Structured Review

Fisher Scientific pcr 2 1 topo vector
Pcr 2 1 Topo Vector, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr 2 1 topo vector/product/Fisher Scientific
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pcr 2 1 topo vector - by Bioz Stars, 2020-08
92/100 stars

Images

Related Articles

Clone Assay:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. This cassette was then cloned into pCR™2.1-TOPO® vector to form pMXT01 ( ). pMXT01 was digested with NotI/SpeI, and the 1,961 bp fragment was purified by agarose gel electrophoresis to eliminate intact plasmid and used as a template for cassette amplification. .. Deletion of endogenous BGCs has been used as a general strategy to 1) increase the metabolic flux of precursor availability and productivity of heterologously expressed BGCs, and 2) simplify the identification and characterization of heterologous products by activity screening and metabolite profiling ( ; ).

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. The final disruption cassette was further cloned into pCR™2.1-TOPO® vector (Fisher Scientific, Hampton, NH, USA) to give pMXT01 and was verified by restriction analysis and sequencing. .. The disruption cassette containing the Φ C31 phage attachment site attB , acc(3)IV (ApraR ), and oriT RK2 were jointly amplified from the 1,961-bp NotI/SpeI fragment of pMXT01 with the primer pair of salA-C_KO-F/salA-C_KO-R ( ).

Amplification:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. This cassette was then cloned into pCR™2.1-TOPO® vector to form pMXT01 ( ). pMXT01 was digested with NotI/SpeI, and the 1,961 bp fragment was purified by agarose gel electrophoresis to eliminate intact plasmid and used as a template for cassette amplification. .. Deletion of endogenous BGCs has been used as a general strategy to 1) increase the metabolic flux of precursor availability and productivity of heterologously expressed BGCs, and 2) simplify the identification and characterization of heterologous products by activity screening and metabolite profiling ( ; ).

Agarose Gel Electrophoresis:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. This cassette was then cloned into pCR™2.1-TOPO® vector to form pMXT01 ( ). pMXT01 was digested with NotI/SpeI, and the 1,961 bp fragment was purified by agarose gel electrophoresis to eliminate intact plasmid and used as a template for cassette amplification. .. Deletion of endogenous BGCs has been used as a general strategy to 1) increase the metabolic flux of precursor availability and productivity of heterologously expressed BGCs, and 2) simplify the identification and characterization of heterologous products by activity screening and metabolite profiling ( ; ).

Purification:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. This cassette was then cloned into pCR™2.1-TOPO® vector to form pMXT01 ( ). pMXT01 was digested with NotI/SpeI, and the 1,961 bp fragment was purified by agarose gel electrophoresis to eliminate intact plasmid and used as a template for cassette amplification. .. Deletion of endogenous BGCs has been used as a general strategy to 1) increase the metabolic flux of precursor availability and productivity of heterologously expressed BGCs, and 2) simplify the identification and characterization of heterologous products by activity screening and metabolite profiling ( ; ).

Polymerase Chain Reaction:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. This cassette was then cloned into pCR™2.1-TOPO® vector to form pMXT01 ( ). pMXT01 was digested with NotI/SpeI, and the 1,961 bp fragment was purified by agarose gel electrophoresis to eliminate intact plasmid and used as a template for cassette amplification. .. Deletion of endogenous BGCs has been used as a general strategy to 1) increase the metabolic flux of precursor availability and productivity of heterologously expressed BGCs, and 2) simplify the identification and characterization of heterologous products by activity screening and metabolite profiling ( ; ).

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. The final disruption cassette was further cloned into pCR™2.1-TOPO® vector (Fisher Scientific, Hampton, NH, USA) to give pMXT01 and was verified by restriction analysis and sequencing. .. The disruption cassette containing the Φ C31 phage attachment site attB , acc(3)IV (ApraR ), and oriT RK2 were jointly amplified from the 1,961-bp NotI/SpeI fragment of pMXT01 with the primer pair of salA-C_KO-F/salA-C_KO-R ( ).

Sequencing:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. The final disruption cassette was further cloned into pCR™2.1-TOPO® vector (Fisher Scientific, Hampton, NH, USA) to give pMXT01 and was verified by restriction analysis and sequencing. .. The disruption cassette containing the Φ C31 phage attachment site attB , acc(3)IV (ApraR ), and oriT RK2 were jointly amplified from the 1,961-bp NotI/SpeI fragment of pMXT01 with the primer pair of salA-C_KO-F/salA-C_KO-R ( ).

Plasmid Preparation:

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. This cassette was then cloned into pCR™2.1-TOPO® vector to form pMXT01 ( ). pMXT01 was digested with NotI/SpeI, and the 1,961 bp fragment was purified by agarose gel electrophoresis to eliminate intact plasmid and used as a template for cassette amplification. .. Deletion of endogenous BGCs has been used as a general strategy to 1) increase the metabolic flux of precursor availability and productivity of heterologously expressed BGCs, and 2) simplify the identification and characterization of heterologous products by activity screening and metabolite profiling ( ; ).

Article Title: Engineering Salinispora tropica for heterologous expression of natural product biosynthetic gene clusters
Article Snippet: .. The final disruption cassette was further cloned into pCR™2.1-TOPO® vector (Fisher Scientific, Hampton, NH, USA) to give pMXT01 and was verified by restriction analysis and sequencing. .. The disruption cassette containing the Φ C31 phage attachment site attB , acc(3)IV (ApraR ), and oriT RK2 were jointly amplified from the 1,961-bp NotI/SpeI fragment of pMXT01 with the primer pair of salA-C_KO-F/salA-C_KO-R ( ).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Fisher Scientific pcr 2 1 topo vector
    Pcr 2 1 Topo Vector, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo vector/product/Fisher Scientific
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo vector - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results