pcr based quikchange site directed mutagenesis kit  (Stratagene)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89

    Structured Review

    Stratagene pcr based quikchange site directed mutagenesis kit
    Pcr Based Quikchange Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr based quikchange site directed mutagenesis kit/product/Stratagene
    Average 89 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    pcr based quikchange site directed mutagenesis kit - by Bioz Stars, 2020-09
    89/100 stars

    Images

    Related Articles

    Mutagenesis:

    Article Title: Ring Finger Protein 149 Is an E3 Ubiquitin Ligase Active on Wild-type v-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) *
    Article Snippet: .. The mutant constructs BRAFV600E and RNF149H289A , in which Val-600 was replaced with glutamine and His-289 was replaced with alanine, respectively, were created using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene). .. Lipofectamine 2000 (Invitrogen) was used for transient transfections.

    Article Title: The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding FunctionsGene Sharing Yields an Enzyme with Two Binding Sites in One Subunit
    Article Snippet: .. Site-directed mutagenesis was carried out using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer's instructions, using pCtCE2 or pCjCE2A as the template and primers pairs that are listed in . ..

    Article Title: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis
    Article Snippet: .. Mutagenesis of golgin-160 was carried out using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene), and the mutations were confirmed by dideoxy sequencing. .. In Vitro Cleavage of [35 S]Methionine-labeled Substrates Generated by In Vitro Transcription/Translation [35 S]methionine-labeled golgin-160 and golgin-160 mutants were generated by coupled in vitro transcription/translation ( ).

    Article Title: The Structure and Function of an Arabinan-specific ?-1,2-Arabinofuranosidase Identified from Screening the Activities of Bacterial GH43 Glycoside Hydrolases *
    Article Snippet: .. Site-directed mutagenesis was conducted using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer's instructions, using pAC12 as the template and the primer pairs listed in . .. The substrates 4-nitrophenyl-α- l -arabinofuranoside (4NPA) and sugar beet arabinan were purchased from Sigma and Megazyme, respectively.

    Article Title: Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-?-d-xylanase 10B in complex with xylohexaose
    Article Snippet: .. The CBM22-1–GH10 nucleophile mutant (Glu→Ala), termed CBM22-1–GH10 E337A, was generated using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. .. The primers used to generate the nucleic acid mutant were GACGTTGTAAAT GCG GCAGTAAGTGATGATGC and GCATCATCACTTACTGC CGC ATTTACAACGTC (nucleotide substitutions in bold).

    Article Title: Modulation of Endogenous Indole-3-Acetic Acid Biosynthesis in Bacteroids within Medicago sativa Nodules
    Article Snippet: .. Site-directed mutagenesis of the promintron consensus sequences was performed in two steps by using a PCR-based QuikChange site-directed mutagenesis kit (Stratagene). ..

    Article Title: Nuclear Factor Erythroid 2-like 2 Regulates Expression of Inositol 1,4,5-trisphosphate Receptor, Type 3 and Calcium Signaling in Cholangiocytes
    Article Snippet: .. The MARE reporter construct was mutated using the PCR-based QuikChange™ site-directed mutagenesis kit (Stratagene; Cedar creek, TX). ..

    Polymerase Chain Reaction:

    Article Title: Ring Finger Protein 149 Is an E3 Ubiquitin Ligase Active on Wild-type v-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) *
    Article Snippet: .. The mutant constructs BRAFV600E and RNF149H289A , in which Val-600 was replaced with glutamine and His-289 was replaced with alanine, respectively, were created using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene). .. Lipofectamine 2000 (Invitrogen) was used for transient transfections.

    Article Title: The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding FunctionsGene Sharing Yields an Enzyme with Two Binding Sites in One Subunit
    Article Snippet: .. Site-directed mutagenesis was carried out using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer's instructions, using pCtCE2 or pCjCE2A as the template and primers pairs that are listed in . ..

    Article Title: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis
    Article Snippet: .. Mutagenesis of golgin-160 was carried out using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene), and the mutations were confirmed by dideoxy sequencing. .. In Vitro Cleavage of [35 S]Methionine-labeled Substrates Generated by In Vitro Transcription/Translation [35 S]methionine-labeled golgin-160 and golgin-160 mutants were generated by coupled in vitro transcription/translation ( ).

    Article Title: The Structure and Function of an Arabinan-specific ?-1,2-Arabinofuranosidase Identified from Screening the Activities of Bacterial GH43 Glycoside Hydrolases *
    Article Snippet: .. Site-directed mutagenesis was conducted using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer's instructions, using pAC12 as the template and the primer pairs listed in . .. The substrates 4-nitrophenyl-α- l -arabinofuranoside (4NPA) and sugar beet arabinan were purchased from Sigma and Megazyme, respectively.

    Article Title: Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-?-d-xylanase 10B in complex with xylohexaose
    Article Snippet: .. The CBM22-1–GH10 nucleophile mutant (Glu→Ala), termed CBM22-1–GH10 E337A, was generated using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. .. The primers used to generate the nucleic acid mutant were GACGTTGTAAAT GCG GCAGTAAGTGATGATGC and GCATCATCACTTACTGC CGC ATTTACAACGTC (nucleotide substitutions in bold).

    Article Title: Modulation of Endogenous Indole-3-Acetic Acid Biosynthesis in Bacteroids within Medicago sativa Nodules
    Article Snippet: .. Site-directed mutagenesis of the promintron consensus sequences was performed in two steps by using a PCR-based QuikChange site-directed mutagenesis kit (Stratagene). ..

    Article Title: Nuclear Factor Erythroid 2-like 2 Regulates Expression of Inositol 1,4,5-trisphosphate Receptor, Type 3 and Calcium Signaling in Cholangiocytes
    Article Snippet: .. The MARE reporter construct was mutated using the PCR-based QuikChange™ site-directed mutagenesis kit (Stratagene; Cedar creek, TX). ..

    Generated:

    Article Title: Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-?-d-xylanase 10B in complex with xylohexaose
    Article Snippet: .. The CBM22-1–GH10 nucleophile mutant (Glu→Ala), termed CBM22-1–GH10 E337A, was generated using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. .. The primers used to generate the nucleic acid mutant were GACGTTGTAAAT GCG GCAGTAAGTGATGATGC and GCATCATCACTTACTGC CGC ATTTACAACGTC (nucleotide substitutions in bold).

    Article Title: Mutational Analysis Reveals Distinct Features of the Nox4-p22phox Complex * Complex * S⃞
    Article Snippet: .. Amino acid exchange mutants W6/9F, W6/9A, W6/9R, W6/9D, H72H, R90Q, P156Q, and Y121H and various lysine exchange mutants in regions aa 32-35 and aa 98-102 were generated using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer's protocol. .. Mouse Nox4 was a gift of Dr. B. Banfi; mouse p22phox was cloned from mouse macrophages.

    Sequencing:

    Article Title: Caspase-2 Is Localized at the Golgi Complex and Cleaves Golgin-160 during Apoptosis
    Article Snippet: .. Mutagenesis of golgin-160 was carried out using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene), and the mutations were confirmed by dideoxy sequencing. .. In Vitro Cleavage of [35 S]Methionine-labeled Substrates Generated by In Vitro Transcription/Translation [35 S]methionine-labeled golgin-160 and golgin-160 mutants were generated by coupled in vitro transcription/translation ( ).

    Construct:

    Article Title: Ring Finger Protein 149 Is an E3 Ubiquitin Ligase Active on Wild-type v-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) *
    Article Snippet: .. The mutant constructs BRAFV600E and RNF149H289A , in which Val-600 was replaced with glutamine and His-289 was replaced with alanine, respectively, were created using the PCR-based QuikChange site-directed mutagenesis kit (Stratagene). .. Lipofectamine 2000 (Invitrogen) was used for transient transfections.

    Article Title: Nuclear Factor Erythroid 2-like 2 Regulates Expression of Inositol 1,4,5-trisphosphate Receptor, Type 3 and Calcium Signaling in Cholangiocytes
    Article Snippet: .. The MARE reporter construct was mutated using the PCR-based QuikChange™ site-directed mutagenesis kit (Stratagene; Cedar creek, TX). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Stratagene pcr based site directed mutagenesis the quikchange site directed mutagenesis kit stratagene la jolla ca
    Pcr Based Site Directed Mutagenesis The Quikchange Site Directed Mutagenesis Kit Stratagene La Jolla Ca, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr based site directed mutagenesis the quikchange site directed mutagenesis kit stratagene la jolla ca/product/Stratagene
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcr based site directed mutagenesis the quikchange site directed mutagenesis kit stratagene la jolla ca - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    88
    Stratagene pcr based quikchange ii xl site directed mutagenesis kit
    Pcr Based Quikchange Ii Xl Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr based quikchange ii xl site directed mutagenesis kit/product/Stratagene
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pcr based quikchange ii xl site directed mutagenesis kit - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    94
    Stratagene quikchange lightning site directed mutagenesis kit
    Quikchange Lightning Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange lightning site directed mutagenesis kit/product/Stratagene
    Average 94 stars, based on 489 article reviews
    Price from $9.99 to $1999.99
    quikchange lightning site directed mutagenesis kit - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    95
    Stratagene quikchange site directed mutagenesis kit
    Quikchange Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 95/100, based on 3351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange site directed mutagenesis kit/product/Stratagene
    Average 95 stars, based on 3351 article reviews
    Price from $9.99 to $1999.99
    quikchange site directed mutagenesis kit - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results