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Promega pcr clean up system purification kit
Pcr Clean Up System Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean up system purification kit/product/Promega
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pcr clean up system purification kit - by Bioz Stars, 2020-04
93/100 stars

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DNA Extraction:

Article Title: First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax
Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequence analysis ... PCR products were purified using a PCR Clean-up System purification kit (Promega, Madison, WI, USA) and sequenced using BigDye terminator cycle sequence kits (Applied Biosystems) with a ABI Prism 310 genetic analyzer (Applied Biosystems).

Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
Article Snippet: Paragraph title: 2.3. DNA Extraction and PCR Amplification ... The PCR products were purified by using a Wizard SV gel and PCR Clean-Up System purification kit (Promega Italia, Milan, Italy) and quantified by electrophoresis in agarose gel with the quantitative LowRanger 100 bp DNA Ladder (Norgen Biotek Corp., Thorold, Ontario, Canada).

Amplification:

Article Title: High taxonomic level fingerprint of the human intestinal microbiota by Ligase Detection Reaction - Universal Array approach
Article Snippet: PCR products were purified by using a Wizard SV gel and PCR clean-up System purification kit (Promega Italia, Milan, Italy), according to the manufacturer's instructions, eluted in 20 μl of sterile water, and quantified with the DNA 7500 LabChip Assay kit and BioAnalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). .. 16S rRNA was amplified using universal forward primer 16S27F (5'-AGAGTTTGATCMTGGCTCAG-3') and reverse primer r1492 (5'-TACGGYTACCTTGTTACGACTT-3'), following the protocol described in Castiglioni et al. [ ] except for using 50 ng of starting DNA and 0.5 U of DNAzyme DNA polymerase II (Finnzymes, Espoo, Finland).

Article Title: First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax
Article Snippet: Paragraph title: DNA extraction, PCR amplification, and sequence analysis ... PCR products were purified using a PCR Clean-up System purification kit (Promega, Madison, WI, USA) and sequenced using BigDye terminator cycle sequence kits (Applied Biosystems) with a ABI Prism 310 genetic analyzer (Applied Biosystems).

Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
Article Snippet: Paragraph title: 2.3. DNA Extraction and PCR Amplification ... The PCR products were purified by using a Wizard SV gel and PCR Clean-Up System purification kit (Promega Italia, Milan, Italy) and quantified by electrophoresis in agarose gel with the quantitative LowRanger 100 bp DNA Ladder (Norgen Biotek Corp., Thorold, Ontario, Canada).

Agarose Gel Electrophoresis:

Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
Article Snippet: .. The PCR products were purified by using a Wizard SV gel and PCR Clean-Up System purification kit (Promega Italia, Milan, Italy) and quantified by electrophoresis in agarose gel with the quantitative LowRanger 100 bp DNA Ladder (Norgen Biotek Corp., Thorold, Ontario, Canada). .. Ligase Detection Reaction (LDR) Selected probes were ordered for synthesis (Thermo Fisher Scientific GmbH, Ulm, Germany).

Synthesized:

Article Title: High taxonomic level fingerprint of the human intestinal microbiota by Ligase Detection Reaction - Universal Array approach
Article Snippet: Polymerase Chain Reaction (PCR) All the oligonucleotide primers and probe pairs were synthesized by Thermo Electron (Ulm, Germany). .. PCR products were purified by using a Wizard SV gel and PCR clean-up System purification kit (Promega Italia, Milan, Italy), according to the manufacturer's instructions, eluted in 20 μl of sterile water, and quantified with the DNA 7500 LabChip Assay kit and BioAnalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA).

Isolation:

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: RT-PCR and VP1 sequencing In Sri Lanka, we sequenced the VP1 locus of the FMDV isolated from 10 tissue samples from the 2014 outbreak and two samples from the reported cases in 2012. .. The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA).

Purification:

Article Title: High taxonomic level fingerprint of the human intestinal microbiota by Ligase Detection Reaction - Universal Array approach
Article Snippet: .. PCR products were purified by using a Wizard SV gel and PCR clean-up System purification kit (Promega Italia, Milan, Italy), according to the manufacturer's instructions, eluted in 20 μl of sterile water, and quantified with the DNA 7500 LabChip Assay kit and BioAnalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). .. 16S rRNA was amplified using universal forward primer 16S27F (5'-AGAGTTTGATCMTGGCTCAG-3') and reverse primer r1492 (5'-TACGGYTACCTTGTTACGACTT-3'), following the protocol described in Castiglioni et al. [ ] except for using 50 ng of starting DNA and 0.5 U of DNAzyme DNA polymerase II (Finnzymes, Espoo, Finland).

Article Title: First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax
Article Snippet: .. PCR products were purified using a PCR Clean-up System purification kit (Promega, Madison, WI, USA) and sequenced using BigDye terminator cycle sequence kits (Applied Biosystems) with a ABI Prism 310 genetic analyzer (Applied Biosystems). ..

Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
Article Snippet: .. The PCR products were purified by using a Wizard SV gel and PCR Clean-Up System purification kit (Promega Italia, Milan, Italy) and quantified by electrophoresis in agarose gel with the quantitative LowRanger 100 bp DNA Ladder (Norgen Biotek Corp., Thorold, Ontario, Canada). .. Ligase Detection Reaction (LDR) Selected probes were ordered for synthesis (Thermo Fisher Scientific GmbH, Ulm, Germany).

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: .. The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA). .. The purified PCR products were subjected to triplicate DNA sequencing using ABI 3730 DNA Analyzer according to the protocol described in Knowles et al ., 2016 [ ].

Article Title: Polymorphism in the HASPB Repeat Region of East African Leishmania donovani Strains
Article Snippet: .. DNA sequencing and analysis For direct sequencing, the PCR products were purified using Wizard SV gel and PCR clean-up system purification kit (Promega, WI, USA). ..

Electrophoresis:

Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
Article Snippet: .. The PCR products were purified by using a Wizard SV gel and PCR Clean-Up System purification kit (Promega Italia, Milan, Italy) and quantified by electrophoresis in agarose gel with the quantitative LowRanger 100 bp DNA Ladder (Norgen Biotek Corp., Thorold, Ontario, Canada). .. Ligase Detection Reaction (LDR) Selected probes were ordered for synthesis (Thermo Fisher Scientific GmbH, Ulm, Germany).

Polymerase Chain Reaction:

Article Title: High taxonomic level fingerprint of the human intestinal microbiota by Ligase Detection Reaction - Universal Array approach
Article Snippet: .. PCR products were purified by using a Wizard SV gel and PCR clean-up System purification kit (Promega Italia, Milan, Italy), according to the manufacturer's instructions, eluted in 20 μl of sterile water, and quantified with the DNA 7500 LabChip Assay kit and BioAnalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA). .. 16S rRNA was amplified using universal forward primer 16S27F (5'-AGAGTTTGATCMTGGCTCAG-3') and reverse primer r1492 (5'-TACGGYTACCTTGTTACGACTT-3'), following the protocol described in Castiglioni et al. [ ] except for using 50 ng of starting DNA and 0.5 U of DNAzyme DNA polymerase II (Finnzymes, Espoo, Finland).

Article Title: First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax
Article Snippet: .. PCR products were purified using a PCR Clean-up System purification kit (Promega, Madison, WI, USA) and sequenced using BigDye terminator cycle sequence kits (Applied Biosystems) with a ABI Prism 310 genetic analyzer (Applied Biosystems). ..

Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes
Article Snippet: .. The PCR products were purified by using a Wizard SV gel and PCR Clean-Up System purification kit (Promega Italia, Milan, Italy) and quantified by electrophoresis in agarose gel with the quantitative LowRanger 100 bp DNA Ladder (Norgen Biotek Corp., Thorold, Ontario, Canada). .. Ligase Detection Reaction (LDR) Selected probes were ordered for synthesis (Thermo Fisher Scientific GmbH, Ulm, Germany).

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: .. The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA). .. The purified PCR products were subjected to triplicate DNA sequencing using ABI 3730 DNA Analyzer according to the protocol described in Knowles et al ., 2016 [ ].

Article Title: Polymorphism in the HASPB Repeat Region of East African Leishmania donovani Strains
Article Snippet: .. DNA sequencing and analysis For direct sequencing, the PCR products were purified using Wizard SV gel and PCR clean-up system purification kit (Promega, WI, USA). ..

Generated:

Article Title: First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax
Article Snippet: PCR products were purified using a PCR Clean-up System purification kit (Promega, Madison, WI, USA) and sequenced using BigDye terminator cycle sequence kits (Applied Biosystems) with a ABI Prism 310 genetic analyzer (Applied Biosystems). .. Sequences generated from the materials in this study and those retrieved from GenBank were initially aligned using the CLUSTAL X program [ ], and the alignment was refined using PHYDIT program ver.

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA). .. We submitted the 53 VP1 sequences generated in this study to GenBank under the accession numbers; MF947453—MF947493 and MF768987—MF768998.

DNA Sequencing:

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA). .. The purified PCR products were subjected to triplicate DNA sequencing using ABI 3730 DNA Analyzer according to the protocol described in Knowles et al ., 2016 [ ].

Article Title: Polymorphism in the HASPB Repeat Region of East African Leishmania donovani Strains
Article Snippet: .. DNA sequencing and analysis For direct sequencing, the PCR products were purified using Wizard SV gel and PCR clean-up system purification kit (Promega, WI, USA). ..

Sequencing:

Article Title: First Report of Foliar Blight on Dendropanax morbifera Caused by Alternaria panax
Article Snippet: .. PCR products were purified using a PCR Clean-up System purification kit (Promega, Madison, WI, USA) and sequenced using BigDye terminator cycle sequence kits (Applied Biosystems) with a ABI Prism 310 genetic analyzer (Applied Biosystems). ..

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: Paragraph title: RT-PCR and VP1 sequencing ... The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA).

Article Title: Polymorphism in the HASPB Repeat Region of East African Leishmania donovani Strains
Article Snippet: .. DNA sequencing and analysis For direct sequencing, the PCR products were purified using Wizard SV gel and PCR clean-up system purification kit (Promega, WI, USA). ..

Reverse Transcription Polymerase Chain Reaction:

Article Title: The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage
Article Snippet: Paragraph title: RT-PCR and VP1 sequencing ... The PCR products were purified using Promega gel and PCR clean-up system purification kit (Promega, Madison, USA).

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    Promega pcr clean up system purification kit
    Pcr Clean Up System Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up system purification kit/product/Promega
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr clean up system purification kit - by Bioz Stars, 2020-04
    93/100 stars
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