pcr gc buffer i  (TaKaRa)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Buffer PC
    Description:
    Choose lysis wash and binding buffers for genomic DNA isolation using NucleoSpin kits
    Catalog Number:
    740937
    Price:
    None
    Size:
    125 mL
    Category:
    Genomic DNA isolation Buffers enzymes Accessories and components Nucleic acid purification
    Buy from Supplier


    Structured Review

    TaKaRa pcr gc buffer i
    Choose lysis wash and binding buffers for genomic DNA isolation using NucleoSpin kits
    https://www.bioz.com/result/pcr gc buffer i/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr gc buffer i - by Bioz Stars, 2020-09
    94/100 stars

    Related Products / Commonly Used Together

    dna taq polymerase
    vntr locus
    dntp

    Images

    Related Articles

    DNA Extraction:

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Amplification:

    Article Title: Effect of Nano-SiO2 on Expression and Aberrant Methylation of Imprinted Genes in Lung and Testis
    Article Snippet: .. Each DNA sample was amplified by PCR as follows: 2.5 μl 10 × PCR buffer PCR reaction mix with 500 ng the bisulfite-treated DNA, 0.5 μl each of forward and reverse primers, 0.5 μl dNTP Mix, 0.5 μl rTaq (500 U, dNTP, Mg2+ ) (Takara Bio, Tokyo, Japan), addition of ddH2 O up to a volume of 25 μl. .. A549 cells were purchased from ATCC (Manassas, VA, USA) and were cultured in 1640 supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO2 in a humidified incubator.

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Transferring:

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Polymerase Chain Reaction:

    Article Title: Expression and Molecular Evolution of Two DREB1 Genes in Black Poplar (Populus nigra)
    Article Snippet: .. To amplify genomic DNA, reactions were carried out in a volume of 50 µl containing 50 ng of template DNA, 0.2 mM of each dNTP, 0.5 µM of each primer, 1× PCR buffer plus MgCl2 , and 0.5 U LA-Taq polymerase (TaKaRa, Dalian, China). .. All PCR reactions were run on a PE 9700 Thermal Cycler (Applied Biosystems, Foster City, CA, USA).

    Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
    Article Snippet: .. 10 μM forward primer (F1 or F2, if desired) 10 μM reverse primer (R1 or R2, if desired) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57 [ ] .. Use reagents in section 2.4, but use the heterozygous yfg1::dpl200/YFG1 strain as the transformation recipient instead of YFG1/YFG1 strain BWP17.

    Article Title: Identification of novel SNPs of ABCD1, ABCD2, ABCD3, and ABCD4 genes in patients with X-linked adrenoleukodystrophy (ALD) based on comprehensive resequencing and association studies with ALD phenotypes
    Article Snippet: .. The PCR conditions were as follows: 94°C for 1 min, followed by five cycles consisting of 94°C for 30 s, 62°C for 30 s, and 68°C for 2 min; five cycles consisting of 94°C for 30 s, 60°C for 30 s, and 68°C for 2 min; and 25 cycles consisting of 94°C for 30 s, 58°C for 30 s, and 68°C for 2 min, followed by a final extension at 68°C for 7 min, using the LA Taq with GC Buffer PCR system (Takara Bio, Otsu, Shiga, Japan). ..

    Article Title: Effect of Nano-SiO2 on Expression and Aberrant Methylation of Imprinted Genes in Lung and Testis
    Article Snippet: .. Each DNA sample was amplified by PCR as follows: 2.5 μl 10 × PCR buffer PCR reaction mix with 500 ng the bisulfite-treated DNA, 0.5 μl each of forward and reverse primers, 0.5 μl dNTP Mix, 0.5 μl rTaq (500 U, dNTP, Mg2+ ) (Takara Bio, Tokyo, Japan), addition of ddH2 O up to a volume of 25 μl. .. A549 cells were purchased from ATCC (Manassas, VA, USA) and were cultured in 1640 supplemented with 10% fetal bovine serum (FBS) at 37 °C and 5% CO2 in a humidified incubator.

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
    Article Snippet: .. 10 μM forward primer (F1) 10 μM reverse primer (R1) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57, Pubmed accession number: [ ] .. C. albicans strain BWP17 [ ] YPD+Uri (80 μg/μL) plates: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 2% bacto-agar, 80 μg/μL of uridine YPD+Uri (80 μg/μL) liquid medium: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 80 μg/μL of uridine Ura-blister cassette PCR product LATE (0.1M LiOAc in 1X TE buffer): 0.372g/L 1mM EDTA disodium salt, 1.21g/L Tris, 10.2g/L Lithium acetate, pH 7.5 Calf thymus DNA (~10 mg/mL) (Sigma D8661-1ML) PLATE (8 mL 50% w/v PEG3350 (Sigma) + 1 mL 10X TE + 1 mL 1 M LiOAc): For 50% w/v PEG3350 dissolve 50g PEG3350 in 100 mL (final volume) of distilled water and filter sterilize after mixing; For 10x TE 3.72g/L 1mM EDTA disodium salt, 12.1g 10mM TRIS, pH 7.5; For 1M LiOAc 102g/L Lithium acetate pH 7.5.

    Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
    Article Snippet: .. 10 μM Comp forward primer (CF) 10 μM comp reverse primer (CR) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Genomic DNA: Reference strain ( see ) .. S. cerevisiae BY4741Δ trp strain [ ] YPD plates (see item 2, section 2.4) YPD liquid medium (see item 3, section 2.4) pDDB78 plasmid, Pubmed accession number pending [ ] PCR product: wild type gene amplified using complementation primers Restriction Enzymes NotI and EcoRI (New England Biolabs) 10X NE Buffer 3 (New England Biolabs) 100X BSA (New England Biolabs) Calf thymus DNA (~10 mg/mL) (Sigma) PLATE (8 mL 50% PEG3350 (Sigma) + 1 mL 10X TE + 1 mL 1 M LiOAc) CSM-TRP plates: 2% glucose, 0.67% yeast nitrogen base (without amino acids), 0.074% of CSM-TRP dropout medium (MP Biomedicals, LLC), 2% bacto-agar CSM-TRP liquid medium: 2% glucose, 0.67% yeast nitrogen base (without amino acids), 0.074% of CSM-URA dropout medium (MP Biomedicals, LLC) Plasmid DNA Miniprep kit (Fermentas GeneJET™ Plasmid Miniprep Kit) Acid washed glass beads (size:425–600μm)

    Incubation:

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Sequencing:

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Lysis:

    Article Title: Phylogenetic diversity of culturable fungi in the Heshang Cave, central China
    Article Snippet: .. Genomic DNA Extraction, rRNA-ITS Gene Amplification, and Sequencing Fungal mycelia on PDA plates after 5 days of incubation at 25°C were scraped by sterile pipette tips and cells were broken with 50 μl lysis buffer [Lysis Buffer for Microorganism to Direct polymerase chain reaction (PCR), TaKaRa] in 1.5 ml micro-centrifuge tubes. .. Genomic DNA was extracted according to the instruction of the direct PCR (TaKaRa) method with modifications of the first step as followings: (1) incubation at 80°C coupled with oscillation for 10 min, followed by, (2) -80°C for 15 min, (3) thermal denaturation at 80°C with oscillation for 15 min, and (4) centrifugation at 5000 rpm for 5 min at 22°C.

    Plasmid Preparation:

    Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
    Article Snippet: .. 10 μM forward primer (F1 or F2, if desired) 10 μM reverse primer (R1 or R2, if desired) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57 [ ] .. Use reagents in section 2.4, but use the heterozygous yfg1::dpl200/YFG1 strain as the transformation recipient instead of YFG1/YFG1 strain BWP17.

    Article Title: Mini-blaster-mediated targeted gene disruption and marker complementation in C. albicans
    Article Snippet: .. 10 μM forward primer (F1) 10 μM reverse primer (R1) 10X Ex Taq Buffer PCR buffer (contains 20 mM Mg2+ ) dNTPs (2.5mM each) TaKaRa Ex Taq ™ DNA polymerase (Takara Bio Inc) Template Plasmid: pDDB57, Pubmed accession number: [ ] .. C. albicans strain BWP17 [ ] YPD+Uri (80 μg/μL) plates: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 2% bacto-agar, 80 μg/μL of uridine YPD+Uri (80 μg/μL) liquid medium: 2% glucose, 2% bacto-peptone, 1% bacto-yeast extract, 80 μg/μL of uridine Ura-blister cassette PCR product LATE (0.1M LiOAc in 1X TE buffer): 0.372g/L 1mM EDTA disodium salt, 1.21g/L Tris, 10.2g/L Lithium acetate, pH 7.5 Calf thymus DNA (~10 mg/mL) (Sigma D8661-1ML) PLATE (8 mL 50% w/v PEG3350 (Sigma) + 1 mL 10X TE + 1 mL 1 M LiOAc): For 50% w/v PEG3350 dissolve 50g PEG3350 in 100 mL (final volume) of distilled water and filter sterilize after mixing; For 10x TE 3.72g/L 1mM EDTA disodium salt, 12.1g 10mM TRIS, pH 7.5; For 1M LiOAc 102g/L Lithium acetate pH 7.5.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    TaKaRa gc buffer i
    Gc Buffer I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gc buffer i/product/TaKaRa
    Average 91 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    gc buffer i - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    TaKaRa 1x gc buffer i
    1x Gc Buffer I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x gc buffer i/product/TaKaRa
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    1x gc buffer i - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    95
    TaKaRa pcr buffer
    Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1840 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/TaKaRa
    Average 95 stars, based on 1840 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results