Structured Review

Merck & Co pbs t
Pbs T, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Merck & Co
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Western Blot:

Article Title: Downregulation of class II phosphoinositide 3-kinase PI3K-C2β delays cell division and potentiates the effect of docetaxel on cancer cell growth
Article Snippet: .. After washing with PBS-T, membranes were incubated with secondary antibodies (Sigma Aldrich, peroxidase conjugate goat anti-rabbit IgG, cat number: A6154, peroxidase conjugate goat anti-mouse IgG, cat number: A0168, 1:10,000) for 1 h at room temperature, washed with PBS-T and exposed to ECL reagent (Merck™ Immobilon™ Western Chemiluminescent HRP Substrate, cat number: 11546345, Thermo Fisher Scientific). .. Immunofluorescence analysis For immunofluorescence analysis, cells were seeded onto coverslips in 12 well plates.

Article Title: Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
Article Snippet: .. Probed membranes were washed three times in PBS-T and after incubation with Immobilon Western Chemiluminescent HRP Substrate (Merck), imaged with a ChemiDoc Touch Imaging System (Bio-Rad Laboratories). .. Transmission electron microscopy TEM was performed as previously described [ ].

Next-Generation Sequencing:

Article Title: Distinct Functions of Syntaxin-1 in Neuronal Maintenance, Synaptic Vesicle Docking, and Fusion in Mouse Neurons
Article Snippet: .. Both cultures were fixed in 4% PFA for 20 min, permeabilized with 1× PBS containing 0.1% Tween 20 (PBS-T) for 20 min, and blocked with PBS-T containing 2.5% normal goat serum (NGS) and 2.5% normal donkey serum (NDS) for 1 h. The cortical cultures fixed at DIV 2 and 4 were incubated overnight at 4°C with chicken polyclonal anti-MAP2 (Merck) primary antibody (1:2000) in PBS-T containing NGS and NDS. .. The cortical cultures fixed at DIV 22 and the hippocampal cultures were subjected to guinea pig polyclonal anti-Bassoon (Synaptic Systems) primary antibody (1:2500), which was used as a synaptic marker, in addition to the primary antibodies against Stx1A (1:1000) and Stx1B (1:1000) mentioned above, overnight at 4°C.

Incubation:

Article Title: Distinct Functions of Syntaxin-1 in Neuronal Maintenance, Synaptic Vesicle Docking, and Fusion in Mouse Neurons
Article Snippet: .. Both cultures were fixed in 4% PFA for 20 min, permeabilized with 1× PBS containing 0.1% Tween 20 (PBS-T) for 20 min, and blocked with PBS-T containing 2.5% normal goat serum (NGS) and 2.5% normal donkey serum (NDS) for 1 h. The cortical cultures fixed at DIV 2 and 4 were incubated overnight at 4°C with chicken polyclonal anti-MAP2 (Merck) primary antibody (1:2000) in PBS-T containing NGS and NDS. .. The cortical cultures fixed at DIV 22 and the hippocampal cultures were subjected to guinea pig polyclonal anti-Bassoon (Synaptic Systems) primary antibody (1:2500), which was used as a synaptic marker, in addition to the primary antibodies against Stx1A (1:1000) and Stx1B (1:1000) mentioned above, overnight at 4°C.

Article Title: Downregulation of class II phosphoinositide 3-kinase PI3K-C2β delays cell division and potentiates the effect of docetaxel on cancer cell growth
Article Snippet: .. After washing with PBS-T, membranes were incubated with secondary antibodies (Sigma Aldrich, peroxidase conjugate goat anti-rabbit IgG, cat number: A6154, peroxidase conjugate goat anti-mouse IgG, cat number: A0168, 1:10,000) for 1 h at room temperature, washed with PBS-T and exposed to ECL reagent (Merck™ Immobilon™ Western Chemiluminescent HRP Substrate, cat number: 11546345, Thermo Fisher Scientific). .. Immunofluorescence analysis For immunofluorescence analysis, cells were seeded onto coverslips in 12 well plates.

Article Title: Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
Article Snippet: .. Probed membranes were washed three times in PBS-T and after incubation with Immobilon Western Chemiluminescent HRP Substrate (Merck), imaged with a ChemiDoc Touch Imaging System (Bio-Rad Laboratories). .. Transmission electron microscopy TEM was performed as previously described [ ].

Imaging:

Article Title: Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
Article Snippet: .. Probed membranes were washed three times in PBS-T and after incubation with Immobilon Western Chemiluminescent HRP Substrate (Merck), imaged with a ChemiDoc Touch Imaging System (Bio-Rad Laboratories). .. Transmission electron microscopy TEM was performed as previously described [ ].

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  • 92
    Merck & Co pbs t buffer
    Pbs T Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t buffer/product/Merck & Co
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbs t buffer - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Merck & Co pbs t
    Pbs T, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Merck & Co
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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