pbs t (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc pbs t
Pbs T, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Cell Signaling Technology Inc
Average 94 stars, based on 405 article reviews
Price from $9.99 to $1999.99

pbs t - by Bioz Stars, 2020-07

94/100 stars

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Western Blot:

Article Title: High-mobility group box 1 and the receptor for advanced glycation end products contribute to lung injury during Staphylococcus aureus pneumonia
Article Snippet: .. After washing with PBS-T, membranes were probed with anti-rabbit-HRP-conjugated secondary antibody (Cell Signaling Technology, Danvers, MA, USA) for 1 hour at room temperature in 1% bovine serum albumin in PBS-T. After washing with PBS-T, membranes were incubated with Lumi-Light Plus Western Blotting Substrate (Roche, Mijdrecht, The Netherlands) and positive bands were detected using an LAS3000 Luminescent image Analyzer dark box (Fujifilm, Tokyo, Japan). .. Band densities were determined using AIDA Image analyzer software (Raytest, Straubenhardt, Germany) and expressed as a percentage of band densities of naive control samples.

Incubation:

Article Title: Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis
Article Snippet: .. Membranes were blocked in 5% milk for 1 h at room temperature and incubated overnight rocking at 4°C in 5% milk PBS ‐ T. Membranes were washed in PBS ‐ T and incubated for 1 h with peroxidase ‐ conjugated secondary antibody (Cell Signalling, Hitchin, UK). .. Clarity Western blotting ECL substrate (Bio‐Rad) was used to generate a signal that was detected using a Chemidoc imaging system (Bio‐Rad).

Article Title: Combined cisplatin and aurora inhibitor treatment increase neuroblastoma cell death but surviving cells overproduce BDNF
Article Snippet: .. The upper part of the membrane was blocked with fat-free 5% milk in PBS-T, then incubated overnight at 4°C alternatively with the following antibodies: anti-phospho Aurora kinases (1:1000, polyclonal; 2914S, Cell Signaling Technology, MA, USA), anti-aurora kinase A (1:1000, polyclonal; 3092S, Cell Signaling Technology), or with anti-αTubulin antibody (1:8000, monoclonal; T6074, Sigma-Aldrich). .. After three washing steps, a further incubation for 1 h at room temperature was performed with secondary HRP-conjugated antibodies, specifically anti-rabbit against primary polyclonal antibodies (1:10,000 in fat-free 5% milk in PBS-T), or anti-mouse for monoclonal antibody (1:10,000 in fat-free 5% milk in PBS-T; RABHRP1, Sigma-Aldrich).

Article Title: Expression of mitochondrial branched-chain aminotransferase and ?-keto-acid dehydrogenase in rat brain: implications for neurotransmitter metabolism
Article Snippet: .. Following this, the membrane was washed three times (5 min each) with PBS-T, and then incubated with anti-rabbit secondary antibodies (Cell Signaling, Danvers, MA) for 1 h. Enhanced chemiluminescence detection (Millipore, Billerica, MA) was used to detect the bands. .. BCATm in the cerebellum and hippocampal formation In the cerebellum, immunolabeling for BCATm was most prominent in astrocytes of the white matter tracts and in the distal processes of the Bergmann glia of the cerbellar cortex (Figure ).

Article Title: MicroRNA-378 Regulates Adiponectin Expression in Adipose Tissue: A New Plausible Mechanism
Article Snippet: .. Membranes were washed 3X with PBS-T and incubated with HRP-conjugated anti-rabbit IgG (#7074, Cell Signaling Technologies) or HRP-conjugated anti-mouse IgG (BD Biosciences) in 5% skimmed milk in PBS-T at room temperature for 1hr. .. After washes 5 X with PBS-T, the bands were visualized by enhanced chemiluminescence (ECL, Perkin-Elmer) and quantified using by a CCD camera system (LAS4000mini, GE Healthcare) with ImageQuant TL software (GE Healthcare).

Labeling:

Article Title: Iberis amara Extract Induces Intracellular Formation of Reactive Oxygen Species and Inhibits Colon Cancer
Article Snippet: .. Cells were washed with PBS-T (0.3%) and labeled by anti-rabbit IgG (H+L), F(ab')2 fragment Alexa Fluor 488 Conjugate (#4409, Cell Signaling Technology) diluted (1:1,000) in 1% BSA/PBS-T (0.3%) for 1 h at room temperature. .. F-actin was stained with Texas Red-X phalloidin (Life Technologies) for 30 min at room temperature.

pbs t (Cell Signaling Technology Inc)

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