pcdna3 hisb  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcdna3 hisb
    Pcdna3 Hisb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna3 hisb/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcdna3 hisb - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: To express MS2 fusion proteins, a cDNA encoding an oligomerization-defective mutant of the MS2 coat protein (provided by J. Lykke-Andersen and J. Steitz) was amplified by PCR using a 5′ primer containing a sequence encoding the FLAG epitope and subcloned between HindIII and EcoRV sites of pcDNA3 to obtain pFLAG-MS2. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.

    Polymerase Chain Reaction:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: To express MS2 fusion proteins, a cDNA encoding an oligomerization-defective mutant of the MS2 coat protein (provided by J. Lykke-Andersen and J. Steitz) was amplified by PCR using a 5′ primer containing a sequence encoding the FLAG epitope and subcloned between HindIII and EcoRV sites of pcDNA3 to obtain pFLAG-MS2. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.

    Mutagenesis:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: To express MS2 fusion proteins, a cDNA encoding an oligomerization-defective mutant of the MS2 coat protein (provided by J. Lykke-Andersen and J. Steitz) was amplified by PCR using a 5′ primer containing a sequence encoding the FLAG epitope and subcloned between HindIII and EcoRV sites of pcDNA3 to obtain pFLAG-MS2. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.

    Subcloning:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively. .. Constructs expressing different regions of KSRP were made by PCR and inserted into pFLAG-MS2, pcDNA3-HisB, or pCMV-Tag 2B.

    Construct:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively. .. Constructs expressing different regions of KSRP were made by PCR and inserted into pFLAG-MS2, pcDNA3-HisB, or pCMV-Tag 2B.

    FLAG-tag:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: To express MS2 fusion proteins, a cDNA encoding an oligomerization-defective mutant of the MS2 coat protein (provided by J. Lykke-Andersen and J. Steitz) was amplified by PCR using a 5′ primer containing a sequence encoding the FLAG epitope and subcloned between HindIII and EcoRV sites of pcDNA3 to obtain pFLAG-MS2. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.

    Expressing:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: Constructs expressing human β-globin reporter mRNAs were described previously ( ) and were kindly provided by J. Lykke-Andersen and J. Steitz. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.

    Sequencing:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: To express MS2 fusion proteins, a cDNA encoding an oligomerization-defective mutant of the MS2 coat protein (provided by J. Lykke-Andersen and J. Steitz) was amplified by PCR using a 5′ primer containing a sequence encoding the FLAG epitope and subcloned between HindIII and EcoRV sites of pcDNA3 to obtain pFLAG-MS2. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.

    Plasmid Preparation:

    Article Title: Tethering KSRP, a Decay-Promoting AU-Rich Element-Binding Protein, to mRNAs Elicits mRNA Decay
    Article Snippet: To express reporter mRNAs containing AREs, a polylinker (5′-AGATCTATCGATCTGCAGGATATCGCGGCCGCGTCGACAAGCTTGCATGC-3′) was inserted into a BglII site immediately downstream of the stop codon of the rabbit β-globin gene, which was previously subcloned into a tetracycline (Tet)-regulatory vector, pTRE (BD Biosciences), and was kindly provided by G. Brewer. .. Xpress-tagged and FLAG-tagged KSRP were constructed by subcloning KSRP cDNA between EcoRI and XhoI sites of pcDNA3-HisB (Invitrogen) and pCMV-Tag 2B (Stratagene), respectively.