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Agilent technologies pbst
Pbst, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Agilent technologies
Average 94 stars, based on 640 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
94/100 stars

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Incubation:

Article Title: Imaging of Cerebral Amyloid Angiopathy with Bivalent 99mTc-Hydroxamamide Complexes
Article Snippet: .. After three 5-min incubations in PBST, anti-rabbit secondary antibody (Dako, California, USA) incubation was carried out at room temperature for 3 h. Subsequently, the sections were incubated in PBST for 5 min three times, and incubated with diaminobenzidine (Merck) as a chromogen for 5 min. After washing with water, the sections were observed under a microscope (Keyence Corp.). ..

Article Title: Characterisation of the androgen regulation of glycine N-methyltransferase in prostate cancer cells
Article Snippet: .. The membrane was washed three times in PBST and incubated with the appropriate HRP-conjugated secondary antibody (Dako, Ely, UK) for 90 min at room temperature. ..

Article Title: Endothelin-1–Rho kinase interactions impair lung structure and cause pulmonary hypertension after bleomycin exposure in neonatal rat pups
Article Snippet: .. After rinsing, sections were covered in 10% horse serum (factor VII) or 10% goat serum (ETAR and ETBR) for 1 h and incubated with rabbit anti-factor VIII antibody (1:200) or sheep anti-ETAR, ETBR (1:150) diluted in PBST with 10% of the appropriate serum and 1% BSA overnight (no. A0082 Dako USA, Carpenteria, CA). .. After incubation, the sections were rinsed with PBST and incubated with HRP-labeled secondary antibody diluted 1:2,000 (factor VIII) or 1:500 (ETAR, ETBR) in PBST with 10% appropriate serum and 1% BSA for 1 h. After incubation with the secondary antibody, the sections were rinsed with PBST, incubated in ABC complex (Vector Laboratories, Burlingame, CA) for 30 min at room temperature, rinsed in PBST, and developed with diaminobenzidine and hydrogen peroxide (DAB kit, Vector SK-4100).

Article Title: Structure activity relationship of phenolic acid inhibitors of α-synuclein fibril formation and toxicity
Article Snippet: .. The membranes were then washed several times with PBST, followed by incubation with HRP-conjugated goat anti-mouse antibody (Dako Ltd., Ely, UK) at a dilution of 1:120,000 for 60 min at room temperature and gentle agitation. .. The immunoreactive bands were visualized with a SuperSignal West FemtoChemiluminescent Substrate Kit (Pierce, Rockford, USA) according to the manufacturer's instructions.

Article Title: Phosphokinase Antibody Arrays on Dendron-Coated Surface
Article Snippet: .. Before the hybridization, to reduce nonspecific binding, we added 200 µl of a blocking buffer with 3% Block Ace (SCETI K.K., Japan, Cat. UK-B80) in PBST to each chamber of Agilent 2 chamber gasket slides (Cat. G2534-60002) and then incubated the slides for 4 hrs with rotation. .. The slides were washed with PBST and incubated for 2 hrs with 25 µg of Cy3-labeled proteins in a final volume of 200 µl filled with PBST.

Article Title: High Resolution In Vivo Bioluminescent Imaging for the Study of Bacterial Tumour Targeting
Article Snippet: .. The sections were rinsed with PBST, and were incubated with Dako EnVision+ System – HRP Labelled Polymer Anti-Rabbit (Dako, K4003) for 30 minutes at RT. .. The sections were rinsed with PBST then incubated with DAB (3,3′-Diaminobenzidine) for visualisation.

Binding Assay:

Article Title: Phosphokinase Antibody Arrays on Dendron-Coated Surface
Article Snippet: .. Before the hybridization, to reduce nonspecific binding, we added 200 µl of a blocking buffer with 3% Block Ace (SCETI K.K., Japan, Cat. UK-B80) in PBST to each chamber of Agilent 2 chamber gasket slides (Cat. G2534-60002) and then incubated the slides for 4 hrs with rotation. .. The slides were washed with PBST and incubated for 2 hrs with 25 µg of Cy3-labeled proteins in a final volume of 200 µl filled with PBST.

Microscopy:

Article Title: Imaging of Cerebral Amyloid Angiopathy with Bivalent 99mTc-Hydroxamamide Complexes
Article Snippet: .. After three 5-min incubations in PBST, anti-rabbit secondary antibody (Dako, California, USA) incubation was carried out at room temperature for 3 h. Subsequently, the sections were incubated in PBST for 5 min three times, and incubated with diaminobenzidine (Merck) as a chromogen for 5 min. After washing with water, the sections were observed under a microscope (Keyence Corp.). ..

Blocking Assay:

Article Title: Drosophila Tet Is Expressed in Midline Glia and Is Required for Proper Axonal Development
Article Snippet: .. Next, brains were placed in blocking solution [5% normal goat serum (Dako) in PBST] overnight at 4°C. .. The following antibodies were used: rabbit anti-GFP (Abcam, 1:2000), mouse anti-Wrapper [10D3, Developmental Studies Hybridoma Bank (DSHB), 1:20], mouse anti-Slit (DSHB, C555.6D, 1:50), mouse anti-FasIII (7G10, DSHB, 1:30), mouse anti-Prospero (DSHB, MR1A, 1:100).

Article Title: Size-Dependent Deposition, Translocation, and Microglial Activation of Inhaled Silver Nanoparticles in the Rodent Nose and Brain
Article Snippet: .. Sections were then cooled for 15 min, rinsed with PBST for 3 min and with 3% hydrogen peroxide for 5 min, washed in PBST three times at 3 min each; the PBST washes were followed by a nonspecific block, Protein Block (Dako, Carpinteria, CA), for 30 min at 20°C. .. The sections were then incubated with the primary antibody, anti-ionized calcium-binding adapter molecule 1 (anti-Iba1; Abcam Inc., Cambridge, MA), for 3 hr to visualize microglial cells in a state of rest or activation or with anti-tumor necrosis factor alpha (anti-TNF-α; Abcam Inc.) for 1 hr at 20°C.

Article Title: Phosphokinase Antibody Arrays on Dendron-Coated Surface
Article Snippet: .. Before the hybridization, to reduce nonspecific binding, we added 200 µl of a blocking buffer with 3% Block Ace (SCETI K.K., Japan, Cat. UK-B80) in PBST to each chamber of Agilent 2 chamber gasket slides (Cat. G2534-60002) and then incubated the slides for 4 hrs with rotation. .. The slides were washed with PBST and incubated for 2 hrs with 25 µg of Cy3-labeled proteins in a final volume of 200 µl filled with PBST.

Hybridization:

Article Title: Phosphokinase Antibody Arrays on Dendron-Coated Surface
Article Snippet: .. Before the hybridization, to reduce nonspecific binding, we added 200 µl of a blocking buffer with 3% Block Ace (SCETI K.K., Japan, Cat. UK-B80) in PBST to each chamber of Agilent 2 chamber gasket slides (Cat. G2534-60002) and then incubated the slides for 4 hrs with rotation. .. The slides were washed with PBST and incubated for 2 hrs with 25 µg of Cy3-labeled proteins in a final volume of 200 µl filled with PBST.

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    Agilent technologies pbst
    Pbst, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Agilent technologies
    Average 94 stars, based on 640 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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