Structured Review

Merck KGaA pbst
Pbst, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Merck KGaA
Average 92 stars, based on 66 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Recombinant:

Article Title: The ears of the African elephant: unexpected high seroprevalence of Plasmodium ovale and Plasmodium malariae in healthy populations in Western Africa
Article Snippet: .. Native P. falciparum antigen produced from in vitro culture (NatELISA) [ ] or recombinant rMSP1 for P. ovale , P. malariae , P. falciparum and rP. falciparum AMA1 (RecELISA) were immobilized on 96-well plates overnight at 4°C in coating buffer and blocked for one hour with PBST (Phosphate Buffered Saline with Tween 0.05%) containing 1% BSA (bovine serum albumin) (Merck, Darmstadt, Germany). .. After washing with PBST, 200 μl of diluent buffer (PBST with BSA 0.1%) were dispensed into each well and 10 μl of serum were incubated for one hour at 37°C.

In Vitro:

Article Title: The ears of the African elephant: unexpected high seroprevalence of Plasmodium ovale and Plasmodium malariae in healthy populations in Western Africa
Article Snippet: .. Native P. falciparum antigen produced from in vitro culture (NatELISA) [ ] or recombinant rMSP1 for P. ovale , P. malariae , P. falciparum and rP. falciparum AMA1 (RecELISA) were immobilized on 96-well plates overnight at 4°C in coating buffer and blocked for one hour with PBST (Phosphate Buffered Saline with Tween 0.05%) containing 1% BSA (bovine serum albumin) (Merck, Darmstadt, Germany). .. After washing with PBST, 200 μl of diluent buffer (PBST with BSA 0.1%) were dispensed into each well and 10 μl of serum were incubated for one hour at 37°C.

Labeling:

Article Title: Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish
Article Snippet: .. After washing with PBST, embryos were incubated with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody at room temperature for 3 h. HRP labeling was visualized using DAB (Merck, Darmstadt, Germany) as a substrate. .. Nuclei were counterstained with hematoxylin (Merck, Darmstadt, Germany).

Produced:

Article Title: The ears of the African elephant: unexpected high seroprevalence of Plasmodium ovale and Plasmodium malariae in healthy populations in Western Africa
Article Snippet: .. Native P. falciparum antigen produced from in vitro culture (NatELISA) [ ] or recombinant rMSP1 for P. ovale , P. malariae , P. falciparum and rP. falciparum AMA1 (RecELISA) were immobilized on 96-well plates overnight at 4°C in coating buffer and blocked for one hour with PBST (Phosphate Buffered Saline with Tween 0.05%) containing 1% BSA (bovine serum albumin) (Merck, Darmstadt, Germany). .. After washing with PBST, 200 μl of diluent buffer (PBST with BSA 0.1%) were dispensed into each well and 10 μl of serum were incubated for one hour at 37°C.

Incubation:

Article Title: Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish
Article Snippet: .. After washing with PBST, embryos were incubated with a horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody at room temperature for 3 h. HRP labeling was visualized using DAB (Merck, Darmstadt, Germany) as a substrate. .. Nuclei were counterstained with hematoxylin (Merck, Darmstadt, Germany).

Article Title: A study on the biological function of heat shock factor 1 proteins in breast cancer
Article Snippet: .. After washing with PBS with Tween 20 (PBST) 3 times the next day, HRP-labeled secondary goat anti-rabbit (HRP) IgG antibody (dilution, 1/2,000; cat. no. ab6721, Abcam) was used for incubation at room temperature for 1 h. At the end of that, proteins were washed with PBST 3 times for 5 min, and then enhanced chemiluminescence (ECL) (Merck Millipore, Billerica, MA, USA) was used for color development with β-tubulin as an internal reference. .. Effects of HSF1 on tumorigenesis ability of MCF-7 cells in vivo The nude mice were embedded with estrogenic sustained release tablets (0.36 mg/tablet; Innovative Research, Novi, MI, USA) subcutaneously before inoculation of the tumor.

Article Title: ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons
Article Snippet: .. Free‐floating brain slices were washed with PBST, blocked in 5% donkey serum in PBST, and incubated overnight with primary antibodies (mouse anti‐OLIG2, 1:250, Merck Millipore, catalog number MABN50; rabbit anti‐OLIG2, Merck Millipore, catalog number AB9610; anti‐TCF7l2, 1:250, Merck Millipore, catalog number 05‐511; anti‐PDGFR alpha, Abcam, ab6121; anti‐OLIG1, Merck Millipore, MAB5540) at 4°C. .. On the next day, OPCs and brain slices were washing and they were incubated with Alexa Fluor‐conjugated secondary antibodies (anti‐Rabbit IgG (H + L), Alexa Fluor® 488 conjugate, ThermoFisher Scientific, A‐21206; anti‐Mouse IgG (H + L) Secondary Antibody, Alexa Fluor® 594 conjugate, ThermoFisher Scientific, A‐21203).

Article Title: Cucurbitacin I inhibits STAT3, but enhances STAT1 signaling in human cancer cells in vitro through disrupting actin filaments
Article Snippet: .. The precipitates were washed and resuspended in kinase reaction buffer (60 mmol/L HEPES, pH 7.5; 5 mmol/L MgCl2 ; 5 mmol/L MnCl2 ; 25 μmol/L Na3 VO4 ; and 200 μmol/L ATP) and were then incubated with DMSO, CuI or 2-MS for 15 min at room temperature, followed by incubation with the biotinylated peptide FLT3 (Cell Signaling Technology, Danvers, MA, USA) for 30 min at 30 °C; then, the supernatants were transferred to streptavidin-coated 96-well plates, incubated for 1 h at 30 °C, rinsed with 0.1% PBST (PBS+ 0.1% Tween-20) and incubated with the anti-phosphotyrosine antibody 4G10 (# 05-321MG, Merck Millipore, Billerica, MA, USA) for 2 h at 37 °C and with HRP-conjugated secondary antibody for 30 min at 30 °C; after five washes, the TMP substrate (# 7004S, Cell Signaling Technology, Danvers, MA, USA) was added, and the reaction was stopped by adding 2 mol/L H2 SO4 ; finally, the absorbance was measured at 450 nm. .. An in vitro phosphatase assay was performed with SHP2 or TCPTP immunoprecipitates.

Article Title: In Utero Bisphenol A Exposure Induces Abnormal Neuronal Migration in the Cerebral Cortex of Mice
Article Snippet: .. Briefly, brain tissue sections were washed in PBS containing 0.05% Triton X-100 (PBST) and fixed in 4% PFA for 10 min. After blocking with 3% bovine serum albumins in PBST at room temperature for 1 h, the brain sections were incubated with anti-MAP2 antibodies that were conjugated to Alexa Fluor 488 (Merck Millipore Japan Headquarters, Tokyo, Japan) for 3 h. Following additional washing with PBST, the sections were mounted with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA, USA). .. Images were acquired using a Leica microscope (DM6000 B, Leica Microsystems K.K.), processed with Neurolucida (MBF Bioscience) and Image-J (NIH) software.

Blocking Assay:

Article Title: In Utero Bisphenol A Exposure Induces Abnormal Neuronal Migration in the Cerebral Cortex of Mice
Article Snippet: .. Briefly, brain tissue sections were washed in PBS containing 0.05% Triton X-100 (PBST) and fixed in 4% PFA for 10 min. After blocking with 3% bovine serum albumins in PBST at room temperature for 1 h, the brain sections were incubated with anti-MAP2 antibodies that were conjugated to Alexa Fluor 488 (Merck Millipore Japan Headquarters, Tokyo, Japan) for 3 h. Following additional washing with PBST, the sections were mounted with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA, USA). .. Images were acquired using a Leica microscope (DM6000 B, Leica Microsystems K.K.), processed with Neurolucida (MBF Bioscience) and Image-J (NIH) software.

Western Blot:

Article Title: Biomass burning in the Amazon region causes DNA damage and cell death in human lung cells
Article Snippet: .. After final washes with PBST, the blots were developed using an enhanced chemiluminescence detection system (Immobilon Western, Merck Millipore). .. Protein expression was detected using anti-p53 (1:500, M7001, Dako Denmark A/S); anti-p21 (1:200, sc-56335, Santa Cruz, Santa Cruz, CA, USA); anti-LC3 (1:1000 dilution, L8918, Sigma-Aldrich); anti-GAPDH (1:1000 dilution, Santa Cruz, Santa Cruz, CA, USA) antibodies.

Binding Assay:

Article Title: Cortisol levels in skimmed milk during the first 22 weeks of lactation and response to short-term metabolic stress and lameness in dairy cows
Article Snippet: .. Residual binding sites were blocked with 380 μL/well 0.1 % BSA in PBST (phosphate-buffered saline with Tween-20), containing 7.12 g/L Na2 HPO4 × 2 H2 O, 8.5 g/L NaCl (both Merck KGaA, Darmstadt, Germany), 1 g/L BSA (Serva GmbH, Heidelberg, Germany), deionized water and HCl until pH 7.2 (Merck KGaA, Darmstadt, Germany). ..

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    Merck KGaA pbst
    Pbst, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Merck KGaA
    Average 92 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    92/100 stars
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