pcr 2 1 topo vectors  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89

    Structured Review

    Thermo Fisher pcr 2 1 topo vectors
    Pcr 2 1 Topo Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vectors/product/Thermo Fisher
    Average 89 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo vectors - by Bioz Stars, 2020-08
    89/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon
    Article Snippet: .. PCR products were purified by QIAquick gel extraction kit (Qiagen, Valencia, CA) and cloned into pCR-2.1-TOPO® vectors (Invitrogen, Carlsbad, CA) using the TOPO-TA Cloning Kit for sequencing according to the manufacturer’s instructions. ..

    Article Title: Sphingosine Kinase 1 Is Regulated by Peroxisome Proliferator-activated Receptor α in Response to Free Fatty Acids and Is Essential for Skeletal Muscle Interleukin-6 Production and Signaling in Diet-induced Obesity *
    Article Snippet: .. PCR products were cloned into pCR-2.1 TOPO vectors (Invitrogen) and subcloned into PGL3-basic vectors (Promega). .. The full-length Sphk1 promoter or the deletion constructs were transfected into C2C12 myoblasts using Lipofectamine 2000 (Invitrogen), according to the manufacturer's protocol.

    Article Title: Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Article Snippet: .. Bisulfite-treated DNA was amplified by PCR and cloned into the pCR 2.1-TOPO vectors (Invitrogen); positive clones were examined by DNA sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (ABI), following the vendor’s instructions. .. Sequences extracted from individual clones were then analyzed to reveal the status of CpG methylation for the Oct3/4 and Nanog loci ( ).

    Article Title: Composition of protein supplements used for human embryo culture
    Article Snippet: .. Primer specificity was determined by melt curve analysis and PCR products were cloned into pCR 2.1 TOPO vectors and transformed into One Shot TOP10 chemically competent E. coli (Invitrogen Life Technologies; Grand Island, NY). .. Plasmids were sequenced by the DNA sequencing facility at Colorado State University (Fort Collins, CO) to confirm the identity of the transcript and plasmids were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies).

    Article Title: Preeclampsia Is Associated with Alterations in DNA Methylation of Genes Involved in Collagen Metabolism
    Article Snippet: .. PCR products were purified with a QIAquick PCR Purification kit (Qiagen), and 1 μL of each product was cloned into pCR 2.1-TOPO vectors using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA). .. After bacteria amplification, eight cloned PCR segments for each patient were sequenced in the Nucleic Acids Research Facilities at Virginia Commonwealth University.

    Article Title: Cloning and expression of two fragments of the S gene of canine coronavirus type I.
    Article Snippet: .. The amplified products were cloned into pCR ® 2.1-TOPO ® vectors (TOPO TA Cloning ® , Invitrogen, Milan, Italy). .. The nucleotide sequence of each insert was subjected to sequence analysis (Genome Express: Labo Grenoble, France) to confirm that no mutations had occurred during the amplification reaction.

    Article Title: Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment
    Article Snippet: .. Resulting PCR products were cloned into pCR 2.1 TOPO vectors and transformed into One Shot TOP10 chemically competent E. coli (Invitrogen). .. The plasmids were sequenced by the DNA sequencing facility at Colorado State University (Fort Collins, CO) to confirm the identity of the transcript.

    Amplification:

    Article Title: Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Article Snippet: .. Bisulfite-treated DNA was amplified by PCR and cloned into the pCR 2.1-TOPO vectors (Invitrogen); positive clones were examined by DNA sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (ABI), following the vendor’s instructions. .. Sequences extracted from individual clones were then analyzed to reveal the status of CpG methylation for the Oct3/4 and Nanog loci ( ).

    Article Title: Cloning and expression of two fragments of the S gene of canine coronavirus type I.
    Article Snippet: .. The amplified products were cloned into pCR ® 2.1-TOPO ® vectors (TOPO TA Cloning ® , Invitrogen, Milan, Italy). .. The nucleotide sequence of each insert was subjected to sequence analysis (Genome Express: Labo Grenoble, France) to confirm that no mutations had occurred during the amplification reaction.

    TA Cloning:

    Article Title: Preeclampsia Is Associated with Alterations in DNA Methylation of Genes Involved in Collagen Metabolism
    Article Snippet: .. PCR products were purified with a QIAquick PCR Purification kit (Qiagen), and 1 μL of each product was cloned into pCR 2.1-TOPO vectors using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA). .. After bacteria amplification, eight cloned PCR segments for each patient were sequenced in the Nucleic Acids Research Facilities at Virginia Commonwealth University.

    Article Title: Cloning and expression of two fragments of the S gene of canine coronavirus type I.
    Article Snippet: .. The amplified products were cloned into pCR ® 2.1-TOPO ® vectors (TOPO TA Cloning ® , Invitrogen, Milan, Italy). .. The nucleotide sequence of each insert was subjected to sequence analysis (Genome Express: Labo Grenoble, France) to confirm that no mutations had occurred during the amplification reaction.

    Purification:

    Article Title: Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon
    Article Snippet: .. PCR products were purified by QIAquick gel extraction kit (Qiagen, Valencia, CA) and cloned into pCR-2.1-TOPO® vectors (Invitrogen, Carlsbad, CA) using the TOPO-TA Cloning Kit for sequencing according to the manufacturer’s instructions. ..

    Article Title: Microbial Community Changes in Response to Ethanol or Methanol Amendments for U(VI) Reduction ▿
    Article Snippet: .. Following purification, PCR products were ligated in pCR 2.1-TOPO vectors (Invitrogen, Carlsbad, CA), transformed into One Shot Mach1-T1R (Invitrogen, Carlsbad, CA) chemically competent E. coli , and plated onto LB agar containing 50 μg ml−1 kanamycin and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside. .. White colonies (typically 90 to 100) resulting from growth of transformants incubated overnight at 37°C were selected.

    Article Title: Preeclampsia Is Associated with Alterations in DNA Methylation of Genes Involved in Collagen Metabolism
    Article Snippet: .. PCR products were purified with a QIAquick PCR Purification kit (Qiagen), and 1 μL of each product was cloned into pCR 2.1-TOPO vectors using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA). .. After bacteria amplification, eight cloned PCR segments for each patient were sequenced in the Nucleic Acids Research Facilities at Virginia Commonwealth University.

    Polymerase Chain Reaction:

    Article Title: Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon
    Article Snippet: .. PCR products were purified by QIAquick gel extraction kit (Qiagen, Valencia, CA) and cloned into pCR-2.1-TOPO® vectors (Invitrogen, Carlsbad, CA) using the TOPO-TA Cloning Kit for sequencing according to the manufacturer’s instructions. ..

    Article Title: Sphingosine Kinase 1 Is Regulated by Peroxisome Proliferator-activated Receptor α in Response to Free Fatty Acids and Is Essential for Skeletal Muscle Interleukin-6 Production and Signaling in Diet-induced Obesity *
    Article Snippet: .. PCR products were cloned into pCR-2.1 TOPO vectors (Invitrogen) and subcloned into PGL3-basic vectors (Promega). .. The full-length Sphk1 promoter or the deletion constructs were transfected into C2C12 myoblasts using Lipofectamine 2000 (Invitrogen), according to the manufacturer's protocol.

    Article Title: Microbial Community Changes in Response to Ethanol or Methanol Amendments for U(VI) Reduction ▿
    Article Snippet: .. Following purification, PCR products were ligated in pCR 2.1-TOPO vectors (Invitrogen, Carlsbad, CA), transformed into One Shot Mach1-T1R (Invitrogen, Carlsbad, CA) chemically competent E. coli , and plated onto LB agar containing 50 μg ml−1 kanamycin and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside. .. White colonies (typically 90 to 100) resulting from growth of transformants incubated overnight at 37°C were selected.

    Article Title: Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Article Snippet: .. Bisulfite-treated DNA was amplified by PCR and cloned into the pCR 2.1-TOPO vectors (Invitrogen); positive clones were examined by DNA sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (ABI), following the vendor’s instructions. .. Sequences extracted from individual clones were then analyzed to reveal the status of CpG methylation for the Oct3/4 and Nanog loci ( ).

    Article Title: Composition of protein supplements used for human embryo culture
    Article Snippet: .. Primer specificity was determined by melt curve analysis and PCR products were cloned into pCR 2.1 TOPO vectors and transformed into One Shot TOP10 chemically competent E. coli (Invitrogen Life Technologies; Grand Island, NY). .. Plasmids were sequenced by the DNA sequencing facility at Colorado State University (Fort Collins, CO) to confirm the identity of the transcript and plasmids were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies).

    Article Title: Preeclampsia Is Associated with Alterations in DNA Methylation of Genes Involved in Collagen Metabolism
    Article Snippet: .. PCR products were purified with a QIAquick PCR Purification kit (Qiagen), and 1 μL of each product was cloned into pCR 2.1-TOPO vectors using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA). .. After bacteria amplification, eight cloned PCR segments for each patient were sequenced in the Nucleic Acids Research Facilities at Virginia Commonwealth University.

    Article Title: Cloning and expression of two fragments of the S gene of canine coronavirus type I.
    Article Snippet: .. The amplified products were cloned into pCR ® 2.1-TOPO ® vectors (TOPO TA Cloning ® , Invitrogen, Milan, Italy). .. The nucleotide sequence of each insert was subjected to sequence analysis (Genome Express: Labo Grenoble, France) to confirm that no mutations had occurred during the amplification reaction.

    Article Title: Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment
    Article Snippet: .. Resulting PCR products were cloned into pCR 2.1 TOPO vectors and transformed into One Shot TOP10 chemically competent E. coli (Invitrogen). .. The plasmids were sequenced by the DNA sequencing facility at Colorado State University (Fort Collins, CO) to confirm the identity of the transcript.

    DNA Sequencing:

    Article Title: Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Article Snippet: .. Bisulfite-treated DNA was amplified by PCR and cloned into the pCR 2.1-TOPO vectors (Invitrogen); positive clones were examined by DNA sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (ABI), following the vendor’s instructions. .. Sequences extracted from individual clones were then analyzed to reveal the status of CpG methylation for the Oct3/4 and Nanog loci ( ).

    Sequencing:

    Article Title: Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon
    Article Snippet: .. PCR products were purified by QIAquick gel extraction kit (Qiagen, Valencia, CA) and cloned into pCR-2.1-TOPO® vectors (Invitrogen, Carlsbad, CA) using the TOPO-TA Cloning Kit for sequencing according to the manufacturer’s instructions. ..

    Article Title: Murine induced pluripotent stem cells can be derived from and differentiate into natural killer T cells
    Article Snippet: .. Bisulfite-treated DNA was amplified by PCR and cloned into the pCR 2.1-TOPO vectors (Invitrogen); positive clones were examined by DNA sequencing using the BigDye Terminator v3.1 Cycle Sequencing Kit (ABI), following the vendor’s instructions. .. Sequences extracted from individual clones were then analyzed to reveal the status of CpG methylation for the Oct3/4 and Nanog loci ( ).

    Gel Extraction:

    Article Title: Laser capture microdissection and metagenomic analysis of intact mucosa-associated microbial communities of human colon
    Article Snippet: .. PCR products were purified by QIAquick gel extraction kit (Qiagen, Valencia, CA) and cloned into pCR-2.1-TOPO® vectors (Invitrogen, Carlsbad, CA) using the TOPO-TA Cloning Kit for sequencing according to the manufacturer’s instructions. ..

    Transformation Assay:

    Article Title: Microbial Community Changes in Response to Ethanol or Methanol Amendments for U(VI) Reduction ▿
    Article Snippet: .. Following purification, PCR products were ligated in pCR 2.1-TOPO vectors (Invitrogen, Carlsbad, CA), transformed into One Shot Mach1-T1R (Invitrogen, Carlsbad, CA) chemically competent E. coli , and plated onto LB agar containing 50 μg ml−1 kanamycin and 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside. .. White colonies (typically 90 to 100) resulting from growth of transformants incubated overnight at 37°C were selected.

    Article Title: Composition of protein supplements used for human embryo culture
    Article Snippet: .. Primer specificity was determined by melt curve analysis and PCR products were cloned into pCR 2.1 TOPO vectors and transformed into One Shot TOP10 chemically competent E. coli (Invitrogen Life Technologies; Grand Island, NY). .. Plasmids were sequenced by the DNA sequencing facility at Colorado State University (Fort Collins, CO) to confirm the identity of the transcript and plasmids were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen Life Technologies).

    Article Title: Building a better mouse embryo assay: effects of mouse strain and in vitro maturation on sensitivity to contaminants of the culture environment
    Article Snippet: .. Resulting PCR products were cloned into pCR 2.1 TOPO vectors and transformed into One Shot TOP10 chemically competent E. coli (Invitrogen). .. The plasmids were sequenced by the DNA sequencing facility at Colorado State University (Fort Collins, CO) to confirm the identity of the transcript.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher pcr 2 1 topo ta cloning kit
    Pcr 2 1 Topo Ta Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo ta cloning kit/product/Thermo Fisher
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo ta cloning kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher topo 2 1 vector
    Topo 2 1 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/topo 2 1 vector/product/Thermo Fisher
    Average 91 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    topo 2 1 vector - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    91
    Thermo Fisher standard cloning vector
    Standard Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard cloning vector/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    standard cloning vector - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    94
    Thermo Fisher pcr 2 1 topo vector
    Pcr 2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Thermo Fisher
    Average 94 stars, based on 698 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo vector - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results