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Promega pcr clean up purification kits
Pcr Clean Up Purification Kits, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean up purification kits/product/Promega
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pcr clean up purification kits - by Bioz Stars, 2020-03
92/100 stars

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Countercurrent Chromatography:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: The primer pairs were constructed as follows: NV-GII.4-RdRp-F 5′- TGY CCC TAY ATC TAC AAG AG-3′ and NV-GII.4-VP1-R 5′- TCA ATT TGT GCT TGG AGC AT-3′, which corresponded to positions 3449–6912 according to the genome of the NV Hu/GII-4/Saga1/2006/JP strain (GenBank accession number, AB447456 ). .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS).

Amplification:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: The genome fragment was amplified using RT-PCR and SuperScript III RT/Platinum Taq High Fidelity Enzyme Mix (Thermo Fischer Scientific), according to the standard protocol. .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS).

Polymerase Chain Reaction:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS). .. The library of the amplicons was prepared using Nextera XT DNA Library Preparation kits (Illumina, San Diego, CA, USA), following the manufacturer instructions, which generated 300 bp fragments.

Next-Generation Sequencing:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS). .. The library of the amplicons was prepared using Nextera XT DNA Library Preparation kits (Illumina, San Diego, CA, USA), following the manufacturer instructions, which generated 300 bp fragments.

Construct:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: The primer pairs were constructed as follows: NV-GII.4-RdRp-F 5′- TGY CCC TAY ATC TAC AAG AG-3′ and NV-GII.4-VP1-R 5′- TCA ATT TGT GCT TGG AGC AT-3′, which corresponded to positions 3449–6912 according to the genome of the NV Hu/GII-4/Saga1/2006/JP strain (GenBank accession number, AB447456 ). .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS).

Purification:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS). .. The library of the amplicons was prepared using Nextera XT DNA Library Preparation kits (Illumina, San Diego, CA, USA), following the manufacturer instructions, which generated 300 bp fragments.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: The genome fragment was amplified using RT-PCR and SuperScript III RT/Platinum Taq High Fidelity Enzyme Mix (Thermo Fischer Scientific), according to the standard protocol. .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS).

Generated:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS). .. The library of the amplicons was prepared using Nextera XT DNA Library Preparation kits (Illumina, San Diego, CA, USA), following the manufacturer instructions, which generated 300 bp fragments.

Infection:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: Generation of amplicons and next-generation sequencing The NV genome amplicon for virus population studies in chronic infected hosts was obtained with primer-pair construction that allowed amplification of the total RdRp and VP1 genes. .. The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS).

Sequencing:

Article Title: Intrahost Norovirus Evolution in Chronic Infection Over 5 Years of Shedding in a Kidney Transplant Recipient
Article Snippet: The PCR products were purified using Wizard SV Gels and PCR Clean-up purification kits (Promega, Madison, WI, USA), and they were stored at −20°C until the library preparation and next-generation sequencing (NGS). .. The sequencing was performed on MiSeq (Illumina), generating paired-end reads.

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    Promega pcr clean up purification kits
    Pcr Clean Up Purification Kits, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up purification kits/product/Promega
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr clean up purification kits - by Bioz Stars, 2020-03
    92/100 stars
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