Structured Review

Stratagene pcr based quikchangetm strategy
Pcr Based Quikchangetm Strategy, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr based quikchangetm strategy/product/Stratagene
Average 86 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pcr based quikchangetm strategy - by Bioz Stars, 2020-07
86/100 stars

Images

Related Articles

Cell Based Assay:

Article Title: Binding of longer A? to transmembrane domain 1 of presenilin 1 impacts on A?42 generation
Article Snippet: .. Plasmid construction, cell culture manipulation and cell based assay cDNAs encoding PS1 and APP carrying Swedish mutation (APPNL) were inserted into pMXs-puro [ ]. cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. To produce recombinant proteins, cDNAs encoding PS1 were cloned into pGEX-6P-1 vector (GE healthcare) [ ].

Mutagenesis:

Article Title: Binding of longer A? to transmembrane domain 1 of presenilin 1 impacts on A?42 generation
Article Snippet: .. Plasmid construction, cell culture manipulation and cell based assay cDNAs encoding PS1 and APP carrying Swedish mutation (APPNL) were inserted into pMXs-puro [ ]. cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. To produce recombinant proteins, cDNAs encoding PS1 were cloned into pGEX-6P-1 vector (GE healthcare) [ ].

Article Title: Functional Analysis of the Transmembrane Domains of Presenilin 1
Article Snippet: .. cDNAs encoding PS1, APP carrying Swedish mutation (APPNL ), and NotchΔE were inserted into pMXs-puro ( , , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. All constructs were sequenced using Thermo Sequenase (USB Corp., Cleveland, OH) on an automated sequencer (Li-Cor, Lincoln, NE) (see ).

Article Title: Contribution of the ?-Secretase Subunits to the Formation of Catalytic Pore of Presenilin 1 Protein *
Article Snippet: .. For expression in mammalian cells, cDNAs encoding human PS1 mutants were inserted into pMXs-puro ( – , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. For the expression of γ-secretase subunits in Sf9 cells, PS1 mutants and Aph-1aL-Myc/His were inserted into pFastBac Dual (Invitrogen).

Cell Culture:

Article Title: Binding of longer A? to transmembrane domain 1 of presenilin 1 impacts on A?42 generation
Article Snippet: .. Plasmid construction, cell culture manipulation and cell based assay cDNAs encoding PS1 and APP carrying Swedish mutation (APPNL) were inserted into pMXs-puro [ ]. cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. To produce recombinant proteins, cDNAs encoding PS1 were cloned into pGEX-6P-1 vector (GE healthcare) [ ].

Generated:

Article Title: Binding of longer A? to transmembrane domain 1 of presenilin 1 impacts on A?42 generation
Article Snippet: .. Plasmid construction, cell culture manipulation and cell based assay cDNAs encoding PS1 and APP carrying Swedish mutation (APPNL) were inserted into pMXs-puro [ ]. cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. To produce recombinant proteins, cDNAs encoding PS1 were cloned into pGEX-6P-1 vector (GE healthcare) [ ].

Article Title: Functional Analysis of the Transmembrane Domains of Presenilin 1
Article Snippet: .. cDNAs encoding PS1, APP carrying Swedish mutation (APPNL ), and NotchΔE were inserted into pMXs-puro ( , , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. All constructs were sequenced using Thermo Sequenase (USB Corp., Cleveland, OH) on an automated sequencer (Li-Cor, Lincoln, NE) (see ).

Article Title: Contribution of the ?-Secretase Subunits to the Formation of Catalytic Pore of Presenilin 1 Protein *
Article Snippet: .. For expression in mammalian cells, cDNAs encoding human PS1 mutants were inserted into pMXs-puro ( – , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. For the expression of γ-secretase subunits in Sf9 cells, PS1 mutants and Aph-1aL-Myc/His were inserted into pFastBac Dual (Invitrogen).

Expressing:

Article Title: Contribution of the ?-Secretase Subunits to the Formation of Catalytic Pore of Presenilin 1 Protein *
Article Snippet: .. For expression in mammalian cells, cDNAs encoding human PS1 mutants were inserted into pMXs-puro ( – , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. For the expression of γ-secretase subunits in Sf9 cells, PS1 mutants and Aph-1aL-Myc/His were inserted into pFastBac Dual (Invitrogen).

Polymerase Chain Reaction:

Article Title: Binding of longer A? to transmembrane domain 1 of presenilin 1 impacts on A?42 generation
Article Snippet: .. Plasmid construction, cell culture manipulation and cell based assay cDNAs encoding PS1 and APP carrying Swedish mutation (APPNL) were inserted into pMXs-puro [ ]. cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. To produce recombinant proteins, cDNAs encoding PS1 were cloned into pGEX-6P-1 vector (GE healthcare) [ ].

Article Title: Functional Analysis of the Transmembrane Domains of Presenilin 1
Article Snippet: .. cDNAs encoding PS1, APP carrying Swedish mutation (APPNL ), and NotchΔE were inserted into pMXs-puro ( , , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. All constructs were sequenced using Thermo Sequenase (USB Corp., Cleveland, OH) on an automated sequencer (Li-Cor, Lincoln, NE) (see ).

Article Title: Contribution of the ?-Secretase Subunits to the Formation of Catalytic Pore of Presenilin 1 Protein *
Article Snippet: .. For expression in mammalian cells, cDNAs encoding human PS1 mutants were inserted into pMXs-puro ( – , ). cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. For the expression of γ-secretase subunits in Sf9 cells, PS1 mutants and Aph-1aL-Myc/His were inserted into pFastBac Dual (Invitrogen).

Plasmid Preparation:

Article Title: Binding of longer A? to transmembrane domain 1 of presenilin 1 impacts on A?42 generation
Article Snippet: .. Plasmid construction, cell culture manipulation and cell based assay cDNAs encoding PS1 and APP carrying Swedish mutation (APPNL) were inserted into pMXs-puro [ ]. cDNAs encoding mutant PS1 were generated by long PCR-based QuikChangeTM strategy (Stratagene). .. To produce recombinant proteins, cDNAs encoding PS1 were cloned into pGEX-6P-1 vector (GE healthcare) [ ].

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Stratagene pcr based quikchangetm strategy
    Pcr Based Quikchangetm Strategy, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr based quikchangetm strategy/product/Stratagene
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr based quikchangetm strategy - by Bioz Stars, 2020-07
    86/100 stars
      Buy from Supplier

    Image Search Results