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MACHEREY NAGEL pcr clean up purification kit
Pcr Clean Up Purification Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean up purification kit/product/MACHEREY NAGEL
Average 90 stars, based on 9 article reviews
Price from $9.99 to $1999.99
pcr clean up purification kit - by Bioz Stars, 2020-07
90/100 stars

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Nucleic Acid Electrophoresis:

Article Title: In Vitro and in Vivo Selection of Potentially Probiotic Lactobacilli From Nocellara del Belice Table Olives
Article Snippet: .. Two μl of DNA extracted by microlysis were used for PCR amplification with the following program: 95°C for 10 min, 30 cycles at: 94°C for 1 min, 55°C for 90 s, 72°C for 150 s; one step at 55°C for 10 min followed by a final step at 72°C for 10 min. Amplified PCR fragments were analyzed by gel electrophoresis in 0.8% agarose in 1X TAE and then purified with NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany). .. Sequencing of purified 16S rDNA fragments was performed at Bio-Fab Research (Italy) laboratories.

Amplification:

Article Title: Fruit fracture biomechanics and the release of Lepidium didymum pericarp-imposed mechanical dormancy by fungi
Article Snippet: .. Amplification products were purified using the Nucleospin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), ligated into plasmid vector pGEM-T (Promega, Mannheim, Germany) and transformed into E. coli strain DH5α. .. Plasmid DNA containing inserts were isolated from bacterial colonies and the PCR products were sequenced on an ABI Prism 377 automated sequencer with dye terminator chemistry (Life Technologies, Darmstadt, Germany).

Article Title: In Vitro and in Vivo Selection of Potentially Probiotic Lactobacilli From Nocellara del Belice Table Olives
Article Snippet: .. Two μl of DNA extracted by microlysis were used for PCR amplification with the following program: 95°C for 10 min, 30 cycles at: 94°C for 1 min, 55°C for 90 s, 72°C for 150 s; one step at 55°C for 10 min followed by a final step at 72°C for 10 min. Amplified PCR fragments were analyzed by gel electrophoresis in 0.8% agarose in 1X TAE and then purified with NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany). .. Sequencing of purified 16S rDNA fragments was performed at Bio-Fab Research (Italy) laboratories.

Agarose Gel Electrophoresis:

Article Title: Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells
Article Snippet: .. The PCR products were loaded on a 1.5% agarose gel, and the bands were purified using a NucleoSpin gel and PCR Clean-up Purification Kit, according to the manufacturer’s recommendations (Macherey-Nagel). .. The purified PCR products (160 ng) were digested with BamHI and SalI using BamHI buffer for 1.5 h at 37°C.

Picogreen Assay:

Article Title: Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes
Article Snippet: .. Amplicons from seven of the positive PCR products were purified using the NucleoSpin Gel and PCR clean-up purification kit as recommended by the manufacturer (Macherey-Nagel, Germany) and quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for Sanger sequencing identification according to specifications at the Uppsala Genome Center. ..

Article Title: Genomes from uncultivated prokaryotes: a comparison of metagenome-assembled and single-amplified genomes
Article Snippet: .. The products were purified using the NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany), quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA, USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for identification by Sanger sequencing at the Uppsala Genome Center. .. Illumina MiSeq sequencing Altogether, 15 SAGs were selected for genome sequencing.

Purification:

Article Title: Fruit fracture biomechanics and the release of Lepidium didymum pericarp-imposed mechanical dormancy by fungi
Article Snippet: .. Amplification products were purified using the Nucleospin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), ligated into plasmid vector pGEM-T (Promega, Mannheim, Germany) and transformed into E. coli strain DH5α. .. Plasmid DNA containing inserts were isolated from bacterial colonies and the PCR products were sequenced on an ABI Prism 377 automated sequencer with dye terminator chemistry (Life Technologies, Darmstadt, Germany).

Article Title: Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells
Article Snippet: .. The digested plasmid was gel purified using a NucleoSpin Gel and PCR Clean-up purification kit, according to the manufacturer’s recommendations (Macherey-Nagel). ..

Article Title: Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes
Article Snippet: .. Amplicons from seven of the positive PCR products were purified using the NucleoSpin Gel and PCR clean-up purification kit as recommended by the manufacturer (Macherey-Nagel, Germany) and quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for Sanger sequencing identification according to specifications at the Uppsala Genome Center. ..

Article Title: Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks
Article Snippet: .. To further support the specificity of our approach, the PCR products, after gel purification by NucleoSpin gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), were double strand sequenced ( https://www.gatc-biotech.com ). ..

Article Title: Genomes from uncultivated prokaryotes: a comparison of metagenome-assembled and single-amplified genomes
Article Snippet: .. The products were purified using the NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany), quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA, USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for identification by Sanger sequencing at the Uppsala Genome Center. .. Illumina MiSeq sequencing Altogether, 15 SAGs were selected for genome sequencing.

Article Title: In Vitro and in Vivo Selection of Potentially Probiotic Lactobacilli From Nocellara del Belice Table Olives
Article Snippet: .. Two μl of DNA extracted by microlysis were used for PCR amplification with the following program: 95°C for 10 min, 30 cycles at: 94°C for 1 min, 55°C for 90 s, 72°C for 150 s; one step at 55°C for 10 min followed by a final step at 72°C for 10 min. Amplified PCR fragments were analyzed by gel electrophoresis in 0.8% agarose in 1X TAE and then purified with NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany). .. Sequencing of purified 16S rDNA fragments was performed at Bio-Fab Research (Italy) laboratories.

Article Title: Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells
Article Snippet: .. The PCR products were loaded on a 1.5% agarose gel, and the bands were purified using a NucleoSpin gel and PCR Clean-up Purification Kit, according to the manufacturer’s recommendations (Macherey-Nagel). .. The purified PCR products (160 ng) were digested with BamHI and SalI using BamHI buffer for 1.5 h at 37°C.

Polymerase Chain Reaction:

Article Title: Fruit fracture biomechanics and the release of Lepidium didymum pericarp-imposed mechanical dormancy by fungi
Article Snippet: .. Amplification products were purified using the Nucleospin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), ligated into plasmid vector pGEM-T (Promega, Mannheim, Germany) and transformed into E. coli strain DH5α. .. Plasmid DNA containing inserts were isolated from bacterial colonies and the PCR products were sequenced on an ABI Prism 377 automated sequencer with dye terminator chemistry (Life Technologies, Darmstadt, Germany).

Article Title: Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells
Article Snippet: .. The digested plasmid was gel purified using a NucleoSpin Gel and PCR Clean-up purification kit, according to the manufacturer’s recommendations (Macherey-Nagel). ..

Article Title: Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes
Article Snippet: .. Amplicons from seven of the positive PCR products were purified using the NucleoSpin Gel and PCR clean-up purification kit as recommended by the manufacturer (Macherey-Nagel, Germany) and quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for Sanger sequencing identification according to specifications at the Uppsala Genome Center. ..

Article Title: Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks
Article Snippet: .. To further support the specificity of our approach, the PCR products, after gel purification by NucleoSpin gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), were double strand sequenced ( https://www.gatc-biotech.com ). ..

Article Title: Genomes from uncultivated prokaryotes: a comparison of metagenome-assembled and single-amplified genomes
Article Snippet: .. The products were purified using the NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany), quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA, USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for identification by Sanger sequencing at the Uppsala Genome Center. .. Illumina MiSeq sequencing Altogether, 15 SAGs were selected for genome sequencing.

Article Title: In Vitro and in Vivo Selection of Potentially Probiotic Lactobacilli From Nocellara del Belice Table Olives
Article Snippet: .. Two μl of DNA extracted by microlysis were used for PCR amplification with the following program: 95°C for 10 min, 30 cycles at: 94°C for 1 min, 55°C for 90 s, 72°C for 150 s; one step at 55°C for 10 min followed by a final step at 72°C for 10 min. Amplified PCR fragments were analyzed by gel electrophoresis in 0.8% agarose in 1X TAE and then purified with NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany). .. Sequencing of purified 16S rDNA fragments was performed at Bio-Fab Research (Italy) laboratories.

Article Title: Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells
Article Snippet: .. The PCR products were loaded on a 1.5% agarose gel, and the bands were purified using a NucleoSpin gel and PCR Clean-up Purification Kit, according to the manufacturer’s recommendations (Macherey-Nagel). .. The purified PCR products (160 ng) were digested with BamHI and SalI using BamHI buffer for 1.5 h at 37°C.

Sequencing:

Article Title: Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes
Article Snippet: .. Amplicons from seven of the positive PCR products were purified using the NucleoSpin Gel and PCR clean-up purification kit as recommended by the manufacturer (Macherey-Nagel, Germany) and quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for Sanger sequencing identification according to specifications at the Uppsala Genome Center. ..

Article Title: Genomes from uncultivated prokaryotes: a comparison of metagenome-assembled and single-amplified genomes
Article Snippet: .. The products were purified using the NucleoSpin Gel and PCR clean-up purification kit (Macherey-Nagel, Germany), quantified using the Quant-iT ™ PicoGreen® dsDNA assay kit (Invitrogen, MA, USA) in a FLUOstar® Omega microplate reader (BMG Labtech, Germany) and submitted for identification by Sanger sequencing at the Uppsala Genome Center. .. Illumina MiSeq sequencing Altogether, 15 SAGs were selected for genome sequencing.

Transformation Assay:

Article Title: Fruit fracture biomechanics and the release of Lepidium didymum pericarp-imposed mechanical dormancy by fungi
Article Snippet: .. Amplification products were purified using the Nucleospin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), ligated into plasmid vector pGEM-T (Promega, Mannheim, Germany) and transformed into E. coli strain DH5α. .. Plasmid DNA containing inserts were isolated from bacterial colonies and the PCR products were sequenced on an ABI Prism 377 automated sequencer with dye terminator chemistry (Life Technologies, Darmstadt, Germany).

Plasmid Preparation:

Article Title: Fruit fracture biomechanics and the release of Lepidium didymum pericarp-imposed mechanical dormancy by fungi
Article Snippet: .. Amplification products were purified using the Nucleospin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), ligated into plasmid vector pGEM-T (Promega, Mannheim, Germany) and transformed into E. coli strain DH5α. .. Plasmid DNA containing inserts were isolated from bacterial colonies and the PCR products were sequenced on an ABI Prism 377 automated sequencer with dye terminator chemistry (Life Technologies, Darmstadt, Germany).

Article Title: Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells
Article Snippet: .. The digested plasmid was gel purified using a NucleoSpin Gel and PCR Clean-up purification kit, according to the manufacturer’s recommendations (Macherey-Nagel). ..

Gel Purification:

Article Title: Paternal leakage and mtDNA heteroplasmy in Rhipicephalus spp. ticks
Article Snippet: .. To further support the specificity of our approach, the PCR products, after gel purification by NucleoSpin gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany), were double strand sequenced ( https://www.gatc-biotech.com ). ..

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    MACHEREY NAGEL pcr clean up purification kit
    Pcr Clean Up Purification Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up purification kit/product/MACHEREY NAGEL
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr clean up purification kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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