Structured Review

Beijing CWBio pbst
Pbst, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Beijing CWBio
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Staining:

Article Title: Pathogen-Specific Binding Soluble Down Syndrome Cell Adhesion Molecule (Dscam) Regulates Phagocytosis via Membrane-Bound Dscam in Crab
Article Snippet: .. After washing with PBST for removal of unbound antibody, cells were stained with goat anti-rat IgG H & L (FITC Conjugated) (1:100; CWBIO, China) and goat anti-mouse IgG H (Rhodamine Red-X Conjugated) (1:100; CWBIO, China) for 2 h at 37°C. .. After washing with PBST, cells were stained with DAPI for 1 min, and the cover slips were removed for observation under a fluorescence microscope (Olympus BX71, Japan).

Incubation:

Article Title: A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense.
Article Snippet: .. ASGPR (asialoglycoprotein receptor, also known as hepatic lectin) was the first identified animal lectin, which participated in a variety of physiological processes. .. ASGPR (asialoglycoprotein receptor, also known as hepatic lectin) was the first identified animal lectin, which participated in a variety of physiological processes.

Article Title: Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
Article Snippet: .. Next, the blots were incubated with rabbit polyclonal antibodies against human STC (1 : 500 in PBST; Abcam), Bcl-2 (1 : 600; Sangon Biotech), Bax (1 : 600; Sangon Biotech), caspase-3 (1 : 600; ABclonal Biotechnology, China), and GAPDH (1 : 5,000; Abcam) at RT for 2 h. The membranes were subsequently washed six times with PBST for 5 min at RT and then incubated with goat anti-rabbit IgG-HRP for 2 h at RT (1 : 8,000; CWbio). .. The immunoreactive bands were visualized using an Enhanced chemiluminescence (ECL) kit (CWbio).

other:

Article Title: Characterization of single-domain antibodies against Foot and Mouth Disease Virus (FMDV) serotype O from a camelid and imaging of FMDV in baby hamster kidney-21 cells with single-domain antibody-quantum dots probes
Article Snippet: The polyvinylidene fluoride (PVDF) membrane that carried the protein mixture was washed thrice with PBST and blocked with 5 % skim milk at 37 °C for 1 h. Horseradish peroxidase (HRP)-conjugated anti-His monoclonal antibody (diluted 1:1000, Cwbiotech, China) was recognized as the primary antibody.

Article Title: Target of Rapamycin Is a Key Player for Auxin Signaling Transduction in Arabidopsis
Article Snippet: The PVDF membrane was washed three times (10 min each) with PBST (Cwbio).

Article Title: Epidemic strain YC2014 of porcine epidemic diarrhea virus could provide piglets against homologous challenge
Article Snippet: After three washes with PBST, cells were treated with a rhodamine-conjugated goat anti-mouse IgG (Cwbio, China) at a 1:5000 dilution with PBS for 30 min at 37 °C.

Western Blot:

Article Title: Knockdown of PRL-3 increases mitochondrial superoxide anion production through transcriptional regulation of RAP1
Article Snippet: .. After washing with PBST three times, protein bands were visualized using an eECL WB kit (CWBIO, Beijing, China). .. In this study, the oxidation-sensitive fluorescent probes dihydroethidium (DHE) (GMS10111.1, Genmed, Plymouth, MN, USA), and MitoSOX Red (M36008, Thermo Fisher Scientific) were used to monitor the production of cytosolic superoxide anions and mitochondrial superoxide anions, respectively.

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    Beijing CWBio pbst
    Pbst, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Beijing CWBio
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    92/100 stars
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