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Jackson Immuno pbs t
Pbs T, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Jackson Immuno
Average 94 stars, based on 764 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
94/100 stars

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Incubation:

Article Title: Moxibustion-Simulating Bipolar Radiofrequency Suppresses Weight Gain and Induces Adipose Tissue Browning via Activation of UCP1 and FGF21 in a Mouse Model of Diet-Induced Obesity
Article Snippet: .. After five washes in PBS-T, whole-mounted tissues were incubated for four hours at room temperature with Cy3-conjugated anti-rat antibody (diluted 1:500; Jackson ImmunoResearch Laboratories Inc.) in blocking solution. .. For negative control experiments, primary or secondary antibodies were omitted during the immunohistochemistry process.

Article Title: Impact of pre-existing dengue immunity on human antibody and memory B cell responses to Zika
Article Snippet: .. After three washes with PBS-T, wells were blocked with human gamma globulin (Jackson ImmunoResearch) diluted 1:500 in 1× DPBS, followed by incubation with Qdot-labeled mAbs diluted 1:1000 in DPBS 1× for 1 h at 37 °C. .. Finally, plates were washed 3 times with PBS-T and once with ddH2 O and dried using a 96-well MultiScreen Vacuum Manifold (Millipore).

Article Title: RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity
Article Snippet: .. Immunological Methods Cells were washed (PBS), fixed (PBS, 3% paraformaldehyde; 15 min), washed (PBS), permeabilized in PBS-T (PBS plus 0.3% Triton X-100; 20 min), blocked (PBS-T plus 5% goat serum; 15 min) and incubated with primary antibodies overnight at 4°C and appropriate secondaries antibodies (Jackson ImmunoResearch, dilution 1:200; 1 h, room temperature). .. The primary antibodies used were: rabbit anti-RanBP1 (1:500, SAB1411184, Sigma-Aldrich), rabbit polyclonal anti-RanBP1 (#8780, Cell Signaling), rabbit polyclonal anti-RanBP1 (1:4000, ab97659 Abcam), chicken anti-Tau (1:1000, ab75714 Abcam), mouse anti-Tau (1:1000, mab3420, Millipore), mouse anti-TGN38 (1:100, NB300-575SS, Novusbio), mouse anti-MAP2 (1:1000, ab11267, Abcam), rabbit anti-MAP2 (1:1000, ab5622 Millipore), mouse anti-LKB1 (1:100, NBP2-14835SS, Novusbio), rabbit anti-Ran (GTP+GDP) (1:1000, SAB4502579, Sigma-Aldrich), mouse anti-RanGAP1 (1:200, sc-28322, Santa Cruz Biotechnology), mouse anti-Myc (1:500, 9E10, Santa Cruz), chicken anti-GFP (1:1000, ab13970, Abcam) and chicken anti-Tuj1 (1:1000, ab107216, Abcam).

Article Title: Anatomy and development of the larval nervous system in Echinococcus multilocularis
Article Snippet: .. Then, samples were washed five times for 30 min with PBS-T, and incubated overnight with the FITC conjugated secondary antibody (donkey anti-mouse or donkey anti-rabbit as required, Jackson Immunoresearch) at 4°C. .. Finally the samples were washed five times for 30 min with PBS-T, and co-stained with 4',6-diamidino-2-phenylindole (DAPI) and TRITC-conjugated Phalloidin (Sigma-Aldrich) as previously described [ ].

Article Title: A peroxisome proliferator-activated receptor-? agonist provides neuroprotection in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease
Article Snippet: .. Cells were first incubated in primary antibodies [mouse tyrosine hydroxylase (1:200; Millipore, Watford, UK), PPARδ (1:100), Chemicon, Temecula, CA)] overnight at 4 °C before incubation in 1% normal goat serum in PBS-T with secondary antibodies [goat anti-mouse Cy3 (1:200; Jackson ImmunoResearch, West Grove, PA)] for 1 h at room temperature. .. The coverslips were mounted, sealed and imaged by fluorescent microscopy at the same setting (LSM700, Carl Zeiss, Hertfordshire, UK).

Article Title: Parallel Induction of CH505 B Cell Ontogeny-Guided Neutralizing Antibodies and tHIVconsvX Conserved Mosaic-Specific T Cells against HIV-1
Article Snippet: .. After washing 3× with PBS-T, the CH505w100 trimer at 0.2 μg/mL in SuperBlock was added to all wells at room temperature (RT) for 2 h. After washing 3× in PBS-T, serial 5-fold dilutions of sera in SuperBlock starting from 1:100 were added to wells at RT for 2 h; the plates were washed 5× in PBS-T, incubated with 50 μL/well of secondary anti-mouse IgG HRP-conjugated antibody at 0.05 μg/mL (715-035-150, Jackson ImmunoResearch Laboratories) in Superblock at RT for 1 h, and washed 3× with PBS-T. Alternatively, for IgG isotype determination, biotinylated secondary anti-mouse IgG1, IgG2a, IgG2b, and IgG3 mAbs were used (BD Biosciences 553441, 553388, 553393, and 553401, respectively), diluted to 1 μg/mL in PBS-T. ..

Article Title: Nidogen Is Nonessential and Not Required for Normal Type IV Collagen Localization in Caenorhabditis elegans
Article Snippet: .. Samples were washed three times with PBS-T, incubated for 1 h at room temperature with FITC-labeled donkey anti-rabbit and Texas red-labeled donkey anti-mouse secondary antibodies ( Jackson ImmunoResearch , West Grove, PA) in PBS-T-NDS, and washed with PBS-T and PBS. ..

Blocking Assay:

Article Title: Moxibustion-Simulating Bipolar Radiofrequency Suppresses Weight Gain and Induces Adipose Tissue Browning via Activation of UCP1 and FGF21 in a Mouse Model of Diet-Induced Obesity
Article Snippet: .. After five washes in PBS-T, whole-mounted tissues were incubated for four hours at room temperature with Cy3-conjugated anti-rat antibody (diluted 1:500; Jackson ImmunoResearch Laboratories Inc.) in blocking solution. .. For negative control experiments, primary or secondary antibodies were omitted during the immunohistochemistry process.

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    Jackson Immuno pbs t
    Pbs T, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Jackson Immuno
    Average 94 stars, based on 764 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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