Structured Review

GE Healthcare pbs solution
Effect of low or high concentration of <t>AECS/p-BQ</t> on reactive oxygen species (ROS) production in cultured A549 cells and its prevention by vit C. Serum-starved A549 cells grown on coverslips were either nontreated (NT) or exposed to 2 μ L/mL AECS, 50 μ L/mL AECS, 200 ng/mL p-BQ or 2.5 μ g/mL p-BQ for 1 hr with or without vitamin C (40 μ g/mL) pretreatment in serum-free medium for 15 min. After treatment, cells were incubated in fresh media containing H 2 DCFDA for 30 min and <t>PBS</t> washed twice, and fluorescent images were captured.
Pbs Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs solution/product/GE Healthcare
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pbs solution - by Bioz Stars, 2020-09
99/100 stars

Images

1) Product Images from "Molecular Mechanisms of Cigarette Smoke-Induced Proliferation of Lung Cells and Prevention by Vitamin C"

Article Title: Molecular Mechanisms of Cigarette Smoke-Induced Proliferation of Lung Cells and Prevention by Vitamin C

Journal: Journal of Oncology

doi: 10.1155/2011/561862

Effect of low or high concentration of AECS/p-BQ on reactive oxygen species (ROS) production in cultured A549 cells and its prevention by vit C. Serum-starved A549 cells grown on coverslips were either nontreated (NT) or exposed to 2 μ L/mL AECS, 50 μ L/mL AECS, 200 ng/mL p-BQ or 2.5 μ g/mL p-BQ for 1 hr with or without vitamin C (40 μ g/mL) pretreatment in serum-free medium for 15 min. After treatment, cells were incubated in fresh media containing H 2 DCFDA for 30 min and PBS washed twice, and fluorescent images were captured.
Figure Legend Snippet: Effect of low or high concentration of AECS/p-BQ on reactive oxygen species (ROS) production in cultured A549 cells and its prevention by vit C. Serum-starved A549 cells grown on coverslips were either nontreated (NT) or exposed to 2 μ L/mL AECS, 50 μ L/mL AECS, 200 ng/mL p-BQ or 2.5 μ g/mL p-BQ for 1 hr with or without vitamin C (40 μ g/mL) pretreatment in serum-free medium for 15 min. After treatment, cells were incubated in fresh media containing H 2 DCFDA for 30 min and PBS washed twice, and fluorescent images were captured.

Techniques Used: Concentration Assay, Cell Culture, Incubation

EGFR exposed to AECS/p-BQ cannot bind c-Cbl and is not ubiquitinated. Serum-starved A549 cells were either nontreated (NT) or exposed to 100 ng/mL EGF for 5 min (used as positive control), 2 μ L/mL AECS or 200 ng/mL p-BQ for 1 hr. After treatment, cells were immediately lysed, and the EGFR was immunoprecipitated (IP) from the cell lysates using anti-EGFR antibody. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted (IB) with the indicated antibodies (a). Serum-starved A549 cells were either nontreated (NT) or exposed to 100 ng/mL EGF for 5 min or 2 hr, 2 μ L/mL AECS or 200 ng/mL p-BQ for 1 hr. After treatment, cells were washed with PBS and further incubated in fresh serum-free medium for 2 hr at 37°C before lysis. The EGFR was immunoprecipitated (IP) from the cell lysates using anti-EGFR antibody. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted (IB) with antiphosphotyrosine (PY20) antibody (b).
Figure Legend Snippet: EGFR exposed to AECS/p-BQ cannot bind c-Cbl and is not ubiquitinated. Serum-starved A549 cells were either nontreated (NT) or exposed to 100 ng/mL EGF for 5 min (used as positive control), 2 μ L/mL AECS or 200 ng/mL p-BQ for 1 hr. After treatment, cells were immediately lysed, and the EGFR was immunoprecipitated (IP) from the cell lysates using anti-EGFR antibody. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted (IB) with the indicated antibodies (a). Serum-starved A549 cells were either nontreated (NT) or exposed to 100 ng/mL EGF for 5 min or 2 hr, 2 μ L/mL AECS or 200 ng/mL p-BQ for 1 hr. After treatment, cells were washed with PBS and further incubated in fresh serum-free medium for 2 hr at 37°C before lysis. The EGFR was immunoprecipitated (IP) from the cell lysates using anti-EGFR antibody. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to PVDF membrane, and immunoblotted (IB) with antiphosphotyrosine (PY20) antibody (b).

Techniques Used: Positive Control, Immunoprecipitation, SDS Page, Incubation, Lysis

Related Articles

GST Pulldown Assay:

Article Title: HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner
Article Snippet: .. GST pulldown assay Purified His-Bat3 protein and Flag-HAUSP-truncated proteins were incubated with GST-tagged p53 protein or GST control protein in PBS buffer with glutathione agarose (GE Healthcare) for 2 h at 4°C. .. The incubated proteins were then washed and immunoblotted using anti-His, anti-Flag, or anti-GST antibodies.

Filtration:

Article Title: Expression and Characterization of Cryptococcus neoformans Recombinant App1
Article Snippet: .. One hundred microlitre of a 1 mg/ml solution of rApp1 dialyzed into PBS buffer was injected onto a Superdex 75 10/300 GL gel filtration column at 10°C (GE Healthcare Cat # 17-5174-01) using an AKTA fast-performance liquid chromatography system (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS) supplemented with 137 mM NaCl, 2.7 mM KCl, 10 mM Na2 HPO4 , and 2 mM KH2 PO4 pH 7.6. .. The molecular weight markers comprised a mixture of aprotinin (6.5 kDa), ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), and conalbumin (75 kDa).

In Vitro:

Article Title: Magnetic Field Promotes Migration of Schwann Cells with Chondroitinase ABC (ChABC)-Loaded Superparamagnetic Nanoparticles Across Astrocyte Boundary in vitro
Article Snippet: .. Cryostat sections were incubated in PBS (HyClone), in conditioned medium collected from PEI-SPIONs/ChABC/SCs cultures, or in PBS (HyClone) containing ChABC (300 mU/mL) for 3 hrs at 37°C to confirm the bioactivity of ChABC to degrade CSPGs in the spinal cord tissue., Moreover, the ability of astrocytes to express CSPGs in vitro was also demonstrated. .. Immunostaining of the incubated sections or astrocytes for CS-56 and GFAP was performed as described in the immunofluorescence staining subsection below.

Size-exclusion Chromatography:

Article Title: Outer Membrane Vesicles Induce Immune Responses to Virulence Proteins and Protect against Colonization by Enterotoxigenic Escherichia coli ▿
Article Snippet: .. CexE-His6 was further purified and then exchanged into PBS buffer (pH 7.4) by size exclusion chromatography (Superdex 200 10/300 GL column; GE Healthcare). .. Heat-labile toxin was obtained from List Biological Laboratories, Campbell, CA.

Purification:

Article Title: Outer Membrane Vesicles Induce Immune Responses to Virulence Proteins and Protect against Colonization by Enterotoxigenic Escherichia coli ▿
Article Snippet: .. CexE-His6 was further purified and then exchanged into PBS buffer (pH 7.4) by size exclusion chromatography (Superdex 200 10/300 GL column; GE Healthcare). .. Heat-labile toxin was obtained from List Biological Laboratories, Campbell, CA.

Article Title: HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner
Article Snippet: .. GST pulldown assay Purified His-Bat3 protein and Flag-HAUSP-truncated proteins were incubated with GST-tagged p53 protein or GST control protein in PBS buffer with glutathione agarose (GE Healthcare) for 2 h at 4°C. .. The incubated proteins were then washed and immunoblotted using anti-His, anti-Flag, or anti-GST antibodies.

Concentration Assay:

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: .. The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Incubation:

Article Title: HLA-B-associated transcript 3 (Bat3) stabilizes and activates p53 in a HAUSP-dependent manner
Article Snippet: .. GST pulldown assay Purified His-Bat3 protein and Flag-HAUSP-truncated proteins were incubated with GST-tagged p53 protein or GST control protein in PBS buffer with glutathione agarose (GE Healthcare) for 2 h at 4°C. .. The incubated proteins were then washed and immunoblotted using anti-His, anti-Flag, or anti-GST antibodies.

Article Title: Enhanced Expression and Functional Characterization of the Recombinant Putative Lysozyme-PMAP36 Fusion Protein
Article Snippet: .. The cell cultures at mid-logarithmic phase, OD600 of 0.2, were mixed with PBS (GE Healthcare) and PMAP36-P22 lysozyme fusion protein (final concentration: 64 μM) and incubated for 10 min at 37°C. .. We added EtBr (final concentration: 6 μM) to the reaction mixture and measured the fluorescence using fluorescence spectrometer (Infinite 200® Pro, TECAN, Austria); the excitation and emission wavelengths were 545 and 600 nm, respectively.

Article Title: Magnetic Field Promotes Migration of Schwann Cells with Chondroitinase ABC (ChABC)-Loaded Superparamagnetic Nanoparticles Across Astrocyte Boundary in vitro
Article Snippet: .. Cryostat sections were incubated in PBS (HyClone), in conditioned medium collected from PEI-SPIONs/ChABC/SCs cultures, or in PBS (HyClone) containing ChABC (300 mU/mL) for 3 hrs at 37°C to confirm the bioactivity of ChABC to degrade CSPGs in the spinal cord tissue., Moreover, the ability of astrocytes to express CSPGs in vitro was also demonstrated. .. Immunostaining of the incubated sections or astrocytes for CS-56 and GFAP was performed as described in the immunofluorescence staining subsection below.

Injection:

Article Title: Expression and Characterization of Cryptococcus neoformans Recombinant App1
Article Snippet: .. One hundred microlitre of a 1 mg/ml solution of rApp1 dialyzed into PBS buffer was injected onto a Superdex 75 10/300 GL gel filtration column at 10°C (GE Healthcare Cat # 17-5174-01) using an AKTA fast-performance liquid chromatography system (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS) supplemented with 137 mM NaCl, 2.7 mM KCl, 10 mM Na2 HPO4 , and 2 mM KH2 PO4 pH 7.6. .. The molecular weight markers comprised a mixture of aprotinin (6.5 kDa), ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), and conalbumin (75 kDa).

Binding Assay:

Article Title: TERT alleviates irradiation-induced late rectal injury by reducing hypoxia-induced ROS levels through the activation of NF-κB and autophagy
Article Snippet: .. Detection of apoptosis The digested cells were washed twice with PBS (pH 7.4; HyClone), centrifuged for 5 min at 37°C, and resuspended in binding buffer. .. They were then stained using the Annexin V-FITC Apoptosis Detection kit, followed by the addition of propidium iodide (PI) (both from BD Biosciences, San Jose, CA, USA).

Liquid Chromatography:

Article Title: Expression and Characterization of Cryptococcus neoformans Recombinant App1
Article Snippet: .. One hundred microlitre of a 1 mg/ml solution of rApp1 dialyzed into PBS buffer was injected onto a Superdex 75 10/300 GL gel filtration column at 10°C (GE Healthcare Cat # 17-5174-01) using an AKTA fast-performance liquid chromatography system (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS) supplemented with 137 mM NaCl, 2.7 mM KCl, 10 mM Na2 HPO4 , and 2 mM KH2 PO4 pH 7.6. .. The molecular weight markers comprised a mixture of aprotinin (6.5 kDa), ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), ovalbumin (43 kDa), and conalbumin (75 kDa).

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