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Becton Dickinson pbst
Pbst, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Becton Dickinson
Average 94 stars, based on 367 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
94/100 stars

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Related Articles

Cytometry:

Article Title: Genetic Variation and the Fate of Beneficial Mutations in Asexual Populations
Article Snippet: .. Cells were spun down and resuspended in cold PBST and the ratio of nonfluorescent (experimental) and mCherry-positive (reference) cells was determined by flow cytometry using an LSRII flow cytometer (BD Biosciences, San Jose, CA), counting 50,000 total cells for each sample. ..

Staining:

Article Title: Cyclosporin A Induces Cardiac Differentiation but Inhibits Hemato-Endothelial Differentiation of P19 Cells
Article Snippet: .. To dissociate EBs into single cells, EBs collected at 48 or 96 h were washed with PBS and incubated with accutase (Sigma-Aldrich A6964) at 37°C for 5 min. After PBS washing, the cells were fixed in 4% paraformaldehyde for 10 min, washed in PBST, and preblocked with 5% NGS in PBST for 1 h. The cells were stained with anti-Flk1 (1:200; BD Pharmingen 555307, San Jose, CA) antibody at 4°C overnight in 2% NGS in PBST, and then washed 3 times in PBST. .. The cells were stained with Alexa Fluor 488-conjugated anti-rat IgG (1:1000; Molecular Probes, Eugene, Oregon) antibody for 1 h and washed 3 times in PBST.

Next-Generation Sequencing:

Article Title: Cyclosporin A Induces Cardiac Differentiation but Inhibits Hemato-Endothelial Differentiation of P19 Cells
Article Snippet: .. To dissociate EBs into single cells, EBs collected at 48 or 96 h were washed with PBS and incubated with accutase (Sigma-Aldrich A6964) at 37°C for 5 min. After PBS washing, the cells were fixed in 4% paraformaldehyde for 10 min, washed in PBST, and preblocked with 5% NGS in PBST for 1 h. The cells were stained with anti-Flk1 (1:200; BD Pharmingen 555307, San Jose, CA) antibody at 4°C overnight in 2% NGS in PBST, and then washed 3 times in PBST. .. The cells were stained with Alexa Fluor 488-conjugated anti-rat IgG (1:1000; Molecular Probes, Eugene, Oregon) antibody for 1 h and washed 3 times in PBST.

Incubation:

Article Title: Both Ubiquitin Ligases FBXW8 and PARK2 Are Sequestrated into Insolubility by ATXN2 PolyQ Expansions, but Only FBXW8 Expression Is Dysregulated
Article Snippet: .. Membranes were blocked with 5% slim milk powder in PBST and incubated with primary antibodies against ATXN2 (1:400, BD Transduction Laboratories, 611378, monoclonal, host: mouse), FBXW8 (1:600, Pierce Antibodies, HPA038851, polyclonal, host: rabbit), PARK2 (1:800, Cell Signaling, 2132S, polyclonal, host: rabbit), or β-ACTIN (1:10,000, Sigma, A5441, monoclonal, host: mouse). .. Proteins were detected with SuperSignal West Pico (Thermo Scientific), with varying exposure times to avoid film sensitivity or saturation problems as well as non-linear effects.

Article Title: Bacterial Clearance Is Enhanced by α2,3- and α2,6-Sialyllactose via Receptor-Mediated Endocytosis and Phagocytosis
Article Snippet: .. After incubation for 2 h, plates were washed with PBST and then anti-CXCL8 antibodies and HRP were added (dilution, 1:250; BD Biosciences). ..

Article Title: Recombinant Mycobacterium smegmatis with a pMyong2 vector expressing Human Immunodeficiency Virus Type I Gag can induce enhanced virus-specific immune responses
Article Snippet: .. Plates were incubated for 2 hours at RT, washed three times with PBST and PBS, and incubated for 1 hour with mouse IgG2a and IgG1 antibody (BD Biosciences, 1:1,000 dilution). .. Thereafter, the plates were washed again and incubated with HRP conjugated streptavidin for 30 min at RT, and reacted with BD OptEIA substrate (BD Biosciences) for 10 min before stopping the reaction with 1N H2 SO4 .

Article Title: Cyclosporin A Induces Cardiac Differentiation but Inhibits Hemato-Endothelial Differentiation of P19 Cells
Article Snippet: .. To dissociate EBs into single cells, EBs collected at 48 or 96 h were washed with PBS and incubated with accutase (Sigma-Aldrich A6964) at 37°C for 5 min. After PBS washing, the cells were fixed in 4% paraformaldehyde for 10 min, washed in PBST, and preblocked with 5% NGS in PBST for 1 h. The cells were stained with anti-Flk1 (1:200; BD Pharmingen 555307, San Jose, CA) antibody at 4°C overnight in 2% NGS in PBST, and then washed 3 times in PBST. .. The cells were stained with Alexa Fluor 488-conjugated anti-rat IgG (1:1000; Molecular Probes, Eugene, Oregon) antibody for 1 h and washed 3 times in PBST.

Article Title: Hepatitis C VLPs Delivered to Dendritic Cells by a TLR2 Targeting Lipopeptide Results in Enhanced Antibody and Cell-Mediated Responses
Article Snippet: .. After washing with PBST (PBS containing 0.05% Tween 20), biotinylated anti-IFN-γ detection antibody (clone XMG1.2; Becton Dickinson, USA) was added and incubated for 2 hours at room temperature in a humidified atmosphere. .. Plates were then washed and streptavidin-alkaline phosphatase (Becton Dickinson, USA) added and incubated for a further 2 hours.

Article Title: Protection against Acute Hepatocellular Injury Afforded by Liver Fibrosis Is Independent of T Lymphocytes
Article Snippet: .. Membranes were washed 3 times for 10 minutes in PBST and then incubated with a secondary antibody (HRP-conjugated anti-rabbit IgG or anti-mouse IgG both from BD Pharmingen, CA) or anti-mouse IgM from anti-actin kit for 1 hour in PBST containing 5% milk at room temperature. .. Finally, after 3 washes in PBST, membranes were incubated in presence of enhanced chemiluminescence (ECL) reactive.

Flow Cytometry:

Article Title: Genetic Variation and the Fate of Beneficial Mutations in Asexual Populations
Article Snippet: .. Cells were spun down and resuspended in cold PBST and the ratio of nonfluorescent (experimental) and mCherry-positive (reference) cells was determined by flow cytometry using an LSRII flow cytometer (BD Biosciences, San Jose, CA), counting 50,000 total cells for each sample. ..

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    Becton Dickinson pbst
    Pbst, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Becton Dickinson
    Average 94 stars, based on 367 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Becton Dickinson tween 20 pbst
    Tween 20 Pbst, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbst/product/Becton Dickinson
    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbst - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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