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Developmental Studies Hybridoma Bank pbst
Pbst, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Developmental Studies Hybridoma Bank
Average 93 stars, based on 36 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
93/100 stars

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IA:

Article Title: The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease
Article Snippet: .. Tissues were blocked with 2% normal goat serum (NGS) in PBST and incubated overnight with mouse anti-Repo [1:10; 8D12, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA, USA], mouse anti-Chaoptin (1:200; 24B10, DSHB, Iowa City, IA, USA), mouse anti-Aβ42 (1:200; DE2B4, sc-58508, Santa Cruz Biotechnology, Dallas, TX, USA), rat anti-Elav (1:200; 7E8A10, DSHB, Iowa City, IA, USA) or rabbit anti-Sra (1:50; a gift from Dr Toshiro Aigaki, Tokyo Metropolitan University, Japan) antibodies at 4°C. .. The samples were then incubated with Alexa-Fluor-555-labeled anti-mouse, Alexa-Fluor-488-labeled anti-mouse, Alexa-Fluor-555-labeled anti-rabbit or Alexa-Fluor-594-labeled anti-rat secondary antibodies (1:200; Invitrogen, Carlsbad, CA, USA) for 1 h. For immunohistochemistry with adult brains, whole bodies of 3- to 5-day-old male flies were fixed in 4% paraformaldehyde containing 0.5% Triton X-100 at room temperature for 3 h. Whole brains were dissected out, blocked with 5% NGS and 2% bovine serum albumin in PBS containing 0.5% Triton X-100 for 3 h. They were then stained with mouse anti-Fasciclin II (1:200; 1D4, DSHB, Iowa City, IA, USA) or rabbit anti-Sra (1:50; a gift from Dr Toshiro Aigaki) at 4°C for 48 h. After washing four times with PBS containing 0.5% Triton X-100, samples were incubated at 4°C overnight with Alexa-Fluor-555-labeled anti-mouse or anti-rabbit antibodies (1:200; Invitrogen, Carlsbad, CA, USA).

Article Title: Deficiency in DNA damage response of enterocytes accelerates intestinal stem cell aging in Drosophila
Article Snippet: .. The following primary antibodies diluted in PBST were used in these experiments: mouse anti-Delta, mouse anti-Arm (Developmental Studies Hybridoma Bank, Iowa City, IA, USA), 1:200; mouse anti-GFP and rabbit anti-GFP (Molecular Probes, Eugene, OR, USA), 1:1000; rat anti-GFP (Nacalai Tesque Inc., Kyoto. .. Japan), 1:1000; rabbit anti-γH2AvD (Rockland, Gilbertsville, PA, USA) 1:2000; rabbit anti-pS/TQ (Cell Signaling Technologies, Danvers, MA, USA), 1:1000; rabbit anti-phospho-histone H3 (PH3, Millipore, Billerica, MA, USA), 1:1000; mouse anti-γ-tubulin (Sigma-Aldrich), 1:1000; rabbit anti-β-gal (Upstate Biotechnology Inc., Lake Placid, NY, USA), 1:1000; and anti-CCleaved caspase-3 (Cell Signaling Technologies), 1:1000; rabbit anti-pJNK antibody (Cell Signaling Technologies).

Staining:

Article Title: Morphological and biomechanical characterization of immature and mature nasoseptal cartilage
Article Snippet: .. 10% goat serum in PBST was used for blocking for 1 hour at room temperature prior to staining with type II collagen primary antibody (II-II6B3-s, 22 ug/ml) (DSHB): a mouse monoclonal antibody, 1:10 dilution) overnight at 4 °C. .. The sections were washed three times with PBST/TBST for 5 minutes prior to staining with secondary antibody goat anti-mouse IgG-FITC (Santa Cruz, sc-2010): 1:100 before washing for a further three times and adding SlowFade® Gold Antifade Mountant with DAPI (Thermofisher Waltham, MA, USA) and mounting coverslip.

Next-Generation Sequencing:

Article Title: The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease
Article Snippet: .. Tissues were blocked with 2% normal goat serum (NGS) in PBST and incubated overnight with mouse anti-Repo [1:10; 8D12, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA, USA], mouse anti-Chaoptin (1:200; 24B10, DSHB, Iowa City, IA, USA), mouse anti-Aβ42 (1:200; DE2B4, sc-58508, Santa Cruz Biotechnology, Dallas, TX, USA), rat anti-Elav (1:200; 7E8A10, DSHB, Iowa City, IA, USA) or rabbit anti-Sra (1:50; a gift from Dr Toshiro Aigaki, Tokyo Metropolitan University, Japan) antibodies at 4°C. .. The samples were then incubated with Alexa-Fluor-555-labeled anti-mouse, Alexa-Fluor-488-labeled anti-mouse, Alexa-Fluor-555-labeled anti-rabbit or Alexa-Fluor-594-labeled anti-rat secondary antibodies (1:200; Invitrogen, Carlsbad, CA, USA) for 1 h. For immunohistochemistry with adult brains, whole bodies of 3- to 5-day-old male flies were fixed in 4% paraformaldehyde containing 0.5% Triton X-100 at room temperature for 3 h. Whole brains were dissected out, blocked with 5% NGS and 2% bovine serum albumin in PBS containing 0.5% Triton X-100 for 3 h. They were then stained with mouse anti-Fasciclin II (1:200; 1D4, DSHB, Iowa City, IA, USA) or rabbit anti-Sra (1:50; a gift from Dr Toshiro Aigaki) at 4°C for 48 h. After washing four times with PBS containing 0.5% Triton X-100, samples were incubated at 4°C overnight with Alexa-Fluor-555-labeled anti-mouse or anti-rabbit antibodies (1:200; Invitrogen, Carlsbad, CA, USA).

Incubation:

Article Title: The calcineurin inhibitor Sarah (Nebula) exacerbates Aβ42 phenotypes in a Drosophila model of Alzheimer's disease
Article Snippet: .. Tissues were blocked with 2% normal goat serum (NGS) in PBST and incubated overnight with mouse anti-Repo [1:10; 8D12, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA, USA], mouse anti-Chaoptin (1:200; 24B10, DSHB, Iowa City, IA, USA), mouse anti-Aβ42 (1:200; DE2B4, sc-58508, Santa Cruz Biotechnology, Dallas, TX, USA), rat anti-Elav (1:200; 7E8A10, DSHB, Iowa City, IA, USA) or rabbit anti-Sra (1:50; a gift from Dr Toshiro Aigaki, Tokyo Metropolitan University, Japan) antibodies at 4°C. .. The samples were then incubated with Alexa-Fluor-555-labeled anti-mouse, Alexa-Fluor-488-labeled anti-mouse, Alexa-Fluor-555-labeled anti-rabbit or Alexa-Fluor-594-labeled anti-rat secondary antibodies (1:200; Invitrogen, Carlsbad, CA, USA) for 1 h. For immunohistochemistry with adult brains, whole bodies of 3- to 5-day-old male flies were fixed in 4% paraformaldehyde containing 0.5% Triton X-100 at room temperature for 3 h. Whole brains were dissected out, blocked with 5% NGS and 2% bovine serum albumin in PBS containing 0.5% Triton X-100 for 3 h. They were then stained with mouse anti-Fasciclin II (1:200; 1D4, DSHB, Iowa City, IA, USA) or rabbit anti-Sra (1:50; a gift from Dr Toshiro Aigaki) at 4°C for 48 h. After washing four times with PBS containing 0.5% Triton X-100, samples were incubated at 4°C overnight with Alexa-Fluor-555-labeled anti-mouse or anti-rabbit antibodies (1:200; Invitrogen, Carlsbad, CA, USA).

Article Title: A dPIP5K Dependent Pool of Phosphatidylinositol 4,5 Bisphosphate (PIP2) Is Required for G-Protein Coupled Signal Transduction in Drosophila Photoreceptors
Article Snippet: .. The tissues were then incubated in blocking solution [5% fetal bovine serum (FBS) in PBST] for 2 hours at room temperature, after which the tissues were incubated with primary antibodies diluted in blocking solution [anti-p-Moesin-1:200[ ]], anti-Rh1–1:50 (4C5, Developmental Studies Hybridoma Bank), anti-TRP-1:250,anti-dPIP5K-1:100, anti-GFP-1: 200 (Abcam)] overnight at 4°C. .. Appropriate secondary antibodies conjugated with a fluorophore were used at 1:300 dilutions [Alexa Fluor 633/568 IgG, (Molecular Probes)] and incubated for 2 hours at room temperature.

Article Title: Body wall development in lamprey and a new perspective on the origin of vertebrate paired fins
Article Snippet: .. Cryosections were blocked in PBST with 5% (vol/vol) normal goat serum and 2% (vol/vol) bovine serum albumin and incubated overnight with MF20 (1:20 dilution; DSHB) or DP312 (1:20 dilution). .. AlexaFluor 647 goat anti-mouse IgG2b (Invitrogen) fluorescent secondary was used with MF20.

Article Title: Contribution of sleep to the repair of neuronal DNA double-strand breaks: evidence from flies and mice
Article Snippet: .. Samples were then rinsed with PBST, incubated overnight at 4 °C with normal goat serum, and then reacted with a primary mouse antibody against gamma-H2AV (UNC93-5.2.1, 1:10 supernatant, DSHB;) and a rabbit antibody against GFP (A21311, 1:1000, Invitrogen). .. Secondary staining occurred with goat anti-mouse antibody labeled with 568 (A11004, Invitrogen), and goat anti-rabbit antibody labeled with 488 alexa fluor.

Article Title: Drosophila TIM Binds Importin α1, and Acts as an Adapter to Transport PER to the Nucleus
Article Snippet: .. After a 30min-wash with 1x PBST at room temperature, brains were blocked with 5% normal donkey serum (NDS) for 20 minutes, and then incubated overnight at 4°C with primary antibody: rat anti-PER (UPR34, 1:1000), anti-TIM (UPR42, 1:500), and mouse anti-PDF (C7, Developmental Studies Hybridoma Bank, 1:500). .. After a 30-min wash in 1× PBST at room temperature, brains were incubated with secondary antibodies (Jackson ImmunoResearch Laboratories, 1:500) for two hours in NDS at room temperature, followed by an extensive 30min-wash.

Blocking Assay:

Article Title: A dPIP5K Dependent Pool of Phosphatidylinositol 4,5 Bisphosphate (PIP2) Is Required for G-Protein Coupled Signal Transduction in Drosophila Photoreceptors
Article Snippet: .. The tissues were then incubated in blocking solution [5% fetal bovine serum (FBS) in PBST] for 2 hours at room temperature, after which the tissues were incubated with primary antibodies diluted in blocking solution [anti-p-Moesin-1:200[ ]], anti-Rh1–1:50 (4C5, Developmental Studies Hybridoma Bank), anti-TRP-1:250,anti-dPIP5K-1:100, anti-GFP-1: 200 (Abcam)] overnight at 4°C. .. Appropriate secondary antibodies conjugated with a fluorophore were used at 1:300 dilutions [Alexa Fluor 633/568 IgG, (Molecular Probes)] and incubated for 2 hours at room temperature.

Article Title: Morphological and biomechanical characterization of immature and mature nasoseptal cartilage
Article Snippet: .. 10% goat serum in PBST was used for blocking for 1 hour at room temperature prior to staining with type II collagen primary antibody (II-II6B3-s, 22 ug/ml) (DSHB): a mouse monoclonal antibody, 1:10 dilution) overnight at 4 °C. .. The sections were washed three times with PBST/TBST for 5 minutes prior to staining with secondary antibody goat anti-mouse IgG-FITC (Santa Cruz, sc-2010): 1:100 before washing for a further three times and adding SlowFade® Gold Antifade Mountant with DAPI (Thermofisher Waltham, MA, USA) and mounting coverslip.

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  • 93
    Developmental Studies Hybridoma Bank pbst
    Pbst, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Developmental Studies Hybridoma Bank
    Average 93 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Developmental Studies Hybridoma Bank bsa pbst
    Bsa Pbst, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa pbst/product/Developmental Studies Hybridoma Bank
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bsa pbst - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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