Structured Review

Atlas Antibodies pbs t
Pbs T, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Atlas Antibodies
Average 91 stars, based on 7 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
91/100 stars

Images

Related Articles

Western Blot:

Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy
Article Snippet: .. Unless otherwise specified, all sera were diluted 1:100 in 0.5% dry milk in PBS-T. For specific detection of the PLA2 R1 and THSD7A antigens in the positive control of Western blot analyses the following commercially available antibodies were used: rabbit polyclonal antibody at a 1:1000 dilution in 0.5% dry milk in PBS-T (Atlas Antibodies) for PLA2 R1; rabbit polyclonal antibody at a 1:1,000 dilution in 0.5% dry milk in PBS-T (Atlas Antibodies) for THSD7A. .. A horseradish peroxidase–conjugated goat anti-rabbit IgG (Sigma-Aldrich, #A9169) was used as secondary antibody at a 1:20,000 dilution.

Incubation:

Article Title: Mechanism and regulation of the Lys6-selective deubiquitinase USP30
Article Snippet: .. Membranes were blocked in a 5% (w/v) milk solution in PBS-T (PBS + 0.1% (v/v) Tween-20) for 30 min and incubated overnight at 4 °C with a primary antibody recognizing either USP30 (HPA016952, Atlas Antibodies), ubiquitin (Ubi-1, NB300-130, Novus Biologicals) or pSer65 ubiquitin (ABS1513-I, Millipore). .. The membrane was then washed with PBS-T, incubated for 1 h at room temperature with anti-rabbit or anti-mouse IgG-HRP (NA934V or NXA931, GE Healthcare) in 5% (w/v) milk in PBS-T, washed in PBS-T, visualised with Amersham Western Blotting Detection Reagent (GE Healthcare) and imaged using a ChemiDocTM Touch Imaging System (Bio-Rad).

Positive Control:

Article Title: Antigen-Specific IgG Subclasses in Primary and Malignancy-Associated Membranous Nephropathy
Article Snippet: .. Unless otherwise specified, all sera were diluted 1:100 in 0.5% dry milk in PBS-T. For specific detection of the PLA2 R1 and THSD7A antigens in the positive control of Western blot analyses the following commercially available antibodies were used: rabbit polyclonal antibody at a 1:1000 dilution in 0.5% dry milk in PBS-T (Atlas Antibodies) for PLA2 R1; rabbit polyclonal antibody at a 1:1,000 dilution in 0.5% dry milk in PBS-T (Atlas Antibodies) for THSD7A. .. A horseradish peroxidase–conjugated goat anti-rabbit IgG (Sigma-Aldrich, #A9169) was used as secondary antibody at a 1:20,000 dilution.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Atlas Antibodies pbs t
    Pbs T, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Atlas Antibodies
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results