Structured Review

Proteintech pbs t
Pbs T, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Proteintech
Average 92 stars, based on 13 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
92/100 stars

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Protein Binding:

Article Title: FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo
Article Snippet: .. Samples were blocked for non-specific protein binding with 5% normal goat serum in PBS-T for 1 hour and incubated with anti-bFIZ1 antibody (1:100, 4°C), a rabbit IgG made to target domain-II-IV of FIZ1 (Proteintech Group Inc., Chicago, IL, USA) that was affinity purified and specific for FIZ1 in immunoblots of mammalian retina [ , ]. .. After washing with PBS-T (3 × 5 min), samples were incubated with Nanogold-Fab'goat anti-rabbit-IgG nano gold (gold particle size 1.5 nm maximum, 1.4 nm average), 1:50 in PBS-T, 1 hour at 37°C (Nanoprobes, Yaphank, NY).

Infection:

Article Title: Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells
Article Snippet: .. Successively, the membranes were blocked with 1% bovine serum albumin in phosphate-buffered saline (PBS) overnight at 4°C, washed five times with PBS-0.05% Tween 20 (PBS-T, pH 7.4), and incubated with His-tag monoclonal antibody, sera from naïve rats, rCs severin immunized rats, C. sinensis -infected rats or Cs ESPs immunized rats (1∶100 dilutions) followed by HRP-conjugated goat anti-mouse/rat IgG (Proteintech; dilution of 1∶2,000) at 37°C for 2 h. After adequately washing with PBS-T, the membrane was incubated with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG in 1∶2000 dilutions (Proteintech, USA) at 37°C for 1 h. Detection was then carried out by enhanced chemiluminescence (ECL) method. .. Expression level of Cs severin at life-stages of C. sinensis Intact living adult worms were collected from biliary tracts of infected cats and washed extensively and gently in physiological saline to remove any contamination from hosts.

Incubation:

Article Title: Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells
Article Snippet: .. The membrane was blocked with 1% bovine serum albumin in PBS overnight at 4°C, washed with PBS-T, and incubated with anti-Cs severin rat serum (1∶100 dilutions) or pre-immune rat serum (1∶100 dilutions) at 37°C for 2 h. After extensively washing with PBS-T, the membrane was incubated with HRP-conjugated goat anti-rat IgG in 1∶2000 dilutions (Proteintech, USA) at 37°C for 1 h. Detection was then carried out by ECL. .. Immunohistochemical localization of Cs severin in C. sinensis adults and metacercariae Fresh adult worms and metacercariae of C. sinensis were fixed with 4% formaldehyde, embedded with paraffin wax, and sliced into 4-µm-thick sections.

Article Title: FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo
Article Snippet: .. Samples were blocked for non-specific protein binding with 5% normal goat serum in PBS-T for 1 hour and incubated with anti-bFIZ1 antibody (1:100, 4°C), a rabbit IgG made to target domain-II-IV of FIZ1 (Proteintech Group Inc., Chicago, IL, USA) that was affinity purified and specific for FIZ1 in immunoblots of mammalian retina [ , ]. .. After washing with PBS-T (3 × 5 min), samples were incubated with Nanogold-Fab'goat anti-rabbit-IgG nano gold (gold particle size 1.5 nm maximum, 1.4 nm average), 1:50 in PBS-T, 1 hour at 37°C (Nanoprobes, Yaphank, NY).

Article Title: Leptocarpin Suppresses Proliferation, Migration, and Invasion of Human Osteosarcoma by Targeting Type-1 Insulin-Like Growth Factor Receptor (IGF-1R)
Article Snippet: .. Then, the protein was transferred to polyvinylidene fluoride (PVDF) membranes (Merck Millipore Corporation, Darmstadt, Germany), and the membranes were blocked with 5% non-fat milk and 2% bovine serum albumin (BSA, Gen-View Scientific Inc., USA) in PBS-T (PBS containing 0.25% Tween-20, Macklin) at 25°C for 1.5 h. The membranes were incubated with rabbit anti-IGF-1R primary antibodies (1: 4000, Proteintech Group Inc., Wuhan, China) and mouse anti-β-actin (1: 10 000, Proteintech), as internal control, at 4°C overnight. .. The next day, the membranes were incubated with goat anti-rabbit and goat anti-mouse secondary antibodies (1: 3000, Jackson ImmunoResearch Laboratories, Inc. USA) at 25°C for 2 h, and then analyzed with electrochemiluminescence (ECL, Millipore, MA, USA).

Article Title: Molecular Characterization of Severin from Clonorchis sinensis Excretory/Secretory Products and Its Potential Anti-apoptotic Role in Hepatocarcinoma PLC Cells
Article Snippet: .. Successively, the membranes were blocked with 1% bovine serum albumin in phosphate-buffered saline (PBS) overnight at 4°C, washed five times with PBS-0.05% Tween 20 (PBS-T, pH 7.4), and incubated with His-tag monoclonal antibody, sera from naïve rats, rCs severin immunized rats, C. sinensis -infected rats or Cs ESPs immunized rats (1∶100 dilutions) followed by HRP-conjugated goat anti-mouse/rat IgG (Proteintech; dilution of 1∶2,000) at 37°C for 2 h. After adequately washing with PBS-T, the membrane was incubated with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG in 1∶2000 dilutions (Proteintech, USA) at 37°C for 1 h. Detection was then carried out by enhanced chemiluminescence (ECL) method. .. Expression level of Cs severin at life-stages of C. sinensis Intact living adult worms were collected from biliary tracts of infected cats and washed extensively and gently in physiological saline to remove any contamination from hosts.

Article Title: Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres
Article Snippet: .. Chambers were washed in PBS-T (PBS with 0.1% Triton X-100) and blocked in 3% BSA for 1 h at RT. mDia2 and mDia1 (1:100) or Connexin-43 (1:200) (Proteintech, Rosemont, IL, USA), β-Tubulin (1:100) (Sigma), Glu-Tubulin (1:100) (Millipore Sigma, Burlington, VT, USA) antibodies were incubated at 4 °C for 48–72 h. Invasions were washed with PBS-T before adding AlexaFluor 2° antibodies (1:200–500), AlexaFluor Phalloidin (1:100), or DAPI (1:50) (Molecular Probes, Eugene, OR, USA) for 24–48 h at 4 °C. .. The wells were imaged on a Leica TCS SP5 multiphoton laser-scanning confocal microscope.

Article Title: Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis
Article Snippet: .. 5% skim milk diluted in PBS-T (1% of Tween-20 in PBS) was used to block the membrane at room temperature for 1 h. Primary antibody diluted with PBS-T was incubated with the membrane at 4 °C overnight (TRAIL, ProteinTech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Abcam) antibody with a dilution of 1:1000). .. After the membrane was washed with PBS-T three times for 5 min, the membrane was then incubated with horseradish peroxidase-conjugated secondary antibody (CST) diluted with PBS-T for 1 h at room temperature.

Article Title: Hyperbaric oxygen treatment mitigates liver damage in mice with noise exposure.
Article Snippet: .. Noise exposure relates to various pathological disorders including liver damage, preventive measures of which are being demanded. .. Noise exposure relates to various pathological disorders including liver damage, preventive measures of which are being demanded.

Blocking Assay:

Article Title: Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis
Article Snippet: .. 5% skim milk diluted in PBS-T (1% of Tween-20 in PBS) was used to block the membrane at room temperature for 1 h. Primary antibody diluted with PBS-T was incubated with the membrane at 4 °C overnight (TRAIL, ProteinTech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Abcam) antibody with a dilution of 1:1000). .. After the membrane was washed with PBS-T three times for 5 min, the membrane was then incubated with horseradish peroxidase-conjugated secondary antibody (CST) diluted with PBS-T for 1 h at room temperature.

Western Blot:

Article Title: FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo
Article Snippet: .. Samples were blocked for non-specific protein binding with 5% normal goat serum in PBS-T for 1 hour and incubated with anti-bFIZ1 antibody (1:100, 4°C), a rabbit IgG made to target domain-II-IV of FIZ1 (Proteintech Group Inc., Chicago, IL, USA) that was affinity purified and specific for FIZ1 in immunoblots of mammalian retina [ , ]. .. After washing with PBS-T (3 × 5 min), samples were incubated with Nanogold-Fab'goat anti-rabbit-IgG nano gold (gold particle size 1.5 nm maximum, 1.4 nm average), 1:50 in PBS-T, 1 hour at 37°C (Nanoprobes, Yaphank, NY).

Affinity Purification:

Article Title: FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo
Article Snippet: .. Samples were blocked for non-specific protein binding with 5% normal goat serum in PBS-T for 1 hour and incubated with anti-bFIZ1 antibody (1:100, 4°C), a rabbit IgG made to target domain-II-IV of FIZ1 (Proteintech Group Inc., Chicago, IL, USA) that was affinity purified and specific for FIZ1 in immunoblots of mammalian retina [ , ]. .. After washing with PBS-T (3 × 5 min), samples were incubated with Nanogold-Fab'goat anti-rabbit-IgG nano gold (gold particle size 1.5 nm maximum, 1.4 nm average), 1:50 in PBS-T, 1 hour at 37°C (Nanoprobes, Yaphank, NY).

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