Structured Review

Promega pcmv lacz plasmid
Pcmv Lacz Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv lacz plasmid/product/Promega
Average 88 stars, based on 4 article reviews
Price from $9.99 to $1999.99
pcmv lacz plasmid - by Bioz Stars, 2020-09
88/100 stars

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Transfection:

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: In Vitro Functional Analyses of Infrequent Nucleotide Variants in the Lactase Enhancer Reveal Different Molecular Routes to Increased Lactase Promoter Activity and Lactase Persistence
Article Snippet: .. Cells were transfected with a total DNA amount of 0.3 μg per well including 0.05 μg luciferase reporter gene plasmid, 0.025 μg pCMV‐lacZ plasmid (Promega) and 0.225 μg pBluescript SK+ plasmid (Stratagene) in a 25 μl volume containing 15 mM NaCl. .. A total of 25 μl of transfection reagent containing 2 μM polyethyleneimine (PEI) in form of Exgen 500 (22 kDa, Fermentas) in 10 mM NaCl solution or PEI25 (25 kDa, Alfa Aesar) were added to the DNA mix.

Luciferase:

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: In Vitro Functional Analyses of Infrequent Nucleotide Variants in the Lactase Enhancer Reveal Different Molecular Routes to Increased Lactase Promoter Activity and Lactase Persistence
Article Snippet: .. Cells were transfected with a total DNA amount of 0.3 μg per well including 0.05 μg luciferase reporter gene plasmid, 0.025 μg pCMV‐lacZ plasmid (Promega) and 0.225 μg pBluescript SK+ plasmid (Stratagene) in a 25 μl volume containing 15 mM NaCl. .. A total of 25 μl of transfection reagent containing 2 μM polyethyleneimine (PEI) in form of Exgen 500 (22 kDa, Fermentas) in 10 mM NaCl solution or PEI25 (25 kDa, Alfa Aesar) were added to the DNA mix.

Activity Assay:

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Expressing:

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Sequencing:

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Plasmid Preparation:

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: Aristolochic acid I and ochratoxin A differentially regulate VEGF expression in porcine kidney epithelial cells--The involvement of SP-1 and HIFs transcription factors
Article Snippet: .. The pCMV-lacZ plasmid containing the β-galactosidase (β-gal) gene driven by CMV promoter was from Promega and was co-transfected to cells together with one of the above described reporter vectors. .. The activity of reporter gene, luciferase, β-gal or SEAP was determined in cell lysates or cell culture media, respectively.

Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
Article Snippet: .. Transcriptional activity of Nrf2 was performed on HAoECs cotransfected with 0.4 μ g of plasmid ARE-luc containing the sequence ARE driving the expression of luciferase and with 0.1 μ g of the pCMV-LacZ plasmid containing the β -galactosidase gene driven by the CMV promoter (Promega) using Lipofectamine 2000 Transfection Reagent (Life Technologies) as previously described [ ]. .. At 48 h after transfection, cells were stimulated with simvastatin (1 and 10 μ M) or sulforaphane (SFN; 10 μ M) for 6 h. Luciferase activity was quantified using the Luciferase Assay System (Promega), according to the manufacturer's protocols.

Article Title: In Vitro Functional Analyses of Infrequent Nucleotide Variants in the Lactase Enhancer Reveal Different Molecular Routes to Increased Lactase Promoter Activity and Lactase Persistence
Article Snippet: .. Cells were transfected with a total DNA amount of 0.3 μg per well including 0.05 μg luciferase reporter gene plasmid, 0.025 μg pCMV‐lacZ plasmid (Promega) and 0.225 μg pBluescript SK+ plasmid (Stratagene) in a 25 μl volume containing 15 mM NaCl. .. A total of 25 μl of transfection reagent containing 2 μM polyethyleneimine (PEI) in form of Exgen 500 (22 kDa, Fermentas) in 10 mM NaCl solution or PEI25 (25 kDa, Alfa Aesar) were added to the DNA mix.

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    Promega pcmv lacz plasmid
    Pcmv Lacz Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv lacz plasmid/product/Promega
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pcmv lacz plasmid - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    90
    Promega β galactosidase expression plasmid
    β Galactosidase Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β galactosidase expression plasmid/product/Promega
    Average 90 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    β galactosidase expression plasmid - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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