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tiangen biotech co pbs t vector
Pbs T Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t vector/product/tiangen biotech co
Average 88 stars, based on 7 article reviews
Price from $9.99 to $1999.99
pbs t vector - by Bioz Stars, 2020-07
88/100 stars

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Sequencing:

Article Title: Comparative Analysis of the Mitochondrial Genomes of Callitettixini Spittlebugs (Hemiptera: Cercopidae) Confirms the Overall High Evolutionary Speed of the AT-Rich Region but Reveals the Presence of Short Conservative Elements at the Tribal Level
Article Snippet: .. When the direct sequencing of the PCR product did not yield successful results, the target bands were cloned into pBS-T Vector (Tiangen) for sequencing. .. The sequencing was conducted using a PE/ABI 377 automated sequencer with the ABI PRISM BigDye Terminator Cycle Sequencing v 2.0 Ready Reaction Kit (PE Biosystems) following the manufacturers protocols.

Article Title: Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells
Article Snippet: .. The ALDH2 cDNA was cloned with DNA polymerase (TaKaRa, Japan) into pBS-T vector (TIANGEN, China) and sent to Jinsite Bio Inc (China) for sequencing. .. The primers used in PCR were ALDH2 forward 5′-GACACGGATCCATGTTGCGCGCTGCCGCCCGCTTCGG-3′ and ALDH2 reverse 5′-GACACGAATTCTTATGAGTTCTTCTGAGGCACTTTGAC-3′.

Polymerase Chain Reaction:

Article Title: Comparative Analysis of the Mitochondrial Genomes of Callitettixini Spittlebugs (Hemiptera: Cercopidae) Confirms the Overall High Evolutionary Speed of the AT-Rich Region but Reveals the Presence of Short Conservative Elements at the Tribal Level
Article Snippet: .. When the direct sequencing of the PCR product did not yield successful results, the target bands were cloned into pBS-T Vector (Tiangen) for sequencing. .. The sequencing was conducted using a PE/ABI 377 automated sequencer with the ABI PRISM BigDye Terminator Cycle Sequencing v 2.0 Ready Reaction Kit (PE Biosystems) following the manufacturers protocols.

Article Title: Miiuy Croaker Transferrin Gene and Evidence for Positive Selection Events Reveal Different Evolutionary Patterns
Article Snippet: .. The PCR products were then cloned into PBS-T vector (TIANGEN). .. At least three clones were sequenced by the M13F/R sequencing primers.

Plasmid Preparation:

Article Title: Comparative Analysis of the Mitochondrial Genomes of Callitettixini Spittlebugs (Hemiptera: Cercopidae) Confirms the Overall High Evolutionary Speed of the AT-Rich Region but Reveals the Presence of Short Conservative Elements at the Tribal Level
Article Snippet: .. When the direct sequencing of the PCR product did not yield successful results, the target bands were cloned into pBS-T Vector (Tiangen) for sequencing. .. The sequencing was conducted using a PE/ABI 377 automated sequencer with the ABI PRISM BigDye Terminator Cycle Sequencing v 2.0 Ready Reaction Kit (PE Biosystems) following the manufacturers protocols.

Article Title: Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells
Article Snippet: .. The ALDH2 cDNA was cloned with DNA polymerase (TaKaRa, Japan) into pBS-T vector (TIANGEN, China) and sent to Jinsite Bio Inc (China) for sequencing. .. The primers used in PCR were ALDH2 forward 5′-GACACGGATCCATGTTGCGCGCTGCCGCCCGCTTCGG-3′ and ALDH2 reverse 5′-GACACGAATTCTTATGAGTTCTTCTGAGGCACTTTGAC-3′.

Article Title: Miiuy Croaker Transferrin Gene and Evidence for Positive Selection Events Reveal Different Evolutionary Patterns
Article Snippet: .. The PCR products were then cloned into PBS-T vector (TIANGEN). .. At least three clones were sequenced by the M13F/R sequencing primers.

Article Title: Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation
Article Snippet: .. The retrieved H-2 DNA fragments of each locus, K, D, L, Aα, Aβ, Eα and Eβ were cloned into the pBS-T vector (50 ng/µl; Tiangen Biotech Co., Ltd.). .. Escherichia coli ( E. coli ) TOP-10 cells (Tiangen Biotech Co., Ltd.) were transformed using recombinant DNA and vector.

Clone Assay:

Article Title: Comparative Analysis of the Mitochondrial Genomes of Callitettixini Spittlebugs (Hemiptera: Cercopidae) Confirms the Overall High Evolutionary Speed of the AT-Rich Region but Reveals the Presence of Short Conservative Elements at the Tribal Level
Article Snippet: .. When the direct sequencing of the PCR product did not yield successful results, the target bands were cloned into pBS-T Vector (Tiangen) for sequencing. .. The sequencing was conducted using a PE/ABI 377 automated sequencer with the ABI PRISM BigDye Terminator Cycle Sequencing v 2.0 Ready Reaction Kit (PE Biosystems) following the manufacturers protocols.

Article Title: Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells
Article Snippet: .. The ALDH2 cDNA was cloned with DNA polymerase (TaKaRa, Japan) into pBS-T vector (TIANGEN, China) and sent to Jinsite Bio Inc (China) for sequencing. .. The primers used in PCR were ALDH2 forward 5′-GACACGGATCCATGTTGCGCGCTGCCGCCCGCTTCGG-3′ and ALDH2 reverse 5′-GACACGAATTCTTATGAGTTCTTCTGAGGCACTTTGAC-3′.

Article Title: Miiuy Croaker Transferrin Gene and Evidence for Positive Selection Events Reveal Different Evolutionary Patterns
Article Snippet: .. The PCR products were then cloned into PBS-T vector (TIANGEN). .. At least three clones were sequenced by the M13F/R sequencing primers.

Article Title: Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation
Article Snippet: .. The retrieved H-2 DNA fragments of each locus, K, D, L, Aα, Aβ, Eα and Eβ were cloned into the pBS-T vector (50 ng/µl; Tiangen Biotech Co., Ltd.). .. Escherichia coli ( E. coli ) TOP-10 cells (Tiangen Biotech Co., Ltd.) were transformed using recombinant DNA and vector.

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