pcr cloningplus kit  (Qiagen)


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    Name:
    QIAGEN PCR Cloningplus Kit
    Description:
    For direct cloning of PCR products Kit contents Qiagen PCR Cloning plus Kit 10 rxns EZ Competent Cells U Overhang UA Hybridization Reaction Type pDrive Cloning Vector For Direct Cloning of PCR Products Includes 2x Ligation Master Mix 50L pDrive Cloning Vector 0 5g Distilled water 1 7mL Qiagen EZ Competent Cells 10 Tubes 50L Each SOC medium 2 x 1 9mL Benefits Just 40 minutes from PCR product to plated cells Ready to use Ligation Master Mix High specificity UA hybridization for efficient cloning Competent cells supplied with the kit Immediate plating of transformed competent ce
    Catalog Number:
    231222
    Price:
    285
    Category:
    QIAGEN PCR Cloningplus Kit
    Buy from Supplier


    Structured Review

    Qiagen pcr cloningplus kit
    QIAGEN PCR Cloningplus Kit
    For direct cloning of PCR products Kit contents Qiagen PCR Cloning plus Kit 10 rxns EZ Competent Cells U Overhang UA Hybridization Reaction Type pDrive Cloning Vector For Direct Cloning of PCR Products Includes 2x Ligation Master Mix 50L pDrive Cloning Vector 0 5g Distilled water 1 7mL Qiagen EZ Competent Cells 10 Tubes 50L Each SOC medium 2 x 1 9mL Benefits Just 40 minutes from PCR product to plated cells Ready to use Ligation Master Mix High specificity UA hybridization for efficient cloning Competent cells supplied with the kit Immediate plating of transformed competent ce
    https://www.bioz.com/result/pcr cloningplus kit/product/Qiagen
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    pcr cloningplus kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Multiple splice defects in ABCA1 cause low HDL-C in a family with Hypoalphalipoproteinemia and premature coronary disease
    Article Snippet: .. The PCR products were then cloned into a pDrive Cloning Vector, Qiagen PCR Cloningplus Kit (Qiagen, Inc, Valencia, CA.), plated, and grown overnight. .. Positive colonies were PCR-screened using the primers listed above.

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: .. Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations. .. Plasmid DNA was extracted using the Nucleospin plasmid kit (Macherey-Nagel, Düren, Germany).

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: .. The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. The cloned fragments were identified using blue/white screening on Lennox-L-Agar plates containing ampicillin, X-gal, and isopropyl-β-d -l-thiogalactopyranoside (IPTG).

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: .. Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit. ..

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions. .. The positive clones underwent automatic sequencing for both strands at the McLab company (San Francisco, CA).

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions. .. A total of 224 insert-positive clones were sequenced.

    Article Title: Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions
    Article Snippet: .. The primer set of 27f (5′-AGAGTTTGATCMTGGCTC AG-3′) and 1492r (5′-TACGGYTACCTTGTTACGACTT-3′), and the QIAGEN PCR CloningPLUS Kit (Qiagen) were used for amplification and cloning, respectively. .. The cloned 16S rRNA genes were subjected to T-RFLP analysis to identify clones with the same fragment length of the presumptive benzene-assimilating bacteria.

    Article Title: Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay ▿
    Article Snippet: .. PCR amplicons that resulted from C. pneumoniae cell cultures were cloned using the QIAquick PCR purification kit and the Qiagen PCR CloningPlus kit (Qiagen, Hilden, Germany), which contains competent cells. ..

    Amplification:

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: For the construction of clone libraries, nearly full-length bacterial 16S rRNA gene sequences of a subset of the bacterial community were amplified by PCR using primers 26F and SAP-1490R (modified from the primers in reference ). .. Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations.

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: The amplified fragments, ranging from 400 to 800 bp, were then separated on a 1.5% LO3 agarose gel (TaKaRa Bio Inc.) and purified using Gene Clean II Kit (Qbiogene, Solon, Ohio, USA). .. The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions.

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions. .. A total of 224 insert-positive clones were sequenced.

    Article Title: Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions
    Article Snippet: .. The primer set of 27f (5′-AGAGTTTGATCMTGGCTC AG-3′) and 1492r (5′-TACGGYTACCTTGTTACGACTT-3′), and the QIAGEN PCR CloningPLUS Kit (Qiagen) were used for amplification and cloning, respectively. .. The cloned 16S rRNA genes were subjected to T-RFLP analysis to identify clones with the same fragment length of the presumptive benzene-assimilating bacteria.

    DNA Ligation:

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: The restriction fragments were then ligated with a specific blunt adapter (consisting of the 48-mer: 5′-GTAATACGACTCACTATAGGGCACGCGTGGTCGACGGCCCGGGCTGGT-3′ and an 8-mer with the 3′-end capped with an amino residue: 5′-ACCAGCCC-NH2 -3′) using a DNA ligation kit (TaKaRa Bio Inc., Ohtsu, Shiga, Japan). .. The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions.

    Construct:

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: .. Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit. ..

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: An adapter-ligated DNA library was constructed as described previously ( ). .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: 1 µg of previously extracted RNA underwent real-time polymerase chain reaction (RT-PCR) with the Qiagen OneStep RT-PCR kit. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    Incubation:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: The RT-PCR conditions were an initial preincubation at 50°C for 30 minutes (RT condition), followed by an incubation at 94°C for 15 minutes (initial PCR activation); 37 cycles at 97°C for 1 minute (denaturation), at 60°C for 1 minute (annealing), and at 72°C for 1 minute (extension); and a final extension at 72°C for 10 minutes. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    Infection:

    Article Title: Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay ▿
    Article Snippet: We then used mupirocin treatment of C. pneumoniae -infected cells in an established and effective procedure for elimination of mycoplasma infection ( ). .. PCR amplicons that resulted from C. pneumoniae cell cultures were cloned using the QIAquick PCR purification kit and the Qiagen PCR CloningPlus kit (Qiagen, Hilden, Germany), which contains competent cells.

    Expressing:

    Article Title: Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature
    Article Snippet: Paragraph title: Identification of M. sexta Trp genes and analysis of TrpA1 expression in chemosensory tissues (Experiment 2) ... We confirmed the identity of the 204-bp-amplified product by subcloning it into the pDrive vector (Qiagen cat #231224) and sequencing it (Genewiz).

    Modification:

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: For the construction of clone libraries, nearly full-length bacterial 16S rRNA gene sequences of a subset of the bacterial community were amplified by PCR using primers 26F and SAP-1490R (modified from the primers in reference ). .. Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations.

    Transformation Assay:

    Article Title: Over-Expression of the Pikh Gene with a CaMV 35S Promoter Leads to Improved Blast Disease (Magnaporthe oryzae) Tolerance in Rice
    Article Snippet: Constructing recombinant plasmids The purified PCR product (1206 bp) was directly inserted into the pDriveU/A cloning vector (QIAGEN, Hilden, North Rhine Westphalia, Germany) using a PCR Cloningplus Kit (QIAGEN, Hilden, North Rhine Westphalia, Germany). .. The ligated pDrive vectors were then transformed into E. coli EZ cells and cultured overnight (16 h, 37°C) in LB agar medium containing X-gal, IPTG, and ampicillin.

    Article Title: Screening and Expression of a Silicon Transporter Gene (Lsi1) in Wild-Type Indica Rice Cultivars
    Article Snippet: .. The transformation process was performed according to the instructions provided by the manufacturer of the PCR Cloningplus Kit (Qiagen, Germany). ..

    Sequencing:

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: Paragraph title: PCR and sequencing. ... Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations.

    Article Title: Hypolithic Microbial Community of Quartz Pavement in the High-Altitude Tundra of Central Tibet
    Article Snippet: Paragraph title: Clone Library Construction and Sequencing ... Each pooled sample was gel-purified and used as template for construction of the three clone libraries (Qiagen PCR Cloningplus kit, CA, US).

    Article Title: Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature
    Article Snippet: .. We confirmed the identity of the 204-bp-amplified product by subcloning it into the pDrive vector (Qiagen cat #231224) and sequencing it (Genewiz). ..

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: Paragraph title: Cloning and sequencing. ... Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit.

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions. .. The positive clones underwent automatic sequencing for both strands at the McLab company (San Francisco, CA).

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions. .. We identified 33 distinct sequences containing an (AC)6 (AG)n or (AC)6 (TC)n compound SSR sequence at one end using ClustalX version 2.1 ( ).

    Article Title: Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions
    Article Snippet: Paragraph title: Sequencing of the heavy fractions ... The primer set of 27f (5′-AGAGTTTGATCMTGGCTC AG-3′) and 1492r (5′-TACGGYTACCTTGTTACGACTT-3′), and the QIAGEN PCR CloningPLUS Kit (Qiagen) were used for amplification and cloning, respectively.

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: Comparison of bacterial DNA amplified from PCRs with extracted template DNA and reactions with small pieces of 0.2-μm-pore-size polycarbonate filters from the same sample by DGGE (according to the protocol described in reference ) did not yield any qualitative differences in the microbial fingerprints obtained. .. Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations.

    SYBR Green Assay:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: Each reaction product (10 µL) underwent electrophoresis in agarose gels at 1% that contained SYBR Green I Nucleic Acid Gel Stain (Invitrogen, Carlsbad, CA) and they were visualized on a UV light transilluminator. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    Cell Culture:

    Article Title: Over-Expression of the Pikh Gene with a CaMV 35S Promoter Leads to Improved Blast Disease (Magnaporthe oryzae) Tolerance in Rice
    Article Snippet: Constructing recombinant plasmids The purified PCR product (1206 bp) was directly inserted into the pDriveU/A cloning vector (QIAGEN, Hilden, North Rhine Westphalia, Germany) using a PCR Cloningplus Kit (QIAGEN, Hilden, North Rhine Westphalia, Germany). .. The ligated pDrive vectors were then transformed into E. coli EZ cells and cultured overnight (16 h, 37°C) in LB agar medium containing X-gal, IPTG, and ampicillin.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature
    Article Snippet: In brief, we conducted RT-PCR in 50-µL reactions using Invitrogen Taq polymerase (cat #10342-020) under the following conditions: 2.5U Taq , 20mM Tris pH 8.4, 40mM KCl, 1.5mM MgCl2 , 10mM each deoxyribonucleotide triphosphate, 40 pmol each primer, and 0.5 µL cDNA. .. We confirmed the identity of the 204-bp-amplified product by subcloning it into the pDrive vector (Qiagen cat #231224) and sequencing it (Genewiz).

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: The RT-PCR conditions were an initial preincubation at 50°C for 30 minutes (RT condition), followed by an incubation at 94°C for 15 minutes (initial PCR activation); 37 cycles at 97°C for 1 minute (denaturation), at 60°C for 1 minute (annealing), and at 72°C for 1 minute (extension); and a final extension at 72°C for 10 minutes. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    Recombinant:

    Article Title: Over-Expression of the Pikh Gene with a CaMV 35S Promoter Leads to Improved Blast Disease (Magnaporthe oryzae) Tolerance in Rice
    Article Snippet: .. Constructing recombinant plasmids The purified PCR product (1206 bp) was directly inserted into the pDriveU/A cloning vector (QIAGEN, Hilden, North Rhine Westphalia, Germany) using a PCR Cloningplus Kit (QIAGEN, Hilden, North Rhine Westphalia, Germany). .. The ligated pDrive vectors were then transformed into E. coli EZ cells and cultured overnight (16 h, 37°C) in LB agar medium containing X-gal, IPTG, and ampicillin.

    DNA Extraction:

    Article Title: Hypolithic Microbial Community of Quartz Pavement in the High-Altitude Tundra of Central Tibet
    Article Snippet: Each pooled sample was gel-purified and used as template for construction of the three clone libraries (Qiagen PCR Cloningplus kit, CA, US). .. Plasmids were extracted from positive transformants (Mini-M™ Plasmid DNA extraction system, Viogene, Taiwan) and screened by restriction fragment length polymorphism (RFLP) using the restriction endonucleases MspI and CfoI.

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: Fresh leaves were sampled from one of the seedlings for DNA extraction to construct an SSR library. .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions.

    Molecular Cloning:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions. .. The positive clones underwent automatic sequencing for both strands at the McLab company (San Francisco, CA).

    Isolation:

    Article Title: Multiple splice defects in ABCA1 cause low HDL-C in a family with Hypoalphalipoproteinemia and premature coronary disease
    Article Snippet: The PCR products were then cloned into a pDrive Cloning Vector, Qiagen PCR Cloningplus Kit (Qiagen, Inc, Valencia, CA.), plated, and grown overnight. .. Plasmid DNA was isolated using the Quantum Prep Plasmid Miniprep Kit (Bio-Rad Laboratories, Hercules, CA).

    Subcloning:

    Article Title: Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature
    Article Snippet: .. We confirmed the identity of the 204-bp-amplified product by subcloning it into the pDrive vector (Qiagen cat #231224) and sequencing it (Genewiz). ..

    Labeling:

    Article Title: Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions
    Article Snippet: Sequencing of the heavy fractions To determine the taxonomic classification of the T-RFs that were increased in the heavy fraction in the labeled treatment, almost full-length bacterial 16S rRNA genes in one of the heavy fractions (d = 1.729 g cm−3 ) of the 13 C-treatment on day 64 were amplified and cloned. .. The primer set of 27f (5′-AGAGTTTGATCMTGGCTC AG-3′) and 1492r (5′-TACGGYTACCTTGTTACGACTT-3′), and the QIAGEN PCR CloningPLUS Kit (Qiagen) were used for amplification and cloning, respectively.

    Purification:

    Article Title: Multiple splice defects in ABCA1 cause low HDL-C in a family with Hypoalphalipoproteinemia and premature coronary disease
    Article Snippet: Cleaning, cloning and screening of ABCA1 cDNA fragments Following PCR, ABCA1 cDNA fragments containing exons 6–8, and 30–33 were cleaned for cloning with the Qiaquick PCR Purification Kit (Qiagen, Inc, Valencia, CA.). .. The PCR products were then cloned into a pDrive Cloning Vector, Qiagen PCR Cloningplus Kit (Qiagen, Inc, Valencia, CA.), plated, and grown overnight.

    Article Title: Over-Expression of the Pikh Gene with a CaMV 35S Promoter Leads to Improved Blast Disease (Magnaporthe oryzae) Tolerance in Rice
    Article Snippet: .. Constructing recombinant plasmids The purified PCR product (1206 bp) was directly inserted into the pDriveU/A cloning vector (QIAGEN, Hilden, North Rhine Westphalia, Germany) using a PCR Cloningplus Kit (QIAGEN, Hilden, North Rhine Westphalia, Germany). .. The ligated pDrive vectors were then transformed into E. coli EZ cells and cultured overnight (16 h, 37°C) in LB agar medium containing X-gal, IPTG, and ampicillin.

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: .. The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. The cloned fragments were identified using blue/white screening on Lennox-L-Agar plates containing ampicillin, X-gal, and isopropyl-β-d -l-thiogalactopyranoside (IPTG).

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: .. Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit. ..

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions. .. The positive clones underwent automatic sequencing for both strands at the McLab company (San Francisco, CA).

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions. .. A total of 224 insert-positive clones were sequenced.

    Article Title: Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay ▿
    Article Snippet: .. PCR amplicons that resulted from C. pneumoniae cell cultures were cloned using the QIAquick PCR purification kit and the Qiagen PCR CloningPlus kit (Qiagen, Hilden, Germany), which contains competent cells. ..

    Polymerase Chain Reaction:

    Article Title: Multiple splice defects in ABCA1 cause low HDL-C in a family with Hypoalphalipoproteinemia and premature coronary disease
    Article Snippet: .. The PCR products were then cloned into a pDrive Cloning Vector, Qiagen PCR Cloningplus Kit (Qiagen, Inc, Valencia, CA.), plated, and grown overnight. .. Positive colonies were PCR-screened using the primers listed above.

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: .. Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations. .. Plasmid DNA was extracted using the Nucleospin plasmid kit (Macherey-Nagel, Düren, Germany).

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: .. The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. The cloned fragments were identified using blue/white screening on Lennox-L-Agar plates containing ampicillin, X-gal, and isopropyl-β-d -l-thiogalactopyranoside (IPTG).

    Article Title: Hypolithic Microbial Community of Quartz Pavement in the High-Altitude Tundra of Central Tibet
    Article Snippet: .. Each pooled sample was gel-purified and used as template for construction of the three clone libraries (Qiagen PCR Cloningplus kit, CA, US). .. Plasmids were extracted from positive transformants (Mini-M™ Plasmid DNA extraction system, Viogene, Taiwan) and screened by restriction fragment length polymorphism (RFLP) using the restriction endonucleases MspI and CfoI.

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: .. Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit. ..

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions. .. The positive clones underwent automatic sequencing for both strands at the McLab company (San Francisco, CA).

    Article Title: Screening and Expression of a Silicon Transporter Gene (Lsi1) in Wild-Type Indica Rice Cultivars
    Article Snippet: .. The transformation process was performed according to the instructions provided by the manufacturer of the PCR Cloningplus Kit (Qiagen, Germany). ..

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions. .. A total of 224 insert-positive clones were sequenced.

    Article Title: Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions
    Article Snippet: .. The primer set of 27f (5′-AGAGTTTGATCMTGGCTC AG-3′) and 1492r (5′-TACGGYTACCTTGTTACGACTT-3′), and the QIAGEN PCR CloningPLUS Kit (Qiagen) were used for amplification and cloning, respectively. .. The cloned 16S rRNA genes were subjected to T-RFLP analysis to identify clones with the same fragment length of the presumptive benzene-assimilating bacteria.

    Article Title: Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay ▿
    Article Snippet: .. PCR amplicons that resulted from C. pneumoniae cell cultures were cloned using the QIAquick PCR purification kit and the Qiagen PCR CloningPlus kit (Qiagen, Hilden, Germany), which contains competent cells. ..

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Hypolithic Microbial Community of Quartz Pavement in the High-Altitude Tundra of Central Tibet
    Article Snippet: Clone Library Construction and Sequencing Since no significant difference between individual rRNA gene-defined rock communities could be determined using t-RFLP, equimolar mixtures of pooled PCR amplicons from each quadrat were used in the construction of three separate clone libraries for archaea, bacteria, and eukarya. .. Each pooled sample was gel-purified and used as template for construction of the three clone libraries (Qiagen PCR Cloningplus kit, CA, US).

    Article Title: Time-Resolved DNA Stable Isotope Probing Links Desulfobacterales- and Coriobacteriaceae-Related Bacteria to Anaerobic Degradation of Benzene under Methanogenic Conditions
    Article Snippet: The primer set of 27f (5′-AGAGTTTGATCMTGGCTC AG-3′) and 1492r (5′-TACGGYTACCTTGTTACGACTT-3′), and the QIAGEN PCR CloningPLUS Kit (Qiagen) were used for amplification and cloning, respectively. .. The cloned 16S rRNA genes were subjected to T-RFLP analysis to identify clones with the same fragment length of the presumptive benzene-assimilating bacteria.

    Staining:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: Each reaction product (10 µL) underwent electrophoresis in agarose gels at 1% that contained SYBR Green I Nucleic Acid Gel Stain (Invitrogen, Carlsbad, CA) and they were visualized on a UV light transilluminator. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Multiple splice defects in ABCA1 cause low HDL-C in a family with Hypoalphalipoproteinemia and premature coronary disease
    Article Snippet: .. The PCR products were then cloned into a pDrive Cloning Vector, Qiagen PCR Cloningplus Kit (Qiagen, Inc, Valencia, CA.), plated, and grown overnight. .. Positive colonies were PCR-screened using the primers listed above.

    Article Title: Diversity and Phylogenetic Affiliations of Morphologically Conspicuous Large Filamentous Bacteria Occurring in the Pelagic Zones of a Broad Spectrum of Freshwater Habitats
    Article Snippet: Cloning was performed with the QIAGEN PCR Cloningplus kit following the manufacturer's recommendations. .. Plasmid DNA was extracted using the Nucleospin plasmid kit (Macherey-Nagel, Düren, Germany).

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions. .. Two hundred forty insert-positive clones were amplified using the M13 forward and reverse primers from the plasmid DNA.

    Article Title: Hypolithic Microbial Community of Quartz Pavement in the High-Altitude Tundra of Central Tibet
    Article Snippet: Each pooled sample was gel-purified and used as template for construction of the three clone libraries (Qiagen PCR Cloningplus kit, CA, US). .. Plasmids were extracted from positive transformants (Mini-M™ Plasmid DNA extraction system, Viogene, Taiwan) and screened by restriction fragment length polymorphism (RFLP) using the restriction endonucleases MspI and CfoI.

    Article Title: Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature
    Article Snippet: .. We confirmed the identity of the 204-bp-amplified product by subcloning it into the pDrive vector (Qiagen cat #231224) and sequencing it (Genewiz). ..

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit. .. Transformant plasmid DNA was purified using the standard “lysis-by-alkali” protocol ( ) and sequenced on a model CEQ 8000 genetic analysis system (Beckman Coulter, Fullerton, CA) with an M13 reverse universal primer.

    Article Title: Screening and Expression of a Silicon Transporter Gene (Lsi1) in Wild-Type Indica Rice Cultivars
    Article Snippet: The ccdB gene, which is found in the structure of the pDONOR™/Zeo vector, functions as a negative selectable marker. .. The transformation process was performed according to the instructions provided by the manufacturer of the PCR Cloningplus Kit (Qiagen, Germany).

    Article Title: Sequence Homologies between Mycoplasma and Chlamydia spp. Lead to False-Positive Results in Chlamydial Cell Cultures Tested for Mycoplasma Contamination with a Commercial PCR Assay ▿
    Article Snippet: PCR amplicons that resulted from C. pneumoniae cell cultures were cloned using the QIAquick PCR purification kit and the Qiagen PCR CloningPlus kit (Qiagen, Hilden, Germany), which contains competent cells. .. Plasmid DNA was purified with a plasmid purification kit (Macherey-Nagel, Düren, Germany).

    Software:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions. .. The sequences obtained were compared with those published in the NCBI using the Nucleotide Blast software.

    Electrophoresis:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: Each reaction product (10 µL) underwent electrophoresis in agarose gels at 1% that contained SYBR Green I Nucleic Acid Gel Stain (Invitrogen, Carlsbad, CA) and they were visualized on a UV light transilluminator. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    RNA Extraction:

    Article Title: Gustatory Receptor Neurons in Manduca sexta Contain a TrpA1-Dependent Signaling Pathway that Integrates Taste and Temperature
    Article Snippet: We performed tissue dissections, RNA extraction, and cDNA synthesis as described previously ( ) from larvae 2 days after molting to the fifth instar. .. We confirmed the identity of the 204-bp-amplified product by subcloning it into the pDrive vector (Qiagen cat #231224) and sequencing it (Genewiz).

    Selection:

    Article Title: Screening and Expression of a Silicon Transporter Gene (Lsi1) in Wild-Type Indica Rice Cultivars
    Article Snippet: Zeocin was used for bacterial selection in low-salt LB agar. .. The transformation process was performed according to the instructions provided by the manufacturer of the PCR Cloningplus Kit (Qiagen, Germany).

    Agarose Gel Electrophoresis:

    Article Title: Development of microsatellite markers for Vitex rotundifolia (Verbenaceae), an endangered coastal plant in Lake Biwa, Japan 1
    Article Snippet: The amplified fragments, ranging from 400 to 800 bp, were then separated on a 1.5% LO3 agarose gel (TaKaRa Bio Inc.) and purified using Gene Clean II Kit (Qbiogene, Solon, Ohio, USA). .. The purified DNA fragments were cloned using the QIAGEN PCR Cloningplus Kit (QIAGEN, Hilden, Germany) following the manufacturer’s instructions.

    Activation Assay:

    Article Title: Dengue Virus Coinfection in Human Immunodeficiency Virus-1-Infected Patients on the West Coast of Mexico
    Article Snippet: The RT-PCR conditions were an initial preincubation at 50°C for 30 minutes (RT condition), followed by an incubation at 94°C for 15 minutes (initial PCR activation); 37 cycles at 97°C for 1 minute (denaturation), at 60°C for 1 minute (annealing), and at 72°C for 1 minute (extension); and a final extension at 72°C for 10 minutes. .. The DNA products were purified directly from the gel using the QIAquick Spin Kit (Qiagen) and underwent molecular cloning with the Qiagen PCR CloningPlus kit, following the manufacturer’s instructions.

    Lysis:

    Article Title: No Evidence of an Impact on the Rhizosphere Diazotroph Community by the Expression of Bacillus thuringiensis Cry1Ab Toxin by Bt White Spruce ▿ Cry1Ab Toxin by Bt White Spruce ▿ †
    Article Snippet: Five clone libraries were constructed (control, GUS, Bt/GUS, Wild-VC, and Wild-SF) by cloning purified products using a QIAGEN PCR CloningPlus kit. .. Transformant plasmid DNA was purified using the standard “lysis-by-alkali” protocol ( ) and sequenced on a model CEQ 8000 genetic analysis system (Beckman Coulter, Fullerton, CA) with an M13 reverse universal primer.

    Marker:

    Article Title: Screening and Expression of a Silicon Transporter Gene (Lsi1) in Wild-Type Indica Rice Cultivars
    Article Snippet: The ccdB gene, which is found in the structure of the pDONOR™/Zeo vector, functions as a negative selectable marker. .. The transformation process was performed according to the instructions provided by the manufacturer of the PCR Cloningplus Kit (Qiagen, Germany).

    Gel Extraction:

    Article Title: Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo 1
    Article Snippet: .. The amplified fragments of 400–1000 bp were purified using a QIAquick Gel Extraction Kit (QIAGEN, Valencia, California, USA) and cloned using a QIAGEN PCR Cloningplus Kit (QIAGEN) in accordance with the manufacturer’s instructions. .. A total of 224 insert-positive clones were sequenced.

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    Qiagen pcr cloningplus kit
    Pcr Cloningplus Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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