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Agilent technologies pbs t
Antigen screening using multi-antigen print immunoassay. Staining of antigen printed onto nitrocellulose by sera from 5 negative controls (Danish participants, no known TB exposure of TB risk factors) and 19 patients with confirmed TB, recruited from Danish hospitals. The nitrocellulose strips were incubated with sera diluted 1/50 in 1% non-fat skim milk in <t>PBS-T.</t>
Pbs T, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Agilent technologies
Average 94 stars, based on 516 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
94/100 stars

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1) Product Images from "Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis"

Article Title: Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis

Journal: BMC Research Notes

doi: 10.1186/1756-0500-6-202

Antigen screening using multi-antigen print immunoassay. Staining of antigen printed onto nitrocellulose by sera from 5 negative controls (Danish participants, no known TB exposure of TB risk factors) and 19 patients with confirmed TB, recruited from Danish hospitals. The nitrocellulose strips were incubated with sera diluted 1/50 in 1% non-fat skim milk in PBS-T.
Figure Legend Snippet: Antigen screening using multi-antigen print immunoassay. Staining of antigen printed onto nitrocellulose by sera from 5 negative controls (Danish participants, no known TB exposure of TB risk factors) and 19 patients with confirmed TB, recruited from Danish hospitals. The nitrocellulose strips were incubated with sera diluted 1/50 in 1% non-fat skim milk in PBS-T.

Techniques Used: Staining, Incubation

2) Product Images from "Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis"

Article Title: Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis

Journal: BMC Research Notes

doi: 10.1186/1756-0500-6-202

Antigen screening using multi-antigen print immunoassay. Staining of antigen printed onto nitrocellulose by sera from 5 negative controls (Danish participants, no known TB exposure of TB risk factors) and 19 patients with confirmed TB, recruited from Danish hospitals. The nitrocellulose strips were incubated with sera diluted 1/50 in 1% non-fat skim milk in PBS-T.
Figure Legend Snippet: Antigen screening using multi-antigen print immunoassay. Staining of antigen printed onto nitrocellulose by sera from 5 negative controls (Danish participants, no known TB exposure of TB risk factors) and 19 patients with confirmed TB, recruited from Danish hospitals. The nitrocellulose strips were incubated with sera diluted 1/50 in 1% non-fat skim milk in PBS-T.

Techniques Used: Staining, Incubation

3) Product Images from "Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β"

Article Title: Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β

Journal: Scientific Reports

doi: 10.1038/s41598-017-06377-8

Binding profiling of five Affibody molecules to different Aβ aggregates, analyzed by ELISA. ( a ) Aβ 42 wt and Aβ 42 cc aggregates (50 nM assay concentration), bound to Affibody molecules, were detected by 6E10-HRP. 6E10 21 recognizes the N-terminus of Aβ. As expected, all five Affibody molecules show binding to Aβ 42 cc protofibrils. The Affibody molecule Z Aβ42cc_1 also binds to Aβ 42 wt protofibrils. No binding could be observed to either wild type monomer or fibrils. ( b ) Same as in ( a ), except that the assay concentration of Aβ 42 was 1 µM and mAb1C3 was used for detection (weakly specific for protofibrils 21 ). The binding profile has the same pattern as in ( a ) for protofibrils (both wt and cc) and Aβ 42 wt fibrils, but with a higher background for Aβ 42 wt protofibrils. ( a , b ) Two controls involve replacing the Affibody molecule with either an irrelevant Affibody molecule (Z Taq_2 ) or by PBS-T. In a third control experiment PBS-T was added instead of Aβ-specific antibody (6E10 or mAb1C3). Values are means of duplicate experiments.
Figure Legend Snippet: Binding profiling of five Affibody molecules to different Aβ aggregates, analyzed by ELISA. ( a ) Aβ 42 wt and Aβ 42 cc aggregates (50 nM assay concentration), bound to Affibody molecules, were detected by 6E10-HRP. 6E10 21 recognizes the N-terminus of Aβ. As expected, all five Affibody molecules show binding to Aβ 42 cc protofibrils. The Affibody molecule Z Aβ42cc_1 also binds to Aβ 42 wt protofibrils. No binding could be observed to either wild type monomer or fibrils. ( b ) Same as in ( a ), except that the assay concentration of Aβ 42 was 1 µM and mAb1C3 was used for detection (weakly specific for protofibrils 21 ). The binding profile has the same pattern as in ( a ) for protofibrils (both wt and cc) and Aβ 42 wt fibrils, but with a higher background for Aβ 42 wt protofibrils. ( a , b ) Two controls involve replacing the Affibody molecule with either an irrelevant Affibody molecule (Z Taq_2 ) or by PBS-T. In a third control experiment PBS-T was added instead of Aβ-specific antibody (6E10 or mAb1C3). Values are means of duplicate experiments.

Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

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Plasmid Preparation:

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Article Snippet: .. The slides were then placed on a DAKO autostainer with the following program; 1) one PBS/T wash; 2) incubation with a biotinylated secondary antibody [Vector Elite kit] for 30 min.; 3) one PBS/T wash; and then 4) incubation with ABC reagent [Vector Elite kit] for 30 min. PBS/T was used as a final wash and chromogen-DAB [DAKO] was applied for 5 min. (color reaction product – brown). .. Image analysis Tumor microarrays were examined by light microscopy and cores were categorized into either positive (+) or negative (-) staining for TACC3 and TACC1, with (+) staining representing those cores where greater than 10% of the cells had detectable staining, otherwise they were scored (-).

Incubation:

Article Title: Development of a proof of concept immunochromatographic lateral flow assay for point of care diagnosis of Mycobacterium tuberculosis
Article Snippet: .. The plates were then washed with PBS-T, and then incubated for 1 hour with rabbit anti-human immunoglobulin G antibody conjugated with horseradish peroxidase (D0336; Dako, Hillerød, Denmark) diluted 1:1,000 in PBS-T. .. The plates were washed with PBS-T and enzyme activity was assayed by incubation for 30 min at room temperature with 100 μl of tetramethylbenzidine peroxidase substrate (Bio-Rad, Hercules, Calif.) per well.

Article Title: Functional characterization of two enhancers located downstream FOXP2
Article Snippet: .. After several PBS-T washes the membranes were incubated for 1 h at room temperature with secondary antibodies horseradish peroxidase (HRP)-conjugated with goat anti-mouse (1/1000) and goat anti-Rabbit (1/500; Dako, Barcelona, Spain) diluted in PBS-T. After several PBS-T washes the membranes were developed with enhanced chemiluminiscence (ECL) (GE Healthcare). ..

Article Title: Aberrations of TACC1 and TACC3 are associated with ovarian cancer
Article Snippet: .. The slides were then placed on a DAKO autostainer with the following program; 1) one PBS/T wash; 2) incubation with a biotinylated secondary antibody [Vector Elite kit] for 30 min.; 3) one PBS/T wash; and then 4) incubation with ABC reagent [Vector Elite kit] for 30 min. PBS/T was used as a final wash and chromogen-DAB [DAKO] was applied for 5 min. (color reaction product – brown). .. Image analysis Tumor microarrays were examined by light microscopy and cores were categorized into either positive (+) or negative (-) staining for TACC3 and TACC1, with (+) staining representing those cores where greater than 10% of the cells had detectable staining, otherwise they were scored (-).

Article Title: Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins. Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins
Article Snippet: .. In order to block nonspecific labeling, cells were incubated with PBS‐T containing 10% normal goat serum (Dako, Baar, Switzerland) for 30 min and then incubated with rabbit Listeria O antiserum (BD, Allschwil, Switzerland, 1:200) in PBS‐T with 10% of goat serum for 1 hr at RT. .. Coverslips were then washed three times in PBS‐T and then incubated with Alexa Fluor 488‐ conjugated goat anti‐rabbit IgG secondary antibody (Life technologies, 1:500) and with DAPI (Invitrogen, Carlsbad, CA, USA, T3604, 1:10,000) for one hour in the dark.

Article Title: Development of ELISA based detection system for lethal toxin of Clostridium sordellii
Article Snippet: .. The membrane was again washed three times with PBS-T and once with PBS and then the membrane was incubated in 1:2000 dilution of rabbit anti-mouse IgG horseradish peroxidase (HRP) (Dako, Denmark) as secondary antibody with gentle shaking at room temperature for 1.0 h. The membrane was washed and the protein bands were visualized by incubating the membrane in 3,3’-diaminobenzidine in PBS containing 8.8mM H2 O2 . ..

Article Title: Genetic determinants of anti-malarial acquired immunity in a large multi-centre study
Article Snippet: .. After overnight incubation at 4 °C, plates were washed six times with PBS/T, 50 µl of horseradish peroxidise-conjugated rabbit anti-human IgG (DAKO) (1:5,000 in PBS/T) added to each well and plates incubated for 3 h at room temperature. .. Following six-fold washing in PBS/T, 100 µl of Sigma-Fast o-phenylenediamine dihydrochloride (OPD) reagent solution (Sigma) was added to each well.

Article Title: Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β
Article Snippet: .. The plates were washed four times with PBS-T, prior to addition of 42 nM biotinylated Aβ42 cc protofibrils per well and incubated for 1 h. After washing the wells four times, streptavidin-HRP (Dako) diluted 1:30,000 in PBSC was added to the wells and incubated for 1 h. TMB substrates A and B were mixed 1:1 and added to washed wells and incubated for 7 min according to the manufacturer’s instructions (ImmunoPure TMB Substrate Kit; Thermo Scientific). .. Stop solution (2 M H2 SO4 ) was added and the absorbance at 450 nm was measured in an ELISA reader (Victor 3, Perkin Elmer).

Blocking Assay:

Article Title: Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins. Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins
Article Snippet: .. In order to block nonspecific labeling, cells were incubated with PBS‐T containing 10% normal goat serum (Dako, Baar, Switzerland) for 30 min and then incubated with rabbit Listeria O antiserum (BD, Allschwil, Switzerland, 1:200) in PBS‐T with 10% of goat serum for 1 hr at RT. .. Coverslips were then washed three times in PBS‐T and then incubated with Alexa Fluor 488‐ conjugated goat anti‐rabbit IgG secondary antibody (Life technologies, 1:500) and with DAPI (Invitrogen, Carlsbad, CA, USA, T3604, 1:10,000) for one hour in the dark.

Labeling:

Article Title: Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins. Hyperinvasiveness of Listeria monocytogenes sequence type 1 is independent of lineage I‐specific genes encoding internalin‐like proteins
Article Snippet: .. In order to block nonspecific labeling, cells were incubated with PBS‐T containing 10% normal goat serum (Dako, Baar, Switzerland) for 30 min and then incubated with rabbit Listeria O antiserum (BD, Allschwil, Switzerland, 1:200) in PBS‐T with 10% of goat serum for 1 hr at RT. .. Coverslips were then washed three times in PBS‐T and then incubated with Alexa Fluor 488‐ conjugated goat anti‐rabbit IgG secondary antibody (Life technologies, 1:500) and with DAPI (Invitrogen, Carlsbad, CA, USA, T3604, 1:10,000) for one hour in the dark.

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    Agilent technologies pbs t
    Pbs T, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Agilent technologies
    Average 94 stars, based on 516 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    94/100 stars
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    Agilent technologies buffer pbs t
    Buffer Pbs T, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer pbs t/product/Agilent technologies
    Average 93 stars, based on 1 article reviews
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    buffer pbs t - by Bioz Stars, 2020-07
    93/100 stars
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