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Pharmingen pbs t
Pbs T, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Pharmingen
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-09
92/100 stars

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Incubation:

Article Title: Attenuated Modified Vaccinia Virus Ankara Can Be Used as an Immunizing Agent under Conditions of Preexisting Immunity to the Vector
Article Snippet: .. Plates were incubated in a humidified atmosphere (37°C, 24 h), washed extensively with PBS-T, and incubated (room temperature, 2 h) with a solution in PBS-T of biotinylated anti-mouse IFN-γ monoclonal antibody XMG1.2 (2 μg/ml) (PharMingen). .. The plates were washed with PBS-T, and 100 μl of peroxidase-labeled avidin (1/800 dilution in PBS-T) (Sigma) was added to each well and incubated at room temperature for 1 h. After washing, spots were developed by adding the substrate 3,3′-diaminobenzidine tetrahydrochloride (1 μg/ml) (Sigma) in 50 mM Tris-HCl (pH 7.5) containing 0.015% hydrogen peroxide and counted using an MZ122 APO and Imaging System QWIN software (Leica, Cambridge, United Kingdom).

Article Title: Intranodal Immunization With a Vaccinia Virus Encoding Multiple Antigenic Epitopes and Costimulatory Molecules in Metastatic Melanoma
Article Snippet: .. After three washes with PBS-T, 100 µl of a 1/1,000 antihuman-Ig-biotinylated antibody preparation (Pharmingen, SanDiego, CA) diluted in 3% BSA were added for a 4 5 ̀ incubation. ..

Article Title: Extended JAK Activation and Delayed STAT1 Dephosphorylation Contribute to the Distinct Signaling Profile of CNS Neurons Exposed to Interferon-Gamma
Article Snippet: .. For analysis of STAT1 phosphorylation, the blots were incubated for 1 hr at room temperature in primary antibody (anti-STAT1p (1:1000); BD Biosciences Pharmingen) diluted in PBS-T containing 3% BSA. .. After three washes in PBS-T (5 min each), the blots were incubated in secondary antibody solution (goat anti-mouse horseradish peroxidase (HRP); 1:2000; Santa Cruz Biotechnology Inc.) for 1 hr at room temperature, and visualized as described above.

Article Title: Biology of Attenuated Modified Vaccinia Virus Ankara Recombinant Vector in Mice: Virus Fate and Activation of B- and T-Cell Immune Responses in Comparison with the Western Reserve Strain and Advantages as a Vaccine
Article Snippet: .. Plates were incubated for 24 h in a 37°C incubator with a 5% CO2 atmosphere, washed extensively with PBS-T, and incubated for 2 h at 37°C with a solution of 2 μg of biotinylated anti-mouse IFN-γ monoclonal antibody XMG1.2 (PharMingen) per ml in PBS-T. .. Thereafter, plates were washed with PBS-T, 100 μl of peroxidase-labeled avidin (Sigma) at a 1/800 dilution in PBS-T was added to each well, and the plates were incubated at room temperature for 1 h. Wells were washed with PBS-T and PBS, and the spots were developed by adding 1 μg of the substrate DAB (Sigma) in 50 mM Tris-HCl (pH 7.5) containing 0.015% hydrogen peroxide.

Article Title: Prime-Boost Immunization Schedules Based on Influenza Virus and Vaccinia Virus Vectors Potentiate Cellular Immune Responses against Human Immunodeficiency Virus Env Protein Systemically and in the Genitorectal Draining Lymph Nodes
Article Snippet: .. Plates were incubated for 24 h in a 37°C incubator with a 5% CO2 atmosphere, washed extensively with PBS-T, and incubated for 2 h with a solution of 2 μg of biotinylated anti-mouse IFN-γ monoclonal antibody XMG1.2 (PharMingen) per ml in PBS-T. .. Thereafter, plates were washed with PBS-T, and 100 μl of peroxidase-labeled avidin (Sigma Chemical) at a 1:800 dilution in PBS-T was added to each well and incubated at room temperature.

other:

Article Title: Neuropeptides, by direct interaction with T cells, induce cytokine secretion and break the commitment to a distinct T helper phenotype
Article Snippet: Supernatants were then discarded, the plates washed (four times for 10 min) with PBS-T (PBS/0.05% Tween), and then exposed to 100 μl of 2 mg/ml (in 10% FCS/PBS) of biotin-conjugated anti-murine cytokine antibody (PharMingen) (overnight, 4°C).

Avidin-Biotin Assay:

Article Title: High Quality Long-Term CD4+ and CD8+ Effector Memory Populations Stimulated by DNA-LACK/MVA-LACK Regimen in Leishmania major BALB/c Model of Infection
Article Snippet: .. Afterwards, plates were washed with PBS-T and 100 µl of peroxidase-labeled avidin (PharMingen, San Diego, CA) at a 1/800 dilution in PBS-T was added to each well. .. After 1 h of incubation at room temperature, wells were washed with PBS-T and PBS.

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  • 92
    Pharmingen tween 20 pbs t
    Tween 20 Pbs T, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbs t/product/Pharmingen
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbs t - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Pharmingen pbs t
    Pbs T, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Pharmingen
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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