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GenScript pcdna3 1 c k dyk vector
Pcdna3 1 C K Dyk Vector, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 c k dyk vector/product/GenScript
Average 93 stars, based on 5 article reviews
Price from $9.99 to $1999.99
pcdna3 1 c k dyk vector - by Bioz Stars, 2020-09
93/100 stars

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Countercurrent Chromatography:

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Clone Assay:

Article Title: Drosophila Exo70 Is Essential for Neurite Extension and Survival under Thermal Stress
Article Snippet: .. Drosophila exo70 was cloned from an exo70 cDNA in pcDNA3.1 +/ C-(K)DYK vector purchased from Genscript (clone ID ). .. The forward primer used was 5′-GCG AAT TCA TGA ACA ATT TGG ACA GTT CAT TG-3′, with an EcoRI restriction site introduced to the 5′ end of the primer.

Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Selection:

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Stable Transfection:

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Synthesized:

Article Title: Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis
Article Snippet: .. A mouse Crls1 ( ) cDNA ORF clone in pcDNA3.1+/C-(K)DYK vector was synthesized by Genscript ( ). .. One million primary brown or beige-like adipocytes were electroporated on differentiation day 3 with sterile plasmid using a Nucleofector II device with Cell Line Nucleofector Kit L (Lonza VACA-1005) and protocol A-033.

Article Title: Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis
Article Snippet: .. Gain-of-Function in Primary Mouse Adipocytes A mouse Crls1 (NM_001024385) cDNA ORF clone in pcDNA3.1+/C-(K)DYK vector was synthesized by Genscript (OMu17939). .. One million primary brown or beige-like adipocytes were electroporated on differentiation day 3 with sterile plasmid using a Nucleofector II device with Cell Line Nucleofector Kit L (Lonza VACA-1005) and protocol A-033.

Mutagenesis:

Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

Construct:

Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Expressing:

Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Plasmid Preparation:

Article Title: Drosophila Exo70 Is Essential for Neurite Extension and Survival under Thermal Stress
Article Snippet: .. Drosophila exo70 was cloned from an exo70 cDNA in pcDNA3.1 +/ C-(K)DYK vector purchased from Genscript (clone ID ). .. The forward primer used was 5′-GCG AAT TCA TGA ACA ATT TGG ACA GTT CAT TG-3′, with an EcoRI restriction site introduced to the 5′ end of the primer.

Article Title: HIV-1 Nef Antagonizes SERINC5 Restriction by Downregulation of SERINC5 via the Endosome/Lysosome System
Article Snippet: .. The Ub K48R, K63R, and K48/63R mutants were created in the pCMV-HA-Ub vector by site-directed mutagenesis. pMSCV-CD4-FLAG expression vector was created by cloning the CD4 cDNA with a C-terminal FLAG into the pMSCVpuro vector after XhoI/EcoRI digestion. pCMV-CD4-FLAG was constructed by cloning CD4 into the pCMV6-Entry vector after BamHI/EcoRV digestion. pcDNA3.1-MHC-I-FLAG expressing the HLA-A gene ( ) from the pcDNA3.1+/C-(K)DYK vector was ordered from GenScript (product no. OHu21196D). pcDNA3.1-SF2Nef-V5 expression vector was created by directly cloning the Nef gene from HIV-1 SF2 strain into the pcDNA3.1D/V5-His-TOPO vector via the TOPO-cloning strategy (Invitrogen). .. SF2Nef G2A, ΔCAW, AxxA, RR/LL, LL/AA, ED/AA, and ΔFL mutants were created by site-directed mutagenesis in the pcDNA3.1-SF2Nef-V5 vector.

Article Title: Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo
Article Snippet: .. Rescue Expression Construct GenEZ GLO1 ORF clone (NM_006708.3; ATG GCA GAA CCG CAG CCC CCG TCC GGC GGC CTC ACG GAC GAG GCC GCC CTC AGT TGC TGC TCC GAC GCG GAC CCC AGT ACC AAG GAT TTT CTA TTG CAG CAG ACC ATG CTA CGA GTG AAG GAT CCT AAG AAG TCA CTG GAT TTT TAT ACT AGA GTT CTT GGA ATG ACG CTA ATC CAA AAA TGT GAT TTT CCC ATT ATG AAG TTT TCA CTC TAC TTC TTG GCT TAT GAG GAT AAA AAT GAC ATC CCT AAA GAA AAA GAT GAA AAA ATA GCC TGG GCG CTC TCC AGA AAA GCT ACA CTT GAG CTG ACA CAC AAT TGG GGC ACT GAA GAT GAT GAG ACC CAG AGT TAC CAC AAT GGC AAT TCA GAC CCT CGA GGA TTC GGT CAT ATT GGA ATT GCT GTT CCT GAT GTA TAC AGT GCT TGT AAA AGG TTT GAA GAA CTG GGA GTC AAA TTT GTG AAG AAA CCT GAT GAT GGTAAA ATG AAA GGC CTG GCA TTT ATT CAA GAT CCT GAT GGC TAC TGG ATT GAA ATT TTG AAT CCT AAC AAA ATG GCA ACC TTA ATG TAG) was cloned into the linearized pcDNA3.1 + /C-(K)DYK vector (5444 base pairs, containing Neomycin resistance gene for mammalian selection) using Seamless cloning technology (GenScript Biotech, Piscataway, NJ) used for stable transfection of human A375 melanoma cells. .. Briefly, stable A375-GLO1 rescue cells (A375-GLO1 _R) were generated by overnight incubation of A375-GLO1 _KO cells with DNA (4 µg)-Lipofectamine® 2000 (10 µg) complexes at 37 °C.

Article Title: Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis
Article Snippet: .. A mouse Crls1 ( ) cDNA ORF clone in pcDNA3.1+/C-(K)DYK vector was synthesized by Genscript ( ). .. One million primary brown or beige-like adipocytes were electroporated on differentiation day 3 with sterile plasmid using a Nucleofector II device with Cell Line Nucleofector Kit L (Lonza VACA-1005) and protocol A-033.

Article Title: Cardiolipin Synthesis in Brown and Beige Fat Mitochondria Is Essential for Systemic Energy Homeostasis
Article Snippet: .. Gain-of-Function in Primary Mouse Adipocytes A mouse Crls1 (NM_001024385) cDNA ORF clone in pcDNA3.1+/C-(K)DYK vector was synthesized by Genscript (OMu17939). .. One million primary brown or beige-like adipocytes were electroporated on differentiation day 3 with sterile plasmid using a Nucleofector II device with Cell Line Nucleofector Kit L (Lonza VACA-1005) and protocol A-033.

Article Title: CNGA3 acts as a cold sensor in hypothalamic neurons
Article Snippet: .. Plasmids Mouse Cnga2 (NCBI accession number: NM_007724.3) and Cngb1 (NCBI accession number: NM_001195413.1) in pcDNA3.1+/C-(K)DYK vector were obtained from a commercial source (CloneID: OMu19184 and OMu17438 respectively, GenScript, Piscataway, NJ) and subcloned into the pMO vector. .. Plasmids Mouse Cnga2 (NCBI accession number: NM_007724.3) and Cngb1 (NCBI accession number: NM_001195413.1) in pcDNA3.1+/C-(K)DYK vector were obtained from a commercial source (CloneID: OMu19184 and OMu17438 respectively, GenScript, Piscataway, NJ) and subcloned into the pMO vector.

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    GenScript pcdna3 1 c
    Pcdna3 1 C, supplied by GenScript, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna3 1 c/product/GenScript
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pcdna3 1 c - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    93
    GenScript pcdna3 1 c k dyk vector
    Pcdna3 1 C K Dyk Vector, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna3 1 c k dyk vector/product/GenScript
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pcdna3 1 c k dyk vector - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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