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Abcam pbst
r Ts -Pmy inhibited the binding of human <t>C1q</t> to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with <t>PBST,</t> the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p
Pbst, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1227 article reviews
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94/100 stars

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1) Product Images from "Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation"

Article Title: Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0004310

r Ts -Pmy inhibited the binding of human C1q to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p
Figure Legend Snippet: r Ts -Pmy inhibited the binding of human C1q to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p

Techniques Used: Binding Assay, Incubation

Classical complement activation was inhibited by r Ts -Pmy. Two μg of human C1q was pre-incubated with increasing amounts of r T s-Pmy (0, 2, 4 μg) or BSA (4 μg), then added to human IgM-coated plates. After washing with PBST for three times, a total of 2% C1q-deficient serum (C1q D) was added as a source of rest complement components. The deposition of C3 was detected with anti-C3 polyclonal antibody. The NHS alone and BSA added to the activation were used as controls. The results are shown as the means ± SD for three independent experiments.* p
Figure Legend Snippet: Classical complement activation was inhibited by r Ts -Pmy. Two μg of human C1q was pre-incubated with increasing amounts of r T s-Pmy (0, 2, 4 μg) or BSA (4 μg), then added to human IgM-coated plates. After washing with PBST for three times, a total of 2% C1q-deficient serum (C1q D) was added as a source of rest complement components. The deposition of C3 was detected with anti-C3 polyclonal antibody. The NHS alone and BSA added to the activation were used as controls. The results are shown as the means ± SD for three independent experiments.* p

Techniques Used: Activation Assay, Incubation

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Immunohistochemistry:

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Article Snippet: .. After being washed with PBST, the sections were stained using the mouse and rabbit-specific HRP/DAB (ABC) detection IHC kit (Abcam, ab64264) and analyzed using an Olympus BX60 compound microscope (Tokyo, Japan). .. Western Blot Analysis Lung proteins (n = 3 per group) were extracted from 30 mg lung tissue with 600 μL lysis buffer (Beyotime, China) and 6 μL protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, United States) on ice, and then centrifuged and collected.

Next-Generation Sequencing:

Article Title: The Anatomical Boundary of the Rat Claustrum
Article Snippet: .. Sections were incubated overnight, washed in PBST and incubated with the relevant secondary antibody (goat anti-mouse Alexa Fluor 488/594 (Abcam, Cambridge, United Kingdom) in 1% NGS 1:200 PBST. .. Microscopy and Imaging Brain sections were imaged in brightfield at 20× magnification using a Leica slide scanner (Aperio AT2), visualized in Aperio ImageScope (version 12.3.2.8013).

Microscopy:

Article Title: Regulation of Wnt Singaling Pathway by Poly (ADP-Ribose) Glycohydrolase (PARG) Silencing Suppresses Lung Cancer in Mice Induced by Benzo(a)pyrene Inhalation Exposure
Article Snippet: .. After being washed with PBST, the sections were stained using the mouse and rabbit-specific HRP/DAB (ABC) detection IHC kit (Abcam, ab64264) and analyzed using an Olympus BX60 compound microscope (Tokyo, Japan). .. Western Blot Analysis Lung proteins (n = 3 per group) were extracted from 30 mg lung tissue with 600 μL lysis buffer (Beyotime, China) and 6 μL protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, United States) on ice, and then centrifuged and collected.

Incubation:

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Article Title: The Anatomical Boundary of the Rat Claustrum
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Blocking Assay:

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Staining:

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Article Snippet: .. After being washed with PBST, the sections were stained using the mouse and rabbit-specific HRP/DAB (ABC) detection IHC kit (Abcam, ab64264) and analyzed using an Olympus BX60 compound microscope (Tokyo, Japan). .. Western Blot Analysis Lung proteins (n = 3 per group) were extracted from 30 mg lung tissue with 600 μL lysis buffer (Beyotime, China) and 6 μL protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, United States) on ice, and then centrifuged and collected.

Binding Assay:

Article Title: Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation
Article Snippet: .. After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody (Abcam, USA; 1:3,000 in 1% BSA/PBS). .. HRP-conjugated goat anti-rabbit IgG (BD Biosciences, USA) was used as the secondary antibody and OPD (Sigma, USA) was used as the substrate.

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  • pbst  (Abcam)
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    Abcam pbst
    r Ts -Pmy inhibited the binding of human <t>C1q</t> to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with <t>PBST,</t> the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p
    Pbst, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Abcam
    Average 94 stars, based on 1227 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    91
    Abcam 1x pbst
    r Ts -Pmy inhibited the binding of human <t>C1q</t> to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with <t>PBST,</t> the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p
    1x Pbst, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x pbst/product/Abcam
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    1x pbst - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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    r Ts -Pmy inhibited the binding of human C1q to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation

    doi: 10.1371/journal.pntd.0004310

    Figure Lengend Snippet: r Ts -Pmy inhibited the binding of human C1q to IgM. One μg of C1q was pre-incubated with different amounts of r Ts -Pmy or BSA (0, 2, 3, 4 μg), then added to the IgM coated plates. After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody. The results shown are means ± SD and are representative of three independent experiments. *** p

    Article Snippet: After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody (Abcam, USA; 1:3,000 in 1% BSA/PBS).

    Techniques: Binding Assay, Incubation

    Classical complement activation was inhibited by r Ts -Pmy. Two μg of human C1q was pre-incubated with increasing amounts of r T s-Pmy (0, 2, 4 μg) or BSA (4 μg), then added to human IgM-coated plates. After washing with PBST for three times, a total of 2% C1q-deficient serum (C1q D) was added as a source of rest complement components. The deposition of C3 was detected with anti-C3 polyclonal antibody. The NHS alone and BSA added to the activation were used as controls. The results are shown as the means ± SD for three independent experiments.* p

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation

    doi: 10.1371/journal.pntd.0004310

    Figure Lengend Snippet: Classical complement activation was inhibited by r Ts -Pmy. Two μg of human C1q was pre-incubated with increasing amounts of r T s-Pmy (0, 2, 4 μg) or BSA (4 μg), then added to human IgM-coated plates. After washing with PBST for three times, a total of 2% C1q-deficient serum (C1q D) was added as a source of rest complement components. The deposition of C3 was detected with anti-C3 polyclonal antibody. The NHS alone and BSA added to the activation were used as controls. The results are shown as the means ± SD for three independent experiments.* p

    Article Snippet: After washing three times with PBST, the binding of C1q to IgM was determined with anti-C1q polyclonal antibody (Abcam, USA; 1:3,000 in 1% BSA/PBS).

    Techniques: Activation Assay, Incubation