pcr 2 1 topo vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr 2 1 topo vector
    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR <t>2.1-TOPO</t> vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Pcr 2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Thermo Fisher
    Average 94 stars, based on 698 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo vector - by Bioz Stars, 2020-08
    94/100 stars

    Images

    1) Product Images from "The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype"

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype

    Journal: Molecular Pathology

    doi:

    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR 2.1-TOPO vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Figure Legend Snippet: (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR 2.1-TOPO vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.

    Techniques Used: Clone Assay, Polymerase Chain Reaction, Plasmid Preparation

    2) Product Images from "MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coli *"

    Article Title: MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coli *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.032904

    Primer extension analysis of MqsR cleavage sites in ompF mRNA in vivo . Total RNA was prepared from E. coli BL21 cells harboring pBAD- mqsR at the indicated time points before and after the induction of MqsR. The sequence ladders were obtained with pCR®2.1-Topo®-
    Figure Legend Snippet: Primer extension analysis of MqsR cleavage sites in ompF mRNA in vivo . Total RNA was prepared from E. coli BL21 cells harboring pBAD- mqsR at the indicated time points before and after the induction of MqsR. The sequence ladders were obtained with pCR®2.1-Topo®-

    Techniques Used: In Vivo, Sequencing, Polymerase Chain Reaction

    3) Product Images from "The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype"

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype

    Journal: Molecular Pathology

    doi:

    (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR 2.1-TOPO vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.
    Figure Legend Snippet: (A) Restriction digestion with EcoRI of clones containing monoclonal antibody 2G10 Vκ and VH chains (B) inserted into the pCR 2.1-TOPO vector. M1, 100 bp DNA ladder; M2, λDNA digested with HindIII. amp, ampicillin; L, leader; ori, origin; P, promoter.

    Techniques Used: Clone Assay, Polymerase Chain Reaction, Plasmid Preparation

    Related Articles

    Clone Assay:

    Article Title: Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2 3Diet, microbiota, and microbial metabolites in colon cancer risk
    Article Snippet: .. PCR products were purified by using the MinElute PCR purification Kit (Qiagen) and cloned into pCR 2.1 TOPO vector with a TOPO-TA cloning kit (Invitrogen). .. Plasmid DNA was isolated with a QIAprep Spin Miniprep kit (Qiagen), and plasmid DNA concentrations were measured spectrophotometrically (NanoDrop 1000; Thermol Scientific).

    Article Title: A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
    Article Snippet: .. CLONING AND SEQUENCING The PCR products obtained using poly(A) tailing and RT-PCR were cloned into the pCR ® 2.1-TOPO ® vector and transformed into competent E. coli DH5α (Life Technologies Japan, Tokyo, Japan). .. For RT-PCR, the cloned inserts were sequenced using an ABI 3130xl genetic analyzer (Life Technologies Japan) with the primers M13M4, M13rev, 926R/F , and 1390R ( ).

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen).

    Article Title: Human aging and somatic point mutations in mtDNA: A comparative study of generational differences (grandparents and grandchildren)
    Article Snippet: .. DNA from 12 grandmothers (three homoplasmic samples and nine randomly chosen heteroplasmic samples) was cloned using a pCR® 2.1-TOPO® vector from the TOPO TA cloning® kit (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Rice HYDROPEROXIDE LYASES with Unique Expression Patterns Generate Distinct Aldehyde Signatures in Arabidopsis 1
    Article Snippet: .. The band corresponding to each full-length gene was cut, purified using QIAquick Gel extraction kit (Qiagen), and cloned in pCR 2.1-TOPO Vector (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coli *
    Article Snippet: .. The promoter region of mqsR-ygiT was amplified by PCR with primers RT-proF and RT-proR and cloned into the pCR®2.1-Topo® vector (Invitrogen). ..

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Characterization of a non-sexual population of Strongyloides stercoralis with hybrid 18S rDNA haplotypes in Guangxi, Southern China
    Article Snippet: .. 3’ A-overhangs were added and cloned into pCR 2.1-TOPO vector and transfected into TOP10 competent E . coli cells by using the TOPO TA Cloning Kit (45–0641, Invitrogen) following the manufacturers protocol. .. For each S . stercoralis , multiple colonies were selected and cultured overnight at 37°C and 200 rpm in 2 ml LB medium containing 50 μg/ml ampicillin.

    Transfection:

    Article Title: Characterization of a non-sexual population of Strongyloides stercoralis with hybrid 18S rDNA haplotypes in Guangxi, Southern China
    Article Snippet: .. 3’ A-overhangs were added and cloned into pCR 2.1-TOPO vector and transfected into TOP10 competent E . coli cells by using the TOPO TA Cloning Kit (45–0641, Invitrogen) following the manufacturers protocol. .. For each S . stercoralis , multiple colonies were selected and cultured overnight at 37°C and 200 rpm in 2 ml LB medium containing 50 μg/ml ampicillin.

    Amplification:

    Article Title: MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coli *
    Article Snippet: .. The promoter region of mqsR-ygiT was amplified by PCR with primers RT-proF and RT-proR and cloned into the pCR®2.1-Topo® vector (Invitrogen). ..

    Ligation:

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    TA Cloning:

    Article Title: Human aging and somatic point mutations in mtDNA: A comparative study of generational differences (grandparents and grandchildren)
    Article Snippet: .. DNA from 12 grandmothers (three homoplasmic samples and nine randomly chosen heteroplasmic samples) was cloned using a pCR® 2.1-TOPO® vector from the TOPO TA cloning® kit (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Characterization of a non-sexual population of Strongyloides stercoralis with hybrid 18S rDNA haplotypes in Guangxi, Southern China
    Article Snippet: .. 3’ A-overhangs were added and cloned into pCR 2.1-TOPO vector and transfected into TOP10 competent E . coli cells by using the TOPO TA Cloning Kit (45–0641, Invitrogen) following the manufacturers protocol. .. For each S . stercoralis , multiple colonies were selected and cultured overnight at 37°C and 200 rpm in 2 ml LB medium containing 50 μg/ml ampicillin.

    Purification:

    Article Title: Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2 3Diet, microbiota, and microbial metabolites in colon cancer risk
    Article Snippet: .. PCR products were purified by using the MinElute PCR purification Kit (Qiagen) and cloned into pCR 2.1 TOPO vector with a TOPO-TA cloning kit (Invitrogen). .. Plasmid DNA was isolated with a QIAprep Spin Miniprep kit (Qiagen), and plasmid DNA concentrations were measured spectrophotometrically (NanoDrop 1000; Thermol Scientific).

    Article Title: Rice HYDROPEROXIDE LYASES with Unique Expression Patterns Generate Distinct Aldehyde Signatures in Arabidopsis 1
    Article Snippet: .. The band corresponding to each full-length gene was cut, purified using QIAquick Gel extraction kit (Qiagen), and cloned in pCR 2.1-TOPO Vector (Invitrogen) according to the manufacturer's instructions. ..

    Sequencing:

    Article Title: A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
    Article Snippet: .. CLONING AND SEQUENCING The PCR products obtained using poly(A) tailing and RT-PCR were cloned into the pCR ® 2.1-TOPO ® vector and transformed into competent E. coli DH5α (Life Technologies Japan, Tokyo, Japan). .. For RT-PCR, the cloned inserts were sequenced using an ABI 3130xl genetic analyzer (Life Technologies Japan) with the primers M13M4, M13rev, 926R/F , and 1390R ( ).

    Polymerase Chain Reaction:

    Article Title: Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2 3Diet, microbiota, and microbial metabolites in colon cancer risk
    Article Snippet: .. PCR products were purified by using the MinElute PCR purification Kit (Qiagen) and cloned into pCR 2.1 TOPO vector with a TOPO-TA cloning kit (Invitrogen). .. Plasmid DNA was isolated with a QIAprep Spin Miniprep kit (Qiagen), and plasmid DNA concentrations were measured spectrophotometrically (NanoDrop 1000; Thermol Scientific).

    Article Title: A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
    Article Snippet: .. CLONING AND SEQUENCING The PCR products obtained using poly(A) tailing and RT-PCR were cloned into the pCR ® 2.1-TOPO ® vector and transformed into competent E. coli DH5α (Life Technologies Japan, Tokyo, Japan). .. For RT-PCR, the cloned inserts were sequenced using an ABI 3130xl genetic analyzer (Life Technologies Japan) with the primers M13M4, M13rev, 926R/F , and 1390R ( ).

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen).

    Article Title: Human aging and somatic point mutations in mtDNA: A comparative study of generational differences (grandparents and grandchildren)
    Article Snippet: .. DNA from 12 grandmothers (three homoplasmic samples and nine randomly chosen heteroplasmic samples) was cloned using a pCR® 2.1-TOPO® vector from the TOPO TA cloning® kit (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Rice HYDROPEROXIDE LYASES with Unique Expression Patterns Generate Distinct Aldehyde Signatures in Arabidopsis 1
    Article Snippet: .. The band corresponding to each full-length gene was cut, purified using QIAquick Gel extraction kit (Qiagen), and cloned in pCR 2.1-TOPO Vector (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coli *
    Article Snippet: .. The promoter region of mqsR-ygiT was amplified by PCR with primers RT-proF and RT-proR and cloned into the pCR®2.1-Topo® vector (Invitrogen). ..

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Characterization of a non-sexual population of Strongyloides stercoralis with hybrid 18S rDNA haplotypes in Guangxi, Southern China
    Article Snippet: .. 3’ A-overhangs were added and cloned into pCR 2.1-TOPO vector and transfected into TOP10 competent E . coli cells by using the TOPO TA Cloning Kit (45–0641, Invitrogen) following the manufacturers protocol. .. For each S . stercoralis , multiple colonies were selected and cultured overnight at 37°C and 200 rpm in 2 ml LB medium containing 50 μg/ml ampicillin.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
    Article Snippet: .. CLONING AND SEQUENCING The PCR products obtained using poly(A) tailing and RT-PCR were cloned into the pCR ® 2.1-TOPO ® vector and transformed into competent E. coli DH5α (Life Technologies Japan, Tokyo, Japan). .. For RT-PCR, the cloned inserts were sequenced using an ABI 3130xl genetic analyzer (Life Technologies Japan) with the primers M13M4, M13rev, 926R/F , and 1390R ( ).

    Gel Extraction:

    Article Title: Rice HYDROPEROXIDE LYASES with Unique Expression Patterns Generate Distinct Aldehyde Signatures in Arabidopsis 1
    Article Snippet: .. The band corresponding to each full-length gene was cut, purified using QIAquick Gel extraction kit (Qiagen), and cloned in pCR 2.1-TOPO Vector (Invitrogen) according to the manufacturer's instructions. ..

    Transformation Assay:

    Article Title: A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
    Article Snippet: .. CLONING AND SEQUENCING The PCR products obtained using poly(A) tailing and RT-PCR were cloned into the pCR ® 2.1-TOPO ® vector and transformed into competent E. coli DH5α (Life Technologies Japan, Tokyo, Japan). .. For RT-PCR, the cloned inserts were sequenced using an ABI 3130xl genetic analyzer (Life Technologies Japan) with the primers M13M4, M13rev, 926R/F , and 1390R ( ).

    Plasmid Preparation:

    Article Title: Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans 1 2 3Diet, microbiota, and microbial metabolites in colon cancer risk
    Article Snippet: .. PCR products were purified by using the MinElute PCR purification Kit (Qiagen) and cloned into pCR 2.1 TOPO vector with a TOPO-TA cloning kit (Invitrogen). .. Plasmid DNA was isolated with a QIAprep Spin Miniprep kit (Qiagen), and plasmid DNA concentrations were measured spectrophotometrically (NanoDrop 1000; Thermol Scientific).

    Article Title: A comparative study of microbial diversity and community structure in marine sediments using poly(A) tailing and reverse transcription-PCR
    Article Snippet: .. CLONING AND SEQUENCING The PCR products obtained using poly(A) tailing and RT-PCR were cloned into the pCR ® 2.1-TOPO ® vector and transformed into competent E. coli DH5α (Life Technologies Japan, Tokyo, Japan). .. For RT-PCR, the cloned inserts were sequenced using an ABI 3130xl genetic analyzer (Life Technologies Japan) with the primers M13M4, M13rev, 926R/F , and 1390R ( ).

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Compared with that of mAb 2C7, this procedure involved an extra cloning step with the pCR 2.1-TOPO vector (Invitrogen). .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen).

    Article Title: Human aging and somatic point mutations in mtDNA: A comparative study of generational differences (grandparents and grandchildren)
    Article Snippet: .. DNA from 12 grandmothers (three homoplasmic samples and nine randomly chosen heteroplasmic samples) was cloned using a pCR® 2.1-TOPO® vector from the TOPO TA cloning® kit (Life Technologies, Carlsbad, CA, USA). ..

    Article Title: Rice HYDROPEROXIDE LYASES with Unique Expression Patterns Generate Distinct Aldehyde Signatures in Arabidopsis 1
    Article Snippet: .. The band corresponding to each full-length gene was cut, purified using QIAquick Gel extraction kit (Qiagen), and cloned in pCR 2.1-TOPO Vector (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: MqsR, a Crucial Regulator for Quorum Sensing and Biofilm Formation, Is a GCU-specific mRNA Interferase in Escherichia coli *
    Article Snippet: .. The promoter region of mqsR-ygiT was amplified by PCR with primers RT-proF and RT-proR and cloned into the pCR®2.1-Topo® vector (Invitrogen). ..

    Article Title: The production and characterisation of a chimaeric human IgE antibody, recognising the major mite allergen Der p 1, and its chimaeric human IgG1 anti-idiotype
    Article Snippet: .. Ligation of the PCR products of the HindIII–BamHI fragments, containing mAb 2G10 Vκ and VH, was performed within the EcoRI site of the pCR 2.1-TOPO vector, using the TOPO cloning kit (Invitrogen). .. Plasmids were purified using the QIAprep spin miniprep kit (Qiagen Limited) and bands containing mAb 2G10 Vκ and VH chains of the correct size were extracted using a QIAquick gel extraction kit (Qiagen Limited).

    Article Title: Characterization of a non-sexual population of Strongyloides stercoralis with hybrid 18S rDNA haplotypes in Guangxi, Southern China
    Article Snippet: .. 3’ A-overhangs were added and cloned into pCR 2.1-TOPO vector and transfected into TOP10 competent E . coli cells by using the TOPO TA Cloning Kit (45–0641, Invitrogen) following the manufacturers protocol. .. For each S . stercoralis , multiple colonies were selected and cultured overnight at 37°C and 200 rpm in 2 ml LB medium containing 50 μg/ml ampicillin.

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    Thermo Fisher pcr 2 1 topo vector
    Pcr 2 1 Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Thermo Fisher
    Average 94 stars, based on 698 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo vector - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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