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Fisher Scientific pbst
Pbst, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Fisher Scientific
Average 93 stars, based on 77 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
93/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Longevity of adenovirus vector immunity in mice and its implications for vaccine efficacy
Article Snippet: .. The plates were washed 4 times with PBST (PBS + Tween 0.5%) and horseradish peroxidase-conjugated goat anti-mouse IgG (anti-mouse IgG-HRP) [Invitrogen|Thermo Fisher Scientific) at a dilution of 1:5000 in PBS containing 0.5% BSA was added into each well and incubated at room temperature for 2 h. The plates were washed 4 times with PBST, and the color development was achieved with BD OptEIA™ ELISA set TMB substrate (Thermo Fisher Scientific) following the manufacturer recommendations. ..

Staining:

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: .. After PBST washes, embryos were stained with diluted Hoechst 33342 (1:1000, Thermal Fisher Scientific) in PBST for 30 min. After PBST washes, 4% paraformaldehyde fixation, and more PBST washes, embryos were stored in 80% glycerol at 4°C. .. Immunofluorescence on chimeric embryos was conducted on fixed BrdU-exposed bud embryos that had been stored in 100% methanol at -20°C.

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: .. Embryos were then washed with PBST and blocked in 1% blocking reagent at room temperature for 1 h before incubation with anti-rabbit Alexa-647 antibody (1:200, Thermal Fisher Scientific) and anti-mouse Alexa-488 antibody (1:200) diluted in 0.5% blocking reagent at room temperature for 5 h. After PBST washes, cell nuclei were stained with Hoechst 33342 (1:1000) in PBST for 30 min. Embryos were then washed with PBST, fixed with 4% paraformaldehyde, more PBST washes, and stored in 80% glycerol at 4°C. .. Rescue experiments were conducted by co-injection of 3 ng each of klf4 -MO1 and MO2 with 50 pg klf4-7mm mRNA, 50 pg klf4ΔC-7mm mRNA, 9–12 ng of p53 MO or cdkn1a MO into 1–2 cell zygotes and embryos were allowed to develop to 80% epiboly stage before BrdU incubation.

Incubation:

Article Title: RNA-Mediated Thermoregulation of Iron-Acquisition Genes in Shigella dysenteriae and Pathogenic Escherichia coli
Article Snippet: .. After blocking, the membrane was washed 4 times for 5 minutes each time using PBST and then incubated for 1 hour at 4°C with polyclonal α-ShuA IgG (Custom antibody raised in a rabbit - Fisher Scientific, Waltham, MA) diluted 1:1000 in a solution of PBST with 5% milk. .. Following a series of washes as described above, the membrane was incubated for 1 hour at 4°C with goat anti-rabbit HRP conjugated IgG (Bio-Rad) diluted 1:10,000 in a solution of PBST with 5% milk.

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: .. Embryos were then washed with PBST and blocked in 1% blocking reagent at room temperature for 1 h before incubation with anti-rabbit Alexa-647 antibody (1:200, Thermal Fisher Scientific) and anti-mouse Alexa-488 antibody (1:200) diluted in 0.5% blocking reagent at room temperature for 5 h. After PBST washes, cell nuclei were stained with Hoechst 33342 (1:1000) in PBST for 30 min. Embryos were then washed with PBST, fixed with 4% paraformaldehyde, more PBST washes, and stored in 80% glycerol at 4°C. .. Rescue experiments were conducted by co-injection of 3 ng each of klf4 -MO1 and MO2 with 50 pg klf4-7mm mRNA, 50 pg klf4ΔC-7mm mRNA, 9–12 ng of p53 MO or cdkn1a MO into 1–2 cell zygotes and embryos were allowed to develop to 80% epiboly stage before BrdU incubation.

Article Title: Longevity of adenovirus vector immunity in mice and its implications for vaccine efficacy
Article Snippet: .. The plates were washed 4 times with PBST (PBS + Tween 0.5%) and horseradish peroxidase-conjugated goat anti-mouse IgG (anti-mouse IgG-HRP) [Invitrogen|Thermo Fisher Scientific) at a dilution of 1:5000 in PBS containing 0.5% BSA was added into each well and incubated at room temperature for 2 h. The plates were washed 4 times with PBST, and the color development was achieved with BD OptEIA™ ELISA set TMB substrate (Thermo Fisher Scientific) following the manufacturer recommendations. ..

Article Title: The rostral subcommissural ventral pallidum is a mix of ventral pallidal neurons and neurons from adjacent areas: an electrophysiological study
Article Snippet: .. Slices were then fixed in PBS containing 4 % paraformaldehyde for 1 h, washed with PBST and incubated for 1 h (room temperature) in PBST containing Bovine Serum Albumin (BSA, Fisher Scientific, Fair Lawn, NJ, USA). .. Slices were then incubated with anti-GAD67 (mouse, 1:500, Millipore Corporation, Billerica, MA, USA) for 72 h at 4 °C, washed and then incubated with the two secondary antibodies (streptavidin Alexa 488, 1:500, for neurobiotin; mouse Alexa 594, 1:500, for GAD67, Invitrogen Corporation, Carlsbad, CA, USA) for 2 h at room temperature.

Article Title: Entry Inhibition and Modulation of Pro-Inflammatory Immune Response Against Influenza A Virus by a Recombinant Truncated Surfactant Protein D
Article Snippet: .. The wells were washed again with PBST and incubated with anti-mouse IgG-Horseradish peroxidase (HRP)-conjugate (1:5,000) (Fisher Scientific) and Protein A-HRP-conjugate (Fisher Scientific) in PBS (100 µl/well), respectively, for 1 h at 37°C. .. Color was developed using 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Sigma-Aldrich).

Blocking Assay:

Article Title: RNA-Mediated Thermoregulation of Iron-Acquisition Genes in Shigella dysenteriae and Pathogenic Escherichia coli
Article Snippet: .. After blocking, the membrane was washed 4 times for 5 minutes each time using PBST and then incubated for 1 hour at 4°C with polyclonal α-ShuA IgG (Custom antibody raised in a rabbit - Fisher Scientific, Waltham, MA) diluted 1:1000 in a solution of PBST with 5% milk. .. Following a series of washes as described above, the membrane was incubated for 1 hour at 4°C with goat anti-rabbit HRP conjugated IgG (Bio-Rad) diluted 1:10,000 in a solution of PBST with 5% milk.

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: .. Embryos were then washed with PBST and blocked in 1% blocking reagent at room temperature for 1 h before incubation with anti-rabbit Alexa-647 antibody (1:200, Thermal Fisher Scientific) and anti-mouse Alexa-488 antibody (1:200) diluted in 0.5% blocking reagent at room temperature for 5 h. After PBST washes, cell nuclei were stained with Hoechst 33342 (1:1000) in PBST for 30 min. Embryos were then washed with PBST, fixed with 4% paraformaldehyde, more PBST washes, and stored in 80% glycerol at 4°C. .. Rescue experiments were conducted by co-injection of 3 ng each of klf4 -MO1 and MO2 with 50 pg klf4-7mm mRNA, 50 pg klf4ΔC-7mm mRNA, 9–12 ng of p53 MO or cdkn1a MO into 1–2 cell zygotes and embryos were allowed to develop to 80% epiboly stage before BrdU incubation.

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    Fisher Scientific pbst
    Pbst, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Fisher Scientific
    Average 93 stars, based on 77 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    91
    Fisher Scientific tween pbst
    Tween Pbst, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween pbst/product/Fisher Scientific
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    tween pbst - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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