pcr based mutagenesis strategy  (New England Biolabs)


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    Name:
    Q5 Site Directed Mutagenesis Kit Without Competent Cells
    Description:
    Q5 Site Directed Mutagenesis Kit Without Competent Cells 10 rxns
    Catalog Number:
    e0552s
    Price:
    137
    Size:
    10 rxns
    Category:
    PCR Mutagenesis Kits
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    New England Biolabs pcr based mutagenesis strategy
    Q5 Site Directed Mutagenesis Kit Without Competent Cells
    Q5 Site Directed Mutagenesis Kit Without Competent Cells 10 rxns
    https://www.bioz.com/result/pcr based mutagenesis strategy/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr based mutagenesis strategy - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Positron Emission Tomography:

    Article Title: Structure and mechanism of a bacterial t6A biosynthesis system
    Article Snippet: .. Single-site mutagenesis of T. maritima TsaE Point mutagenesis was carried out on TsaE (cloned in pET-28a) using the Q5® Site-Directed Mutagenesis Kit (NEW ENGLAND Biolabs Inc.). ..

    Mutagenesis:

    Article Title: ADAMTS-10 and -6 differentially regulate cell-cell junctions and focal adhesions
    Article Snippet: .. Mutations were created using Q5® Site-Directed Mutagenesis Kit (NEB), and fully sequenced before use. .. HEK293T cells were co-transfected with psPax2, pMD2.G packaging as well as a target pCDH-EF1-T2A-copGFP vector.

    Article Title: Efficient genome editing of wild strawberry genes, vector development and validation
    Article Snippet: .. Two guide RNA sequences were inserted into entry vector JH4 by BsaI digestion/ligation and Q5 site‐directed mutagenesis kit (NEB), respectively, through two primer pairs (Fv ARF8‐F/Fv ARF8‐R and Fv ARF8‐F3/Fv ARF8‐R3) (Table ). ..

    Article Title: The stability of CREB3/Luman is regulated by protein kinase CK2 phosphorylation.
    Article Snippet: .. CREB3 (Luman) is a family member of ER resident transcription factors, which are cleaved upon the induction of ER stress. .. CREB3 (Luman) is a family member of ER resident transcription factors, which are cleaved upon the induction of ER stress.

    Article Title: Mutant p53 protects ETS2 from non-canonical COP1/DET1 dependent degradation
    Article Snippet: .. All mutagenesis was accomplished using the Q5® Site-Directed Mutagenesis Kit from New England BioLabs (Ipswich, MA, USA) and appropriate mutagenesis primers generated by using the NEBaseChanger™ tool. .. Determination of protein stability A549 cells were transfected with siRNA targeting either Control, Cdhl, COP1, Cul4a, DET1, and Skpla using Lipofectamine® RNAiMAX according to the manufacturer's protocol (Life Technologies, Grand Island, NY, USA).

    Article Title: The voltage-gated sodium channel EF-hands form an interaction with the III-IV linker that is disturbed by disease-causing mutations
    Article Snippet: .. Mutations in full-length constructs were introduced utilizing either QuikChange (Stratagene) or Q5® Site-Directed Mutagenesis Kit (New England BioLabs). .. Protein expression and purification Nav 1.5 constructs were expressed as His-MBP-tagged fusions with a TEV protease cleavage site from a modified pET-28 vector in E . coli Rosetta cells.

    Article Title: Truncated Variants of Gaussia Luciferase with Tyrosine Linker for Site-Specific Bioconjugate Applications
    Article Snippet: .. Once the truncated form was confirmed, a C-terminal tyrosine at the end of a linker peptide sequence was inserted into both the full-length and truncated variants of Gluc using the Q5® Site-Directed Mutagenesis Kit (see for primer sequences). .. For the truncated Gluc, the PCR reaction used to insert the C-terminal tyrosine linker simultaneously deleted the second homologous domain from pGluc (see truncation site marked with (^) in ) to eliminate the potential for stop codon read-through.

    Article Title: Structure and mechanism of a bacterial t6A biosynthesis system
    Article Snippet: .. Single-site mutagenesis of T. maritima TsaE Point mutagenesis was carried out on TsaE (cloned in pET-28a) using the Q5® Site-Directed Mutagenesis Kit (NEW ENGLAND Biolabs Inc.). ..

    Article Title: Efficient genome editing of wild strawberry genes, vector development and validation
    Article Snippet: .. For this dual sgRNA entry vector, one seed RNA is inserted at the BsaI site driven by the FveU6‐2 promoter, and a second seed RNA is inserted into the JH4 vector via Q5 site‐directed mutagenesis kit (NEB, Ipswich, MA) and driven by the AtU6‐26 promoter. ..

    Construct:

    Article Title: The voltage-gated sodium channel EF-hands form an interaction with the III-IV linker that is disturbed by disease-causing mutations
    Article Snippet: .. Mutations in full-length constructs were introduced utilizing either QuikChange (Stratagene) or Q5® Site-Directed Mutagenesis Kit (New England BioLabs). .. Protein expression and purification Nav 1.5 constructs were expressed as His-MBP-tagged fusions with a TEV protease cleavage site from a modified pET-28 vector in E . coli Rosetta cells.

    Generated:

    Article Title: Mutant p53 protects ETS2 from non-canonical COP1/DET1 dependent degradation
    Article Snippet: .. All mutagenesis was accomplished using the Q5® Site-Directed Mutagenesis Kit from New England BioLabs (Ipswich, MA, USA) and appropriate mutagenesis primers generated by using the NEBaseChanger™ tool. .. Determination of protein stability A549 cells were transfected with siRNA targeting either Control, Cdhl, COP1, Cul4a, DET1, and Skpla using Lipofectamine® RNAiMAX according to the manufacturer's protocol (Life Technologies, Grand Island, NY, USA).

    Sequencing:

    Article Title: Truncated Variants of Gaussia Luciferase with Tyrosine Linker for Site-Specific Bioconjugate Applications
    Article Snippet: .. Once the truncated form was confirmed, a C-terminal tyrosine at the end of a linker peptide sequence was inserted into both the full-length and truncated variants of Gluc using the Q5® Site-Directed Mutagenesis Kit (see for primer sequences). .. For the truncated Gluc, the PCR reaction used to insert the C-terminal tyrosine linker simultaneously deleted the second homologous domain from pGluc (see truncation site marked with (^) in ) to eliminate the potential for stop codon read-through.

    Plasmid Preparation:

    Article Title: Efficient genome editing of wild strawberry genes, vector development and validation
    Article Snippet: .. Two guide RNA sequences were inserted into entry vector JH4 by BsaI digestion/ligation and Q5 site‐directed mutagenesis kit (NEB), respectively, through two primer pairs (Fv ARF8‐F/Fv ARF8‐R and Fv ARF8‐F3/Fv ARF8‐R3) (Table ). ..

    Article Title: The stability of CREB3/Luman is regulated by protein kinase CK2 phosphorylation.
    Article Snippet: .. CREB3 (Luman) is a family member of ER resident transcription factors, which are cleaved upon the induction of ER stress. .. CREB3 (Luman) is a family member of ER resident transcription factors, which are cleaved upon the induction of ER stress.

    Article Title: Efficient genome editing of wild strawberry genes, vector development and validation
    Article Snippet: .. For this dual sgRNA entry vector, one seed RNA is inserted at the BsaI site driven by the FveU6‐2 promoter, and a second seed RNA is inserted into the JH4 vector via Q5 site‐directed mutagenesis kit (NEB, Ipswich, MA) and driven by the AtU6‐26 promoter. ..

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  • 99
    New England Biolabs pcr based mutagenesis strategy
    Pcr Based Mutagenesis Strategy, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr based mutagenesis strategy/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr based mutagenesis strategy - by Bioz Stars, 2020-07
    99/100 stars
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