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Merck KGaA pbs solution
Pbs Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs solution/product/Merck KGaA
Average 92 stars, based on 8 article reviews
Price from $9.99 to $1999.99
pbs solution - by Bioz Stars, 2020-09
92/100 stars

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TUNEL Assay:

Article Title: Generation of two-cell cloned embryos from mouse faecal cell
Article Snippet: .. The cloned embryos at 8 hpa were fixed by 4% PFA and washed twice in PBS solution containing 30 mg/mL BSA; then, a TUNEL analysis was performed using In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) following the manufacturer’s recommendations. .. Specimens were mounted on glass slides in Vectashield mounting medium with DAPI.

Clone Assay:

Article Title: Generation of two-cell cloned embryos from mouse faecal cell
Article Snippet: .. The cloned embryos at 8 hpa were fixed by 4% PFA and washed twice in PBS solution containing 30 mg/mL BSA; then, a TUNEL analysis was performed using In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) following the manufacturer’s recommendations. .. Specimens were mounted on glass slides in Vectashield mounting medium with DAPI.

In Situ:

Article Title: Generation of two-cell cloned embryos from mouse faecal cell
Article Snippet: .. The cloned embryos at 8 hpa were fixed by 4% PFA and washed twice in PBS solution containing 30 mg/mL BSA; then, a TUNEL analysis was performed using In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) following the manufacturer’s recommendations. .. Specimens were mounted on glass slides in Vectashield mounting medium with DAPI.

Western Blot:

Article Title: Uncoupling of EGFR–RAS signaling and nuclear localization of YBX1 in colorectal cancer
Article Snippet: .. Western blot About 40 μg of whole cell lysates per lane were separated by SDS–PAGE at 100mA for 60 min. LI-COR blocking buffer (LI-COR Biosciences, Lincoln, NE, USA) and PBS solution was applied 1:1 for 60 min. We used Pan-RAS primary antibody (mouse monoclonal Anti-Pan-Ras, MABS195; dilution 1:500; Merck Millipore, Merck KGaA, Darmstadt, Germany) for detection of total RAS protein. ..

Blocking Assay:

Article Title: Uncoupling of EGFR–RAS signaling and nuclear localization of YBX1 in colorectal cancer
Article Snippet: .. Western blot About 40 μg of whole cell lysates per lane were separated by SDS–PAGE at 100mA for 60 min. LI-COR blocking buffer (LI-COR Biosciences, Lincoln, NE, USA) and PBS solution was applied 1:1 for 60 min. We used Pan-RAS primary antibody (mouse monoclonal Anti-Pan-Ras, MABS195; dilution 1:500; Merck Millipore, Merck KGaA, Darmstadt, Germany) for detection of total RAS protein. ..

Article Title: Transient Receptor Potential Vanilloid 1 Expression Mediates Capsaicin-Induced Cell Death
Article Snippet: .. HeLa cells were collected by trypsinization and fixed with 4% paraformaldehyde for 30 min. Next, the cells were blocked and permeabilized using a PBS solution with 5% bovine serum albumin (Merck KGaA, Darmstadt, Germany) and 2% Tween-20 (Merck KGaA, Darmstadt, Germany) for 1 h and then incubated with an anti-TRPV1 antibody (Santa Cruz Biotechnology, Inc., United States, 1:200) in blocking solution overnight at 4°C. .. After washing with PBS, the cells were incubated with anti-goat biotinylated secondary antibody (Jackson ImmunoResearch, United States, 1:500) for 1 h at 37°C.

SYBR Green Assay:

Article Title: Lactobacillus kefiranofaciens, the sole dominant and stable bacterial species, exhibits distinct morphotypes upon colonization in Tibetan kefir grains
Article Snippet: .. The cell pellets were re-suspended in PBS solution and was fixed in 4% of formaldehyde for 14 h. The fixed L. kefiranofaciens cells were filtered through a 0.2 μm GTTP membrane (Merck Millipore), stained with 1 × SYBR Green I (Solarbio, Beijing, China) for 15 min, and observed under an epifluorescence microscope (Zeiss Axiophot, Germany). ..

Incubation:

Article Title: Identification of Metabolic Pathways Influenced by the G-Protein Coupled Receptors GprB and GprD in Aspergillus nidulans
Article Snippet: .. Five plates for each strain were incubated for 48 h, and the conidia produced on each plate were harvested with 10 ml of PBS solution containing 2% (v/v) Tween 80 (Merck, Germany). .. The spore suspensions were filtered through 40 µm-pore filter (Millipore, USA), and the number of conidia was determined using a counting chamber.

Article Title: NGAL protects in nasopharyngeal carcinoma by inducing apoptosis and blocking epithelial-mesenchymal transition
Article Snippet: .. After 48 h treatment with 0.5% bovine serum albumin (Sigma-Aldrich; Merck KGaA) PBS solution, the harvested cells were washed with cold PBS and then suspended in 200 ml combined buffer (10 mM HEPES/NaOH pH 7.4, 140 mM NaCl and 2.5 mM CaCl2 ) and incubated together. ..

Article Title: Transient Receptor Potential Vanilloid 1 Expression Mediates Capsaicin-Induced Cell Death
Article Snippet: .. HeLa cells were collected by trypsinization and fixed with 4% paraformaldehyde for 30 min. Next, the cells were blocked and permeabilized using a PBS solution with 5% bovine serum albumin (Merck KGaA, Darmstadt, Germany) and 2% Tween-20 (Merck KGaA, Darmstadt, Germany) for 1 h and then incubated with an anti-TRPV1 antibody (Santa Cruz Biotechnology, Inc., United States, 1:200) in blocking solution overnight at 4°C. .. After washing with PBS, the cells were incubated with anti-goat biotinylated secondary antibody (Jackson ImmunoResearch, United States, 1:500) for 1 h at 37°C.

Microscopy:

Article Title: Lactobacillus kefiranofaciens, the sole dominant and stable bacterial species, exhibits distinct morphotypes upon colonization in Tibetan kefir grains
Article Snippet: .. The cell pellets were re-suspended in PBS solution and was fixed in 4% of formaldehyde for 14 h. The fixed L. kefiranofaciens cells were filtered through a 0.2 μm GTTP membrane (Merck Millipore), stained with 1 × SYBR Green I (Solarbio, Beijing, China) for 15 min, and observed under an epifluorescence microscope (Zeiss Axiophot, Germany). ..

Staining:

Article Title: Lactobacillus kefiranofaciens, the sole dominant and stable bacterial species, exhibits distinct morphotypes upon colonization in Tibetan kefir grains
Article Snippet: .. The cell pellets were re-suspended in PBS solution and was fixed in 4% of formaldehyde for 14 h. The fixed L. kefiranofaciens cells were filtered through a 0.2 μm GTTP membrane (Merck Millipore), stained with 1 × SYBR Green I (Solarbio, Beijing, China) for 15 min, and observed under an epifluorescence microscope (Zeiss Axiophot, Germany). ..

Produced:

Article Title: Identification of Metabolic Pathways Influenced by the G-Protein Coupled Receptors GprB and GprD in Aspergillus nidulans
Article Snippet: .. Five plates for each strain were incubated for 48 h, and the conidia produced on each plate were harvested with 10 ml of PBS solution containing 2% (v/v) Tween 80 (Merck, Germany). .. The spore suspensions were filtered through 40 µm-pore filter (Millipore, USA), and the number of conidia was determined using a counting chamber.

Binding Assay:

Article Title: A common mechanism allows selective targeting of GluN2B subunit-containing N-methyl-D-aspartate receptors
Article Snippet: .. For binding assay, cell pellet was resuspended in PBS solution and number of cells was determined using a Scepter® cell counter (MERCK Millipore, Darmstadt, Germany). .. Cell fragments were generated by sonication and centrifugation (23,500 × g , 4 °C) resulting in fragments of ~500,000 cell/mL (23,500 × g , 4 °C).

SDS Page:

Article Title: Uncoupling of EGFR–RAS signaling and nuclear localization of YBX1 in colorectal cancer
Article Snippet: .. Western blot About 40 μg of whole cell lysates per lane were separated by SDS–PAGE at 100mA for 60 min. LI-COR blocking buffer (LI-COR Biosciences, Lincoln, NE, USA) and PBS solution was applied 1:1 for 60 min. We used Pan-RAS primary antibody (mouse monoclonal Anti-Pan-Ras, MABS195; dilution 1:500; Merck Millipore, Merck KGaA, Darmstadt, Germany) for detection of total RAS protein. ..

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  • 90
    Merck KGaA phosphate buffered saline pbs solution blot membrane
    Phosphate Buffered Saline Pbs Solution Blot Membrane, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution blot membrane/product/Merck KGaA
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution blot membrane - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    Merck KGaA phosphate buffered saline pbs solution
    Phosphate Buffered Saline Pbs Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered saline pbs solution/product/Merck KGaA
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered saline pbs solution - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    Merck KGaA phosphate buffer solution pbs
    Phosphate Buffer Solution Pbs, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffer solution pbs/product/Merck KGaA
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer solution pbs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Merck KGaA pbs solution
    Pbs Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Merck KGaA
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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