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Xenobiotics pcbs
Pcbs, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcbs/product/Xenobiotics
Average 92 stars, based on 1 article reviews
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pcbs - by Bioz Stars, 2020-09
92/100 stars

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Activity Assay:

Article Title: Seasonal Variations of the Activity of Antioxidant Defense Enzymes in the Red Mullet (Mullus barbatus l.) from the Adriatic Sea
Article Snippet: .. Induction of GR activity has been reported in various field studies in fish exposed to organic pollutants, such as PAHs, PCBs and halogenated xenobiotics [ , ]. .. Glutathione-S-transferases are a family of dimeric multifunctional enzymes that are shown to have been involved in detoxification of xenobiotics, protection from oxidative damage and the intracellular transport of hormones, endogenous metabolites and exogenous chemicals in diverse organisms [ ].

other:

Article Title: Metatranscriptome Analysis Deciphers Multifunctional Genes and Enzymes Linked With the Degradation of Aromatic Compounds and Pesticides in the Wheat Rhizosphere
Article Snippet: Polyaromatic, chlorinated and nitrobenzene compounds, dioxins, PCBs and the pesticides represent principal xenobiotics ( ; ) that cause biospheric pollution ( ).

Article Title: TOXICOKINETICS OF CHIRAL POLYCHLORINATED BIPHENYLS ACROSS DIFFERENT SPECIES—A REVIEW
Article Snippet: In mammals, such as rats, dogs and monkeys, the liver and muscle are the most important tissues for the initial distribution of PCBs because they are highly perfused, have a large mass and a high intrinsic ability to extract xenobiotics from blood ( , , ).

Fluorescence In Situ Hybridization:

Article Title: Seasonal Variations of the Activity of Antioxidant Defense Enzymes in the Red Mullet (Mullus barbatus l.) from the Adriatic Sea
Article Snippet: .. Induction of GR activity has been reported in various field studies in fish exposed to organic pollutants, such as PAHs, PCBs and halogenated xenobiotics [ , ]. .. Glutathione-S-transferases are a family of dimeric multifunctional enzymes that are shown to have been involved in detoxification of xenobiotics, protection from oxidative damage and the intracellular transport of hormones, endogenous metabolites and exogenous chemicals in diverse organisms [ ].

Purification:

Article Title: 2,2?,3,3?,6,6?-Hexachlorobiphenyl (PCB 136) Atropisomers Interact Enantioselectively with Hepatic Microsomal Cytochrome P450 Enzymes
Article Snippet: .. The interaction of endogenous compounds and xenobiotics such as PCBs with hepatic microsomal or purified P450 enzymes produces spectral changes due to a disturbance of the coordination sphere of the heme iron. ..

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  • 88
    Xenobiotics pcb 136
    Comparison of the levels of (A) 5-136, (B) 4-136, and (C) 4,5-136 in tissues and excreta from female mice with defective hepatic metabolism (KO mice) and their age-matched congenic wild type mice (WT). <t>PCB</t> 136 is primarily eliminated as 5-136, and to a lesser extent as (B) 4-136 and (C) 4,5-136, in the feces of KO and WT mice; however, the time course of fecal HO-PCB metabolite and/or conjugate levels differ significantly between KO and WT mice. Data represent the mean ± standard deviation of the HO-PCB metabolites levels determined in the tissues and excreta of individual, PCB l36-treated KO ( n = 7) and WT mice ( n = 5) and are expressed on a logarithmic scale as percent of the total PCB 136 dose (see Table S11 for additional details). * Significantly different from WT ( t test, p
    Pcb 136, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 136/product/Xenobiotics
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcb 136 - by Bioz Stars, 2020-09
    88/100 stars
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    92
    Xenobiotics pcb 126
    Principal component analysis of transcriptome profile alterations provoked by exposure of HepaRG cells to PCB 126. Transcript abundances were assessed using Stringtie. The PCA was performed using log2 transformed FPKM measurements of transcripts across samples assessed with Ballgown. The groups become more clustered as the dose of <t>PCB</t> 126 increases. The 95% confidence regions are displayed by ellipses
    Pcb 126, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 126/product/Xenobiotics
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pcb 126 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Xenobiotics parent pcb 118
    Incubations of <t>PCB</t> 118 in HLMs over time. Brackets indicate ±S.D.
    Parent Pcb 118, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/parent pcb 118/product/Xenobiotics
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    parent pcb 118 - by Bioz Stars, 2020-09
    85/100 stars
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    Image Search Results


    Comparison of the levels of (A) 5-136, (B) 4-136, and (C) 4,5-136 in tissues and excreta from female mice with defective hepatic metabolism (KO mice) and their age-matched congenic wild type mice (WT). PCB 136 is primarily eliminated as 5-136, and to a lesser extent as (B) 4-136 and (C) 4,5-136, in the feces of KO and WT mice; however, the time course of fecal HO-PCB metabolite and/or conjugate levels differ significantly between KO and WT mice. Data represent the mean ± standard deviation of the HO-PCB metabolites levels determined in the tissues and excreta of individual, PCB l36-treated KO ( n = 7) and WT mice ( n = 5) and are expressed on a logarithmic scale as percent of the total PCB 136 dose (see Table S11 for additional details). * Significantly different from WT ( t test, p

    Journal: Environmental Science & Technology

    Article Title: Hepatic Metabolism Affects the Atropselective Disposition of 2,2′,3,3′,6,6′-Hexachlorobiphenyl (PCB 136) in Mice

    doi: 10.1021/es504766p

    Figure Lengend Snippet: Comparison of the levels of (A) 5-136, (B) 4-136, and (C) 4,5-136 in tissues and excreta from female mice with defective hepatic metabolism (KO mice) and their age-matched congenic wild type mice (WT). PCB 136 is primarily eliminated as 5-136, and to a lesser extent as (B) 4-136 and (C) 4,5-136, in the feces of KO and WT mice; however, the time course of fecal HO-PCB metabolite and/or conjugate levels differ significantly between KO and WT mice. Data represent the mean ± standard deviation of the HO-PCB metabolites levels determined in the tissues and excreta of individual, PCB l36-treated KO ( n = 7) and WT mice ( n = 5) and are expressed on a logarithmic scale as percent of the total PCB 136 dose (see Table S11 for additional details). * Significantly different from WT ( t test, p

    Article Snippet: The large percentage of HO-PCBs recovered over the three day study period from feces of KO mice is surprising because, as we (see ) and others have shown, KO mice have essentially no hepatic CPR activity and, therefore, should not be able to metabolize PCB 136 in the liver; however, other xenobiotics, such as cyclophosphamide, also undergo considerable biotransformation in KO mice.

    Techniques: Mouse Assay, Standard Deviation

    Comparison of the enantiomeric fractions (EFs) of PCB 136 (A), 5-136 (B), and 4-136 (C) in tissues and feces reveals significant differences in the atropisomeric enrichment between female mice with a liver-specific deletion of the cpr gene (KO) and congenic wild type (WT) mice. The EF values are presented as the mean ± standard deviation and were determined in the tissues of individual PCB l36-treated KO ( n = 7) and WT mice ( n = 5). The only exceptions are the EF determination for 5-136 in blood, which were performed with a single sample pooled by genotype. A CB column was used to separated PCB 136 and 4-136, whereas a BDM column was used for separation of 5-136 (see Table S12 for additional details). * Significantly different from WT ( t test, p

    Journal: Environmental Science & Technology

    Article Title: Hepatic Metabolism Affects the Atropselective Disposition of 2,2′,3,3′,6,6′-Hexachlorobiphenyl (PCB 136) in Mice

    doi: 10.1021/es504766p

    Figure Lengend Snippet: Comparison of the enantiomeric fractions (EFs) of PCB 136 (A), 5-136 (B), and 4-136 (C) in tissues and feces reveals significant differences in the atropisomeric enrichment between female mice with a liver-specific deletion of the cpr gene (KO) and congenic wild type (WT) mice. The EF values are presented as the mean ± standard deviation and were determined in the tissues of individual PCB l36-treated KO ( n = 7) and WT mice ( n = 5). The only exceptions are the EF determination for 5-136 in blood, which were performed with a single sample pooled by genotype. A CB column was used to separated PCB 136 and 4-136, whereas a BDM column was used for separation of 5-136 (see Table S12 for additional details). * Significantly different from WT ( t test, p

    Article Snippet: The large percentage of HO-PCBs recovered over the three day study period from feces of KO mice is surprising because, as we (see ) and others have shown, KO mice have essentially no hepatic CPR activity and, therefore, should not be able to metabolize PCB 136 in the liver; however, other xenobiotics, such as cyclophosphamide, also undergo considerable biotransformation in KO mice.

    Techniques: Mouse Assay, Standard Deviation

    Simplified metabolism scheme of PCB 136 atropisomers showing major HO-PCB metabolites investigated and the corresponding abbreviations. Only one atropisomer of PCB 136 and the corresponding metabolites are shown for clarity reasons. P450, cytochrome P450 enzyme; UGT, uridine 5′-diphospho-glucuronosyltransferase; SULT, sulfotransferase; Glc, glucuronide.

    Journal: Environmental Science & Technology

    Article Title: Hepatic Metabolism Affects the Atropselective Disposition of 2,2′,3,3′,6,6′-Hexachlorobiphenyl (PCB 136) in Mice

    doi: 10.1021/es504766p

    Figure Lengend Snippet: Simplified metabolism scheme of PCB 136 atropisomers showing major HO-PCB metabolites investigated and the corresponding abbreviations. Only one atropisomer of PCB 136 and the corresponding metabolites are shown for clarity reasons. P450, cytochrome P450 enzyme; UGT, uridine 5′-diphospho-glucuronosyltransferase; SULT, sulfotransferase; Glc, glucuronide.

    Article Snippet: The large percentage of HO-PCBs recovered over the three day study period from feces of KO mice is surprising because, as we (see ) and others have shown, KO mice have essentially no hepatic CPR activity and, therefore, should not be able to metabolize PCB 136 in the liver; however, other xenobiotics, such as cyclophosphamide, also undergo considerable biotransformation in KO mice.

    Techniques: Gas Chromatography

    Female mice with defective hepatic metabolism due to liver-specific deletion of cytochrome P450 oxidoreductase (KO) have significantly higher levels of PCB 136 in tissues and feces compared to age-matched congenic wild type mice (WT). Data represent the mean ± standard deviation of the PCB 136 levels determined in the tissues and excreta of individual PCB l36-treated KO ( n = 7) and WT mice ( n = 5) and are expressed on a logarithmic scale as percent of the total PCB 136 dose (see Table S11 for additional details). * Significantly different from WT ( t test, p

    Journal: Environmental Science & Technology

    Article Title: Hepatic Metabolism Affects the Atropselective Disposition of 2,2′,3,3′,6,6′-Hexachlorobiphenyl (PCB 136) in Mice

    doi: 10.1021/es504766p

    Figure Lengend Snippet: Female mice with defective hepatic metabolism due to liver-specific deletion of cytochrome P450 oxidoreductase (KO) have significantly higher levels of PCB 136 in tissues and feces compared to age-matched congenic wild type mice (WT). Data represent the mean ± standard deviation of the PCB 136 levels determined in the tissues and excreta of individual PCB l36-treated KO ( n = 7) and WT mice ( n = 5) and are expressed on a logarithmic scale as percent of the total PCB 136 dose (see Table S11 for additional details). * Significantly different from WT ( t test, p

    Article Snippet: The large percentage of HO-PCBs recovered over the three day study period from feces of KO mice is surprising because, as we (see ) and others have shown, KO mice have essentially no hepatic CPR activity and, therefore, should not be able to metabolize PCB 136 in the liver; however, other xenobiotics, such as cyclophosphamide, also undergo considerable biotransformation in KO mice.

    Techniques: Mouse Assay, Standard Deviation

    Principal component analysis of transcriptome profile alterations provoked by exposure of HepaRG cells to PCB 126. Transcript abundances were assessed using Stringtie. The PCA was performed using log2 transformed FPKM measurements of transcripts across samples assessed with Ballgown. The groups become more clustered as the dose of PCB 126 increases. The 95% confidence regions are displayed by ellipses

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Principal component analysis of transcriptome profile alterations provoked by exposure of HepaRG cells to PCB 126. Transcript abundances were assessed using Stringtie. The PCA was performed using log2 transformed FPKM measurements of transcripts across samples assessed with Ballgown. The groups become more clustered as the dose of PCB 126 increases. The 95% confidence regions are displayed by ellipses

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques: Transformation Assay

    Sparse partial least squares regression (sPLS) integration of the metabolome and transcriptome profiles of HepaRG cells exposed to PCB 126 shows that alteration in sphingolipid levels is concomitant to a decrease in the activity of genes involved in lipid metabolism. a Individual plots displaying the covariance between the metabolome and the transcriptome datasets. b The variables selected by the sPLS are projected on a correlation circle plot to display the clusters of correlated variables. In this plot, the angle defined by the coordinates of the variables on the axis defined by the components give an indication on the nature of the correlation. If the angle is sharp and the variables cluster together, the correlation is positive. If the angle is obtuse and the variables are not clustered together, the variables are negatively correlated. Perpendicular angles represent uncorrelated variables. c A clustered image map visualizes correlations between the metabolites and the genes by a color gradient on a two-dimensional colored image. The negatively correlated variables (blue) are represented along the positively correlated variables (red). Dendrograms are added to represent the clusters produced by the hierarchical clustering

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Sparse partial least squares regression (sPLS) integration of the metabolome and transcriptome profiles of HepaRG cells exposed to PCB 126 shows that alteration in sphingolipid levels is concomitant to a decrease in the activity of genes involved in lipid metabolism. a Individual plots displaying the covariance between the metabolome and the transcriptome datasets. b The variables selected by the sPLS are projected on a correlation circle plot to display the clusters of correlated variables. In this plot, the angle defined by the coordinates of the variables on the axis defined by the components give an indication on the nature of the correlation. If the angle is sharp and the variables cluster together, the correlation is positive. If the angle is obtuse and the variables are not clustered together, the variables are negatively correlated. Perpendicular angles represent uncorrelated variables. c A clustered image map visualizes correlations between the metabolites and the genes by a color gradient on a two-dimensional colored image. The negatively correlated variables (blue) are represented along the positively correlated variables (red). Dendrograms are added to represent the clusters produced by the hierarchical clustering

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques: Activity Assay, Produced

    Differential  CYP1A1  expression analysis using RNA-seq in HepaRG cells exposed to three concentrations of PCB 126.  a  Structure and expression levels of 12 distinct isoforms of  CYP1A1  across the different treatment groups. Differences in expression levels are displayed in varying shades of yellow. The ENST00000395048 isoform of  CYP1A1  is expressed at a much higher level than the others, as indicated by the dark orange color.  b  FPKM distributions of four  CYP1A1  transcripts displayed as box-and-whiskers plots. All four isoforms have their expression significantly altered ( q

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Differential CYP1A1 expression analysis using RNA-seq in HepaRG cells exposed to three concentrations of PCB 126. a Structure and expression levels of 12 distinct isoforms of CYP1A1 across the different treatment groups. Differences in expression levels are displayed in varying shades of yellow. The ENST00000395048 isoform of CYP1A1 is expressed at a much higher level than the others, as indicated by the dark orange color. b FPKM distributions of four CYP1A1 transcripts displayed as box-and-whiskers plots. All four isoforms have their expression significantly altered ( q

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques: Expressing, RNA Sequencing Assay

    Pathway enrichment analysis in the transcriptome of HepaRG cells exposed to PCB 126 shows an activation of xenobiotic metabolism by dioxin-like compounds. Gene functions were studied using ClueGO and CluePedia plugins in Cytoscape (version 3.5.1). The analysis was conducted using a two-sided hypergeometric test for enrichment using a p value threshold of 0.05 after its adjustment by the Benjamini–Hochberg procedure

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Pathway enrichment analysis in the transcriptome of HepaRG cells exposed to PCB 126 shows an activation of xenobiotic metabolism by dioxin-like compounds. Gene functions were studied using ClueGO and CluePedia plugins in Cytoscape (version 3.5.1). The analysis was conducted using a two-sided hypergeometric test for enrichment using a p value threshold of 0.05 after its adjustment by the Benjamini–Hochberg procedure

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques: Activation Assay

    Multivariate analysis of HepaRG cell metabolome following treatment with PCB 126 shows alterations in lipid metabolism. a Principal component analysis of metabolome profiles separate the group of samples treated with the PCB126 from the control group. As the dose of PCB 126 increases, the groups become more clustered. b Orthogonal projection to latent structures discriminant analysis (OPLS-DA) properly classified all samples (R2X = 0.177, R2Y = 0.769, and Q2 = 0.58). The 95% confidence regions are displayed by shaded ellipses. c A 1000-time permutation test shows that the observed statistics is not part of the distribution formed by the statistics from the permuted data (R2Y p = 0.022; Q2 p

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Multivariate analysis of HepaRG cell metabolome following treatment with PCB 126 shows alterations in lipid metabolism. a Principal component analysis of metabolome profiles separate the group of samples treated with the PCB126 from the control group. As the dose of PCB 126 increases, the groups become more clustered. b Orthogonal projection to latent structures discriminant analysis (OPLS-DA) properly classified all samples (R2X = 0.177, R2Y = 0.769, and Q2 = 0.58). The 95% confidence regions are displayed by shaded ellipses. c A 1000-time permutation test shows that the observed statistics is not part of the distribution formed by the statistics from the permuted data (R2Y p = 0.022; Q2 p

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques:

    Network analysis of metabolome profile alterations demonstrates a role of taurine and hypotaurine in oxidative stress induced by PCB 126 in HepaRG cells. a Sub-network. Circles are metabolites and rectangles are reactions. Reaction labels are EC numbers and metabolite names are from Recon2 model. Red circle metabolites are the ones from the fingerprint. b Distance matrix between metabolites belonging to the network. Red corresponds to shorter distance (0) and white to longer distances (12 reactions between nodes)

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Network analysis of metabolome profile alterations demonstrates a role of taurine and hypotaurine in oxidative stress induced by PCB 126 in HepaRG cells. a Sub-network. Circles are metabolites and rectangles are reactions. Reaction labels are EC numbers and metabolite names are from Recon2 model. Red circle metabolites are the ones from the fingerprint. b Distance matrix between metabolites belonging to the network. Red corresponds to shorter distance (0) and white to longer distances (12 reactions between nodes)

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques:

    Box plot of metabolome changes associated with the exposure to PCB 126 to HepaRG cells shows a dose-dependent effect. All the metabolites displayed have their levels significantly altered ( q

    Journal: Archives of Toxicology

    Article Title: Integrated transcriptomics and metabolomics reveal signatures of lipid metabolism dysregulation in HepaRG liver cells exposed to PCB 126

    doi: 10.1007/s00204-018-2235-7

    Figure Lengend Snippet: Box plot of metabolome changes associated with the exposure to PCB 126 to HepaRG cells shows a dose-dependent effect. All the metabolites displayed have their levels significantly altered ( q

    Article Snippet: Unsurprisingly, the most important networks affected by PCB 126 were related to the metabolism of xenobiotics by P450 cytochromes (CYP1A1, CYP1A2, CYP1B1) and UDP glucuronosyltransferase (UGT1A1, UGT1A3, UGT2A3, UGT2B11), both being well-known PCB metabolic pathways.

    Techniques:

    CYP1A1 protein levels and enzyme activity are reduced in low oxygen environments Upper panel: HepG2 cells (A) and HaCaT cells (B) were subjected to normoxia or hypoxia for 16 hours prior to treatment with 3 µM PCB 126 for 48 hours or 24 hours, respecitively. Ten micrograms of whole cell lysate were western blotted for the presence of CYP1A1. Beta-tubulin was used as a loading control. Numbers over the lanes represent relative intensities of CYP1A1 compared to PCB 126 treated cells in normoxia. n.d. = not detectable, n.s. = non-specific band. Lower panel: HepG2 cells (C) and HaCaT cells (D) were incubated in normoxia or hypoxia for 16 hours before treatment with 3 µM PCB 126 for 24 hours. CYP1A1 activity was measured using a luminogenic CYP1A1 substrate and is depicted relative to untreated cells in normoxia. * = p

    Journal: Toxicology and applied pharmacology

    Article Title: Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines

    doi: 10.1016/j.taap.2013.12.002

    Figure Lengend Snippet: CYP1A1 protein levels and enzyme activity are reduced in low oxygen environments Upper panel: HepG2 cells (A) and HaCaT cells (B) were subjected to normoxia or hypoxia for 16 hours prior to treatment with 3 µM PCB 126 for 48 hours or 24 hours, respecitively. Ten micrograms of whole cell lysate were western blotted for the presence of CYP1A1. Beta-tubulin was used as a loading control. Numbers over the lanes represent relative intensities of CYP1A1 compared to PCB 126 treated cells in normoxia. n.d. = not detectable, n.s. = non-specific band. Lower panel: HepG2 cells (C) and HaCaT cells (D) were incubated in normoxia or hypoxia for 16 hours before treatment with 3 µM PCB 126 for 24 hours. CYP1A1 activity was measured using a luminogenic CYP1A1 substrate and is depicted relative to untreated cells in normoxia. * = p

    Article Snippet: As such, PCB 126 can bind to and activate the aryl hydrocarbon receptor (AhR), an immediate early cellular response to xenobiotics and mediator of many of the toxic effects of PCB exposure.

    Techniques: Activity Assay, Western Blot, Incubation

    Oxygen concentration dependent induction of CYP1A1 mRNA expression HepG2 cells (left panel: A-C) and HaCaT cells (right panel: D-F) were incubated in normoxia (21% O 2 ) or hypoxia (1% O 2 ) for 8 hours prior to treatment with vehicle (DMSO), 2–4 nM PCB 126, 0.1–3 µM PCB 126 or 3 µM TCDD for 6 hours or 4 hours, respectively. CYP1A1 mRNA expression was determined by qRT-PCR. Analysis was performed with normalization to RPLP0 mRNA. (A, D) Dose-response curves for PCB 126 treated cells in normal oxygen and hypoxia. The EC20 is indicated by a solid black line in normoxic cells and a dashed line in hypoxic cells. (B, C and E, F) CYP1A1 mRNA expression levels of untreated and treated cells including standard error. RNA expression levels significantly ( p

    Journal: Toxicology and applied pharmacology

    Article Title: Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines

    doi: 10.1016/j.taap.2013.12.002

    Figure Lengend Snippet: Oxygen concentration dependent induction of CYP1A1 mRNA expression HepG2 cells (left panel: A-C) and HaCaT cells (right panel: D-F) were incubated in normoxia (21% O 2 ) or hypoxia (1% O 2 ) for 8 hours prior to treatment with vehicle (DMSO), 2–4 nM PCB 126, 0.1–3 µM PCB 126 or 3 µM TCDD for 6 hours or 4 hours, respectively. CYP1A1 mRNA expression was determined by qRT-PCR. Analysis was performed with normalization to RPLP0 mRNA. (A, D) Dose-response curves for PCB 126 treated cells in normal oxygen and hypoxia. The EC20 is indicated by a solid black line in normoxic cells and a dashed line in hypoxic cells. (B, C and E, F) CYP1A1 mRNA expression levels of untreated and treated cells including standard error. RNA expression levels significantly ( p

    Article Snippet: As such, PCB 126 can bind to and activate the aryl hydrocarbon receptor (AhR), an immediate early cellular response to xenobiotics and mediator of many of the toxic effects of PCB exposure.

    Techniques: Concentration Assay, Expressing, Incubation, Quantitative RT-PCR, RNA Expression

    Hypoxia inhibits PCB 126 induced CYP1A1 promoter-luciferase reporter activity HepG2 cells (A) and HaCaT cells (B) were transfected with a XRE-luciferase (firefly) reporter vector and Renilla luciferase vector and then subjected to normoxia or hypoxia for 16 hours prior to treatment with 3 µM PCB 126 for 4 or 6 hours. Firefly luminescence was determined and normalized to Renilla luminescence, and is depicted relative to untreated cells in normoxia. * = p

    Journal: Toxicology and applied pharmacology

    Article Title: Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines

    doi: 10.1016/j.taap.2013.12.002

    Figure Lengend Snippet: Hypoxia inhibits PCB 126 induced CYP1A1 promoter-luciferase reporter activity HepG2 cells (A) and HaCaT cells (B) were transfected with a XRE-luciferase (firefly) reporter vector and Renilla luciferase vector and then subjected to normoxia or hypoxia for 16 hours prior to treatment with 3 µM PCB 126 for 4 or 6 hours. Firefly luminescence was determined and normalized to Renilla luminescence, and is depicted relative to untreated cells in normoxia. * = p

    Article Snippet: As such, PCB 126 can bind to and activate the aryl hydrocarbon receptor (AhR), an immediate early cellular response to xenobiotics and mediator of many of the toxic effects of PCB exposure.

    Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation

    ARNT overexpression relieves hypoxic inhibition of CYP1A1 promoter-luciferase reporter activity HepG2 cells were transfected with a XRE-luciferase (firefly) reporter vector, Renilla luciferase vector, and ARNT expression vector or empty control vector. Transfected cells were subjected to normoxia or hypoxia for 16 hours prior to treatment with 3 µM PCB 126 for 6 hours. Firefly luminescence was determined and is depicted relative to untreated cells in normoxia in the empty control vector group. * = p

    Journal: Toxicology and applied pharmacology

    Article Title: Hypoxia perturbs aryl hydrocarbon receptor signaling and CYP1A1 expression induced by PCB 126 in human skin and liver-derived cell lines

    doi: 10.1016/j.taap.2013.12.002

    Figure Lengend Snippet: ARNT overexpression relieves hypoxic inhibition of CYP1A1 promoter-luciferase reporter activity HepG2 cells were transfected with a XRE-luciferase (firefly) reporter vector, Renilla luciferase vector, and ARNT expression vector or empty control vector. Transfected cells were subjected to normoxia or hypoxia for 16 hours prior to treatment with 3 µM PCB 126 for 6 hours. Firefly luminescence was determined and is depicted relative to untreated cells in normoxia in the empty control vector group. * = p

    Article Snippet: As such, PCB 126 can bind to and activate the aryl hydrocarbon receptor (AhR), an immediate early cellular response to xenobiotics and mediator of many of the toxic effects of PCB exposure.

    Techniques: Over Expression, Inhibition, Luciferase, Activity Assay, Transfection, Plasmid Preparation, Expressing

    Incubations of PCB 118 in HLMs over time. Brackets indicate ±S.D.

    Journal: Environmental toxicology and pharmacology

    Article Title: The Role of African American Ethnicity and Metabolism in Sentinel Polychlorinated Biphenyl Congener Serum Levels

    doi: 10.1016/j.etap.2008.08.008

    Figure Lengend Snippet: Incubations of PCB 118 in HLMs over time. Brackets indicate ±S.D.

    Article Snippet: The loss of parent PCB 118 in CYP 3A4 incubations is not surprising since CYP 3A4 has a large active site and is known to accommodate the largest and most diverse set of xenobiotics in comparison to other isoforms [ ].

    Techniques: