Structured Review

SouthernBiotech pbs t
Pbs T, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/SouthernBiotech
Average 93 stars, based on 171 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
93/100 stars

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Related Articles

Western Blot:

Article Title: Codon harmonization reduces amino acid misincorporation in bacterially expressed P. falciparum proteins and improves their immunogenicity
Article Snippet: .. Western blots were probed with Pf CelTOS-specific mouse mAbs 3D11.D4, 4H9.C3 and 3C3.B3 and anti-Histidine mouse mAb (at 1:3000 each) (Takara Bio, Mountain View, CA) for 1 h. After washing with PBS-T, blots were probed with alkaline phosphatase (AP)-conjugated anti-mouse IgG (1:5000) (Southern Biotech, Birmingham, AL). .. Blots were washed with PBS-T and developed for 10 min at RT with 4-nitro-blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (BCIP) (Roche, Branchburg, NJ) in 0.1 M sodium chloride, 0.005 M magnesium chloride and 0.1 M Tris–HCl pH 9.

Incubation:

Article Title: New monoclonal anti-mouse DC-SIGN antibodies reactive with acetone-fixed cells
Article Snippet: .. Blotted membranes were blocked with 10 % non-fat dry milk in PBS-T, incubated with serum/supernatant/antibody samples, detected by anti-rat, anti-mouse, or anti-rabbit IgG secondary antibodies conjugated with HRP (Southern Biotech, Birmingham, AL), and visualized with ECL Plus™ reagents (GE Healthcare). .. After detaching with 1 mM EDTA in PBS for 10 min, stable CHO cells were incubated with primary Abs for 30 min at 4 °C.

Article Title: Maternal immunization confers protection to the offspring against an attaching and effacing pathogen through delivery of IgG in breast milk
Article Snippet: .. Wells were washed with PBS-T, and then incubated 1 h with goat alkaline phosphatase (AP)-conjugated anti-mouse IgG, IgA or IgM (1:500; SouthernBiotech). .. Binding of specific IgG subtypes or IgA was detected by measuring the O.D. at 405 nm after adding p-nitrophenyl phosphate (pNPP) substrate (SouthernBiotech).

Article Title: Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression
Article Snippet: .. After washing three times with PBS-T (0.05% tween-20 in PBS; Sigma), each well was blocked with a blocking buffer (0.5% BSA in PBS-T; genDEPOT, Katy, TX, USA) for 1 h. After removing the blocking buffer, the culture medium to be analyzed was added and allowed to react for 1 h. After washing three times with PBS-T, Goat Anti-Type I Collagen-BIOT (SouthernBiotech) was added and allowed to react for 1 h. After incubation with streptavidin conjugated horseradish peroxidase (Sigma) for 1 h, 3,3′,5,5′-tetramethylbenzidine (Sigma) was added and allowed to react for 20 min in the dark. .. Absorbance was measured with a Synergy HTX Multi-Mode Reader (BioTek Instruments Inc., Winooski, VT, USA) at a 450 nm wavelength.

Article Title: Maternal Immunization with Pneumococcal Surface Protein A Protects against Pneumococcal Infections among Derived Offspring
Article Snippet: .. To determined PspA specific antibody isotypes, after washing with PBS-T, the plate was incubated with 50 µl of 1/3000 biotinylated antibody to mouse IgG, IgA or IgM (Southern Biotechnology Associates, Birmingham, AL, USA) diluted in casein buffer for 2 h at room temperature, respectively. .. Then, after washing with PBS-T, the plate was incubated with 1/4000 alkaline phosphatase conjugated streptavidin (Southern Biotechnology Associates) for 2 h at room temperature.

Article Title: Serotype-Independent Protection Against Invasive Pneumococcal Infections Conferred by Live Vaccine With lgt Deletion
Article Snippet: .. The membrane was then washed with PBS-T and incubated with a horseradish peroxidase-conjugated rabbit anti-mouse immunoglobulin (Southern Biotech, Birmingham, AL, USA). .. Membrane-bound peroxidase was detected with 3,3′,5,5′-tetramethylbenzidine substrate solution (Thermo-Fisher Scientific, Waltham, MA, USA).

Immunostaining:

Article Title: Smad4 loss promotes lung cancer formation but increases sensitivity to DNA topoisomerase inhibitors
Article Snippet: .. Cells were then rinsed with PBS-T and mounted using DAPI Fluoromount-G (Southern Biotech, Birmingham, AL). pH2AX immunostaining was quantified by counting at least 100 nuclei per treatment condition; differences were compared by Chi-squared test. .. Annexin V flow cytometry Beas2B cells with Smad4 knockdown were treated with 10μM camptothecin for 4h then harvested with TrypLE (Life Technologies) and assayed with the Dead Cell Apoptosis Kit (Life Technologies) per the manufacturer's instructions using a FC500 flow cytometer (BD Biosciences, San Jose, CA).

Blocking Assay:

Article Title: Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression
Article Snippet: .. After washing three times with PBS-T (0.05% tween-20 in PBS; Sigma), each well was blocked with a blocking buffer (0.5% BSA in PBS-T; genDEPOT, Katy, TX, USA) for 1 h. After removing the blocking buffer, the culture medium to be analyzed was added and allowed to react for 1 h. After washing three times with PBS-T, Goat Anti-Type I Collagen-BIOT (SouthernBiotech) was added and allowed to react for 1 h. After incubation with streptavidin conjugated horseradish peroxidase (Sigma) for 1 h, 3,3′,5,5′-tetramethylbenzidine (Sigma) was added and allowed to react for 20 min in the dark. .. Absorbance was measured with a Synergy HTX Multi-Mode Reader (BioTek Instruments Inc., Winooski, VT, USA) at a 450 nm wavelength.

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  • 93
    SouthernBiotech pbs t
    Pbs T, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/SouthernBiotech
    Average 93 stars, based on 171 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    SouthernBiotech tween 20 pbs t
    Tween 20 Pbs T, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbs t/product/SouthernBiotech
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbs t - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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