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The Jackson Laboratory pbst
Pbst, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 94/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/The Jackson Laboratory
Average 94 stars, based on 136 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
94/100 stars

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Incubation:

Article Title: Adrenergic and noradrenergic innervation of the midbrain ventral tegmental area and retrorubral field: Prominent inputs from medullary homeostatic centers
Article Snippet: .. The sections were then rinsed 3 times in PBST and incubated in biotinylated donkey anti-rabbit-IgG (1/1000, Jackson Laboratories) for 2 h. After 3 rinses in 0.3 PBST, the sections were incubated in ABC (1/1000), rinsed once in PBST and twice in 0.05M TRIS buffer, and then reacted with 3,3′-diaminobenzidine tetrahydrochloride (0.02%, DAB; Sigma). .. This procedure yielded a brown reaction product that was easily distinguished from the blue of PMNT-positive fibers, allowing simultaneous labeling for both markers.

Article Title: Hypocretinergic and non-hypocretinergic projections from the hypothalamus to the REM sleep executive area of the pons
Article Snippet: .. After the sections were rinsed four times in PBST for a total duration of 30 min, they were simultaneously incubated for 3 hs in PBST containing donkey anti-rabbit IgG conjugated with Rhodamine (Jackson laboratories; 1;300) and donkey anti-goat IgG conjugated with fluorescein isothiocyanate (FITC, Jackson laboratories, 1:200). .. We also employed the reverse procedure; donkey anti-rabbit IgG conjugated with FITC (Jackson Laboratories: 1:200) and donkey anti-goat IgG conjugated with Rhodamine (Jackson Laboratories: 1:200).

Article Title: Inhibition of PTEN activates bovine non-growing follicles in vitro but increases DNA damage and reduces DNA repair response
Article Snippet: .. After washing with PBST (4 × 10 min), sections were incubated with appropriate secondary antibodies (Cy3-conjugated affinity pure donkey anti rabbit IgG [H + L], 1:250; Jackson Laboratories) for 2 hours and then washed for 2 × 10 min. .. The slides were then mounted in Vectashield hardset with 4′-6-diamidino-2-phenylindole (DAPI) (H-1500; Vector Laboratories).

Article Title: Nogo presence is inversely associated with shifts in cortical microglial morphology following experimental diffuse brain injury
Article Snippet: .. Sections were then rinsed three times in PBST and incubated for 1 hour in streptavidin bound AlexaFluor488 or AlexaFluor594 (Jackson Laboratories, cat #016-540-084 and cat #016-580-084, respectively, 1:1000) in PBS. .. Sections were then rinsed 3 times in PBST and dipped in Hoechst (Invitrogen, 1:1000) for 60 seconds, rinsed 3 more times in PBS then in ddH2 0 and cover slipped with Fluoromount-G (Southern Biotech, cat #0100-01).

Article Title: Mycobacterium tuberculosis extracellular vesicle-associated lipoprotein LpqH as a potential biomarker to distinguish paratuberculosis infection or vaccination from tuberculosis infection
Article Snippet: .. After washing three times with PBST, the wells were incubated with a horseradish peroxidase (HRP)-conjugated goat antibody to bovine immunoglobulin G (Jackson Laboratories) or an HRP-conjugated mouse immunoglobulin G (Sigma) for 1 h at 37 °C. .. Color was developed with TMB (Sigma-Aldrich).

Article Title: Adrenergic and noradrenergic innervation of the midbrain ventral tegmental area and retrorubral field: Prominent inputs from medullary homeostatic centers
Article Snippet: .. The sections were then rinsed 3 times in PBST and incubated in biotinylated donkey anti-rabbit-IgG (1/1000, Jackson Laboratories) for 2 h. After 3 rinses in PBST, the sections were incubated in ABC (1/1000), rinsed once in PBST and twice in 0.05M TRIS buffer, and then reacted with 3,3′-diaminobenzidine tetrahydrochloride (0.02%, DAB; Sigma). .. This procedure yielded a brown reaction product that was easily distinguished from the blue DBH-positive fibers, allowing simultaneous labeling for both markers.

Blocking Assay:

Article Title: Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach
Article Snippet: .. The blots were challenged with primary antibody at dilutions of 1/500-1/1000 in block overnight at 4 °C, followed by washing 3 times with PBST (1x PBS + 0.1% Tween 20) at room temperature and challenge with appropriate secondary antibody (1/5,000-1/10,000) conjugated to horseradish peroxidase (Jackson Laboratories) for 2 hours followed by washing 3 times with 1X PBST at room temperature. .. The blot was then visualized under chemiluminescence in an ECL Imager (Thermo Scientific) using Clarity Western ECL Substrate (Bio Rad Cat #170- 5061).

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    The Jackson Laboratory pbst
    Pbst, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 94/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/The Jackson Laboratory
    Average 94 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    94/100 stars
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