane 2), 5 min (lane 3), and 30 min (lane 4). Twenty microliters of RT-PCR product was loaded on the gel. Lane 1, PCR (DNA template) control; lane M, 100-bp DNA molecular size marker (Gibco).

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Marker

Induction of pdc mRNA in Lactobacillus strains grown in distiller's wort, as demonstrated by RT-PCR. (A) RT-PCR products from L. fermentum 70 mRNA (lanes 1 to 3) and L. pentosus 128 mRNA (lanes 4 to 6) after exposure for 1 h to wort alone (lanes 1 and 4), wort supplemented with FA at 100 μg/ml (lanes 2 and 5), or wort supplemented with PCA at 100 μg/ml (lanes 3 and 6). Lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. casei 69 mRNA after exposure for 1 h to wort alone (lanes 2 and 4) or wort supplemented with FA at 100 μg/ml (lanes 1 and 3). Lane M, 100-bp DNA molecular size marker (Gibco).
Figure Legend Snippet: Induction of pdc mRNA in Lactobacillus strains grown in distiller's wort, as demonstrated by RT-PCR. (A) RT-PCR products from L. fermentum 70 mRNA (lanes 1 to 3) and L. pentosus 128 mRNA (lanes 4 to 6) after exposure for 1 h to wort alone (lanes 1 and 4), wort supplemented with FA at 100 μg/ml (lanes 2 and 5), or wort supplemented with PCA at 100 μg/ml (lanes 3 and 6). Lane M, 100-bp DNA molecular size marker (Gibco). (B) RT-PCR products from L. casei 69 mRNA after exposure for 1 h to wort alone (lanes 2 and 4) or wort supplemented with FA at 100 μg/ml (lanes 1 and 3). Lane M, 100-bp DNA molecular size marker (Gibco).

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker

Related Articles

Polymerase Chain Reaction:

Article Title: Optimization of a magnetic capture RT-LAMP assay for fast and real-time detection of potato virus Y and differentiation of N and O serotypes
Article Snippet: .. The PCR mixture (10 µl) contained: 1-3 µl of cDNA; 0.2 U of uracil DNA glycosylase (UNG) (Bioline); 0.4 mM dNTPs (including dU instead of dT); 2 mM MgCl2 ; 0.2 mM primers n156, o514, n787, o2172, o2700; 0.1 mM primers n2258, n2650c; 0.6 mM primers n7577, SeroN, Y03-8648; 1 µl of 10-fold-concentrated polymerase buffer, and 0.6 U of GoTaq HotStart Polymerase (Promega). ..

Article Title: Rifampicin Resistance in Tuberculosis Outbreak, London, England
Article Snippet: .. Ten microliters of DNA was added to the PCR mix containing 81.4 μL PCR-quality water, 10 μL potassium chloride buffer (Bioline Ltd., London, UK), 0.4 μL of each primer (100 mmol) (Sigma-Genosys Ltd., Haverhill, UK), 3 μL deoxynucleoside triphosphates (5 mmol), and 1 μL Taq (Bioline). .. The amplification was performed on a Techgene thermal cycler (Techne, Princeton, NJ, USA).

Article Title: Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
Article Snippet: .. Briefly, the PCR mix comprised 5 µl of Bioline 2 × SensiMix SYBR Green (QT650-02), 100 nM each of forward and reverse primer, and 1 µl of sample supernatant to a total of 10 µl per reaction. .. Analysis of the HRM was performed on the Rotorgene software (version 1.7).

Article Title: The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering
Article Snippet: .. For each sample, 2 µl of reverse transcribed cDNA was used as a template in a 25 µl total volume PCR mixture and amplified using MyTaq master mix (Bioline, UK) using the following cycling conditions: 94°C/30 s, followed by 35 cycles of 94°C/10 s, 53°C/10 s, 72°C/10 s and a final single 72°C/30 s cycle using the primers listed in electronic supplementary material, table S2. .. Quantitative real-time PCR validation Total RNA was extracted from bacteria grown in BHI–NAD broth by using Tri Reagent (Sigma, UK) as previously described.

Article Title: Plasmodium malariae in the Colombian Amazon region: you don’t diagnose what you don’t suspect
Article Snippet: .. The PCR mix contained 1× buffer, 3.8 mM MgCl2 , 1.4 mM dNTPs, 0.2 µM primers, 1 U/µl Taq polymerase (BIOLASE DNA Polymerase, Bioline), 2 µl of genomic DNA and molecular grade water (21 µl final volume). ..

Article Title: Decarboxylation of Substituted Cinnamic Acids by Lactic Acid Bacteria Isolated during Malt Whisky Fermentation
Article Snippet: .. The PCR mixture contained 5 μl of 10× Taq DNA polymerase buffer, 1 μl of deoxynucleoside triphosphate mix (12.5 mM), 2 mM MgCl2 , 100 nM each primer, 20 ng of genomic template DNA, and 1 U of Taq DNA polymerase (Bioline, London, United Kingdom) in a final volume of 50 μl. .. DNA amplification was performed for 35 cycles consisting of denaturation for 1 min at 94°C, annealing for 30 s at 50°C, and elongation for 1 min at 72°C with an automated Phoenix DNA Thermocycler (Helena BioSciences, Sunderland, United Kingdom).

Article Title: Vascular Endothelial Growth Factor (VEGF) Bioavailability Regulates Angiogenesis and Intestinal Stem and Progenitor Cell Proliferation during Postnatal Small Intestinal Development
Article Snippet: .. The genotyping PCR mix consisted of 10 μL MyTaq Red Mix (Bioline; Cat# BIO-25043), 0.1 μL 100 μM forward (F) primer, 0.1 μL 100 μM reverse (R) primer (Eurofins MWG Operon, ), 8.8 μL RNase-free water, and 1 μL mouse DNA for a total of 20 μL PCR reaction. .. This was placed in a 0.2 μL PCR tube.

Article Title: Taxonomic circumscription of melanconis-like fungi causing canker disease in China
Article Snippet: .. The PCR mixture for all the regions consisted of 1 μl genomic DNA, 3 mM MgCl2 , 20 μM of each dNTP, 0.2 μM of each primer and 0.25 U BIOTAQ DNA polymerase (Bioline). .. Conditions for PCR of ITS and LSU regions constituted an initial denaturation step of 2 min at 95 °C, followed by 35 cycles of 30 s at 95 °C, 45 s at 51 °C and 1 min at 72 °C and a final extension step of 8 min at 72 °C, while the TEF1-α gene was performed using an initial denaturation step of 2 min at 95 °C, followed by 35 cycles of 30 s at 95 °C, 45 s at 56 °C and 1 min at 72 °C and a final extension step of 8 min at 72 °C.

SYBR Green Assay:

Article Title: Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis
Article Snippet: .. Briefly, the PCR mix comprised 5 µl of Bioline 2 × SensiMix SYBR Green (QT650-02), 100 nM each of forward and reverse primer, and 1 µl of sample supernatant to a total of 10 µl per reaction. .. Analysis of the HRM was performed on the Rotorgene software (version 1.7).

Amplification:

Article Title: The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering
Article Snippet: .. For each sample, 2 µl of reverse transcribed cDNA was used as a template in a 25 µl total volume PCR mixture and amplified using MyTaq master mix (Bioline, UK) using the following cycling conditions: 94°C/30 s, followed by 35 cycles of 94°C/10 s, 53°C/10 s, 72°C/10 s and a final single 72°C/30 s cycle using the primers listed in electronic supplementary material, table S2. .. Quantitative real-time PCR validation Total RNA was extracted from bacteria grown in BHI–NAD broth by using Tri Reagent (Sigma, UK) as previously described.

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    Meridian Life Science pcr master mix
    Pcr Master Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/Meridian Life Science
    Average 92 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-08
    92/100 stars
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