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Santa Cruz Biotechnology pbs t
Pbs T, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Santa Cruz Biotechnology
Average 93 stars, based on 69 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
93/100 stars

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Incubation:

Article Title: Characterization of HCV Interactions with Toll-Like Receptors and RIG-I in Liver Cells
Article Snippet: .. After washing thrice with PBS-T for 10 minutes each, the membrane was incubated with the appropriate HRP-conjugated secondary antibody (all from Santa Cruz Biotechnology), diluted in PBS-T, for 1 hour at room temperature and washed 3 more times. .. Proteins were visualized with Supersignal West Pico Chemiluminescent Substrate (Pierce Biotechnology, Inc, Rockford, IL).

Article Title: Deletion of P2Y2 receptor reveals a role for lymphotoxin-α in fatty streak formation
Article Snippet: .. Tissue segments were then rinsed 3 times with PBS-T and incubated overnight at 4 °C with anti-mouse VCAM-1 polyclonal antibodies (Santa Cruz Biotechnology Inc; 1:100) diluted in PBS-T. Anti-donkey secondary antibody labeled with Alexa Fluor fluorophore molecules (Molecular Probes Inc.) was then applied for 45 min at room temperature. .. The segments were mounted on slides, covered with Vectorshield mounting medium (Vector Laboratories Inc) and observed with a FV1000 confocal system (Olympus).

Article Title: Protective Effect of Right Ventricular Mitochondrial Damage by Cyclosporine A in Monocrotaline-induced Pulmonary Hypertension
Article Snippet: .. Membranes were blocked with 5% milk in PBS-T for 1 hour and incubated overnight at 4°C with anti-voltage-dependent anion channel (VDAC) rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-adenine nucleotide translocator 1 (ANT1) goat polyclonal antibody (Santa Cruz Biotechnology, Inc.), rabbit anti-cyclophilin D (CypD) (Santa Cruz Biotechnology, Inc.) using mitochondrial fraction, anti-AIF goat polyclonal antibody (Santa Cruz Biotechnology, Inc.), and anti-caspase-3 (Casp3) rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc.) using cytosolic fraction. .. The sites of antibody-antigen reaction were visualized with horse radish peroxidase (HRP)-conjugated secondary antibodies (P447 or P448; Dako, Glostrup, Denmark), an enhanced chemiluminescence (ECL; Amersham Pharmacia Biotech, Little Chalfont, UK) system, and exposure to photographic film (Hyperfilm ECL, RPN3103K; Amersham Pharmacia Biotech).

Article Title: Tailored ss-Cyclodextrin Blocks the Translocation Pores of Binary Exotoxins from C. Botulinum and C. Perfringens and Protects Cells from Intoxication
Article Snippet: .. After washing with PBS-T, the membrane was incubated with an anti-rabbit antibody coupled to horseradish peroxidase (Santa-Cruz, Heidelberg, Germany), and then washed again. ..

Article Title: Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse
Article Snippet: .. After blocking, oocytes and embryos were incubated overnight with the primary antibody diluted to 1:100 in PBS-T. Second step: oocytes and embryos were then washed four times, for a total time of 15 min, in PBS-T and placed in the solution containing the secondary antibody [goat anti-rabbit fluorescein isothiocyanate (FITC)-conjugated antibody, Santa Cruz Biotech, diluted 1:100 in PBS-T] for 4 h. Oocytes and embryos were washed four times, for 15 min, in PBS-T. .. The Ob primary rabbit antibody was raised against the N-terminal region of the Ob gene product of human and to a lesser extent, mouse and rat (sc-842; Santa Cruz Biotech.

Article Title: Cloning and Expression of Major Surface Antigen 1 Gene of Toxoplasma gondii RH Strain Using the Expression Vector pVAX1 in Chinese Hamster Ovary Cells
Article Snippet: .. The cells were incubated with 1:100 diluted mouse anti-T. gondii p30 monoclonal antibody (Abcam, USA) diluted at room temperature for 60 minutes and washed three times by PBS-T, followed by incubation in 1:300 diluted FITC-labeled goat antimouse IgG antibody (Santa Cruz Biotechnology, Inc., USA) at room temperature for 60 minutes. .. After washing three times with PBS-T, incubation was performed in dark with DAPI (4', 6-Diamidino-2-phenylindole) at RT for 5 minutes and monolayers were covered with glycerine and examined for specific fluorescence under an Olympus IX70 multi-parameter fluorescence microscope (Olympus, Tokyo, Japan) ( ).

Article Title: Generation of X-CGD cells for vector evaluation from healthy donor CD34+ HSCs by shRNA-mediated knock down of gp91phox
Article Snippet: .. Equal amounts of protein per sample (30 μg) were run on 8% SDS polyacrylamide gels and electroblotted onto PVDF membranes that had been blocked using 4% milk powder in PBS-T. Membranes were incubated overnight at 4 °C with primary antibodies (mouse α-human gp91phox MoAB48, 1:500; rabbit α-actin, Santa Cruz), and peroxidase-conjugated secondary antibodies were used for detection (goat α-mouse/rabbit HRP, Santa Cruz, 1:5,000, 60 minutes, RT) in combination with the ECL detection kit Supersignal West Pico Chemiluminescent Substrate (Pierce, Rockford, IL). .. Cytochrome C reduction assay Differentiated cell populations were subjected to analysis as described before using a Spectra MAX 340 reader (Molecular Devices, Sunnyvale, CA) and the SOFTmax Version 2.02 PRO software.

Article Title: In Silico and Biochemical Characterization of Lysozyme-Like Proteins in the Rat
Article Snippet: .. The plates were then blocked with 1 mg/ml BSA for 2 h and varying concentrations of the recombinant LYZL protein was added to the wells and incubated for 3 h. The wells were then washed 4 times with PBS-T (PBS with 0.1% Tween-20), followed by sequential incubation with primary antibody against the LYZL protein being tested and Goat anti-Rabbit IgG-HRP conjugated antibody (Santa Cruz Biotechnology, Dallas, USA; sc-2054 RRID: AB_631748). .. After thorough washing, O-Phenylenediamine (OPD; Sigma Aldrich, St. Louis, USA) was used to measure the amount of antibody bound to the protein complex and the binding efficiency is measured in terms of ELISA index (EI).

Recombinant:

Article Title: In Silico and Biochemical Characterization of Lysozyme-Like Proteins in the Rat
Article Snippet: .. The plates were then blocked with 1 mg/ml BSA for 2 h and varying concentrations of the recombinant LYZL protein was added to the wells and incubated for 3 h. The wells were then washed 4 times with PBS-T (PBS with 0.1% Tween-20), followed by sequential incubation with primary antibody against the LYZL protein being tested and Goat anti-Rabbit IgG-HRP conjugated antibody (Santa Cruz Biotechnology, Dallas, USA; sc-2054 RRID: AB_631748). .. After thorough washing, O-Phenylenediamine (OPD; Sigma Aldrich, St. Louis, USA) was used to measure the amount of antibody bound to the protein complex and the binding efficiency is measured in terms of ELISA index (EI).

Labeling:

Article Title: Deletion of P2Y2 receptor reveals a role for lymphotoxin-α in fatty streak formation
Article Snippet: .. Tissue segments were then rinsed 3 times with PBS-T and incubated overnight at 4 °C with anti-mouse VCAM-1 polyclonal antibodies (Santa Cruz Biotechnology Inc; 1:100) diluted in PBS-T. Anti-donkey secondary antibody labeled with Alexa Fluor fluorophore molecules (Molecular Probes Inc.) was then applied for 45 min at room temperature. .. The segments were mounted on slides, covered with Vectorshield mounting medium (Vector Laboratories Inc) and observed with a FV1000 confocal system (Olympus).

Blocking Assay:

Article Title: Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse
Article Snippet: .. After blocking, oocytes and embryos were incubated overnight with the primary antibody diluted to 1:100 in PBS-T. Second step: oocytes and embryos were then washed four times, for a total time of 15 min, in PBS-T and placed in the solution containing the secondary antibody [goat anti-rabbit fluorescein isothiocyanate (FITC)-conjugated antibody, Santa Cruz Biotech, diluted 1:100 in PBS-T] for 4 h. Oocytes and embryos were washed four times, for 15 min, in PBS-T. .. The Ob primary rabbit antibody was raised against the N-terminal region of the Ob gene product of human and to a lesser extent, mouse and rat (sc-842; Santa Cruz Biotech.

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  • 93
    Santa Cruz Biotechnology pbs t
    Pbs T, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Santa Cruz Biotechnology
    Average 93 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology tween 20 pbs t
    Tween 20 Pbs T, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbs t/product/Santa Cruz Biotechnology
    Average 93 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbs t - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology pbs t buffer
    Pbs T Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t buffer/product/Santa Cruz Biotechnology
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pbs t buffer - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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