pcr 2 1 topo plasmid vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr 2 1 topo plasmid vector
    Pcr 2 1 Topo Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo plasmid vector/product/Thermo Fisher
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo plasmid vector - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
    Article Snippet: .. Cloning vector: pCRII-TOPO TA cloning kit (Invitrogen, cat. no. K4600-01). .. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300).

    Article Title: Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila
    Article Snippet: .. Topo TA Cloning Kit (Invitrogen, Carlsbad, California, United States) for amplicons fewer than two kilobases and Topo XL PCR Cloning Kit (Invitrogen) for larger fragments were used. .. FlyBase query and Repeat Masker software (with the latest release of the RepBase database update; http://www.girinst.org ) were used to assess the similarity of insertions with known sequence.

    Article Title: Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders
    Article Snippet: .. Those bands that were too large to directly sequence were TOPO cloned using the TOPO-TA PCR Cloning Kit (Invitrogen) and completely sequenced by random insertion of transposons as for S. grossa TOPO clones above. .. Completed TOPO clones were compared to the P. tepidariorum genome by BLASTN.

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    Article Title: Anaerobic Metabolism of Catechol by the Denitrifying Bacterium Thauera aromatica--a Result of Promiscuous Enzymes and Regulators? ▿
    Article Snippet: .. The 3-hydroxybenzoate-CoA ligase gene was cloned into the vector pEX5-CT/TOPO with a TOPO cloning kit from Invitrogen (Karlsruhe, Germany). .. The ligation mixture (1 μl of vector [10 ng of DNA], 1 μl of 6× ligation buffer, 4 μl of purified PCR product [50 ng of DNA]) was incubated at room temperature for 20 min. One microliter of the TOPO ligation reaction mixture was added to 200 μl of competent cells ( ) that were kept on ice for 10 min.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Article Title: Epigenetic and Genetic Alterations in Netrin-1 Receptors UNC5C and DCC in Human Colon Cancer
    Article Snippet: .. Each of the PCR products subsequently was cloned using the TOPO-TA cloning system (Invitrogen Life Technologies Inc). .. Nine to 11 white colonies indicating positive clones with PCR products were sequenced using ABI PRISM Big Dye Terminator v1.1 Cycle Sequencing Kits on an ABI PRISM 3100 Avant Genetic analyzer (Applied Biosystems, Foster City, CA).

    Amplification:

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    TA Cloning:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
    Article Snippet: .. Cloning vector: pCRII-TOPO TA cloning kit (Invitrogen, cat. no. K4600-01). .. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300).

    Article Title: Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila
    Article Snippet: .. Topo TA Cloning Kit (Invitrogen, Carlsbad, California, United States) for amplicons fewer than two kilobases and Topo XL PCR Cloning Kit (Invitrogen) for larger fragments were used. .. FlyBase query and Repeat Masker software (with the latest release of the RepBase database update; http://www.girinst.org ) were used to assess the similarity of insertions with known sequence.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Purification:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Sequencing:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders
    Article Snippet: .. Those bands that were too large to directly sequence were TOPO cloned using the TOPO-TA PCR Cloning Kit (Invitrogen) and completely sequenced by random insertion of transposons as for S. grossa TOPO clones above. .. Completed TOPO clones were compared to the P. tepidariorum genome by BLASTN.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Polymerase Chain Reaction:

    Article Title: Analysis of a novel AVPR2 mutation in a family with nephrogenic diabetes insipidus
    Article Snippet: .. For allele-specific sequence analysis, the purified PCR products were subcloned with a pCR2.1-TOPO TA Cloning® Kit (Invitrogen, Carlsbad, CA) and the cloned inserts were analyzed by PCR and sequenced. .. To investigate the novel frameshift mutation (302delC), we first created an expression vector for the wild type gene, and then introduced the frameshift mutation into it.

    Article Title: Heat-Shock Promoters: Targets for Evolution by P Transposable Elements in Drosophila
    Article Snippet: .. Topo TA Cloning Kit (Invitrogen, Carlsbad, California, United States) for amplicons fewer than two kilobases and Topo XL PCR Cloning Kit (Invitrogen) for larger fragments were used. .. FlyBase query and Repeat Masker software (with the latest release of the RepBase database update; http://www.girinst.org ) were used to assess the similarity of insertions with known sequence.

    Article Title: Duplication and concerted evolution of MiSp-encoding genes underlie the material properties of minor ampullate silks of cobweb weaving spiders
    Article Snippet: .. Those bands that were too large to directly sequence were TOPO cloned using the TOPO-TA PCR Cloning Kit (Invitrogen) and completely sequenced by random insertion of transposons as for S. grossa TOPO clones above. .. Completed TOPO clones were compared to the P. tepidariorum genome by BLASTN.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

    Article Title: Epigenetic and Genetic Alterations in Netrin-1 Receptors UNC5C and DCC in Human Colon Cancer
    Article Snippet: .. Each of the PCR products subsequently was cloned using the TOPO-TA cloning system (Invitrogen Life Technologies Inc). .. Nine to 11 white colonies indicating positive clones with PCR products were sequenced using ABI PRISM Big Dye Terminator v1.1 Cycle Sequencing Kits on an ABI PRISM 3100 Avant Genetic analyzer (Applied Biosystems, Foster City, CA).

    Transformation Assay:

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    Plasmid Preparation:

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells
    Article Snippet: .. Cloning vector: pCRII-TOPO TA cloning kit (Invitrogen, cat. no. K4600-01). .. Transcription reagents: Ribomax Large Scale RNA Production Kits (Promega, SP6 kit, cat. no. P1280, T7 kit, cat. no. P1300).

    Article Title: Generation of TALEN-Mediated GRdim Knock-In Rats by Homologous Recombination
    Article Snippet: .. Amplified DNA was cloned into blunt vector using the TOPO cloning kit (Invitrogen, now Life Technologies) and transformed into HB101 bacteria. ..

    Article Title: Anaerobic Metabolism of Catechol by the Denitrifying Bacterium Thauera aromatica--a Result of Promiscuous Enzymes and Regulators? ▿
    Article Snippet: .. The 3-hydroxybenzoate-CoA ligase gene was cloned into the vector pEX5-CT/TOPO with a TOPO cloning kit from Invitrogen (Karlsruhe, Germany). .. The ligation mixture (1 μl of vector [10 ng of DNA], 1 μl of 6× ligation buffer, 4 μl of purified PCR product [50 ng of DNA]) was incubated at room temperature for 20 min. One microliter of the TOPO ligation reaction mixture was added to 200 μl of competent cells ( ) that were kept on ice for 10 min.

    Article Title: Heritable CRISPR/Cas9-Mediated Genome Editing in the Yellow Fever Mosquito, Aedes aegypti
    Article Snippet: .. PCR products were either sequenced directly or cloned into a plasmid vector using the pCR4-TOPO TA cloning kit (Invitrogen, Life Technologies) prior to sequencing. .. From each Escherichia coli transformation, at least 10 colonies were picked and prepared for sequencing at the University of Missouri DNA Core.

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  • 91
    Thermo Fisher topo 2 1 vector
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    Thermo Fisher pcr 2 1 topo vector
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    https://www.bioz.com/result/pcr 2 1 topo vector/product/Thermo Fisher
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    Thermo Fisher pcr 2 1 topo ta vectors
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    https://www.bioz.com/result/pcr 2 1 topo ta vectors/product/Thermo Fisher
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    Price from $9.99 to $1999.99
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